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The map label for this gene is oprM [H]

Identifier: 50086389

GI number: 50086389

Start: 3330230

End: 3331675

Strand: Reverse

Name: oprM [H]

Synonym: ACIAD3421

Alternate gene names: 50086389

Gene position: 3331675-3330230 (Counterclockwise)

Preceding gene: 50086390

Following gene: 50086386

Centisome position: 92.58

GC content: 43.64

Gene sequence:

>1446_bases
ATGCATCAGTTTTTCTCATTACAGATCCGAGTTAAACCTTCTAAGTTGCTCAGTGCCTTTTCTTTAGGTGTCATGGGGGT
ATTGTCAGGTTGTCAAATAGTGAAACCAGAACCCAGTGCGCAACTGAATATACAGGCACAATTCTCTCAACCTGCTCAAT
ATGGATTAGATGCCAAAAAATGGGAAACTGAAGATTGGAAAATTGCTGTGCCAAGTGATCAGCTGCCAAGAGGAGAATGG
TGGCAAATTTTTCAGGATACCCAGTTAAATCAATTGGTCGAACAATTGAATCGTGAAAATGCTTCAATTGCTCAATATGA
AGCCCAATACCGTCAAGCTACAGCATTACTCAATCAAGCTACCGCGAGTACCAAACCAACTGTGACAGGAAATACGAATG
TTACGCGGGCAAAATCTGGTGAGAGTGCTACAACCGCAAACTATAACATGGGACTAAATGCCAGTTGGGAACCTGATTTT
TGGGGCAAAGTCCGCCAAAATATATGGGTGAATCAGGATAAAGCCCAAGCAAGTGAAGCCGAACTCAATTCGATTCGCCT
GAGTATGCAGGCACAATTAACCACCAGCTATCTACAATGGGTGATTCTGGGATTTCAGCTACAATCACAGCAAGAGAGCC
TGAAAAACCTGCAAAAATTTTTGCAGCTCACACACAATCAATATCAAGCAGGGATTGTGGCATCAACGACAGTTGATCAG
GCACAAAGCCAGTATCAAACTGCGCTCGCAAATTATACCGAAATGCAATTGGCACAAATACAGTTACAGCATGCGATTGC
GATGTTAATTGGACAGCCTCAAGATCAGTTGCAACTGATCCTGCCGAAAACATTACCTGTGCTGCCACAAATCCCTGTAA
ATTTACCTTCAAGTATTTTGCAGCGCCGTCCAGATGTGGCTGCCGCAGAAAGAACGATGGCCGCAGCAAATGCACAGGTC
GGTGTAGCAAAATTGGCAGTTTTCCCAGATTTCAGTCTAGGCGGAAATCTCGGTTATAAAAGTAGTAAATTAAGTAATTT
ATTCAGTGCACCTAACTTTATCTGGTCTGTTGGGCCTACACTGGCAGCGACTTTATTTGATGGTGGACTGCGTCATGCAC
AAACAGAACAGGCCAAGGCAAGCTACGATGCAAGCGCCGCCGTGTATAAACAAACAGTGATGTCAGCGATTCAAAATGTT
GAAGATAACATCGCTGCACAATATCTACTCTCGAAGGAAGTTGCACAGGAGCAACAGAGTTTAGCTGCTGCTACACGTGC
CGAGCAGAGCACAACAAACCAATATAAAGCAGGTATTGTTGGGTATTTAAATGTACTCACTGCCCAGAATAACCGCCTAT
CTGCTCAAAACAATGTATGGTCATTGACGAGTAAACAATATCAGACCACCGTCAATTTGATCAGCGCTATGGGCGGAAGT
TTTTAA

Upstream 100 bases:

>100_bases
TTTATATATCGATCAGTTCAGTCGATGGGGAAGACGCAAGCTGCAACATATCTTCCCAGCCAAATCATTAAGTTTGAAAA
AATAAGAAAAGGACAATCAT

Downstream 100 bases:

>100_bases
GTGAAATATGATTGATCTGGTTTTTGATTTACAGGAAGATTTTTAGTGATTTAGTGATTTAGTGATTTAGTGATTTAGTG
ATTTAGTGATTTAGTGATTT

Product: outer membrane protein precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MHQFFSLQIRVKPSKLLSAFSLGVMGVLSGCQIVKPEPSAQLNIQAQFSQPAQYGLDAKKWETEDWKIAVPSDQLPRGEW
WQIFQDTQLNQLVEQLNRENASIAQYEAQYRQATALLNQATASTKPTVTGNTNVTRAKSGESATTANYNMGLNASWEPDF
WGKVRQNIWVNQDKAQASEAELNSIRLSMQAQLTTSYLQWVILGFQLQSQQESLKNLQKFLQLTHNQYQAGIVASTTVDQ
AQSQYQTALANYTEMQLAQIQLQHAIAMLIGQPQDQLQLILPKTLPVLPQIPVNLPSSILQRRPDVAAAERTMAAANAQV
GVAKLAVFPDFSLGGNLGYKSSKLSNLFSAPNFIWSVGPTLAATLFDGGLRHAQTEQAKASYDASAAVYKQTVMSAIQNV
EDNIAAQYLLSKEVAQEQQSLAAATRAEQSTTNQYKAGIVGYLNVLTAQNNRLSAQNNVWSLTSKQYQTTVNLISAMGGS
F

Sequences:

