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The map label for this gene is mdfA [H]

Identifier: 50086279

GI number: 50086279

Start: 3201357

End: 3202586

Strand: Reverse

Name: mdfA [H]

Synonym: ACIAD3292

Alternate gene names: 50086279

Gene position: 3202586-3201357 (Counterclockwise)

Preceding gene: 50086283

Following gene: 50086274

Centisome position: 88.99

GC content: 43.9

Gene sequence:

>1230_bases
ATGCAAAATGTTCGAACAACAACATTAAACCGCACAACGTTAATGTTTCCTTTGGCTCTGGTTTTATTCGAGTTTGCGGT
ATACATCGGAAACGATTTAATTCAGCCTGCCATGCTGGCCATTACTCAGGATTTTGCTGTGAGTGCCACTTGGGCACCTT
CTTCGATGTCCTTTTATTTGCTGGGTGGTGCATGTGTGGCTTGGTTACTCGGCCCATTGTCTGATCGTCTTGGGCGTAAA
CGTGTACTGCTATTTGGTGTGCTATTTTTTGCGCTGTGCTGTCTTCTGATTTTACTGACACACAATATGGCACAATTTTT
GGCATTACGTTTTTTACAAGGCATTGGCTTAAGTGTGATTACTGCGGTCGGTTATGCGGCAATTCAGGAAACCTTTGAGG
AGCGTGATGCGATTAAAGTCATGGCTTTAATGGCAAATATCTCGTTACTTGCGCCATTACTTGGCCCCGTCTTGGGTGCT
TTTCTCATTGATTATGTATCTTGGCATTGGGGGTTCGTTGCAATTGCGACTCTGGCTGGTTTAAGTTGGTTTGGCCTTAA
AGCCAAAATGCCAAGTCAGCAACACAGTGTGACCCGTCAGCCTTTCGTGTATCTATTTGATGATTTTAAAAAGATCTATT
TGGATAAACGCTTTCTTGGCTTAAGTCTGGGGCTGCCACTGGTCGGGATGCCACTGATGCTCTGGATTGCATTATCCCCC
GTGATGTTGGTCGATGAACTTAAATTTAGCAGTGTGCAATATGGTCTGGCGCAGTTTCCTATTTTTATGGGTTTAATTCT
GGGAAATATTGTGCTCATCAAGATCATAGATCGTTTTCCGCTTGGAAAAACCATTTTAATGGGCTTACCGATTATGCTCG
TAGGAACACTGGTTTTGCTGGTGGGCGCAATCTGGCAAGACTATCTGGCAGTTTGCCTCTTGGTTGGCATGACGCTGATC
AGTTTTGGTGAAGGGATTAGTTTTTCGGTTATTTACCGCTTTGCATTGATGTCTTCCCCAGTTGCCAAGGGAACAGTTGC
CGCTGCGGTCTCGATGCTTTTAATGTTTAGCTTTTTTATCATGATTGAACTGGTACGTGAGCTGTATGTGCATTTTCATG
TGCTGGCATTTAGTCTGTCTTGTGTGGTCTTGATTACACTCTGGTTTTTGCTGCCACGACTGATGCTTAAACAGGAAATG
GCAATCCGTCATACACATGATCAGTTCTAA

Upstream 100 bases:

>100_bases
AAATCAATTCTTTTTCCTTGGTGCTTGTCATCAGGCTGAGGCATTGTTCATCAAAAATATGCTCAAGCCATGACTCCCCA
ATATTGATTAGTAGATCACA

Downstream 100 bases:

>100_bases
TCTGTTTGAAGCTCTGATATGTCTGGACGGAAAGCGCCAGACATATCGACGGATTAACGAATCTCTTTTTCAAGGAGTTG
GCGACGCAGTGTTACCAGTT

Product: major facilitator superfamily multidrug/chloramphenicol efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MQNVRTTTLNRTTLMFPLALVLFEFAVYIGNDLIQPAMLAITQDFAVSATWAPSSMSFYLLGGACVAWLLGPLSDRLGRK
RVLLFGVLFFALCCLLILLTHNMAQFLALRFLQGIGLSVITAVGYAAIQETFEERDAIKVMALMANISLLAPLLGPVLGA
FLIDYVSWHWGFVAIATLAGLSWFGLKAKMPSQQHSVTRQPFVYLFDDFKKIYLDKRFLGLSLGLPLVGMPLMLWIALSP
VMLVDELKFSSVQYGLAQFPIFMGLILGNIVLIKIIDRFPLGKTILMGLPIMLVGTLVLLVGAIWQDYLAVCLLVGMTLI
SFGEGISFSVIYRFALMSSPVAKGTVAAAVSMLLMFSFFIMIELVRELYVHFHVLAFSLSCVVLITLWFLLPRLMLKQEM
AIRHTHDQF

Sequences:

>Translated_409_residues
MQNVRTTTLNRTTLMFPLALVLFEFAVYIGNDLIQPAMLAITQDFAVSATWAPSSMSFYLLGGACVAWLLGPLSDRLGRK
RVLLFGVLFFALCCLLILLTHNMAQFLALRFLQGIGLSVITAVGYAAIQETFEERDAIKVMALMANISLLAPLLGPVLGA
FLIDYVSWHWGFVAIATLAGLSWFGLKAKMPSQQHSVTRQPFVYLFDDFKKIYLDKRFLGLSLGLPLVGMPLMLWIALSP
VMLVDELKFSSVQYGLAQFPIFMGLILGNIVLIKIIDRFPLGKTILMGLPIMLVGTLVLLVGAIWQDYLAVCLLVGMTLI
SFGEGISFSVIYRFALMSSPVAKGTVAAAVSMLLMFSFFIMIELVRELYVHFHVLAFSLSCVVLITLWFLLPRLMLKQEM
AIRHTHDQF
>Mature_409_residues
MQNVRTTTLNRTTLMFPLALVLFEFAVYIGNDLIQPAMLAITQDFAVSATWAPSSMSFYLLGGACVAWLLGPLSDRLGRK
RVLLFGVLFFALCCLLILLTHNMAQFLALRFLQGIGLSVITAVGYAAIQETFEERDAIKVMALMANISLLAPLLGPVLGA
FLIDYVSWHWGFVAIATLAGLSWFGLKAKMPSQQHSVTRQPFVYLFDDFKKIYLDKRFLGLSLGLPLVGMPLMLWIALSP
VMLVDELKFSSVQYGLAQFPIFMGLILGNIVLIKIIDRFPLGKTILMGLPIMLVGTLVLLVGAIWQDYLAVCLLVGMTLI
SFGEGISFSVIYRFALMSSPVAKGTVAAAVSMLLMFSFFIMIELVRELYVHFHVLAFSLSCVVLITLWFLLPRLMLKQEM
AIRHTHDQF

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=410, Percent_Identity=41.7073170731707, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1790794, Length=413, Percent_Identity=35.3510895883777, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI48994889, Length=375, Percent_Identity=26.4, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788509, Length=207, Percent_Identity=27.536231884058, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790146, Length=314, Percent_Identity=27.3885350318471, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790195, Length=140, Percent_Identity=30, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319480, Length=171, Percent_Identity=25.7309941520468, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45515; Mature: 45515

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]