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The map label for this gene is xenB [H]

Identifier: 50086454

GI number: 50086454

Start: 3420162

End: 3421232

Strand: Reverse

Name: xenB [H]

Synonym: ACIAD3496

Alternate gene names: 50086454

Gene position: 3421232-3420162 (Counterclockwise)

Preceding gene: 50086455

Following gene: 50086453

Centisome position: 95.07

GC content: 45.38

Gene sequence:

>1071_bases
ATGGCTGAATTAAGTTCTCCTTTGAAAATAGGCGCTTTTGAAATTAAAAATCGTCTTGTGATGGCGCCACTAACACGTTC
TCGTAGTGGTGAGGATCGGATTCCAAATGATTTAATGTTGGAATATTACCAGCAAAGAGCTGGTGCAGGTCTGATTTTGA
CTGAAGCGACTGTGATTTCACCTAAAGCGGTTGGTTATGAAGATACCCCAGGCTTATGGTCGGAAGAACAGGCAAGCGCG
TGGCATCCGATTGTTGATGCCGTACATCAAAAAGGTGCCAAAATCGTGGTTCAGCTGTGGCATGTAGGGCGCATTTCCGA
TCCTCAACTTTTGGGTGGTGATATACCTGTTGCACCAAGTGCGATCCAGCCAGAGGGACACGTCAGCCTACTACGTCCAA
AGCGTCCTTTTGTAACGCCACGTGCTTTATCGATTGAAGAAATTCATGAAATTGTGGCGCAATATCAGCATGCTGCAGAG
TTGGCAAAAGTGGCAGGCTTTGATGGTGTAGAGCTACATGCAGCAAATGGTTATTTAATTGATCAGTTTTTACAGTCGGG
TACCAATCAGCGTAGTGATGAATACGGTGGCTCACTTGAAAATCGAGCACGATTACTATTACAGGTGGTCGATGCATTTA
TTGACGTCTGGGGTGCAGGGCGTGTTGGTGTACATATAGCGCCTCGCGGTGACTCACACGATATGAGTGATGCCAATCCG
CTCGAAACCTTTGGTTATGTCATGCGTCAGCTCAGTGAACGTCACATTGCATTTGTATTCTCGCGTGAATATGAAGCTGC
GGATAGTATTAGCCTACAACTACGTCAAAAATTTAATGGCGTATGGATTGCCAATGAAAATCTGAGCGCTGAATCTGCCA
AACGTATTTTGGCACAGGGTGATGCCGATGCTGTCTCATTTGGGAAGGCTGCAATTGCCAATCCTGATTTGTATGAGCGT
CTGATTCATAATGCACCACTCAATAGCGTAAATCCTGACACACTTTATGCCAAAGGTGCAGAAGGTTATACCGATTATCC
AACTTCACAAGAAAGTGCTCTCGAAGCCTAA

Upstream 100 bases:

>100_bases
ATATGGGCAATGGTCATATTTTTCACGTAATGAAGCATTGATTCAGCAATATCTTAATCATTTAAAAGAAACGCTATAAC
ACGCGTAAGCTGAGGCGATA

Downstream 100 bases:

>100_bases
GACTAAAAATCTAATTGAAGGCAGCATATATTTGTAACTAAGAAATATTTTATAATCTATGCTGCTTTTTACCATCACTA
CACAATTATAATGAATGTGA

Product: xenobiotic reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MAELSSPLKIGAFEIKNRLVMAPLTRSRSGEDRIPNDLMLEYYQQRAGAGLILTEATVISPKAVGYEDTPGLWSEEQASA
WHPIVDAVHQKGAKIVVQLWHVGRISDPQLLGGDIPVAPSAIQPEGHVSLLRPKRPFVTPRALSIEEIHEIVAQYQHAAE
LAKVAGFDGVELHAANGYLIDQFLQSGTNQRSDEYGGSLENRARLLLQVVDAFIDVWGAGRVGVHIAPRGDSHDMSDANP
LETFGYVMRQLSERHIAFVFSREYEAADSISLQLRQKFNGVWIANENLSAESAKRILAQGDADAVSFGKAAIANPDLYER
LIHNAPLNSVNPDTLYAKGAEGYTDYPTSQESALEA

Sequences:

>Translated_356_residues
MAELSSPLKIGAFEIKNRLVMAPLTRSRSGEDRIPNDLMLEYYQQRAGAGLILTEATVISPKAVGYEDTPGLWSEEQASA
WHPIVDAVHQKGAKIVVQLWHVGRISDPQLLGGDIPVAPSAIQPEGHVSLLRPKRPFVTPRALSIEEIHEIVAQYQHAAE
LAKVAGFDGVELHAANGYLIDQFLQSGTNQRSDEYGGSLENRARLLLQVVDAFIDVWGAGRVGVHIAPRGDSHDMSDANP
LETFGYVMRQLSERHIAFVFSREYEAADSISLQLRQKFNGVWIANENLSAESAKRILAQGDADAVSFGKAAIANPDLYER
LIHNAPLNSVNPDTLYAKGAEGYTDYPTSQESALEA
>Mature_355_residues
AELSSPLKIGAFEIKNRLVMAPLTRSRSGEDRIPNDLMLEYYQQRAGAGLILTEATVISPKAVGYEDTPGLWSEEQASAW
HPIVDAVHQKGAKIVVQLWHVGRISDPQLLGGDIPVAPSAIQPEGHVSLLRPKRPFVTPRALSIEEIHEIVAQYQHAAEL
AKVAGFDGVELHAANGYLIDQFLQSGTNQRSDEYGGSLENRARLLLQVVDAFIDVWGAGRVGVHIAPRGDSHDMSDANPL
ETFGYVMRQLSERHIAFVFSREYEAADSISLQLRQKFNGVWIANENLSAESAKRILAQGDADAVSFGKAAIANPDLYERL
IHNAPLNSVNPDTLYAKGAEGYTDYPTSQESALEA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=361, Percent_Identity=46.5373961218837, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1789463, Length=214, Percent_Identity=37.8504672897196, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17559804, Length=232, Percent_Identity=33.6206896551724, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI72001454, Length=342, Percent_Identity=27.4853801169591, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17559802, Length=220, Percent_Identity=33.1818181818182, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=235, Percent_Identity=31.4893617021277, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=343, Percent_Identity=25.6559766763848, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=185, Percent_Identity=29.7297297297297, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=215, Percent_Identity=30.2325581395349, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321973, Length=380, Percent_Identity=36.5789473684211, Blast_Score=212, Evalue=9e-56,
Organism=Saccharomyces cerevisiae, GI6325086, Length=377, Percent_Identity=36.604774535809, Blast_Score=207, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38959; Mature: 38828

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELSSPLKIGAFEIKNRLVMAPLTRSRSGEDRIPNDLMLEYYQQRAGAGLILTEATVIS
CCCCCCCCEECHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEC
PKAVGYEDTPGLWSEEQASAWHPIVDAVHQKGAKIVVQLWHVGRISDPQLLGGDIPVAPS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCEECCCCCCCCC
AIQPEGHVSLLRPKRPFVTPRALSIEEIHEIVAQYQHAAELAKVAGFDGVELHAANGYLI
CCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
DQFLQSGTNQRSDEYGGSLENRARLLLQVVDAFIDVWGAGRVGVHIAPRGDSHDMSDANP
HHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCH
LETFGYVMRQLSERHIAFVFSREYEAADSISLQLRQKFNGVWIANENLSAESAKRILAQG
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCC
DADAVSFGKAAIANPDLYERLIHNAPLNSVNPDTLYAKGAEGYTDYPTSQESALEA
CCCHHHHCCHHCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure 
AELSSPLKIGAFEIKNRLVMAPLTRSRSGEDRIPNDLMLEYYQQRAGAGLILTEATVIS
CCCCCCCEECHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEC
PKAVGYEDTPGLWSEEQASAWHPIVDAVHQKGAKIVVQLWHVGRISDPQLLGGDIPVAPS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCEECCCCCCCCC
AIQPEGHVSLLRPKRPFVTPRALSIEEIHEIVAQYQHAAELAKVAGFDGVELHAANGYLI
CCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
DQFLQSGTNQRSDEYGGSLENRARLLLQVVDAFIDVWGAGRVGVHIAPRGDSHDMSDANP
HHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCH
LETFGYVMRQLSERHIAFVFSREYEAADSISLQLRQKFNGVWIANENLSAESAKRILAQG
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCC
DADAVSFGKAAIANPDLYERLIHNAPLNSVNPDTLYAKGAEGYTDYPTSQESALEA
CCCHHHHCCHHCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]