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The map label for this gene is kdtA [H]

Identifier: 50086440

GI number: 50086440

Start: 3404923

End: 3406206

Strand: Reverse

Name: kdtA [H]

Synonym: ACIAD3479

Alternate gene names: 50086440

Gene position: 3406206-3404923 (Counterclockwise)

Preceding gene: 50086442

Following gene: 50086439

Centisome position: 94.65

GC content: 43.15

Gene sequence:

>1284_bases
TTGGCGACTCCATTTTGGTATAACGCTGCATTAAAAGTGTTAAAGCCTGTTTATCGCTGGCGGATTAAAAAGCGGGCTGA
AAATGAAATGCGTTACCAGCAGGAATGTCGTGAGCGCTTTGGTCCGTTTCAGCAGCCCCAAAATCGTCATGCATTATGGT
TTCATGCGGTTTCGGTGGGTGAAACCAATGCAGCTCAGCCTTTAATAGAATATTATCTAAAACTGGGCCATCCTGTGTTG
GTGACGAACACCACAAAAACAGGGCAAGCACGTGCCAAGTCATTATTTTTAAAATCTCCTTATCTGGAGCTGTTTCAGGC
AGTGTATTTACCTGTCGATCAGATATTTTTACTCAAACAATTTTTTGAGCTGTATCAACCCAAACTACTGGCGTTAGTTG
AAACCGAGCTTTGGCCCAATCTGATTGATCAGGCACAACATTACGGCGTTCCCACATTTTTAATCAATGCACGACTATCA
GAAAAATCGGCACAGGGCTATGCACGGGTATCTGCTCTAAGTAATGCAATGCTGAGTCGCTTAACTGGTTTACTGGCACA
AGATCAATCCACTCAGCAGCGTTTTATTGGGCTTGGACTAGCAAGAGAAAAAGCTCAAGTGGTGGGTAATATCAAATTTG
ATATAGACGTGCCTTTGCTGTTTGTTGAACAAGCCAGAAGATTGCAACAACAATGGCAACTGGCTGGTCGTAAAATCATG
GTTTTGGCCAGTACACATGCACCTGAAGAGCAACAACTTTTAACTGCACTGAAAACACATTTAATGGCAGATCCAGATTT
GTTATGTATTGTGGTTCCACGTCATCCAGAACGTTTTGATGAGGTTTTTCAGATTTGTCAAAATCTACAGCTTGATACAG
CTCGGCGTAGTTTGGAGCAACCGATACAGCCAGAAACTCAAGTCTATCTGGCAGATAGTATGGGCGAATTGTGGTTGTGG
TATGCGCTGGCACAGACTGCATTTGTTGGCGGTTCGCTCAATGAACCTGGTGGTGGCCATAATATTCTTGAACCCATGGC
ACTTAAGATTCCAGTGGTGATTGGACCGCGCTATTTTAATTTTCAGACTATTGTTGATGAATTTAGAGAAACCGAGGCGA
TTGATATCGTGCAGGATGCGACTCAGGCGGTGCGGCAATTATTACATTATGTAAGCACTCCAAACAGTGCTGATCAGATA
GTCGATCATGCGCAGTCCATTTTGCAACGTAACAAAGGCTCGTTAGCACGACATATTCATGTATTGGATCAATATTTATC
ATGA

Upstream 100 bases:

>100_bases
ATGTTAAAATGAGCAGCATACATTTTGAAATACACTACATTAACAAATCTATGGCGCTCTGCTATATTCTGCCCATTCTA
ATTAAATTGAGAAATAGATA

Downstream 100 bases:

>100_bases
ATATTGTATTGCTTGAACCCAAAGATACCGCGCAAGCTGAATGGCGCATTCATTCAGCACGTCAGCTGAGTCATCTACAT
AAACAACTCAATATTCAAGT

Product: 3-deoxy-D-manno-2-octulosonate transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MATPFWYNAALKVLKPVYRWRIKKRAENEMRYQQECRERFGPFQQPQNRHALWFHAVSVGETNAAQPLIEYYLKLGHPVL
VTNTTKTGQARAKSLFLKSPYLELFQAVYLPVDQIFLLKQFFELYQPKLLALVETELWPNLIDQAQHYGVPTFLINARLS
EKSAQGYARVSALSNAMLSRLTGLLAQDQSTQQRFIGLGLAREKAQVVGNIKFDIDVPLLFVEQARRLQQQWQLAGRKIM
VLASTHAPEEQQLLTALKTHLMADPDLLCIVVPRHPERFDEVFQICQNLQLDTARRSLEQPIQPETQVYLADSMGELWLW
YALAQTAFVGGSLNEPGGGHNILEPMALKIPVVIGPRYFNFQTIVDEFRETEAIDIVQDATQAVRQLLHYVSTPNSADQI
VDHAQSILQRNKGSLARHIHVLDQYLS

