Click here to switch to the map view.

The map label for this gene is pncA [H]

Identifier: 50086536

GI number: 50086536

Start: 3503224

End: 3503868

Strand: Reverse

Name: pncA [H]

Synonym: ACIAD3584

Alternate gene names: 50086536

Gene position: 3503868-3503224 (Counterclockwise)

Preceding gene: 50086545

Following gene: 50086535

Centisome position: 97.37

GC content: 44.34

Gene sequence:

>645_bases
ATGCTGGAGCTTAGAATGAAACAGGCAAACAATTGCGCGTTGATCGTGGTGGATGTGCAAAATGGATTTACGCCAGGCGG
TAATTTGGCGGTTGCTGGTGCGGATCAGATTATTCCATGCATTAATCAGTTGGGTACCTGTTTTGATACTATTGTGATTA
CACAGGACTGGCATCCACACAATCATATCTCTTTTGCTAGTAATCATCTGGGAAAACAGCCATTTGATACCATTCAATTG
CCTTATGGCCCGCAAGTATTATGGCCAAGCCATTGTGTTCAGGGTACTCAGGATGCCGAGTTACATCCTGCACTTGATTT
ACCCACGGCACAATTGATTATTCGCAAAGGTTTTCATCGCAATATCGACAGTTACTCAGCTTTTATGGAGGCGGATCGGC
ATACCTCGACGGGTCTGGCAGGCTACTTAAAAGAGCGGGGCATTGATACAGTTTATATTGTGGGGATTGCAACTGATTTT
TGTGTGGCATGGACAGCAATTGATGCCTCTAAGGCAGGACTAAACAGTTATGTCATTATAGATGCCTGTAAAGCCATCGA
TATGAATGGCTCGCTACAACATGCTTGGCAGGAAATGCTGGCATCGGGCGTTCAACGTATTTCATCACGCAATATTTTGG
TTTAA

Upstream 100 bases:

>100_bases
TCATGATGTCGAGTTGCTATGCTGCGATGTGGTGAGAAAACGTAATCAATCACAACAATAAGATCCTTCTGTCTAGGTCG
ATAATATTTAAGGCAGAATG

Downstream 100 bases:

>100_bases
GGTACGCACCCTTACGGATTAATGCAAATTGTAAAAATTAAGATGGAGCGCGGACGAATGATGGCATTGTTGCGATTTAC
CGCTTTTGTGCAAAAAACCT

Product: bifunctional pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MLELRMKQANNCALIVVDVQNGFTPGGNLAVAGADQIIPCINQLGTCFDTIVITQDWHPHNHISFASNHLGKQPFDTIQL
PYGPQVLWPSHCVQGTQDAELHPALDLPTAQLIIRKGFHRNIDSYSAFMEADRHTSTGLAGYLKERGIDTVYIVGIATDF
CVAWTAIDASKAGLNSYVIIDACKAIDMNGSLQHAWQEMLASGVQRISSRNILV

Sequences:

>Translated_214_residues
MLELRMKQANNCALIVVDVQNGFTPGGNLAVAGADQIIPCINQLGTCFDTIVITQDWHPHNHISFASNHLGKQPFDTIQL
PYGPQVLWPSHCVQGTQDAELHPALDLPTAQLIIRKGFHRNIDSYSAFMEADRHTSTGLAGYLKERGIDTVYIVGIATDF
CVAWTAIDASKAGLNSYVIIDACKAIDMNGSLQHAWQEMLASGVQRISSRNILV
>Mature_214_residues
MLELRMKQANNCALIVVDVQNGFTPGGNLAVAGADQIIPCINQLGTCFDTIVITQDWHPHNHISFASNHLGKQPFDTIQL
PYGPQVLWPSHCVQGTQDAELHPALDLPTAQLIIRKGFHRNIDSYSAFMEADRHTSTGLAGYLKERGIDTVYIVGIATDF
CVAWTAIDASKAGLNSYVIIDACKAIDMNGSLQHAWQEMLASGVQRISSRNILV

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=196, Percent_Identity=39.7959183673469, Blast_Score=146, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI193206735, Length=222, Percent_Identity=32.8828828828829, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17543130, Length=221, Percent_Identity=33.0316742081448, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71999339, Length=198, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6321401, Length=210, Percent_Identity=35.2380952380952, Blast_Score=116, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648247, Length=214, Percent_Identity=33.6448598130841, Blast_Score=111, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 23413; Mature: 23413

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELRMKQANNCALIVVDVQNGFTPGGNLAVAGADQIIPCINQLGTCFDTIVITQDWHPH
CCEEEEECCCCEEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHEEEEECCCCCC
NHISFASNHLGKQPFDTIQLPYGPQVLWPSHCVQGTQDAELHPALDLPTAQLIIRKGFHR
CCCCHHHHHCCCCCCCEEECCCCCCEECHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
NIDSYSAFMEADRHTSTGLAGYLKERGIDTVYIVGIATDFCVAWTAIDASKAGLNSYVII
CHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCHHCCCCEEEEE
DACKAIDMNGSLQHAWQEMLASGVQRISSRNILV
EEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLELRMKQANNCALIVVDVQNGFTPGGNLAVAGADQIIPCINQLGTCFDTIVITQDWHPH
CCEEEEECCCCEEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHEEEEECCCCCC
NHISFASNHLGKQPFDTIQLPYGPQVLWPSHCVQGTQDAELHPALDLPTAQLIIRKGFHR
CCCCHHHHHCCCCCCCEEECCCCCCEECHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
NIDSYSAFMEADRHTSTGLAGYLKERGIDTVYIVGIATDFCVAWTAIDASKAGLNSYVII
CHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCHHCCCCEEEEE
DACKAIDMNGSLQHAWQEMLASGVQRISSRNILV
EEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]