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The map label for this gene is hisH

Identifier: 50086370

GI number: 50086370

Start: 3310094

End: 3310711

Strand: Direct

Name: hisH

Synonym: ACIAD3396

Alternate gene names: 50086370

Gene position: 3310094-3310711 (Clockwise)

Preceding gene: 161349957

Following gene: 50086371

Centisome position: 91.98

GC content: 43.53

Gene sequence:

>618_bases
ATGACCCGCATTGCCCTTCTTGATTATGGTATGGGAAATTTACACTCTGCTGCTAAAGCACTTGAGCATGTCGGGGCAAC
CGTAGATGTCACCAATGATCCAAAGCTGATTGCTAAAGCTGATAAAATCGTATTTCCAGGTGTGGGTGCAATGCGCGACT
GTATGCAGGGCATGCACGAAGCAGGCATTGATGAAGTGGTACGTCAGGCTGTATTTAACAAGCCTGTATTGGCCATCTGT
GTTGGCATGCAGGCACTGCTTGAAAGCTCTGAAGAAAATGGCGGTGTTCCTGCATTAGGTATTTTTGAAGGTGCAGTTAA
ACATTTTCCTGACCTTGGTCACTTAAAAGTTCCACATATGGGTTGGAATCAGGTATATCAGAACGATCCTGCTCACCCGA
TGTGGAACAATATCGATCAAGACAGCCGCTTTTACTTTGTACATAGTTATTATGTAGAACCCAAACAGTCTGATCTGATT
GCAGCAACCTGCGATTACGGTCTGCAATTTTGCACCGCAATTCATAAAGACAATTTGTTTGCCACCCAGTTTCATCCAGA
GAAAAGTCACACTGCTGGCTTACAACTGTTAAAAAACTTTGTGGAATGGAATATTTGA

Upstream 100 bases:

>100_bases
AAAGCGTGTTTAAAGCATTTGCACGTGCCTTACGTATGGCGTGTGAAATTGATCCTCGCGCGGCTGGCAGCATTGCTTCA
ACCAAAGGGAGCCTGTAATA

Downstream 100 bases:

>100_bases
TCAATACATCAGATCAAGCTGTCGAATGCAGCTTGATCTTTTCAAAAAAAATCAGGCACAATCGTAAAGCTTCTTCAGAT
TTATTCCAAATTTAAAACTG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTRIALLDYGMGNLHSAAKALEHVGATVDVTNDPKLIAKADKIVFPGVGAMRDCMQGMHEAGIDEVVRQAVFNKPVLAIC
VGMQALLESSEENGGVPALGIFEGAVKHFPDLGHLKVPHMGWNQVYQNDPAHPMWNNIDQDSRFYFVHSYYVEPKQSDLI
AATCDYGLQFCTAIHKDNLFATQFHPEKSHTAGLQLLKNFVEWNI

Sequences:

>Translated_205_residues
MTRIALLDYGMGNLHSAAKALEHVGATVDVTNDPKLIAKADKIVFPGVGAMRDCMQGMHEAGIDEVVRQAVFNKPVLAIC
VGMQALLESSEENGGVPALGIFEGAVKHFPDLGHLKVPHMGWNQVYQNDPAHPMWNNIDQDSRFYFVHSYYVEPKQSDLI
AATCDYGLQFCTAIHKDNLFATQFHPEKSHTAGLQLLKNFVEWNI
>Mature_204_residues
TRIALLDYGMGNLHSAAKALEHVGATVDVTNDPKLIAKADKIVFPGVGAMRDCMQGMHEAGIDEVVRQAVFNKPVLAICV
GMQALLESSEENGGVPALGIFEGAVKHFPDLGHLKVPHMGWNQVYQNDPAHPMWNNIDQDSRFYFVHSYYVEPKQSDLIA
ATCDYGLQFCTAIHKDNLFATQFHPEKSHTAGLQLLKNFVEWNI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=199, Percent_Identity=40.2010050251256, Blast_Score=147, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=33.1775700934579, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_ACIAD (Q6F7A7)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047880.1
- ProteinModelPortal:   Q6F7A7
- SMR:   Q6F7A7
- STRING:   Q6F7A7
- GeneID:   2878929
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3396
- NMPDR:   fig|62977.3.peg.3073
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- PhylomeDB:   Q6F7A7
- ProtClustDB:   PRK13141
- BioCyc:   ASP62977:ACIAD3396-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22720; Mature: 22589

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 80-80 ACT_SITE 185-185 ACT_SITE 187-187

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIALLDYGMGNLHSAAKALEHVGATVDVTNDPKLIAKADKIVFPGVGAMRDCMQGMHE
CCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCEEEECCCEECCCCHHHHHHHHHHHH
AGIDEVVRQAVFNKPVLAICVGMQALLESSEENGGVPALGIFEGAVKHFPDLGHLKVPHM
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEECCCC
GWNQVYQNDPAHPMWNNIDQDSRFYFVHSYYVEPKQSDLIAATCDYGLQFCTAIHKDNLF
CHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHHCCCEE
ATQFHPEKSHTAGLQLLKNFVEWNI
EEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRIALLDYGMGNLHSAAKALEHVGATVDVTNDPKLIAKADKIVFPGVGAMRDCMQGMHE
CEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCEEEECCCEECCCCHHHHHHHHHHHH
AGIDEVVRQAVFNKPVLAICVGMQALLESSEENGGVPALGIFEGAVKHFPDLGHLKVPHM
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEECCCC
GWNQVYQNDPAHPMWNNIDQDSRFYFVHSYYVEPKQSDLIAATCDYGLQFCTAIHKDNLF
CHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHHCCCEE
ATQFHPEKSHTAGLQLLKNFVEWNI
EEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA