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The map label for this gene is gltD [H]

Identifier: 50086329

GI number: 50086329

Start: 3253359

End: 3254780

Strand: Reverse

Name: gltD [H]

Synonym: ACIAD3349

Alternate gene names: 50086329

Gene position: 3254780-3253359 (Counterclockwise)

Preceding gene: 50086330

Following gene: 50086328

Centisome position: 90.45

GC content: 45.5

Gene sequence:

>1422_bases
ATGGCAGAGCGCCTAAATAATGACTTTCAGTTTCTGGATGTAGCACGCCAAGATCCAGAGAAAAAAGATATTGATGTCCG
CAAATCTGAGTTTGTGGAAATTTACAAACCATTTACTGCTGAAGTTGCTGCAAATCAGACGCATCGTTGTTTGGGCTGTG
GTAATCCGTACTGTGAATGGAAATGCCCAGTACATAACTATATTCCAAACTGGTTGAAGTTGATTGCAGAGGGCCGTATT
TTTCAGGCGGCTGAATTGTGTCATCAAACCAATACTTTGCCTGAAGTGTGTGGTCGTGTTTGTCCGCAAGACCGCTTGTG
TGAAGGTGCATGTACATTGAATGACGGCTTTGGTGCTGTCACAATTGGTAATGCAGAAAAATATATTAATGACACCGCTT
TTGCATTGGGCTGGCGTCCAGATATGTCTTCAGTTAAATGGACCAATAAAAAAGTTGCGATTATTGGTGCAGGCCCTGCG
GGCTTGGGCTGTGCAGATATTCTGGTGCGTAATGGTGTAAAGCCAGTGGTATTTGACAAGCGTCCAGAAATTGGTGGATT
GCTGACCTTTGGTATTCCAGAATTCAAGATGGAAAAAGATGTTATGAAACGCCGCCGTGAAATTTTCACGGGTATGGGGG
TTGAGTTCCGTCTAAATGTTGAAATTGGTAAAGATGTCACAATCGAGCAATTGCTTGAAGACTATGATGCTGTATTCATG
GGTATGGGCACCTACACCTATATGAAAGGTGGCTTCCCAGGTGAAGACTTAGATGGCGTATACGATGCACTCGACTTTTT
GATTGCCAATGTTAACCGTACTCAGGGCTGGGAGCAAAATCCGAGCGATTATATTAGTGTTGAAGGCAAAAAAGTGATTG
TATTGGGTGGCGGTGATACAGCGATGGACTGTAACCGTACCTCCATTCGTCAAGGTGCTGAAGAAGTAACGTGTGCATAC
CGTCGTGACGAAGCCAATATGCCAGGTTCACGTCGTGAAGTACAAAATGCACGTGAAGAAGGCGTCAACTTCCTGTTTAA
TCGCCAGCCTATCGAGATTGTAGGTGAAAATGGTCAGGTGACAGGCGTAAAAGTGGTCACTACTCAGCTTGGAGCTCCAG
ACAGCCGTGGCCGTCGTAGTCCAGAGCCAATTCCAGGTTCGGAAGAAGTGCTTGCAGCCGATGCGGTGCTACTTGCTTTT
GGTTTCCGTCCTAGTCCAGCAGATTGGTTTGCATCATCAAGTATTAACTTGGATGGTTCAGGTCGTGTAGTGGCAGCAGA
ACAGCAACAATATAAATTCCAGACATCTAACCCAAAAATCTTTGCGGGTGGCGATATGGTACGTGGTTCTGATCTGGTGG
TAACTGCAATTTGGGAAGGCCGTGAGGCCGCAGAAGGTATTTTGGATTACCTTGATGTCTAA

Upstream 100 bases:

>100_bases
AAGCGACTAAAGCAGATCCTCAATAATTCCACTTACTCATATTTTGATAGGCAGGTGTGGATAACTTTCGAGTTTTCCAC
ATTGACCCACGGAGCAAGAC

Downstream 100 bases:

>100_bases
CTTTTAAATATTTAAAGATCCATTTCAAAAGCCTGCAAAGATGCAGGCTTTTGTGTTTTTGTATGACACATACCCAGATT
GAGCAATTCTGTCAATTTAC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MAERLNNDFQFLDVARQDPEKKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCGNPYCEWKCPVHNYIPNWLKLIAEGRI
FQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNAEKYINDTAFALGWRPDMSSVKWTNKKVAIIGAGPA
GLGCADILVRNGVKPVVFDKRPEIGGLLTFGIPEFKMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFM
GMGTYTYMKGGFPGEDLDGVYDALDFLIANVNRTQGWEQNPSDYISVEGKKVIVLGGGDTAMDCNRTSIRQGAEEVTCAY
RRDEANMPGSRREVQNAREEGVNFLFNRQPIEIVGENGQVTGVKVVTTQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAF
GFRPSPADWFASSSINLDGSGRVVAAEQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLDV

Sequences:

>Translated_473_residues
MAERLNNDFQFLDVARQDPEKKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCGNPYCEWKCPVHNYIPNWLKLIAEGRI
FQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNAEKYINDTAFALGWRPDMSSVKWTNKKVAIIGAGPA
GLGCADILVRNGVKPVVFDKRPEIGGLLTFGIPEFKMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFM
GMGTYTYMKGGFPGEDLDGVYDALDFLIANVNRTQGWEQNPSDYISVEGKKVIVLGGGDTAMDCNRTSIRQGAEEVTCAY
RRDEANMPGSRREVQNAREEGVNFLFNRQPIEIVGENGQVTGVKVVTTQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAF
GFRPSPADWFASSSINLDGSGRVVAAEQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLDV
>Mature_472_residues
AERLNNDFQFLDVARQDPEKKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCGNPYCEWKCPVHNYIPNWLKLIAEGRIF
QAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNAEKYINDTAFALGWRPDMSSVKWTNKKVAIIGAGPAG
LGCADILVRNGVKPVVFDKRPEIGGLLTFGIPEFKMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFMG
MGTYTYMKGGFPGEDLDGVYDALDFLIANVNRTQGWEQNPSDYISVEGKKVIVLGGGDTAMDCNRTSIRQGAEEVTCAYR
RDEANMPGSRREVQNAREEGVNFLFNRQPIEIVGENGQVTGVKVVTTQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAFG
FRPSPADWFASSSINLDGSGRVVAAEQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLDV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=475, Percent_Identity=29.6842105263158, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI111118983, Length=213, Percent_Identity=27.6995305164319, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI111118981, Length=165, Percent_Identity=29.6969696969697, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789606, Length=469, Percent_Identity=69.0831556503198, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=462, Percent_Identity=60.1731601731602, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI87082180, Length=462, Percent_Identity=54.1125541125541, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI1788468, Length=440, Percent_Identity=30.9090909090909, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1789243, Length=421, Percent_Identity=30.6413301662708, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17570289, Length=496, Percent_Identity=34.0725806451613, Blast_Score=219, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI71984108, Length=465, Percent_Identity=29.247311827957, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17543860, Length=227, Percent_Identity=27.3127753303965, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=491, Percent_Identity=31.9755600814664, Blast_Score=223, Evalue=5e-59,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=37.319587628866, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=37.319587628866, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=37.319587628866, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=37.319587628866, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24640763, Length=439, Percent_Identity=28.0182232346241, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI18858217, Length=439, Percent_Identity=28.0182232346241, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52186; Mature: 52054

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERLNNDFQFLDVARQDPEKKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCGNPYCEW
CCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHCCCCCEEEECCCCCCEE
KCPVHNYIPNWLKLIAEGRIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAV
ECCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCEE
TIGNAEKYINDTAFALGWRPDMSSVKWTNKKVAIIGAGPAGLGCADILVRNGVKPVVFDK
EECCHHHHHCCCEEEEECCCCCCCCEECCCEEEEEECCCCCCCHHHHHHHCCCCCEEECC
RPEIGGLLTFGIPEFKMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFM
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHEE
GMGTYTYMKGGFPGEDLDGVYDALDFLIANVNRTQGWEQNPSDYISVEGKKVIVLGGGDT
CCCHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEECCCCC
AMDCNRTSIRQGAEEVTCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPIEIVGENGQV
CCCCCHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEECCCCEEEECCCCCE
TGVKVVTTQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAFGFRPSPADWFASSSINLDGS
EEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHEEEEEECCCCCCHHHHCCCCCCCCCC
GRVVAAEQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLDV
CCEEEECCCCEEEECCCCEEEECCCEECCCCEEEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
AERLNNDFQFLDVARQDPEKKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCGNPYCEW
CCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHCCCCCEEEECCCCCCEE
KCPVHNYIPNWLKLIAEGRIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAV
ECCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCEE
TIGNAEKYINDTAFALGWRPDMSSVKWTNKKVAIIGAGPAGLGCADILVRNGVKPVVFDK
EECCHHHHHCCCEEEEECCCCCCCCEECCCEEEEEECCCCCCCHHHHHHHCCCCCEEECC
RPEIGGLLTFGIPEFKMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFM
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHEE
GMGTYTYMKGGFPGEDLDGVYDALDFLIANVNRTQGWEQNPSDYISVEGKKVIVLGGGDT
CCCHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEECCCCC
AMDCNRTSIRQGAEEVTCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPIEIVGENGQV
CCCCCHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEECCCCEEEECCCCCE
TGVKVVTTQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAFGFRPSPADWFASSSINLDGS
EEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHEEEEEECCCCCCHHHHCCCCCCCCCC
GRVVAAEQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLDV
CCEEEECCCCEEEECCCCEEEECCCEECCCCEEEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]