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The map label for this gene is hisJ [H]

Identifier: 50086448

GI number: 50086448

Start: 3413513

End: 3414358

Strand: Reverse

Name: hisJ [H]

Synonym: ACIAD3487

Alternate gene names: 50086448

Gene position: 3414358-3413513 (Counterclockwise)

Preceding gene: 50086449

Following gene: 50086447

Centisome position: 94.88

GC content: 40.54

Gene sequence:

>846_bases
ATGAAAATGAAAGGGGCATTTACAGTAAGTTTGCTGGTAAGTAGCTTGATGCTGACTGCGTGTTCTAAAGGCACAGAGCA
AAGCCATACGACGACGCAAGACACTGCCAAAAAAACAACATTAAACGTAGGCTCGGATATGACCTTTCCGCCTTATGAAT
ATTTGGAGGATGGAAAACCTAAAGGCGTTGATGTCGAAATTATGGCAAAAATAGCAGAAAGCGATGGCTCCTATCAACCG
AACTGGATCGATACCCGTTGGGCGAATCTCATCCCTGGTCTGAAAGGTAAAAAGTTTGATGTGCTATTTTCTTCGATGTA
CATCACCAAAGAGCGTTTACAACAAATTGACATGATCCCCTATTATAAAACTGACATTGCATTGTTGGTTCGTGGTGATT
CTACTTTAAATCCAAAAGGGCCTGATGATTTATGTGGAACTGTGGTTGGAACGATGAAAGGGACTGCTTTTGCAACACAA
CTACAGGAGATTTCGGAAAAACGTTGCGTGGCTAAAGGTAAAGCAGAAATTAAGGTCAGAGAATTTGAAAGCTCACCTCA
AACCACACAATCTTTACTGGCTCATGCTGTTGATATTCAGTATGACGATGCAGCAGTAATGGCGAATGCGGTAAAAAATC
TAGGCAGTAAAGTTAAAGTGACCTCAACTGAATCATTCTATCCAATTTTAGGTGGAATTGGTGTTCGCAAAGGTGACACT
GAAACCTATAAAAAAATCGAAGATGGTTTAAATAAAATCAAGGCTTCTGGTGAGTATCACAAGATTCTGGCGGCACATGG
CTTACAAGAGCCTACGGCAGATGATATTGCGAAATTAATGAAATAA

Upstream 100 bases:

>100_bases
TAAGATTTTATTAAGAACTAGATCAGTAAATACAAATTATCAAACGCGAAAAATCACCTATATTGGATCTTAGGCGATTA
CATAACTCAGGTGAAAAGAG

Downstream 100 bases:

>100_bases
CATCTGATACGAAGAGGATAAATTCAATGCTAACTCAAGAACAAATCCAGCAATTTCATCAGAATGGTTATCTGGTCGTA
GAAAATGTAATCCAGCAGGA

Product: amino acid ABC transporter periplasmic substrate-binding protein

Products: NA

Alternate protein names: HBP [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKMKGAFTVSLLVSSLMLTACSKGTEQSHTTTQDTAKKTTLNVGSDMTFPPYEYLEDGKPKGVDVEIMAKIAESDGSYQP
NWIDTRWANLIPGLKGKKFDVLFSSMYITKERLQQIDMIPYYKTDIALLVRGDSTLNPKGPDDLCGTVVGTMKGTAFATQ
LQEISEKRCVAKGKAEIKVREFESSPQTTQSLLAHAVDIQYDDAAVMANAVKNLGSKVKVTSTESFYPILGGIGVRKGDT
ETYKKIEDGLNKIKASGEYHKILAAHGLQEPTADDIAKLMK

Sequences:

>Translated_281_residues
MKMKGAFTVSLLVSSLMLTACSKGTEQSHTTTQDTAKKTTLNVGSDMTFPPYEYLEDGKPKGVDVEIMAKIAESDGSYQP
NWIDTRWANLIPGLKGKKFDVLFSSMYITKERLQQIDMIPYYKTDIALLVRGDSTLNPKGPDDLCGTVVGTMKGTAFATQ
LQEISEKRCVAKGKAEIKVREFESSPQTTQSLLAHAVDIQYDDAAVMANAVKNLGSKVKVTSTESFYPILGGIGVRKGDT
ETYKKIEDGLNKIKASGEYHKILAAHGLQEPTADDIAKLMK
>Mature_281_residues
MKMKGAFTVSLLVSSLMLTACSKGTEQSHTTTQDTAKKTTLNVGSDMTFPPYEYLEDGKPKGVDVEIMAKIAESDGSYQP
NWIDTRWANLIPGLKGKKFDVLFSSMYITKERLQQIDMIPYYKTDIALLVRGDSTLNPKGPDDLCGTVVGTMKGTAFATQ
LQEISEKRCVAKGKAEIKVREFESSPQTTQSLLAHAVDIQYDDAAVMANAVKNLGSKVKVTSTESFYPILGGIGVRKGDT
ETYKKIEDGLNKIKASGEYHKILAAHGLQEPTADDIAKLMK

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=226, Percent_Identity=27.4336283185841, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787085, Length=230, Percent_Identity=25.6521739130435, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=24.6753246753247, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787031, Length=274, Percent_Identity=25.1824817518248, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787088, Length=233, Percent_Identity=25.7510729613734, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30747; Mature: 30747

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKGAFTVSLLVSSLMLTACSKGTEQSHTTTQDTAKKTTLNVGSDMTFPPYEYLEDGKP
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEECCCCCCCCCHHHHHCCCC
KGVDVEIMAKIAESDGSYQPNWIDTRWANLIPGLKGKKFDVLFSSMYITKERLQQIDMIP
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
YYKTDIALLVRGDSTLNPKGPDDLCGTVVGTMKGTAFATQLQEISEKRCVAKGKAEIKVR
EECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEE
EFESSPQTTQSLLAHAVDIQYDDAAVMANAVKNLGSKVKVTSTESFYPILGGIGVRKGDT
ECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCCCH
ETYKKIEDGLNKIKASGEYHKILAAHGLQEPTADDIAKLMK
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHC
>Mature Secondary Structure
MKMKGAFTVSLLVSSLMLTACSKGTEQSHTTTQDTAKKTTLNVGSDMTFPPYEYLEDGKP
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEECCCCCCCCCHHHHHCCCC
KGVDVEIMAKIAESDGSYQPNWIDTRWANLIPGLKGKKFDVLFSSMYITKERLQQIDMIP
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
YYKTDIALLVRGDSTLNPKGPDDLCGTVVGTMKGTAFATQLQEISEKRCVAKGKAEIKVR
EECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEE
EFESSPQTTQSLLAHAVDIQYDDAAVMANAVKNLGSKVKVTSTESFYPILGGIGVRKGDT
ECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCCCH
ETYKKIEDGLNKIKASGEYHKILAAHGLQEPTADDIAKLMK
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]