Sequences:

>Translated_427_residues
MATPFWYNAALKVLKPVYRWRIKKRAENEMRYQQECRERFGPFQQPQNRHALWFHAVSVGETNAAQPLIEYYLKLGHPVL
VTNTTKTGQARAKSLFLKSPYLELFQAVYLPVDQIFLLKQFFELYQPKLLALVETELWPNLIDQAQHYGVPTFLINARLS
EKSAQGYARVSALSNAMLSRLTGLLAQDQSTQQRFIGLGLAREKAQVVGNIKFDIDVPLLFVEQARRLQQQWQLAGRKIM
VLASTHAPEEQQLLTALKTHLMADPDLLCIVVPRHPERFDEVFQICQNLQLDTARRSLEQPIQPETQVYLADSMGELWLW
YALAQTAFVGGSLNEPGGGHNILEPMALKIPVVIGPRYFNFQTIVDEFRETEAIDIVQDATQAVRQLLHYVSTPNSADQI
VDHAQSILQRNKGSLARHIHVLDQYLS
>Mature_426_residues
ATPFWYNAALKVLKPVYRWRIKKRAENEMRYQQECRERFGPFQQPQNRHALWFHAVSVGETNAAQPLIEYYLKLGHPVLV
TNTTKTGQARAKSLFLKSPYLELFQAVYLPVDQIFLLKQFFELYQPKLLALVETELWPNLIDQAQHYGVPTFLINARLSE
KSAQGYARVSALSNAMLSRLTGLLAQDQSTQQRFIGLGLAREKAQVVGNIKFDIDVPLLFVEQARRLQQQWQLAGRKIMV
LASTHAPEEQQLLTALKTHLMADPDLLCIVVPRHPERFDEVFQICQNLQLDTARRSLEQPIQPETQVYLADSMGELWLWY
ALAQTAFVGGSLNEPGGGHNILEPMALKIPVVIGPRYFNFQTIVDEFRETEAIDIVQDATQAVRQLLHYVSTPNSADQIV
DHAQSILQRNKGSLARHIHVLDQYLS

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=425, Percent_Identity=36.9411764705882, Blast_Score=258, Evalue=6e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 48825; Mature: 48694

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPFWYNAALKVLKPVYRWRIKKRAENEMRYQQECRERFGPFQQPQNRHALWFHAVSVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECC
ETNAAQPLIEYYLKLGHPVLVTNTTKTGQARAKSLFLKSPYLELFQAVYLPVDQIFLLKQ
CCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHH
FFELYQPKLLALVETELWPNLIDQAQHYGVPTFLINARLSEKSAQGYARVSALSNAMLSR
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCHHHHHHHHHHHHHHHHHHHH
LTGLLAQDQSTQQRFIGLGLAREKAQVVGNIKFDIDVPLLFVEQARRLQQQWQLAGRKIM
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEE
VLASTHAPEEQQLLTALKTHLMADPDLLCIVVPRHPERFDEVFQICQNLQLDTARRSLEQ
EEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHC
PIQPETQVYLADSMGELWLWYALAQTAFVGGSLNEPGGGHNILEPMALKIPVVIGPRYFN
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEEEEECCCCCC
FQTIVDEFRETEAIDIVQDATQAVRQLLHYVSTPNSADQIVDHAQSILQRNKGSLARHIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
VLDQYLS
HHHHHHC
>Mature Secondary Structure 
ATPFWYNAALKVLKPVYRWRIKKRAENEMRYQQECRERFGPFQQPQNRHALWFHAVSVG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECC
ETNAAQPLIEYYLKLGHPVLVTNTTKTGQARAKSLFLKSPYLELFQAVYLPVDQIFLLKQ
CCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHH
FFELYQPKLLALVETELWPNLIDQAQHYGVPTFLINARLSEKSAQGYARVSALSNAMLSR
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCHHHHHHHHHHHHHHHHHHHH
LTGLLAQDQSTQQRFIGLGLAREKAQVVGNIKFDIDVPLLFVEQARRLQQQWQLAGRKIM
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEE
VLASTHAPEEQQLLTALKTHLMADPDLLCIVVPRHPERFDEVFQICQNLQLDTARRSLEQ
EEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHC
PIQPETQVYLADSMGELWLWYALAQTAFVGGSLNEPGGGHNILEPMALKIPVVIGPRYFN
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEEEEECCCCCC
FQTIVDEFRETEAIDIVQDATQAVRQLLHYVSTPNSADQIVDHAQSILQRNKGSLARHIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
VLDQYLS
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]