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The map label for this gene is rutF [H]

Identifier: 50086478

GI number: 50086478

Start: 3450070

End: 3450576

Strand: Reverse

Name: rutF [H]

Synonym: ACIAD3522

Alternate gene names: 50086478

Gene position: 3450576-3450070 (Counterclockwise)

Preceding gene: 50086479

Following gene: 50086475

Centisome position: 95.89

GC content: 42.8

Gene sequence:

>507_bases
GTGATGATTGAAACAACAGACTTTAGAAATGCAATGTCCTTATTGAGCAGTGCAGTCAGTGTTGTCACTACAGCAGGTTT
AGCGGGTCGTTATGGCTTTACTGCATCTGCGGTATGCAGTGTCACAGATACGCCTCCGACATTGCTGGTATGTATGAATC
AGTCATCTAGCTCACATATGCATTTTGTAGAAAACAAGATTTTAACCGTGAATGTGCTTAGTGCGCAGCATCAGCAGCTT
TCCAATGTTTTTTCATCTAAGCTTAGTCCAGAAGAACGTTTTAAACATGGCATCTGGACAGAACTTGAAACGGGTGCGCC
TGTACTCAATGATGCCCTGGTCAGTTTTGATTGTGAGATTGAGCAAATCCAGCAAGTGGGCACACATACCATTTTTATTT
GTCCGATTGTGGCGATTCAACAGAATCAACATCACCAGAGTCTGGTGTATTTTAATCGGGGCTATCACCATGTCGGACAT
AGTCTAGAACAGATTGAAGTGGCTTAA

Upstream 100 bases:

>100_bases
TGAGTCAATTGAGCTATGAATTGACTCAGATGATGTAATGACCTGATAGAATTGCGTAATTGGCCCCGAATGAAAAATCA
GGATTAAAGTAAGGAGCGAG

Downstream 100 bases:

>100_bases
TTTTATTTTGATTACATCTACAGACTATAGGTCTTAAGATAAGTTTCTAAGCATTTTGTTTGAGTGTTTCGAGCATATCT
GCGTCGGGATCATAAGTGGA

Product: flavoprotein oxidoreductase

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MMIETTDFRNAMSLLSSAVSVVTTAGLAGRYGFTASAVCSVTDTPPTLLVCMNQSSSSHMHFVENKILTVNVLSAQHQQL
SNVFSSKLSPEERFKHGIWTELETGAPVLNDALVSFDCEIEQIQQVGTHTIFICPIVAIQQNQHHQSLVYFNRGYHHVGH
SLEQIEVA

Sequences:

>Translated_168_residues
MMIETTDFRNAMSLLSSAVSVVTTAGLAGRYGFTASAVCSVTDTPPTLLVCMNQSSSSHMHFVENKILTVNVLSAQHQQL
SNVFSSKLSPEERFKHGIWTELETGAPVLNDALVSFDCEIEQIQQVGTHTIFICPIVAIQQNQHHQSLVYFNRGYHHVGH
SLEQIEVA
>Mature_168_residues
MMIETTDFRNAMSLLSSAVSVVTTAGLAGRYGFTASAVCSVTDTPPTLLVCMNQSSSSHMHFVENKILTVNVLSAQHQQL
SNVFSSKLSPEERFKHGIWTELETGAPVLNDALVSFDCEIEQIQQVGTHTIFICPIVAIQQNQHHQSLVYFNRGYHHVGH
SLEQIEVA

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=155, Percent_Identity=52.258064516129, Blast_Score=165, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 18529; Mature: 18529

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIETTDFRNAMSLLSSAVSVVTTAGLAGRYGFTASAVCSVTDTPPTLLVCMNQSSSSHM
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCEEEEEECCCCCCCE
HFVENKILTVNVLSAQHQQLSNVFSSKLSPEERFKHGIWTELETGAPVLNDALVSFDCEI
EEECCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCH
EQIQQVGTHTIFICPIVAIQQNQHHQSLVYFNRGYHHVGHSLEQIEVA
HHHHHCCCCEEEEEEEEEECCCCCCCEEEEEECCHHHHCCCHHHEECC
>Mature Secondary Structure
MMIETTDFRNAMSLLSSAVSVVTTAGLAGRYGFTASAVCSVTDTPPTLLVCMNQSSSSHM
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCEEEEEECCCCCCCE
HFVENKILTVNVLSAQHQQLSNVFSSKLSPEERFKHGIWTELETGAPVLNDALVSFDCEI
EEECCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCH
EQIQQVGTHTIFICPIVAIQQNQHHQSLVYFNRGYHHVGHSLEQIEVA
HHHHHCCCCEEEEEEEEEECCCCCCCEEEEEECCHHHHCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA