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The map label for this gene is rhtB [C]

Identifier: 50086167

GI number: 50086167

Start: 3094347

End: 3094982

Strand: Reverse

Name: rhtB [C]

Synonym: ACIAD3166

Alternate gene names: 50086167

Gene position: 3094982-3094347 (Counterclockwise)

Preceding gene: 50086168

Following gene: 50086162

Centisome position: 86.0

GC content: 34.75

Gene sequence:

>636_bases
ATGTCTGGACGTTTTATGAATTATTTCGAATTTGGGATATATGTATTTAGTATCATCATCATGATTGCAACACCTGGACC
TGTCATGATTTTGGTTGCAAGTGTTGGGTTGCAGCATGGTTACCAAGCTGCTTTCAAAACCATTGTAGGAACAAACCTGG
CTTCATTGTTTCTGATTGGAATCTCGATCTTGCTTCTAAAAGGGATGTTGAGTATCAATGCTTATATTTTTGCGGTCATC
AAAATCATGGGGTGCTTATATATTGCTTATCTAGGTTATGGGATTTTAAAAGAAGTTTCTGTATCTCATGCTCAGCCATT
AAAACTCACCCAGTTCAATGGTCGAAATGATTTTAAAAGAGGATTTTTGATCGCGATTTCAAATCCTAAAGATATTATTT
TCTTTTCTTCATTTTTTCCGCAGTTTATTGGCATACATCAGAATATCAATTTAAGTTTGATTATTTTGGTTAGTACATGG
ATTGTCTTAGATTTTTTAAAACTTTCAATGGTTTATCTTGGGTTTAATCGTATCGCAAAGGCAAAGTGGTATGCTCACAT
ATTGGGGGTATGTGGTGGAGTATTGATCATGATCGCATTATATGGACTCTGGGTTTTTATTTCGCCGTTGATTTAA

Upstream 100 bases:

>100_bases
CAAAAATGGTCAGAAAATTATCTTCGCTATAACATCGAATATCTATGTAACGGTTGTTGCTTATAAAAAATAGAACATAG
GCTTCTTTGAGTGGCAGCCT

Downstream 100 bases:

>100_bases
TTCTAGGAGCTTATTTTAAATAAGATCCTAGAAAGAATATTAAATTGAGCTTTGCCCTTAGAGCGTTTGATTAAAACGAT
AGCCACAATGCGTGCAACGA

Product: amino-acid efflux transmembrane protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MSGRFMNYFEFGIYVFSIIIMIATPGPVMILVASVGLQHGYQAAFKTIVGTNLASLFLIGISILLLKGMLSINAYIFAVI
KIMGCLYIAYLGYGILKEVSVSHAQPLKLTQFNGRNDFKRGFLIAISNPKDIIFFSSFFPQFIGIHQNINLSLIILVSTW
IVLDFLKLSMVYLGFNRIAKAKWYAHILGVCGGVLIMIALYGLWVFISPLI

Sequences:

>Translated_211_residues
MSGRFMNYFEFGIYVFSIIIMIATPGPVMILVASVGLQHGYQAAFKTIVGTNLASLFLIGISILLLKGMLSINAYIFAVI
KIMGCLYIAYLGYGILKEVSVSHAQPLKLTQFNGRNDFKRGFLIAISNPKDIIFFSSFFPQFIGIHQNINLSLIILVSTW
IVLDFLKLSMVYLGFNRIAKAKWYAHILGVCGGVLIMIALYGLWVFISPLI
>Mature_210_residues
SGRFMNYFEFGIYVFSIIIMIATPGPVMILVASVGLQHGYQAAFKTIVGTNLASLFLIGISILLLKGMLSINAYIFAVIK
IMGCLYIAYLGYGILKEVSVSHAQPLKLTQFNGRNDFKRGFLIAISNPKDIIFFSSFFPQFIGIHQNINLSLIILVSTWI
VLDFLKLSMVYLGFNRIAKAKWYAHILGVCGGVLIMIALYGLWVFISPLI

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=170, Percent_Identity=31.1764705882353, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI48994978, Length=176, Percent_Identity=28.4090909090909, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23506; Mature: 23375

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRFMNYFEFGIYVFSIIIMIATPGPVMILVASVGLQHGYQAAFKTIVGTNLASLFLIG
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISILLLKGMLSINAYIFAVIKIMGCLYIAYLGYGILKEVSVSHAQPLKLTQFNGRNDFKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
GFLIAISNPKDIIFFSSFFPQFIGIHQNINLSLIILVSTWIVLDFLKLSMVYLGFNRIAK
CEEEEECCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKWYAHILGVCGGVLIMIALYGLWVFISPLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGRFMNYFEFGIYVFSIIIMIATPGPVMILVASVGLQHGYQAAFKTIVGTNLASLFLIG
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISILLLKGMLSINAYIFAVIKIMGCLYIAYLGYGILKEVSVSHAQPLKLTQFNGRNDFKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
GFLIAISNPKDIIFFSSFFPQFIGIHQNINLSLIILVSTWIVLDFLKLSMVYLGFNRIAK
CEEEEECCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKWYAHILGVCGGVLIMIALYGLWVFISPLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8169201; 10984043 [H]