>Translated_481_residues
MHQFFSLQIRVKPSKLLSAFSLGVMGVLSGCQIVKPEPSAQLNIQAQFSQPAQYGLDAKKWETEDWKIAVPSDQLPRGEW
WQIFQDTQLNQLVEQLNRENASIAQYEAQYRQATALLNQATASTKPTVTGNTNVTRAKSGESATTANYNMGLNASWEPDF
WGKVRQNIWVNQDKAQASEAELNSIRLSMQAQLTTSYLQWVILGFQLQSQQESLKNLQKFLQLTHNQYQAGIVASTTVDQ
AQSQYQTALANYTEMQLAQIQLQHAIAMLIGQPQDQLQLILPKTLPVLPQIPVNLPSSILQRRPDVAAAERTMAAANAQV
GVAKLAVFPDFSLGGNLGYKSSKLSNLFSAPNFIWSVGPTLAATLFDGGLRHAQTEQAKASYDASAAVYKQTVMSAIQNV
EDNIAAQYLLSKEVAQEQQSLAAATRAEQSTTNQYKAGIVGYLNVLTAQNNRLSAQNNVWSLTSKQYQTTVNLISAMGGS
F
>Mature_481_residues
MHQFFSLQIRVKPSKLLSAFSLGVMGVLSGCQIVKPEPSAQLNIQAQFSQPAQYGLDAKKWETEDWKIAVPSDQLPRGEW
WQIFQDTQLNQLVEQLNRENASIAQYEAQYRQATALLNQATASTKPTVTGNTNVTRAKSGESATTANYNMGLNASWEPDF
WGKVRQNIWVNQDKAQASEAELNSIRLSMQAQLTTSYLQWVILGFQLQSQQESLKNLQKFLQLTHNQYQAGIVASTTVDQ
AQSQYQTALANYTEMQLAQIQLQHAIAMLIGQPQDQLQLILPKTLPVLPQIPVNLPSSILQRRPDVAAAERTMAAANAQV
GVAKLAVFPDFSLGGNLGYKSSKLSNLFSAPNFIWSVGPTLAATLFDGGLRHAQTEQAKASYDASAAVYKQTVMSAIQNV
EDNIAAQYLLSKEVAQEQQSLAAATRAEQSTTNQYKAGIVGYLNVLTAQNNRLSAQNNVWSLTSKQYQTTVNLISAMGGS
F

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=481, Percent_Identity=25.5717255717256, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI2367348, Length=509, Percent_Identity=22.7897838899804, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52926; Mature: 52926

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQFFSLQIRVKPSKLLSAFSLGVMGVLSGCQIVKPEPSAQLNIQAQFSQPAQYGLDAKK
CCCEEEEEEEECHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCHHHCCCCCC
WETEDWKIAVPSDQLPRGEWWQIFQDTQLNQLVEQLNRENASIAQYEAQYRQATALLNQA
CCCCCCEEECCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
TASTKPTVTGNTNVTRAKSGESATTANYNMGLNASWEPDFWGKVRQNIWVNQDKAQASEA
CCCCCCEECCCCCCEECCCCCCCEEECCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHH
ELNSIRLSMQAQLTTSYLQWVILGFQLQSQQESLKNLQKFLQLTHNQYQAGIVASTTVDQ
HHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCEEEECHHHH
AQSQYQTALANYTEMQLAQIQLQHAIAMLIGQPQDQLQLILPKTLPVLPQIPVNLPSSIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCCCCCCCCCCCCCHHHH
QRRPDVAAAERTMAAANAQVGVAKLAVFPDFSLGGNLGYKSSKLSNLFSAPNFIWSVGPT
HCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHEECCHH
LAATLFDGGLRHAQTEQAKASYDASAAVYKQTVMSAIQNVEDNIAAQYLLSKEVAQEQQS
HHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAATRAEQSTTNQYKAGIVGYLNVLTAQNNRLSAQNNVWSLTSKQYQTTVNLISAMGGS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCC
F
C
>Mature Secondary Structure
MHQFFSLQIRVKPSKLLSAFSLGVMGVLSGCQIVKPEPSAQLNIQAQFSQPAQYGLDAKK
CCCEEEEEEEECHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCHHHCCCCCC
WETEDWKIAVPSDQLPRGEWWQIFQDTQLNQLVEQLNRENASIAQYEAQYRQATALLNQA
CCCCCCEEECCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
TASTKPTVTGNTNVTRAKSGESATTANYNMGLNASWEPDFWGKVRQNIWVNQDKAQASEA
CCCCCCEECCCCCCEECCCCCCCEEECCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHH
ELNSIRLSMQAQLTTSYLQWVILGFQLQSQQESLKNLQKFLQLTHNQYQAGIVASTTVDQ
HHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCEEEECHHHH
AQSQYQTALANYTEMQLAQIQLQHAIAMLIGQPQDQLQLILPKTLPVLPQIPVNLPSSIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCCCCCCCCCCCCCHHHH
QRRPDVAAAERTMAAANAQVGVAKLAVFPDFSLGGNLGYKSSKLSNLFSAPNFIWSVGPT
HCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHEECCHH
LAATLFDGGLRHAQTEQAKASYDASAAVYKQTVMSAIQNVEDNIAAQYLLSKEVAQEQQS
HHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAATRAEQSTTNQYKAGIVGYLNVLTAQNNRLSAQNNVWSLTSKQYQTTVNLISAMGGS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]