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The map label for this gene is nucA [H]

Identifier: 50086382

GI number: 50086382

Start: 3323651

End: 3324727

Strand: Reverse

Name: nucA [H]

Synonym: ACIAD3408

Alternate gene names: 50086382

Gene position: 3324727-3323651 (Counterclockwise)

Preceding gene: 50086384

Following gene: 50086381

Centisome position: 92.39

GC content: 45.5

Gene sequence:

>1077_bases
GTGGCACGTAAACGAAGTCAGACACAGTCAAATTCATCTTGGTGGAATCGCTATATGGGTAAATTCATACTGGCGATTGT
TGCCGTTGGAAGTTTTGCCATTGCATTTGGTGAGGGTAAAATTTCACAGTGGATCAGTCCGTTTAAAGAAAATTCGGGCT
GTTTGAACCAGTTTTATCGGGAGGTTCCGCCTGTTCTGGTCAAAGGCAGTCTGCAAAGCAACAGCTATGCGTTATGCTTT
AATGGTTTTAATGTCATGTATTCGGGTGTCTCCAAAACACCGCTCTGGTCGGCAGAGCATTTAACGCCGCAACGTCTGAG
TACCAAGATCAAGCGTGAAGACAGTTTTCATGAAGAAACCCGAATTTCTAATGCATCGCATCGTGCGCTACTGAGTGACT
ATCGTGGTTCTGGTTATGACCGGGGGCATATGTCACCAAATGGTGATATGAGTGATACCAAAGCACAGCAGGACAGTTTT
TCGCTCGCCAATATGGTGCCGCAAGCGCCTAAAAATAATCAGCATATCTGGCGTGAACTGGAAGAAGCAACGCGTGCCCT
TGTGGTTAAGCAAAAGCAAGATGTGTTTGTGGTTACTGGACCTGTGTTTGAAGGTAAACGTCTTAAAACGATTGGTCGTG
GTGTGATTGTCCCAACGGCCGTTTATAAAGCAGTGTACTTACCTAAAACAGGTGCGATAGGTGCTTATTATGCGCCTAAT
AATAACTCACTTGAGGTACGTGTGGTGAGTGTATGTTATCTGGAAGAGAAACTGGGAATTAATCTGTTTCCACAACTGAC
CGAACAGCAGAAGCGCAATGTTTACCGCTTGCCGCTCACTGCGGGGCAGGTCAAGCCAGCACAGCCATTGAGCTATGCGC
ATTGGGATGGAGAGAGCCAGTGTGCAGAAGATATTAAGCCTGAAGGTATTGAGGCGACACAGAAAGCATTTAAACAACAA
ACCACTGAATCGGGCAGCTCGACATCAAGTACAGGGATTGATGAGGAAACCAAGCAAGCCCTGATTAAACAATTGGTCGA
AGCGCTCATGCATTATTTCTTACAAATTTTGAGATAA

Upstream 100 bases:

>100_bases
AGCAAATTGTGTTGCTGCTTTAACCGATCGACGCGGAGCTTGATATACAGCCCAAACCAGGCTTGTTAAGCTTTTTCTTT
TGGTTTTGATATGAAGCAGC

Downstream 100 bases:

>100_bases
ATACAGCCCGTTCACCAGTTGCATAAACGTGCAAAGCTGTTCAAGCTGAAAGCACTAAAACAACGATAAGTACAGGATGA
ATATGTTTAAACCTTATGAA

Product: DNA/RNA non-specific endonuclease G protein

Products: NA

Alternate protein names: Endonuclease [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MARKRSQTQSNSSWWNRYMGKFILAIVAVGSFAIAFGEGKISQWISPFKENSGCLNQFYREVPPVLVKGSLQSNSYALCF
NGFNVMYSGVSKTPLWSAEHLTPQRLSTKIKREDSFHEETRISNASHRALLSDYRGSGYDRGHMSPNGDMSDTKAQQDSF
SLANMVPQAPKNNQHIWRELEEATRALVVKQKQDVFVVTGPVFEGKRLKTIGRGVIVPTAVYKAVYLPKTGAIGAYYAPN
NNSLEVRVVSVCYLEEKLGINLFPQLTEQQKRNVYRLPLTAGQVKPAQPLSYAHWDGESQCAEDIKPEGIEATQKAFKQQ
TTESGSSTSSTGIDEETKQALIKQLVEALMHYFLQILR

Sequences:

>Translated_358_residues
MARKRSQTQSNSSWWNRYMGKFILAIVAVGSFAIAFGEGKISQWISPFKENSGCLNQFYREVPPVLVKGSLQSNSYALCF
NGFNVMYSGVSKTPLWSAEHLTPQRLSTKIKREDSFHEETRISNASHRALLSDYRGSGYDRGHMSPNGDMSDTKAQQDSF
SLANMVPQAPKNNQHIWRELEEATRALVVKQKQDVFVVTGPVFEGKRLKTIGRGVIVPTAVYKAVYLPKTGAIGAYYAPN
NNSLEVRVVSVCYLEEKLGINLFPQLTEQQKRNVYRLPLTAGQVKPAQPLSYAHWDGESQCAEDIKPEGIEATQKAFKQQ
TTESGSSTSSTGIDEETKQALIKQLVEALMHYFLQILR
>Mature_357_residues
ARKRSQTQSNSSWWNRYMGKFILAIVAVGSFAIAFGEGKISQWISPFKENSGCLNQFYREVPPVLVKGSLQSNSYALCFN
GFNVMYSGVSKTPLWSAEHLTPQRLSTKIKREDSFHEETRISNASHRALLSDYRGSGYDRGHMSPNGDMSDTKAQQDSFS
LANMVPQAPKNNQHIWRELEEATRALVVKQKQDVFVVTGPVFEGKRLKTIGRGVIVPTAVYKAVYLPKTGAIGAYYAPNN
NSLEVRVVSVCYLEEKLGINLFPQLTEQQKRNVYRLPLTAGQVKPAQPLSYAHWDGESQCAEDIKPEGIEATQKAFKQQT
TESGSSTSSTGIDEETKQALIKQLVEALMHYFLQILR

Specific function: Catalyzes the degradation of both RNA and DNA; has the potential to act as an endonuclease [H]

COG id: COG1864

COG function: function code F; DNA/RNA endonuclease G, NUC1

Gene ontology:

Cell location: Periplasm. Note=Periplasmic or loosely attached to the cytoplasmic or the outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

Organism=Homo sapiens, GI53759134, Length=212, Percent_Identity=34.4339622641509, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI224451073, Length=213, Percent_Identity=30.5164319248826, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI224451075, Length=160, Percent_Identity=34.375, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17505967, Length=216, Percent_Identity=27.3148148148148, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322253, Length=218, Percent_Identity=30.7339449541284, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI20129899, Length=213, Percent_Identity=30.0469483568075, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604
- InterPro:   IPR020821 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: 3.1.30.-

Molecular weight: Translated: 40099; Mature: 39968

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKRSQTQSNSSWWNRYMGKFILAIVAVGSFAIAFGEGKISQWISPFKENSGCLNQFYR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCHHHHHHHH
EVPPVLVKGSLQSNSYALCFNGFNVMYSGVSKTPLWSAEHLTPQRLSTKIKREDSFHEET
HCCCEEEECCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHH
RISNASHRALLSDYRGSGYDRGHMSPNGDMSDTKAQQDSFSLANMVPQAPKNNQHIWREL
HHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
EEATRALVVKQKQDVFVVTGPVFEGKRLKTIGRGVIVPTAVYKAVYLPKTGAIGAYYAPN
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCEEHHHHHHHHCCCCCCCCEEEECCC
NNSLEVRVVSVCYLEEKLGINLFPQLTEQQKRNVYRLPLTAGQVKPAQPLSYAHWDGESQ
CCCEEEEEEEEEEHHHHCCCCCCHHHHHHHHCCEEECCCCCCCCCCCCCCCEECCCCHHH
CAEDIKPEGIEATQKAFKQQTTESGSSTSSTGIDEETKQALIKQLVEALMHYFLQILR
HHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ARKRSQTQSNSSWWNRYMGKFILAIVAVGSFAIAFGEGKISQWISPFKENSGCLNQFYR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCHHHHHHHH
EVPPVLVKGSLQSNSYALCFNGFNVMYSGVSKTPLWSAEHLTPQRLSTKIKREDSFHEET
HCCCEEEECCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHH
RISNASHRALLSDYRGSGYDRGHMSPNGDMSDTKAQQDSFSLANMVPQAPKNNQHIWREL
HHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
EEATRALVVKQKQDVFVVTGPVFEGKRLKTIGRGVIVPTAVYKAVYLPKTGAIGAYYAPN
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCEEHHHHHHHHCCCCCCCCEEEECCC
NNSLEVRVVSVCYLEEKLGINLFPQLTEQQKRNVYRLPLTAGQVKPAQPLSYAHWDGESQ
CCCEEEEEEEEEEHHHHCCCCCCHHHHHHHHCCEEECCCCCCCCCCCCCCCEECCCCHHH
CAEDIKPEGIEATQKAFKQQTTESGSSTSSTGIDEETKQALIKQLVEALMHYFLQILR
HHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1343821; 11759840 [H]