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The map label for this gene is fabI [H]

Identifier: 50086120

GI number: 50086120

Start: 3046323

End: 3047198

Strand: Reverse

Name: fabI [H]

Synonym: ACIAD3116

Alternate gene names: 50086120

Gene position: 3047198-3046323 (Counterclockwise)

Preceding gene: 50086121

Following gene: 50086119

Centisome position: 84.68

GC content: 43.15

Gene sequence:

>876_bases
ATGCTTTATGCAATTTCGCAAAGTGTCGAAGCTTGGCATTATGCTTCTAAATTTATTAAAGTGAGAAACCCAATGGCACA
AGGACTATTAACAGGTAAGCGCTTTTTAATTGCAGGTGTTGCTAGTAAGTTATCAATTGCATACGGTATTGCTCAGGCAT
TACATCGCGAAGGTGCAGAGCTTGCATTTACTTATCCAAATGAAAAACTAAAAAAGCGTGTTGATGAATTTGCTGCACAA
TTTGATTCAACGCTGGTTTTTCCTTGTGATGTTGCTGTTGATGCAGAAATTGACAATGCTTTTGTAGAACTCGCAAAACA
TTGGGATGGTGTGGATGGTGTGGTTCATTCGATTGGTTTTGCACCAGCGCATACACTGGACGGCGATTTTACGGACGTGA
CAGATCGTGAAGGTTTCCGTATTGCACACGATATCAGTGCCTATAGCTTTGTCGCAATGGCACGTGCAGCGAAGCCATTG
CTTCAAGCGCGTCAAGGCTGCTTGTTGACGCTGACTTATCAGGGTTCTGAGCGTGTGATGCCAAATTACAACGTGATGGG
ACTTGCCAAGGCATCTTTAGAAGCTGGTGTTCGTTATTTAGCATCTAGTCTTGGAGCAGATGGAATTCGTGTTAATGCGA
TTTCTGCGGGGCCAATTCGTACCTTGGCGGCCTCAGGTATTAAATCGTTCCGTAAAATGCTCGATGTCAACGAAAAAGTA
TCCCCGTTGAAACGTAACGTGACTATTGAAGAAGTGGGTAATGCTGCATTGTTCTTATGTTCACCTTGGGCTTCAGGTAT
TACGGGTGAAATCATGTATGTCGATGCGGGTTTTAATACCGTAGGAATGAGCCAAGCAATTATGGATGAAGAATAA

Upstream 100 bases:

>100_bases
GTTTGATCGGAATATTGTCAAACGACTAAAATGATCAGTCCCAAATAGCCTCAATCGCATTCTTTAGAGAGTGCGATTTT
TTTATGTCTATGCAAAAAAG

Downstream 100 bases:

>100_bases
TCATAATTCTGTCCATGAATAAAAAAATGATGCCTTTGAGCATCATTTTTTATAGTTTTAAGTATTTTTTAATCTACATA
TTTACTAAAAAACAAACAAC

Product: NADH-dependent enoyl-ACP reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLYAISQSVEAWHYASKFIKVRNPMAQGLLTGKRFLIAGVASKLSIAYGIAQALHREGAELAFTYPNEKLKKRVDEFAAQ
FDSTLVFPCDVAVDAEIDNAFVELAKHWDGVDGVVHSIGFAPAHTLDGDFTDVTDREGFRIAHDISAYSFVAMARAAKPL
LQARQGCLLTLTYQGSERVMPNYNVMGLAKASLEAGVRYLASSLGADGIRVNAISAGPIRTLAASGIKSFRKMLDVNEKV
SPLKRNVTIEEVGNAALFLCSPWASGITGEIMYVDAGFNTVGMSQAIMDEE

Sequences:

>Translated_291_residues
MLYAISQSVEAWHYASKFIKVRNPMAQGLLTGKRFLIAGVASKLSIAYGIAQALHREGAELAFTYPNEKLKKRVDEFAAQ
FDSTLVFPCDVAVDAEIDNAFVELAKHWDGVDGVVHSIGFAPAHTLDGDFTDVTDREGFRIAHDISAYSFVAMARAAKPL
LQARQGCLLTLTYQGSERVMPNYNVMGLAKASLEAGVRYLASSLGADGIRVNAISAGPIRTLAASGIKSFRKMLDVNEKV
SPLKRNVTIEEVGNAALFLCSPWASGITGEIMYVDAGFNTVGMSQAIMDEE
>Mature_291_residues
MLYAISQSVEAWHYASKFIKVRNPMAQGLLTGKRFLIAGVASKLSIAYGIAQALHREGAELAFTYPNEKLKKRVDEFAAQ
FDSTLVFPCDVAVDAEIDNAFVELAKHWDGVDGVVHSIGFAPAHTLDGDFTDVTDREGFRIAHDISAYSFVAMARAAKPL
LQARQGCLLTLTYQGSERVMPNYNVMGLAKASLEAGVRYLASSLGADGIRVNAISAGPIRTLAASGIKSFRKMLDVNEKV
SPLKRNVTIEEVGNAALFLCSPWASGITGEIMYVDAGFNTVGMSQAIMDEE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=65.5038759689922, Blast_Score=350, Evalue=7e-98,
Organism=Escherichia coli, GI1787905, Length=257, Percent_Identity=29.1828793774319, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=29.2307692307692, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=254, Percent_Identity=28.740157480315, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 31490; Mature: 31490

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYAISQSVEAWHYASKFIKVRNPMAQGLLTGKRFLIAGVASKLSIAYGIAQALHREGAE
CEEEECHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
LAFTYPNEKLKKRVDEFAAQFDSTLVFPCDVAVDAEIDNAFVELAKHWDGVDGVVHSIGF
EEEECCCHHHHHHHHHHHHHHCCEEEEEECEEEECHHHHHHHHHHHHCCCHHHHHHHHCC
APAHTLDGDFTDVTDREGFRIAHDISAYSFVAMARAAKPLLQARQGCLLTLTYQGSERVM
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
PNYNVMGLAKASLEAGVRYLASSLGADGIRVNAISAGPIRTLAASGIKSFRKMLDVNEKV
CCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHH
SPLKRNVTIEEVGNAALFLCSPWASGITGEIMYVDAGFNTVGMSQAIMDEE
HHHHHCCCHHHHCCEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHCCCC
>Mature Secondary Structure
MLYAISQSVEAWHYASKFIKVRNPMAQGLLTGKRFLIAGVASKLSIAYGIAQALHREGAE
CEEEECHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
LAFTYPNEKLKKRVDEFAAQFDSTLVFPCDVAVDAEIDNAFVELAKHWDGVDGVVHSIGF
EEEECCCHHHHHHHHHHHHHHCCEEEEEECEEEECHHHHHHHHHHHHCCCHHHHHHHHCC
APAHTLDGDFTDVTDREGFRIAHDISAYSFVAMARAAKPLLQARQGCLLTLTYQGSERVM
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
PNYNVMGLAKASLEAGVRYLASSLGADGIRVNAISAGPIRTLAASGIKSFRKMLDVNEKV
CCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHH
SPLKRNVTIEEVGNAALFLCSPWASGITGEIMYVDAGFNTVGMSQAIMDEE
HHHHHCCCHHHHCCEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10464225; 10984043 [H]