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The map label for this gene is rpoA

Identifier: 50086190

GI number: 50086190

Start: 3121923

End: 3122930

Strand: Reverse

Name: rpoA

Synonym: ACIAD3194

Alternate gene names: 50086190

Gene position: 3122930-3121923 (Counterclockwise)

Preceding gene: 50086191

Following gene: 50086189

Centisome position: 86.78

GC content: 44.35

Gene sequence:

>1008_bases
ATGACGCGTACTGCAAACGAGTTTCTAACTCCGCAAGCGATCAAGGTCGAAGCGGTGAGCGGGACCTCGGCAAAAGTGAT
TCTGGAACCTTTAGAGCGTGGCTTTGGTCACACTCTAGGTAATGCTTTACGTCGCATTCTATTGTCTTCTTTGCCTGGCG
CTGCTGTGGTTGAAGTTGAAATAGAAGGCGTCGAGCACGAGTACAGTACTTTAGAAGGCTTGCAGCAGGACATCGTCGAG
CTCTTGCTGAACCTTAAAGGATTGTCTATTAAGCTGTTCGATCAAAATGAAGCATATTTGACATTGGAGAAACAAGGGCC
AGGCGACATTACTGCCGCTGATCTTCGTTTGCCTCATAATGTTGAAGTGGTCAATCCAGAACATCTTATTGGTACTTTGA
GTGCTTCAGGCTCAATCAAAATGCGCCTAAAAGTATCTCAAGGTCGTGGCTATGAAACTTCTGACTCGCGTTTCCCGGAA
GGAGAAACTCGTCCGGTAGGTCGCCTACAGTTAGATGCTTCTTATAGCCCGATCAAACGTGTATCTTACACCGTTGAAAA
TGCGCGTGTAGAACAACGTACCGATCTTGATAAACTGGTCATTGATCTTGAAACCAATGGAACTGTAGATCCTGAAGAAG
CAATCCGCAAAGCGGCAACAATCTTGCAACAACAAATTGCAATTTTTGTTGATCTTCAGAAAGATCAAGCTCCAGTGGCT
CAAGAACCTCGTGAAGAAGTTGACCCAATCTTGCTTCGTCCAGTAGATGATCTAGAGCTTACTGTTCGTTCTGCTAACTG
TTTGAAAGCAGAAAATATTTACTACATTGGTGATCTTGTTCAACGTACTGAAGTTGAGTTGTTAAAAACTCCAAACCTAG
GTAAAAAATCGTTGACTGAGATTAAAGATGTTTTAGCTTCGAAAGGCTTGCAACTCGGCATGCGTTTAGAAAACTGGCCA
CCTGCTAGTTTACGCATGGACGATCGCTTTGCCTATCGCAGCCGTTAA

Upstream 100 bases:

>100_bases
TACGTTCAAAGCTGCGCCAGATCGTTCTGATTTGCCTGCTGAAATCAACGAAAGCTTGATTGTAGAATTGTATTCTAAAT
AATCCTTGAGGTAGCAATAA

Downstream 100 bases:

>100_bases
TTAAGTAGGACTATCACCATGCGTCATCGTAATAGTGGTGTGAAATTAGGCCGTACAAGCAGCCATCGTAAAGCGATGTT
CCAAAACTTGGCGAATTCAT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTRTANEFLTPQAIKVEAVSGTSAKVILEPLERGFGHTLGNALRRILLSSLPGAAVVEVEIEGVEHEYSTLEGLQQDIVE
LLLNLKGLSIKLFDQNEAYLTLEKQGPGDITAADLRLPHNVEVVNPEHLIGTLSASGSIKMRLKVSQGRGYETSDSRFPE
GETRPVGRLQLDASYSPIKRVSYTVENARVEQRTDLDKLVIDLETNGTVDPEEAIRKAATILQQQIAIFVDLQKDQAPVA
QEPREEVDPILLRPVDDLELTVRSANCLKAENIYYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASKGLQLGMRLENWP
PASLRMDDRFAYRSR

Sequences:

>Translated_335_residues
MTRTANEFLTPQAIKVEAVSGTSAKVILEPLERGFGHTLGNALRRILLSSLPGAAVVEVEIEGVEHEYSTLEGLQQDIVE
LLLNLKGLSIKLFDQNEAYLTLEKQGPGDITAADLRLPHNVEVVNPEHLIGTLSASGSIKMRLKVSQGRGYETSDSRFPE
GETRPVGRLQLDASYSPIKRVSYTVENARVEQRTDLDKLVIDLETNGTVDPEEAIRKAATILQQQIAIFVDLQKDQAPVA
QEPREEVDPILLRPVDDLELTVRSANCLKAENIYYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASKGLQLGMRLENWP
PASLRMDDRFAYRSR
>Mature_334_residues
TRTANEFLTPQAIKVEAVSGTSAKVILEPLERGFGHTLGNALRRILLSSLPGAAVVEVEIEGVEHEYSTLEGLQQDIVEL
LLNLKGLSIKLFDQNEAYLTLEKQGPGDITAADLRLPHNVEVVNPEHLIGTLSASGSIKMRLKVSQGRGYETSDSRFPEG
ETRPVGRLQLDASYSPIKRVSYTVENARVEQRTDLDKLVIDLETNGTVDPEEAIRKAATILQQQIAIFVDLQKDQAPVAQ
EPREEVDPILLRPVDDLELTVRSANCLKAENIYYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASKGLQLGMRLENWPP
ASLRMDDRFAYRSR

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=326, Percent_Identity=66.8711656441718, Blast_Score=438, Evalue=1e-124,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_ACIAD (Q6F7T7)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047700.1
- ProteinModelPortal:   Q6F7T7
- STRING:   Q6F7T7
- GeneID:   2879828
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3194
- NMPDR:   fig|62977.3.peg.2893
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   Q6F7T7
- ProtClustDB:   PRK05182
- BioCyc:   ASP62977:ACIAD3194-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37217; Mature: 37086

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTANEFLTPQAIKVEAVSGTSAKVILEPLERGFGHTLGNALRRILLSSLPGAAVVEVE
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEE
IEGVEHEYSTLEGLQQDIVELLLNLKGLSIKLFDQNEAYLTLEKQGPGDITAADLRLPHN
EECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCCEEEEEEECCCC
VEVVNPEHLIGTLSASGSIKMRLKVSQGRGYETSDSRFPEGETRPVGRLQLDASYSPIKR
EEEECHHHEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHH
VSYTVENARVEQRTDLDKLVIDLETNGTVDPEEAIRKAATILQQQIAIFVDLQKDQAPVA
HEEEHHHCHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCC
QEPREEVDPILLRPVDDLELTVRSANCLKAENIYYIGDLVQRTEVELLKTPNLGKKSLTE
CCCHHHCCCEEECCCCCCEEEEECCCCEEECCEEEEHHHHHHHHHHEECCCCCCHHHHHH
IKDVLASKGLQLGMRLENWPPASLRMDDRFAYRSR
HHHHHHHCCCEECEEECCCCCCCCEECCCHHCCCC
>Mature Secondary Structure 
TRTANEFLTPQAIKVEAVSGTSAKVILEPLERGFGHTLGNALRRILLSSLPGAAVVEVE
CCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEE
IEGVEHEYSTLEGLQQDIVELLLNLKGLSIKLFDQNEAYLTLEKQGPGDITAADLRLPHN
EECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCCEEEEEEECCCC
VEVVNPEHLIGTLSASGSIKMRLKVSQGRGYETSDSRFPEGETRPVGRLQLDASYSPIKR
EEEECHHHEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHH
VSYTVENARVEQRTDLDKLVIDLETNGTVDPEEAIRKAATILQQQIAIFVDLQKDQAPVA
HEEEHHHCHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCC
QEPREEVDPILLRPVDDLELTVRSANCLKAENIYYIGDLVQRTEVELLKTPNLGKKSLTE
CCCHHHCCCEEECCCCCCEEEEECCCCEEECCEEEEHHHHHHHHHHEECCCCCCHHHHHH
IKDVLASKGLQLGMRLENWPPASLRMDDRFAYRSR
HHHHHHHCCCEECEEECCCCCCCCEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA