Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 50086511

GI number: 50086511

Start: 3482534

End: 3483244

Strand: Direct

Name: phoB [H]

Synonym: ACIAD3557

Alternate gene names: 50086511

Gene position: 3482534-3483244 (Clockwise)

Preceding gene: 50086510

Following gene: 50086512

Centisome position: 96.77

GC content: 45.01

Gene sequence:

>711_bases
GTGAAAGATGACTATATTTTAATCGTCGATGATGAATTACCCATCCGCGAAATGATCCATACCTCACTGGATATGGCAGG
CTTTCAATGTTTACAGGCTGAAGATGCCAAACAGGCGCATCAAATGATCGTTGATCAACGACCTGCCCTGATTTTACTCG
ATTGGATGTTGCCTGGTGGCGTCAGTGGTGTCGATTTATGTCGCCGTTTAAAACGTGATGAAAATCTTTCTGAAATTCCA
GTGATTATGTTAACCGCTCGTGGAGAAGAAGATCATAAGGTTCAAGGTCTGGATGCAGGCGCCGATGACTATATGACCAA
GCCGTTTTCTACACGCGAACTTGTATCGCGTATCAAGGCTGTATTACGTCGTGCTAACGCACTAAACGGCGATAAAGTGA
TTGATGCCAATGGCTTGATTCTTGACCCTGTGAGTCAGCGTGTGAGTTTTGGCAGCAATATCTTGGATATGGGGCCAACA
GAATATCGCTTACTGGCATTTTTTATGACGCATCCTGAACGTGCCTATACCCGTGCACAATTACTGGATCAGGTATGGGG
TGGCAATGTCTATATCGAAGATCGTACCATTGATGTACATATCCGTCGTTTACGTAAAGTTTTAGAACCTTTTGGCGCCG
ACCGTTTTGTACAAACCGTTCGTGGCACAGGTTACCGTTTTTCTACCCGAGCAGATTTAGCAGCAGGCTAA

Upstream 100 bases:

>100_bases
ATCCTTTTTTTGCAATGATACTGTCATAATTTATCTTTGTAATAAGCCTGTCATAAATAAAAGCGGTTCACCGTTTAAAA
CATCTATAGGATATTGCGTT

Downstream 100 bases:

>100_bases
CCCTTGACTATGTATGAACCCTATCCTGTACCCGAACTGGCACGTGAGCATAAACGCTTTCGTTATAGCAGTTTGTGGAC
TTTTGCCAAACAGGACTTAC

Product: positive response regulator for the pho regulon,autophosphorylates and phosphorylates sensor PhoR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKDDYILIVDDELPIREMIHTSLDMAGFQCLQAEDAKQAHQMIVDQRPALILLDWMLPGGVSGVDLCRRLKRDENLSEIP
VIMLTARGEEDHKVQGLDAGADDYMTKPFSTRELVSRIKAVLRRANALNGDKVIDANGLILDPVSQRVSFGSNILDMGPT
EYRLLAFFMTHPERAYTRAQLLDQVWGGNVYIEDRTIDVHIRRLRKVLEPFGADRFVQTVRGTGYRFSTRADLAAG

Sequences:

>Translated_236_residues
MKDDYILIVDDELPIREMIHTSLDMAGFQCLQAEDAKQAHQMIVDQRPALILLDWMLPGGVSGVDLCRRLKRDENLSEIP
VIMLTARGEEDHKVQGLDAGADDYMTKPFSTRELVSRIKAVLRRANALNGDKVIDANGLILDPVSQRVSFGSNILDMGPT
EYRLLAFFMTHPERAYTRAQLLDQVWGGNVYIEDRTIDVHIRRLRKVLEPFGADRFVQTVRGTGYRFSTRADLAAG
>Mature_236_residues
MKDDYILIVDDELPIREMIHTSLDMAGFQCLQAEDAKQAHQMIVDQRPALILLDWMLPGGVSGVDLCRRLKRDENLSEIP
VIMLTARGEEDHKVQGLDAGADDYMTKPFSTRELVSRIKAVLRRANALNGDKVIDANGLILDPVSQRVSFGSNILDMGPT
EYRLLAFFMTHPERAYTRAQLLDQVWGGNVYIEDRTIDVHIRRLRKVLEPFGADRFVQTVRGTGYRFSTRADLAAG

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=62.2222222222222, Blast_Score=289, Evalue=1e-79,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=38.1578947368421, Blast_Score=147, Evalue=5e-37,
Organism=Escherichia coli, GI1789809, Length=235, Percent_Identity=37.0212765957447, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=36, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=35.4545454545455, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI2367329, Length=231, Percent_Identity=38.5281385281385, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=35.8744394618834, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1790863, Length=237, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=33.920704845815, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=31.5555555555556, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=29.7777777777778, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=33.1818181818182, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787229, Length=228, Percent_Identity=31.5789473684211, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI145693140, Length=240, Percent_Identity=27.9166666666667, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788550, Length=111, Percent_Identity=34.2342342342342, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1790299, Length=146, Percent_Identity=26.027397260274, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788191, Length=124, Percent_Identity=26.6129032258064, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26659; Mature: 26659

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDDYILIVDDELPIREMIHTSLDMAGFQCLQAEDAKQAHQMIVDQRPALILLDWMLPGG
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
VSGVDLCRRLKRDENLSEIPVIMLTARGEEDHKVQGLDAGADDYMTKPFSTRELVSRIKA
CCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCCCHHHCCCCCCHHHHHHHHHH
VLRRANALNGDKVIDANGLILDPVSQRVSFGSNILDMGPTEYRLLAFFMTHPERAYTRAQ
HHHHHCCCCCCEEECCCCEEECCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LLDQVWGGNVYIEDRTIDVHIRRLRKVLEPFGADRFVQTVRGTGYRFSTRADLAAG
HHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCC
>Mature Secondary Structure
MKDDYILIVDDELPIREMIHTSLDMAGFQCLQAEDAKQAHQMIVDQRPALILLDWMLPGG
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
VSGVDLCRRLKRDENLSEIPVIMLTARGEEDHKVQGLDAGADDYMTKPFSTRELVSRIKA
CCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCCCHHHCCCCCCHHHHHHHHHH
VLRRANALNGDKVIDANGLILDPVSQRVSFGSNILDMGPTEYRLLAFFMTHPERAYTRAQ
HHHHHCCCCCCEEECCCCEEECCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LLDQVWGGNVYIEDRTIDVHIRRLRKVLEPFGADRFVQTVRGTGYRFSTRADLAAG
HHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]