Click here to switch to the map view.

The map label for this gene is miaB

Identifier: 50086159

GI number: 50086159

Start: 3085632

End: 3087083

Strand: Direct

Name: miaB

Synonym: ACIAD3158

Alternate gene names: 50086159

Gene position: 3085632-3087083 (Clockwise)

Preceding gene: 50086154

Following gene: 50086160

Centisome position: 85.74

GC content: 41.6

Gene sequence:

>1452_bases
ATGACGGTTCAAACCTTCATCCCGAATGGTGCCAAAGCTGCCTCAGAAAACACTGTCAACCAGCCTGTGCACACCTCTGA
TGTTTCAATCAAGAAATTGTATATTGAAACTCAAGGTTGCCAGATGAATGAGTATGACAGTCATCGTATGGCAGATCTTT
TAGGCGATTCACATGGTTATGTGCTCACCACAGACCCAAAAGAAGCAGATATTCTGCTCATGAATACCTGTTCGATTCGC
GAAAAAGCACAGGAAAAAGTATTTAGTGAGTTAGGTCGCTGGCGTAAGCTGAAACAGCAAAATCCTGACCTGATTATTGG
CGTAGGTGGCTGTGTTGCTTCACAAGAAGGTGACAATATTCAAAAACGTGCGCCTTATGTTGATATGGTGTTTGGCCCGC
AGACCTTGCATCGTCTGCCGCAAATGTTAGATCAACATCAAGATCAGATTGAAAAACCGAAAAAAGACAAAATCAAATTG
GTCGATATTTCTTTTCCAGATATCGAGAAGTTTGACTTTTTACCAGAACCGCGTGTTGAAGGGTTTAAAGCTTTTGTTTC
AATCATGGAAGGCTGCTCAAAATACTGTTCATTCTGTGTCGTTCCTTATACCCGTGGCGAAGAAGTATCACGTCCTTTAG
ATGACGTACTTGCAGAAATTGCTGGACTTGCAGAAAAAGGGGTACGTGAAATTTCACTGCTTGGACAAAATGTAAACGGA
TATCGTGGTGAAACTTTTGAAGGGGGTATTTGTACCTTCCCAGAATTGTTACGACTCGTTTCAGAAATTCCAGGGATTGG
ACGTTTACGCTATACCACTTCACATCCGCTTGAGTTTTCTGAAGAGCTGATTCAATGCTATCGAGACTTACCACAGATGG
TCTCTCATCTGCACTTGCCTGTGCAAAGTGGTTCAAATGCTGTGCTTCAAGCCATGAAGCGTAACCATACTATTGATGTA
TATATTGAGAAGATTGCCAAGCTACGTAAAATCCGTCCAGATATGCATTTATCTAGCGATTTTATTATTGGTTTTCCAGG
GGAAACAGAGCAAAACTTCGAAGAAACTTATCAATTTATCAAAGACTTGGATTTCGATCACTCTTATAGTTTTATTTATT
CAAAGCGCCCAGGTACGCCTGCGTCAGAGCTTGAAGACACCACCTCTGAAGCGGTCAAAAAAGAACGTTTGGCCAAAGTT
CAACACTGGATTAAACAATCGAGTATCCGTAAAACAGATGCCATGCAAGGTACCATTCAGCGTGTTTTGATTGAAAACGT
TTCAGAAAAAGATCCAAATCTTTTGGTGGGTACAGCAGACAATACACGTCTGGTTACATTTGTAGGAGACCCAATGTGGG
TGGGCCGCTTTGCAGAAATTGAGATTACCGAGATCAAAACTTTGAATTTAGTTTATGGTGAACTCTTGAATCTTGAGCCT
GACGTGGCGTAA

Upstream 100 bases:

>100_bases
TTATTTATTTTGATATCTTTTTTGCAAATGCTGCTACAATTGCGCCCTTTAAAAGAGTGTACCCTATACACTTGTCGATC
CAGTTTGTTTAGGAGCCTAC

Downstream 100 bases:

>100_bases
TGTAAGGGTATTAGAACAAGGCTAGAAAAAGGATAACTCTTTGACTGCAGCGATTCGACGTACCGTTACTTTTCCCGGCG
TTTCAATGGCTCGTTTAAAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTVQTFIPNGAKAASENTVNQPVHTSDVSIKKLYIETQGCQMNEYDSHRMADLLGDSHGYVLTTDPKEADILLMNTCSIR
EKAQEKVFSELGRWRKLKQQNPDLIIGVGGCVASQEGDNIQKRAPYVDMVFGPQTLHRLPQMLDQHQDQIEKPKKDKIKL
VDISFPDIEKFDFLPEPRVEGFKAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDVLAEIAGLAEKGVREISLLGQNVNG
YRGETFEGGICTFPELLRLVSEIPGIGRLRYTTSHPLEFSEELIQCYRDLPQMVSHLHLPVQSGSNAVLQAMKRNHTIDV
YIEKIAKLRKIRPDMHLSSDFIIGFPGETEQNFEETYQFIKDLDFDHSYSFIYSKRPGTPASELEDTTSEAVKKERLAKV
QHWIKQSSIRKTDAMQGTIQRVLIENVSEKDPNLLVGTADNTRLVTFVGDPMWVGRFAEIEITEIKTLNLVYGELLNLEP
DVA

Sequences:

>Translated_483_residues
MTVQTFIPNGAKAASENTVNQPVHTSDVSIKKLYIETQGCQMNEYDSHRMADLLGDSHGYVLTTDPKEADILLMNTCSIR
EKAQEKVFSELGRWRKLKQQNPDLIIGVGGCVASQEGDNIQKRAPYVDMVFGPQTLHRLPQMLDQHQDQIEKPKKDKIKL
VDISFPDIEKFDFLPEPRVEGFKAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDVLAEIAGLAEKGVREISLLGQNVNG
YRGETFEGGICTFPELLRLVSEIPGIGRLRYTTSHPLEFSEELIQCYRDLPQMVSHLHLPVQSGSNAVLQAMKRNHTIDV
YIEKIAKLRKIRPDMHLSSDFIIGFPGETEQNFEETYQFIKDLDFDHSYSFIYSKRPGTPASELEDTTSEAVKKERLAKV
QHWIKQSSIRKTDAMQGTIQRVLIENVSEKDPNLLVGTADNTRLVTFVGDPMWVGRFAEIEITEIKTLNLVYGELLNLEP
DVA
>Mature_482_residues
TVQTFIPNGAKAASENTVNQPVHTSDVSIKKLYIETQGCQMNEYDSHRMADLLGDSHGYVLTTDPKEADILLMNTCSIRE
KAQEKVFSELGRWRKLKQQNPDLIIGVGGCVASQEGDNIQKRAPYVDMVFGPQTLHRLPQMLDQHQDQIEKPKKDKIKLV
DISFPDIEKFDFLPEPRVEGFKAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDVLAEIAGLAEKGVREISLLGQNVNGY
RGETFEGGICTFPELLRLVSEIPGIGRLRYTTSHPLEFSEELIQCYRDLPQMVSHLHLPVQSGSNAVLQAMKRNHTIDVY
IEKIAKLRKIRPDMHLSSDFIIGFPGETEQNFEETYQFIKDLDFDHSYSFIYSKRPGTPASELEDTTSEAVKKERLAKVQ
HWIKQSSIRKTDAMQGTIQRVLIENVSEKDPNLLVGTADNTRLVTFVGDPMWVGRFAEIEITEIKTLNLVYGELLNLEPD
VA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=445, Percent_Identity=34.1573033707865, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI28872782, Length=445, Percent_Identity=34.1573033707865, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI93277076, Length=468, Percent_Identity=27.991452991453, Blast_Score=169, Evalue=7e-42,
Organism=Escherichia coli, GI1786882, Length=453, Percent_Identity=58.0573951434879, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1787057, Length=410, Percent_Identity=28.780487804878, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=466, Percent_Identity=31.3304721030043, Blast_Score=231, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI71996771, Length=327, Percent_Identity=28.4403669724771, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356207, Length=437, Percent_Identity=33.6384439359268, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI19922432, Length=479, Percent_Identity=28.39248434238, Blast_Score=141, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_ACIAD (Q6F7W8)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047669.1
- ProteinModelPortal:   Q6F7W8
- SMR:   Q6F7W8
- STRING:   Q6F7W8
- GeneID:   2878159
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3158
- NMPDR:   fig|62977.3.peg.2862
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q6F7W8
- ProtClustDB:   PRK14325
- BioCyc:   ASP62977:ACIAD3158-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 54648; Mature: 54517

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQTFIPNGAKAASENTVNQPVHTSDVSIKKLYIETQGCQMNEYDSHRMADLLGDSHGY
CCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCCCCE
VLTTDPKEADILLMNTCSIREKAQEKVFSELGRWRKLKQQNPDLIIGVGGCVASQEGDNI
EEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCH
QKRAPYVDMVFGPQTLHRLPQMLDQHQDQIEKPKKDKIKLVDISFPDIEKFDFLPEPRVE
HHHCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCCCCCCHHH
GFKAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDVLAEIAGLAEKGVREISLLGQNVNG
HHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YRGETFEGGICTFPELLRLVSEIPGIGRLRYTTSHPLEFSEELIQCYRDLPQMVSHLHLP
CCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC
VQSGSNAVLQAMKRNHTIDVYIEKIAKLRKIRPDMHLSSDFIIGFPGETEQNFEETYQFI
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHH
KDLDFDHSYSFIYSKRPGTPASELEDTTSEAVKKERLAKVQHWIKQSSIRKTDAMQGTIQ
HHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLIENVSEKDPNLLVGTADNTRLVTFVGDPMWVGRFAEIEITEIKTLNLVYGELLNLEP
HHHHHHCCCCCCCEEEECCCCCEEEEEECCCHHCCEEEEEEEEEEEEHHHHHHHHHCCCC
DVA
CCC
>Mature Secondary Structure 
TVQTFIPNGAKAASENTVNQPVHTSDVSIKKLYIETQGCQMNEYDSHRMADLLGDSHGY
CEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCCCCE
VLTTDPKEADILLMNTCSIREKAQEKVFSELGRWRKLKQQNPDLIIGVGGCVASQEGDNI
EEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCH
QKRAPYVDMVFGPQTLHRLPQMLDQHQDQIEKPKKDKIKLVDISFPDIEKFDFLPEPRVE
HHHCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCCCCCCHHH
GFKAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDVLAEIAGLAEKGVREISLLGQNVNG
HHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YRGETFEGGICTFPELLRLVSEIPGIGRLRYTTSHPLEFSEELIQCYRDLPQMVSHLHLP
CCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC
VQSGSNAVLQAMKRNHTIDVYIEKIAKLRKIRPDMHLSSDFIIGFPGETEQNFEETYQFI
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHH
KDLDFDHSYSFIYSKRPGTPASELEDTTSEAVKKERLAKVQHWIKQSSIRKTDAMQGTIQ
HHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLIENVSEKDPNLLVGTADNTRLVTFVGDPMWVGRFAEIEITEIKTLNLVYGELLNLEP
HHHHHHCCCCCCCEEEECCCCCEEEEEECCCHHCCEEEEEEEEEEEEHHHHHHHHHCCCC
DVA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA