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The map label for this gene is lip [H]

Identifier: 50086294

GI number: 50086294

Start: 3216014

End: 3216982

Strand: Direct

Name: lip [H]

Synonym: ACIAD3309

Alternate gene names: 50086294

Gene position: 3216014-3216982 (Clockwise)

Preceding gene: 50086293

Following gene: 50086307

Centisome position: 89.37

GC content: 41.8

Gene sequence:

>969_bases
ATGAAATTCAAATTATTATTTACAACTTTATTGCTGGTTCTAACCCAACCTGTATTTGCAACCAGTCCAATTCAGAATCC
AACTACATCTTTTGTCATATCCGATTATGCAAAGACCAAATATCCGATTGTTCTCTCACATGGACTATTCGGTTTTAATA
AATTAGGCACAGAAGCCTTTGGGTTAGATTATTGGTATCAGATTCCACAAGATTTGGCCAGAAATGGTGCCAATGTCTGG
GTAACCCGTCAATCTACTGCCAATACATCTGAATTTCGAGGCGAACAATTACTTGCTGAAGTTCAGGACATTCTGGCCAT
TACAGGCGCACAAAAAGTTAATCTGATTGGGCATAGTCATGGCTCCCAAACTGTACGCTATGTTGCTGGTGTTCTCCCTG
CAAATATTGCTTCCGTTTCAACGATTGGTGGCCCTGCCAAAGGTGCCCCACTGGCTGATCTGATTTATAAAACACTGGCT
GGCACACCATTAGAAGCGCCTATTGCGACCATTTTCAATGTCGCAATGAATTTTATTACGATAGGACAATTCGATGATCC
GCAGAAATATCCAATGAACTCTGTCGGTGCAGCGTATAGTCTATCCACTGAAGGTGCTGGTAAATTTAATGCAATCTTTC
CTGCTGGCGTACCAACGACTGCATGCGGTCAAGGTGAATCTTCTGTCAATGGTGTACGTTATTATTCATGGAGCGGTGCT
TCTCCATTAACCAATCCACTCGATCCTTCAGATTATGGCCTGAGCTTGACCAGTGTATTTAGTGGCAAAAACAATGACGG
GCTGGTACCTTCATGTAGCAGTCACTTGGGTACAGTAATTCGAGATAATTACGTATGGAATCATCTGGATGAAGTCAATC
AAATTCTGGGTTTACGATCTATTTTTGCACAAGACCCCGTATCCATCTTTAGACAACATGCCAATCGTCTCAAAGGTCAA
AATCTATAA

Upstream 100 bases:

>100_bases
AGACCTTAATTTAGCTTTACCCGTGTTTTAACGGAAATAATATGCGGTTTGAAAATGGATGAATTTTCAAACTCAGAAAA
ATGAAATTTAGGGAAATAAA

Downstream 100 bases:

>100_bases
TTAAGATGCACCAGATGTAAAAAATGCGCCCAATCAGGCGCATTTTTATGCTGATAAGCGAAGTTAAAATTAACCTCGTT
TACGCGCTGGCAATTTTTCA

Product: lipase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKFKLLFTTLLLVLTQPVFATSPIQNPTTSFVISDYAKTKYPIVLSHGLFGFNKLGTEAFGLDYWYQIPQDLARNGANVW
VTRQSTANTSEFRGEQLLAEVQDILAITGAQKVNLIGHSHGSQTVRYVAGVLPANIASVSTIGGPAKGAPLADLIYKTLA
GTPLEAPIATIFNVAMNFITIGQFDDPQKYPMNSVGAAYSLSTEGAGKFNAIFPAGVPTTACGQGESSVNGVRYYSWSGA
SPLTNPLDPSDYGLSLTSVFSGKNNDGLVPSCSSHLGTVIRDNYVWNHLDEVNQILGLRSIFAQDPVSIFRQHANRLKGQ
NL

Sequences:

>Translated_322_residues
MKFKLLFTTLLLVLTQPVFATSPIQNPTTSFVISDYAKTKYPIVLSHGLFGFNKLGTEAFGLDYWYQIPQDLARNGANVW
VTRQSTANTSEFRGEQLLAEVQDILAITGAQKVNLIGHSHGSQTVRYVAGVLPANIASVSTIGGPAKGAPLADLIYKTLA
GTPLEAPIATIFNVAMNFITIGQFDDPQKYPMNSVGAAYSLSTEGAGKFNAIFPAGVPTTACGQGESSVNGVRYYSWSGA
SPLTNPLDPSDYGLSLTSVFSGKNNDGLVPSCSSHLGTVIRDNYVWNHLDEVNQILGLRSIFAQDPVSIFRQHANRLKGQ
NL
>Mature_322_residues
MKFKLLFTTLLLVLTQPVFATSPIQNPTTSFVISDYAKTKYPIVLSHGLFGFNKLGTEAFGLDYWYQIPQDLARNGANVW
VTRQSTANTSEFRGEQLLAEVQDILAITGAQKVNLIGHSHGSQTVRYVAGVLPANIASVSTIGGPAKGAPLADLIYKTLA
GTPLEAPIATIFNVAMNFITIGQFDDPQKYPMNSVGAAYSLSTEGAGKFNAIFPAGVPTTACGQGESSVNGVRYYSWSGA
SPLTNPLDPSDYGLSLTSVFSGKNNDGLVPSCSSHLGTVIRDNYVWNHLDEVNQILGLRSIFAQDPVSIFRQHANRLKGQ
NL

Specific function: Catalyzes the hydrolysis of triglycerides. Catalyzes the synthesis of macrocyclic lactones in anhydrous organic solvents [H]

COG id: COG1075

COG function: function code R; Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold

Gene ontology:

Cell location: Cell surface. Secreted, extracellular space. Note=During early stationary growth phase about 10% of the enzyme molecules remain cell-bound while about 90% are released into the growth medium [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Pseudomonas lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 34623; Mature: 34623

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKLLFTTLLLVLTQPVFATSPIQNPTTSFVISDYAKTKYPIVLSHGLFGFNKLGTEAF
CCHHHHHHHHHHHHCCCHHHCCCCCCCCCHHEEEHHHCCCCCEEEECCCCCHHHHCCHHH
GLDYWYQIPQDLARNGANVWVTRQSTANTSEFRGEQLLAEVQDILAITGAQKVNLIGHSH
CHHHHHHCHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
GSQTVRYVAGVLPANIASVSTIGGPAKGAPLADLIYKTLAGTPLEAPIATIFNVAMNFIT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHEE
IGQFDDPQKYPMNSVGAAYSLSTEGAGKFNAIFPAGVPTTACGQGESSVNGVRYYSWSGA
CCCCCCCCCCCCHHCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCC
SPLTNPLDPSDYGLSLTSVFSGKNNDGLVPSCSSHLGTVIRDNYVWNHLDEVNQILGLRS
CCCCCCCCCCCCCEEEEHEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IFAQDPVSIFRQHANRLKGQNL
HHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKFKLLFTTLLLVLTQPVFATSPIQNPTTSFVISDYAKTKYPIVLSHGLFGFNKLGTEAF
CCHHHHHHHHHHHHCCCHHHCCCCCCCCCHHEEEHHHCCCCCEEEECCCCCHHHHCCHHH
GLDYWYQIPQDLARNGANVWVTRQSTANTSEFRGEQLLAEVQDILAITGAQKVNLIGHSH
CHHHHHHCHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
GSQTVRYVAGVLPANIASVSTIGGPAKGAPLADLIYKTLAGTPLEAPIATIFNVAMNFIT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHEE
IGQFDDPQKYPMNSVGAAYSLSTEGAGKFNAIFPAGVPTTACGQGESSVNGVRYYSWSGA
CCCCCCCCCCCCHHCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCC
SPLTNPLDPSDYGLSLTSVFSGKNNDGLVPSCSSHLGTVIRDNYVWNHLDEVNQILGLRS
CCCCCCCCCCCCCEEEEHEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IFAQDPVSIFRQHANRLKGQNL
HHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1512563; 1632642; 1576157; 10984043; 1748875; 11133953 [H]