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The map label for this gene is metY [H]

Identifier: 50086358

GI number: 50086358

Start: 3293919

End: 3295202

Strand: Direct

Name: metY [H]

Synonym: ACIAD3382

Alternate gene names: 50086358

Gene position: 3293919-3295202 (Clockwise)

Preceding gene: 50086350

Following gene: 50086359

Centisome position: 91.53

GC content: 44.7

Gene sequence:

>1284_bases
ATGACTCATCGCTACAAAGATGAAACCCTTGCTATTCATGCAGGTTATTCACCAGAACCAACCACGAAAGCTGTTGCTGT
ACCTATTTATCAAACCACTTCCTATGCTTTTGACAATACACAACATGGTGCAGATTTATTTGATCTGAAAGTACAGGGTA
ATATTTATACTCGAATCATGAATCCAACCACGGCCGTGCTCGAGCAGCGACTTGCCGCATTGGAAGGCGGAATTGGTGCT
TTGGCGCTGGCATCAGGTATGGCCGCCATTACCTATGCGATTCAGACGATAACCGAAGCAGGCGACAATATTGCATCTGT
TTCTACACTCTATGGGGGTACATATAATTTATTTGCGCATACCCTCCCCAAACAGGGCATTGAGGTACGTTTCTTCGATG
ATCAGCAGCCCGAGCAATTACGCAGTTTAATTGATGACAAAACCAAACTGGTTTTTGTGGAATCGATTGGCAATCCATTA
GGTAATATTATCGACTTGGAAACGATTGCCAAGATTGCCCATGAATATGGCGTCCCTGTGGTAGTCGACAATACTGTAGC
CACCCCTGTACTGCAAAAATCATTTGATTTTGGTGCGGATATTATTGTTCATTCACTGACCAAATATATTGGCGGTCATG
GCAACAGTATTGGTGGCATCATTGTCGATAGTGGCAAGTTTCCATGGGGCAAATATCCAGAACGCTTTAAGGTATTGAAT
ACCCCCGATCCAAGTTATCATGGTGTCAATTATGTCGAGGCCTTAGGTGAAGCTGCCTTCATAGGCCGTGCGCGTGTCGT
TCCATTACGCAATACAGGCGCAGCCATCAGTCCACTCAGTGTATTCCTAATTTTACAGGGACTCGAAACGCTATCCTTAC
GTATAGAGCGTCATACCGAAAATGCACTTCAAGTTGCTGAATACTTAAAGCAACACCCGAAGGTGAAATGGGTCAATTAT
GCTGGTTTAAAAGATCATCCTCAGCATGATTTGGCGCAAAAGTATGTCAAAGGAAAACCCTCTGCAATTCTTACTTTTGG
CGTGCAAGATGGTCGCGAAGGTGGCACTCGGTTTATTGATGCCTTACAACTGTTTACCCGTCTGGTCAATATTGGGGATG
CCAAGAGTCTGGCCTGTCATCCTGCCACGACCACGCATCGTCAGCTCAATGCAGAAGAACTTAAAGCAGCAGGCGTTTCA
GAAGATATGGTTCGGTTATCGGTTGGGATTGAACATATTGATGATTTGATTGCAGATCTGGAACAAGCATTGGCCACAGT
TTGA

Upstream 100 bases:

>100_bases
CTTAAAAAAGCTGAACTCCTCATAAAAATAGATAAATTAAGCATAAAGAATATGTTAAAAAATGAGCTTGCTGATTTTTT
ATACATCAATGGATAAAACA

Downstream 100 bases:

>100_bases
GCGTAAATTTTATAAAAAACCTCTGCAATTTCAGAGGTTTTTTTATATTTGCTTTATTATCGTATGATGTTCATAATTGA
TCTAGCAAATAATAAAAATT

Product: homocysteine synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTHRYKDETLAIHAGYSPEPTTKAVAVPIYQTTSYAFDNTQHGADLFDLKVQGNIYTRIMNPTTAVLEQRLAALEGGIGA
LALASGMAAITYAIQTITEAGDNIASVSTLYGGTYNLFAHTLPKQGIEVRFFDDQQPEQLRSLIDDKTKLVFVESIGNPL
GNIIDLETIAKIAHEYGVPVVVDNTVATPVLQKSFDFGADIIVHSLTKYIGGHGNSIGGIIVDSGKFPWGKYPERFKVLN
TPDPSYHGVNYVEALGEAAFIGRARVVPLRNTGAAISPLSVFLILQGLETLSLRIERHTENALQVAEYLKQHPKVKWVNY
AGLKDHPQHDLAQKYVKGKPSAILTFGVQDGREGGTRFIDALQLFTRLVNIGDAKSLACHPATTTHRQLNAEELKAAGVS
EDMVRLSVGIEHIDDLIADLEQALATV

Sequences:

>Translated_427_residues
MTHRYKDETLAIHAGYSPEPTTKAVAVPIYQTTSYAFDNTQHGADLFDLKVQGNIYTRIMNPTTAVLEQRLAALEGGIGA
LALASGMAAITYAIQTITEAGDNIASVSTLYGGTYNLFAHTLPKQGIEVRFFDDQQPEQLRSLIDDKTKLVFVESIGNPL
GNIIDLETIAKIAHEYGVPVVVDNTVATPVLQKSFDFGADIIVHSLTKYIGGHGNSIGGIIVDSGKFPWGKYPERFKVLN
TPDPSYHGVNYVEALGEAAFIGRARVVPLRNTGAAISPLSVFLILQGLETLSLRIERHTENALQVAEYLKQHPKVKWVNY
AGLKDHPQHDLAQKYVKGKPSAILTFGVQDGREGGTRFIDALQLFTRLVNIGDAKSLACHPATTTHRQLNAEELKAAGVS
EDMVRLSVGIEHIDDLIADLEQALATV
>Mature_426_residues
THRYKDETLAIHAGYSPEPTTKAVAVPIYQTTSYAFDNTQHGADLFDLKVQGNIYTRIMNPTTAVLEQRLAALEGGIGAL
ALASGMAAITYAIQTITEAGDNIASVSTLYGGTYNLFAHTLPKQGIEVRFFDDQQPEQLRSLIDDKTKLVFVESIGNPLG
NIIDLETIAKIAHEYGVPVVVDNTVATPVLQKSFDFGADIIVHSLTKYIGGHGNSIGGIIVDSGKFPWGKYPERFKVLNT
PDPSYHGVNYVEALGEAAFIGRARVVPLRNTGAAISPLSVFLILQGLETLSLRIERHTENALQVAEYLKQHPKVKWVNYA
GLKDHPQHDLAQKYVKGKPSAILTFGVQDGREGGTRFIDALQLFTRLVNIGDAKSLACHPATTTHRQLNAEELKAAGVSE
DMVRLSVGIEHIDDLIADLEQALATV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=419, Percent_Identity=35.0835322195704, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI299473759, Length=419, Percent_Identity=31.5035799522673, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI25453487, Length=418, Percent_Identity=30.622009569378, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=33.3333333333333, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1789383, Length=430, Percent_Identity=27.2093023255814, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17538055, Length=421, Percent_Identity=34.2042755344418, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI71980708, Length=417, Percent_Identity=30.2158273381295, Blast_Score=202, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=421, Percent_Identity=34.4418052256532, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=49.6535796766744, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319307, Length=432, Percent_Identity=31.25, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321254, Length=442, Percent_Identity=26.0180995475113, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=359, Percent_Identity=25.0696378830084, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=409, Percent_Identity=23.960880195599, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922554, Length=422, Percent_Identity=35.3080568720379, Blast_Score=222, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46468; Mature: 46337

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHRYKDETLAIHAGYSPEPTTKAVAVPIYQTTSYAFDNTQHGADLFDLKVQGNIYTRIM
CCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEC
NPTTAVLEQRLAALEGGIGALALASGMAAITYAIQTITEAGDNIASVSTLYGGTYNLFAH
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHH
TLPKQGIEVRFFDDQQPEQLRSLIDDKTKLVFVESIGNPLGNIIDLETIAKIAHEYGVPV
HCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEHHHCCCHHHHCCHHHHHHHHHHCCCCE
VVDNTVATPVLQKSFDFGADIIVHSLTKYIGGHGNSIGGIIVDSGKFPWGKYPERFKVLN
EECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEC
TPDPSYHGVNYVEALGEAAFIGRARVVPLRNTGAAISPLSVFLILQGLETLSLRIERHTE
CCCCCCCCCHHHHHHCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEHHHHH
NALQVAEYLKQHPKVKWVNYAGLKDHPQHDLAQKYVKGKPSAILTFGVQDGREGGTRFID
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
ALQLFTRLVNIGDAKSLACHPATTTHRQLNAEELKAAGVSEDMVRLSVGIEHIDDLIADL
HHHHHHHHHCCCCCCCEEECCCCCHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EQALATV
HHHHHCC
>Mature Secondary Structure 
THRYKDETLAIHAGYSPEPTTKAVAVPIYQTTSYAFDNTQHGADLFDLKVQGNIYTRIM
CCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEC
NPTTAVLEQRLAALEGGIGALALASGMAAITYAIQTITEAGDNIASVSTLYGGTYNLFAH
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHH
TLPKQGIEVRFFDDQQPEQLRSLIDDKTKLVFVESIGNPLGNIIDLETIAKIAHEYGVPV
HCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEHHHCCCHHHHCCHHHHHHHHHHCCCCE
VVDNTVATPVLQKSFDFGADIIVHSLTKYIGGHGNSIGGIIVDSGKFPWGKYPERFKVLN
EECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEC
TPDPSYHGVNYVEALGEAAFIGRARVVPLRNTGAAISPLSVFLILQGLETLSLRIERHTE
CCCCCCCCCHHHHHHCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEHHHHH
NALQVAEYLKQHPKVKWVNYAGLKDHPQHDLAQKYVKGKPSAILTFGVQDGREGGTRFID
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
ALQLFTRLVNIGDAKSLACHPATTTHRQLNAEELKAAGVSEDMVRLSVGIEHIDDLIADL
HHHHHHHHHCCCCCCCEEECCCCCHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EQALATV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]