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The map label for this gene is aroK

Identifier: 50086333

GI number: 50086333

Start: 3261760

End: 3262302

Strand: Reverse

Name: aroK

Synonym: ACIAD3354

Alternate gene names: 50086333

Gene position: 3262302-3261760 (Counterclockwise)

Preceding gene: 50086334

Following gene: 50086332

Centisome position: 90.65

GC content: 41.99

Gene sequence:

>543_bases
TTGCCAAGCAAAGAGTTTGACACCCTGCCAAATATTTATTTGGTAGGGCCCATGGGAGCAGGAAAGACAACAGTCGGTCG
ACATTTAGCAGAGTTGTTAGGGCGTGAGTTTCTAGACAGTGATCATGAAATTGAGCGTAGAACAGGTGCAACCATCCCAT
GGATTTTTGAAAAAGAGGGTGAAATCGGATTTAGAAGTCGGGAAACCATTGTGCTGGATGATTTAACATCTCGACGGGAT
TTAGTGTTAGCAACAGGTGGTGGGGTGGTAACACAACCAGCCAACCGGAGCTATTTAAAAACACGAGGTGTTGTTGTTTA
TTTATACACGCCTGTTGAGCTTCAGTTACAGCGTACCTATCGTGATAAAAATAGACCTTTATTACAGGTTGAAAACCCTG
AACAGCGTCTTCGTGATTTATTAAAGTTAAGAGATCCTTTATATCGCGATGTTGCACATCATATTATCGAAACAAATCAA
GGTGCTGCCCGAGAGCTGGCTCAGCAAATTTTACGTGTGATTTTAAGTTCTGGATCACATTAA

Upstream 100 bases:

>100_bases
AAATGAGCTGCTAATTTTTGTGACGCCTCGAATAGTTAATGACACTATTGTTGGAAATCATTAATATAGTTTTAATTCAA
GTAATTCAATAGGTGACTCC

Downstream 100 bases:

>100_bases
GTTTTTACTGGTTTAAATTTTATGCAAACTCTACACGTTGAATTAGGCGATCGCCGTTATCCGATATTTATTGGTAGTCA
GCTAAATCCTCAGAATCTAC

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MPSKEFDTLPNIYLVGPMGAGKTTVGRHLAELLGREFLDSDHEIERRTGATIPWIFEKEGEIGFRSRETIVLDDLTSRRD
LVLATGGGVVTQPANRSYLKTRGVVVYLYTPVELQLQRTYRDKNRPLLQVENPEQRLRDLLKLRDPLYRDVAHHIIETNQ
GAARELAQQILRVILSSGSH

Sequences:

>Translated_180_residues
MPSKEFDTLPNIYLVGPMGAGKTTVGRHLAELLGREFLDSDHEIERRTGATIPWIFEKEGEIGFRSRETIVLDDLTSRRD
LVLATGGGVVTQPANRSYLKTRGVVVYLYTPVELQLQRTYRDKNRPLLQVENPEQRLRDLLKLRDPLYRDVAHHIIETNQ
GAARELAQQILRVILSSGSH
>Mature_179_residues
PSKEFDTLPNIYLVGPMGAGKTTVGRHLAELLGREFLDSDHEIERRTGATIPWIFEKEGEIGFRSRETIVLDDLTSRRDL
VLATGGGVVTQPANRSYLKTRGVVVYLYTPVELQLQRTYRDKNRPLLQVENPEQRLRDLLKLRDPLYRDVAHHIIETNQG
AARELAQQILRVILSSGSH

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=168, Percent_Identity=52.9761904761905, Blast_Score=174, Evalue=2e-45,
Organism=Escherichia coli, GI1786587, Length=153, Percent_Identity=40.5228758169935, Blast_Score=114, Evalue=5e-27,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_ACIAD (Q6F7E4)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047843.1
- ProteinModelPortal:   Q6F7E4
- SMR:   Q6F7E4
- STRING:   Q6F7E4
- GeneID:   2878790
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3354
- NMPDR:   fig|62977.3.peg.3036
- eggNOG:   COG0703
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- PhylomeDB:   Q6F7E4
- ProtClustDB:   PRK00131
- BioCyc:   ASP62977:ACIAD3354-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 20471; Mature: 20340

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 41-41 BINDING 65-65 BINDING 87-87 BINDING 125-125 BINDING 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSKEFDTLPNIYLVGPMGAGKTTVGRHLAELLGREFLDSDHEIERRTGATIPWIFEKEG
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEECCC
EIGFRSRETIVLDDLTSRRDLVLATGGGVVTQPANRSYLKTRGVVVYLYTPVELQLQRTY
CCCCCCCCEEEEECCCCCCCEEEEECCCEEECCCCCCEEECCCEEEEEECCCEEEHHHHH
RDKNRPLLQVENPEQRLRDLLKLRDPLYRDVAHHIIETNQGAARELAQQILRVILSSGSH
HCCCCCEEEECCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PSKEFDTLPNIYLVGPMGAGKTTVGRHLAELLGREFLDSDHEIERRTGATIPWIFEKEG
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEECCC
EIGFRSRETIVLDDLTSRRDLVLATGGGVVTQPANRSYLKTRGVVVYLYTPVELQLQRTY
CCCCCCCCEEEEECCCCCCCEEEEECCCEEECCCCCCEEECCCEEEEEECCCEEEHHHHH
RDKNRPLLQVENPEQRLRDLLKLRDPLYRDVAHHIIETNQGAARELAQQILRVILSSGSH
HCCCCCEEEECCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA