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The map label for this gene is pdhD [H]

Identifier: 50086148

GI number: 50086148

Start: 3068356

End: 3069744

Strand: Direct

Name: pdhD [H]

Synonym: ACIAD3145

Alternate gene names: 50086148

Gene position: 3068356-3069744 (Clockwise)

Preceding gene: 50086146

Following gene: 50086149

Centisome position: 85.26

GC content: 38.3

Gene sequence:

>1389_bases
ATGTACGACATTATTATTATTGGTGCAGGAACAGCAGGTATTAGTGCCTACAAAGAAGCGGTCAAATATACCCAAAATAT
TCTGATTATGAACGATGGACCTTGGGATACCACTTGTGCACGTGTAGGATGCATGCCAAGTAAAGTTCTTATTTCATCCG
CCAACAGATTTCATGATCTGACACATCTTGAAGAGGTCGGATTAAAAGCTCACATAGAAAGAGAAATCGATACTTCTAAC
GTCATGTCGCATGTGCGTCAGTTGCGTGATGAATTTACTGCTGCAACCCTTAAAGACGTCAACCATTGGCCAAAACAACG
TAAAATTTCTGGAAAAGCAGTTTTTATTCATCCTGATACTGTTCAAATCAATAATCAACAGTATCAGGCACGTTCTTTTA
TATTGGCTGTAGGTTCAACACCAAGTGTTCCCGCGGAACTTAAACAAAGACTTAAAGATCGATTAATTACCTCCGATGAC
ATCTTTGAGTTACCCACTCTTCCTGAGTCAGTTGCTGTCATTGGTAGCGGTGTAATCGCGATAGAGCTTGCTCAGGCCAT
GTCCAGATTAGGCGTTAAAACCACGATTTTTGCTCGAAGTAAACGAATAGGCACATTAACTAGTCCAGCTTTACAGGAGC
TCGCTCAACAAATTTTAAGTGCAGAATTAGACATCAAATTTGAAATACTGCCAGATGATATGTCTCTAGATCAAAATAAA
GTAAAAGTTTCATATACAGAAAATGGAAAAAAGCGAACACAGCATTTTGATTATTTACTGCATGCAACAGGCCGAGATTC
GTTGCTTGCTACACTCGATCTAGAACAAATTTCATCTCAATTTTCTGATGTAAAAAAACTTCCAATAGATCCAGACACGA
AACAATTGGCAAATCTTCCAATTTTCATTGCAGGTGATGCACATACGACTACGCCAGTACAACATGAAGCGGCCTATGAA
GGTAAAATGCTGGTGCACAATTGTCTAAATTTTCCTAAAATTGAAAATGTCAAAACATTAACCCCATTAGGTATCGTTTT
TTGCTCCCCTGAAATGGCAATTGCAGGCAAATCATATAAACAATTGAAAGAACACGACATCGATTTTATTACAGGTTACG
TTTCCTATGAAAAACAGGGACGCGCCAAAGTTTTAGGGAAAAATAAAGGGGCAACTGAAATCTATATCGATCCCAAAAGC
CGACAACTTTTAGGTGCAGAAATTTTGGTAGAACAAGGTGAACATCTTGCACATCTATTAGTTTGGATGATTTCAGAACA
AAGTACAATTGATACCATATTAAATCACCCTTTCTATCATCCAACCCTTGAAGAAGGATTACGTACTGCCTTAAAGCATG
CCCGACGTCAAATTGACCAAATAGTATAA

Upstream 100 bases:

>100_bases
AGATTTTTGTATTTATACAAAATGAATAAAATCATATCACCTCATTGATGTAAATATTAATCATCTTATTGCAACTATAT
TTTTTATAAAAAGAGATGAC

Downstream 100 bases:

>100_bases
TTTGATGCTGATATTTCATGACTTATTATGATTTTTGGAATACTTATTGCTTTATGATAACTCATATTACTTTTAAGTTT
ATCTTCAATAAAGGGCAAAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MYDIIIIGAGTAGISAYKEAVKYTQNILIMNDGPWDTTCARVGCMPSKVLISSANRFHDLTHLEEVGLKAHIEREIDTSN
VMSHVRQLRDEFTAATLKDVNHWPKQRKISGKAVFIHPDTVQINNQQYQARSFILAVGSTPSVPAELKQRLKDRLITSDD
IFELPTLPESVAVIGSGVIAIELAQAMSRLGVKTTIFARSKRIGTLTSPALQELAQQILSAELDIKFEILPDDMSLDQNK
VKVSYTENGKKRTQHFDYLLHATGRDSLLATLDLEQISSQFSDVKKLPIDPDTKQLANLPIFIAGDAHTTTPVQHEAAYE
GKMLVHNCLNFPKIENVKTLTPLGIVFCSPEMAIAGKSYKQLKEHDIDFITGYVSYEKQGRAKVLGKNKGATEIYIDPKS
RQLLGAEILVEQGEHLAHLLVWMISEQSTIDTILNHPFYHPTLEEGLRTALKHARRQIDQIV

Sequences:

>Translated_462_residues
MYDIIIIGAGTAGISAYKEAVKYTQNILIMNDGPWDTTCARVGCMPSKVLISSANRFHDLTHLEEVGLKAHIEREIDTSN
VMSHVRQLRDEFTAATLKDVNHWPKQRKISGKAVFIHPDTVQINNQQYQARSFILAVGSTPSVPAELKQRLKDRLITSDD
IFELPTLPESVAVIGSGVIAIELAQAMSRLGVKTTIFARSKRIGTLTSPALQELAQQILSAELDIKFEILPDDMSLDQNK
VKVSYTENGKKRTQHFDYLLHATGRDSLLATLDLEQISSQFSDVKKLPIDPDTKQLANLPIFIAGDAHTTTPVQHEAAYE
GKMLVHNCLNFPKIENVKTLTPLGIVFCSPEMAIAGKSYKQLKEHDIDFITGYVSYEKQGRAKVLGKNKGATEIYIDPKS
RQLLGAEILVEQGEHLAHLLVWMISEQSTIDTILNHPFYHPTLEEGLRTALKHARRQIDQIV
>Mature_462_residues
MYDIIIIGAGTAGISAYKEAVKYTQNILIMNDGPWDTTCARVGCMPSKVLISSANRFHDLTHLEEVGLKAHIEREIDTSN
VMSHVRQLRDEFTAATLKDVNHWPKQRKISGKAVFIHPDTVQINNQQYQARSFILAVGSTPSVPAELKQRLKDRLITSDD
IFELPTLPESVAVIGSGVIAIELAQAMSRLGVKTTIFARSKRIGTLTSPALQELAQQILSAELDIKFEILPDDMSLDQNK
VKVSYTENGKKRTQHFDYLLHATGRDSLLATLDLEQISSQFSDVKKLPIDPDTKQLANLPIFIAGDAHTTTPVQHEAAYE
GKMLVHNCLNFPKIENVKTLTPLGIVFCSPEMAIAGKSYKQLKEHDIDFITGYVSYEKQGRAKVLGKNKGATEIYIDPKS
RQLLGAEILVEQGEHLAHLLVWMISEQSTIDTILNHPFYHPTLEEGLRTALKHARRQIDQIV

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=471, Percent_Identity=24.6284501061571, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI291045266, Length=351, Percent_Identity=24.7863247863248, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI50301238, Length=401, Percent_Identity=24.1895261845387, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI291045268, Length=320, Percent_Identity=25.625, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI33519430, Length=404, Percent_Identity=24.2574257425743, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI33519428, Length=404, Percent_Identity=24.2574257425743, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI33519426, Length=404, Percent_Identity=24.2574257425743, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI148277065, Length=404, Percent_Identity=24.2574257425743, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI148277071, Length=404, Percent_Identity=24.2574257425743, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786307, Length=463, Percent_Identity=23.5421166306695, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082354, Length=472, Percent_Identity=23.0932203389831, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789915, Length=352, Percent_Identity=25, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081717, Length=464, Percent_Identity=21.1206896551724, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=470, Percent_Identity=25.3191489361702, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17557007, Length=304, Percent_Identity=25, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71983429, Length=366, Percent_Identity=25.1366120218579, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71983419, Length=366, Percent_Identity=25.1366120218579, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=26.0416666666667, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325240, Length=495, Percent_Identity=22.2222222222222, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325166, Length=380, Percent_Identity=23.6842105263158, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21358499, Length=467, Percent_Identity=25.4817987152034, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640553, Length=495, Percent_Identity=24.6464646464646, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24640549, Length=495, Percent_Identity=24.6464646464646, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24640551, Length=495, Percent_Identity=24.6464646464646, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI17737741, Length=343, Percent_Identity=25.0728862973761, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51550; Mature: 51550

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDIIIIGAGTAGISAYKEAVKYTQNILIMNDGPWDTTCARVGCMPSKVLISSANRFHDL
CEEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHHCCCCHHHHHHCCHHHHHH
THLEEVGLKAHIEREIDTSNVMSHVRQLRDEFTAATLKDVNHWPKQRKISGKAVFIHPDT
HHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCE
VQINNQQYQARSFILAVGSTPSVPAELKQRLKDRLITSDDIFELPTLPESVAVIGSGVIA
EEECCCCEEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHHCCCHHH
IELAQAMSRLGVKTTIFARSKRIGTLTSPALQELAQQILSAELDIKFEILPDDMSLDQNK
HHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCE
VKVSYTENGKKRTQHFDYLLHATGRDSLLATLDLEQISSQFSDVKKLPIDPDTKQLANLP
EEEEECCCCHHHHHHHHHHEECCCCCCEEEEECHHHHHHHHHHHHCCCCCCCHHHHCCCC
IFIAGDAHTTTPVQHEAAYEGKMLVHNCLNFPKIENVKTLTPLGIVFCSPEMAIAGKSYK
EEEECCCCCCCCCCHHHHCCCHHHHHHHCCCCCCCCCCEECCCEEEEECCCCEECCCHHH
QLKEHDIDFITGYVSYEKQGRAKVLGKNKGATEIYIDPKSRQLLGAEILVEQGEHLAHLL
HHHHCCCCEEEEEEEECCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHH
VWMISEQSTIDTILNHPFYHPTLEEGLRTALKHARRQIDQIV
HHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYDIIIIGAGTAGISAYKEAVKYTQNILIMNDGPWDTTCARVGCMPSKVLISSANRFHDL
CEEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHHCCCCHHHHHHCCHHHHHH
THLEEVGLKAHIEREIDTSNVMSHVRQLRDEFTAATLKDVNHWPKQRKISGKAVFIHPDT
HHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCE
VQINNQQYQARSFILAVGSTPSVPAELKQRLKDRLITSDDIFELPTLPESVAVIGSGVIA
EEECCCCEEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHHCCCHHH
IELAQAMSRLGVKTTIFARSKRIGTLTSPALQELAQQILSAELDIKFEILPDDMSLDQNK
HHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCE
VKVSYTENGKKRTQHFDYLLHATGRDSLLATLDLEQISSQFSDVKKLPIDPDTKQLANLP
EEEEECCCCHHHHHHHHHHEECCCCCCEEEEECHHHHHHHHHHHHCCCCCCCHHHHCCCC
IFIAGDAHTTTPVQHEAAYEGKMLVHNCLNFPKIENVKTLTPLGIVFCSPEMAIAGKSYK
EEEECCCCCCCCCCHHHHCCCHHHHHHHCCCCCCCCCCEECCCEEEEECCCCEECCCHHH
QLKEHDIDFITGYVSYEKQGRAKVLGKNKGATEIYIDPKSRQLLGAEILVEQGEHLAHLL
HHHHCCCCEEEEEEEECCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHH
VWMISEQSTIDTILNHPFYHPTLEEGLRTALKHARRQIDQIV
HHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]