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The map label for this gene is ddl

Identifier: 50086472

GI number: 50086472

Start: 3442482

End: 3443411

Strand: Reverse

Name: ddl

Synonym: ACIAD3515

Alternate gene names: 50086472

Gene position: 3443411-3442482 (Counterclockwise)

Preceding gene: 50086473

Following gene: 50086471

Centisome position: 95.69

GC content: 47.1

Gene sequence:

>930_bases
GTGTCAAATGCTTCAAAATTCGGCAAAGTGGCGGTGTTGTTTGGTGGGAAGTCGGCTGAGCGGGACGTTTCTTTAGATAG
TGGTCAGGCCGTGCTTGATGCATTGTTACGTTCGGGTGTGCAGGCAGAAGCATTTGATCCAAAAGAACGTAGCGTGACAG
AGCTGGTTGGTTATGATCGTGCCTTTATTGTTTTGCATGGTCGTGGCGGGGAAGATGGTCAAATTCAGGGGGTACTTGAA
TGGCTGGATATTCCTTATACCGGTACAGGCGTACAGGGTTCTGCCATTGGAATGGATAAAATCAAGACCAAGCAGATTTG
GCAGGGGAGCGACCTTCCCACAGCGCCTTATCGCATTGTCAGCAAAGACTCAGACCTTAATGAGATTGTGGCTTCATTAG
GCTTGCCTGTGATTATTAAGCCAGTCCATGAGGGCTCGAGCATTGGCATGAGCAAAGTTGAAAAAATCGAAGACTTTGCA
CCAGCCATTGAAAAAGCGACAGCACACGATGCAATCGTGATGGCAGAAAAATGGATTACTGGTCGTGAATACACGATTGT
GGTGCTCAATGGAAAGGCATTGCCGGTCATTCGCTTACAACCACCGCAAGATGTTGCATTTTACGATTATGATGCCAAAT
ATCAGCGTAATGATGTGCAGTATGGTATTCCATCTGGTCTATCTGATAGCGATGAAAAGAAACTGCAAGAGCTCACATTG
CGTGCATTTCATACAGTTGGTGCAAGCGGCTGGGGCCGAGTCGACGCCATGCAAGATGAACACGGTAATTTCTGGTTGCT
TGAAATCAATACCGTACCTGGCATGACCAGTCACTCGCTCGTACCTAAAGCTGCACAAGCCATAGGTATCGATTTTGATG
CATTGTGCGTCGAAATTTTAGCGCAAACGCTTACAGGTTTGGCACACTAA

Upstream 100 bases:

>100_bases
TGGGAGCAATTTCCGTTGATCTTGCCCAGCAGCAATTGTATTTGAAATAAGTGTATTGCTTGATCATATTTAGTTTGCGA
TAAAATTTAAGGATAAAAAC

Downstream 100 bases:

>100_bases
ATATGGCTCAGCTTCCAGCATCAATGCGTCGTAAAAGAGCGGCGATTACCTCTATCCATGAAAAACCACCATCACGCAAG
CAAAAGCTTGTGAATGCAGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSNASKFGKVAVLFGGKSAERDVSLDSGQAVLDALLRSGVQAEAFDPKERSVTELVGYDRAFIVLHGRGGEDGQIQGVLE
WLDIPYTGTGVQGSAIGMDKIKTKQIWQGSDLPTAPYRIVSKDSDLNEIVASLGLPVIIKPVHEGSSIGMSKVEKIEDFA
PAIEKATAHDAIVMAEKWITGREYTIVVLNGKALPVIRLQPPQDVAFYDYDAKYQRNDVQYGIPSGLSDSDEKKLQELTL
RAFHTVGASGWGRVDAMQDEHGNFWLLEINTVPGMTSHSLVPKAAQAIGIDFDALCVEILAQTLTGLAH

Sequences:

>Translated_309_residues
MSNASKFGKVAVLFGGKSAERDVSLDSGQAVLDALLRSGVQAEAFDPKERSVTELVGYDRAFIVLHGRGGEDGQIQGVLE
WLDIPYTGTGVQGSAIGMDKIKTKQIWQGSDLPTAPYRIVSKDSDLNEIVASLGLPVIIKPVHEGSSIGMSKVEKIEDFA
PAIEKATAHDAIVMAEKWITGREYTIVVLNGKALPVIRLQPPQDVAFYDYDAKYQRNDVQYGIPSGLSDSDEKKLQELTL
RAFHTVGASGWGRVDAMQDEHGNFWLLEINTVPGMTSHSLVPKAAQAIGIDFDALCVEILAQTLTGLAH
>Mature_308_residues
SNASKFGKVAVLFGGKSAERDVSLDSGQAVLDALLRSGVQAEAFDPKERSVTELVGYDRAFIVLHGRGGEDGQIQGVLEW
LDIPYTGTGVQGSAIGMDKIKTKQIWQGSDLPTAPYRIVSKDSDLNEIVASLGLPVIIKPVHEGSSIGMSKVEKIEDFAP
AIEKATAHDAIVMAEKWITGREYTIVVLNGKALPVIRLQPPQDVAFYDYDAKYQRNDVQYGIPSGLSDSDEKKLQELTLR
AFHTVGASGWGRVDAMQDEHGNFWLLEINTVPGMTSHSLVPKAAQAIGIDFDALCVEILAQTLTGLAH

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=302, Percent_Identity=51.6556291390728, Blast_Score=303, Evalue=9e-84,
Organism=Escherichia coli, GI1786579, Length=349, Percent_Identity=30.3724928366762, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_ACIAD (Q6F705)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047982.1
- HSSP:   P07862
- ProteinModelPortal:   Q6F705
- SMR:   Q6F705
- STRING:   Q6F705
- GeneID:   2881096
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3515
- NMPDR:   fig|62977.3.peg.3175
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   WGRVDAM
- PhylomeDB:   Q6F705
- ProtClustDB:   PRK01372
- BioCyc:   ASP62977:ACIAD3515-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33434; Mature: 33303

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNASKFGKVAVLFGGKSAERDVSLDSGQAVLDALLRSGVQAEAFDPKERSVTELVGYDR
CCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCE
AFIVLHGRGGEDGQIQGVLEWLDIPYTGTGVQGSAIGMDKIKTKQIWQGSDLPTAPYRIV
EEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHHHCCCCCCCCCCEEEE
SKDSDLNEIVASLGLPVIIKPVHEGSSIGMSKVEKIEDFAPAIEKATAHDAIVMAEKWIT
ECCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCC
GREYTIVVLNGKALPVIRLQPPQDVAFYDYDAKYQRNDVQYGIPSGLSDSDEKKLQELTL
CCCEEEEEECCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
RAFHTVGASGWGRVDAMQDEHGNFWLLEINTVPGMTSHSLVPKAAQAIGIDFDALCVEIL
HHHHHCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHH
AQTLTGLAH
HHHHHHCCC
>Mature Secondary Structure 
SNASKFGKVAVLFGGKSAERDVSLDSGQAVLDALLRSGVQAEAFDPKERSVTELVGYDR
CCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCE
AFIVLHGRGGEDGQIQGVLEWLDIPYTGTGVQGSAIGMDKIKTKQIWQGSDLPTAPYRIV
EEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHHHCCCCCCCCCCEEEE
SKDSDLNEIVASLGLPVIIKPVHEGSSIGMSKVEKIEDFAPAIEKATAHDAIVMAEKWIT
ECCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCC
GREYTIVVLNGKALPVIRLQPPQDVAFYDYDAKYQRNDVQYGIPSGLSDSDEKKLQELTL
CCCEEEEEECCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
RAFHTVGASGWGRVDAMQDEHGNFWLLEINTVPGMTSHSLVPKAAQAIGIDFDALCVEIL
HHHHHCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHH
AQTLTGLAH
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA