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The map label for this gene is holA [H]

Identifier: 50086112

GI number: 50086112

Start: 3032008

End: 3033003

Strand: Direct

Name: holA [H]

Synonym: ACIAD3108

Alternate gene names: 50086112

Gene position: 3032008-3033003 (Clockwise)

Preceding gene: 50086111

Following gene: 50086113

Centisome position: 84.25

GC content: 45.08

Gene sequence:

>996_bases
ATGAAACTTGATTATTTACAAGCACTCAAGCGGGTCGATGCGGCTCGTGGTGCTTGGATCATTCACGGTCAAGAGCCGTT
ACTCGAGCAAAACTTACTTGATGCCTTTCGCAAAAGCTGGCAGACGCAAGAGATCGAGCGGCAGCGTTATGATATTGGTA
GCGTCAGTGACTGGAAAAATGTATTTAATGGCTTAAATAGCCTGTCACTTTTTTCGCAGCAACTGGCGATTGAAGTGCAT
GGCAACATTAAGCCCGATGCTAGCGGAATCAAACAACTCAAAAGCTACTTGCAGCACAATGAACATAATTTGCTACTGGT
AGTCTTGCCTAAACAGGATGCCTCAAGTCTAAAGTCGTCTTTCTTTCAAACGGTTGAAGCCAATGGTGTAATTGCGCATT
TAAGCGCACAGTATCCGCAAGATCGTCAGCGTATTTTAGCGCAAGAAGCCGAAAAAATTGGGATTCAACTCGATCAGGAT
GCCTGGACATGGCTGATGCAGCACCACGAGCATAATCTGCTCGCGGCTAAAAATAGCCTCATGCGCGTGAGTGATACCTT
TCCCGAGCATCAACTGATTCAGATTGAACAGCTGTATGCCTGCCTACAAGATCAATCGCGCTATACCACCTATGACTTAA
GTGACGCCATGCTGCGTGGCAATCTGGCGCAATCGGTCAAAATTTTTCAGTACCTGATTGGTGCGGGAGAACCACACAGT
TTGATTTTGTGGACCTTAAGTAAAGAAATGCGTTTGCTGATGCAGCTCTTTGAACAACCTCAAAATGCATTGCAACTCGG
GATCTGGAAAACCAAGGTTTCAGACTATCAGCAAGCATTACGTCGTCTAAATCCACATCATTTTTTAAGCTGGCCTACAC
TTTTGTTGCGCATTGACAGTGCTATTAAAGGGATGTCGGATGAAAATCCCGAACACTTGATTTTACAAGCCTTAGCGGAA
TTATGTGGTACCTCGCTGTTTGCCCAAACATCCTAA

Upstream 100 bases:

>100_bases
CATATTATTGTGGATGATGTGGCGCAGCAAATTGCCCGTCAGATTTCGAATAACCGCTTACCAAAGCTCATCTCTCAGCC
TTAAGGGCTGTATCATTCGT

Downstream 100 bases:

>100_bases
TTTTCGCTTTTAATTCAACTAAATTAATAAAAATATTCACGTTTTATTCTCATTTCACTTCTATACTAGCGCCAGTCCTT
CTTACTTTGCCTGTATCACT

Product: DNA polymerase III subunit delta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKLDYLQALKRVDAARGAWIIHGQEPLLEQNLLDAFRKSWQTQEIERQRYDIGSVSDWKNVFNGLNSLSLFSQQLAIEVH
GNIKPDASGIKQLKSYLQHNEHNLLLVVLPKQDASSLKSSFFQTVEANGVIAHLSAQYPQDRQRILAQEAEKIGIQLDQD
AWTWLMQHHEHNLLAAKNSLMRVSDTFPEHQLIQIEQLYACLQDQSRYTTYDLSDAMLRGNLAQSVKIFQYLIGAGEPHS
LILWTLSKEMRLLMQLFEQPQNALQLGIWKTKVSDYQQALRRLNPHHFLSWPTLLLRIDSAIKGMSDENPEHLILQALAE
LCGTSLFAQTS

Sequences:

>Translated_331_residues
MKLDYLQALKRVDAARGAWIIHGQEPLLEQNLLDAFRKSWQTQEIERQRYDIGSVSDWKNVFNGLNSLSLFSQQLAIEVH
GNIKPDASGIKQLKSYLQHNEHNLLLVVLPKQDASSLKSSFFQTVEANGVIAHLSAQYPQDRQRILAQEAEKIGIQLDQD
AWTWLMQHHEHNLLAAKNSLMRVSDTFPEHQLIQIEQLYACLQDQSRYTTYDLSDAMLRGNLAQSVKIFQYLIGAGEPHS
LILWTLSKEMRLLMQLFEQPQNALQLGIWKTKVSDYQQALRRLNPHHFLSWPTLLLRIDSAIKGMSDENPEHLILQALAE
LCGTSLFAQTS
>Mature_331_residues
MKLDYLQALKRVDAARGAWIIHGQEPLLEQNLLDAFRKSWQTQEIERQRYDIGSVSDWKNVFNGLNSLSLFSQQLAIEVH
GNIKPDASGIKQLKSYLQHNEHNLLLVVLPKQDASSLKSSFFQTVEANGVIAHLSAQYPQDRQRILAQEAEKIGIQLDQD
AWTWLMQHHEHNLLAAKNSLMRVSDTFPEHQLIQIEQLYACLQDQSRYTTYDLSDAMLRGNLAQSVKIFQYLIGAGEPHS
LILWTLSKEMRLLMQLFEQPQNALQLGIWKTKVSDYQQALRRLNPHHFLSWPTLLLRIDSAIKGMSDENPEHLILQALAE
LCGTSLFAQTS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=280, Percent_Identity=26.7857142857143, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37947; Mature: 37947

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDYLQALKRVDAARGAWIIHGQEPLLEQNLLDAFRKSWQTQEIERQRYDIGSVSDWKN
CCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHH
VFNGLNSLSLFSQQLAIEVHGNIKPDASGIKQLKSYLQHNEHNLLLVVLPKQDASSLKSS
HHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
FFQTVEANGVIAHLSAQYPQDRQRILAQEAEKIGIQLDQDAWTWLMQHHEHNLLAAKNSL
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCEECHHHHHHHHHHHCCCHHHHHHHH
MRVSDTFPEHQLIQIEQLYACLQDQSRYTTYDLSDAMLRGNLAQSVKIFQYLIGAGEPHS
HHHHCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHCHHHHHHHHHHHHHCCCCCCE
LILWTLSKEMRLLMQLFEQPQNALQLGIWKTKVSDYQQALRRLNPHHFLSWPTLLLRIDS
EEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AIKGMSDENPEHLILQALAELCGTSLFAQTS
HHCCCCCCCHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MKLDYLQALKRVDAARGAWIIHGQEPLLEQNLLDAFRKSWQTQEIERQRYDIGSVSDWKN
CCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHH
VFNGLNSLSLFSQQLAIEVHGNIKPDASGIKQLKSYLQHNEHNLLLVVLPKQDASSLKSS
HHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
FFQTVEANGVIAHLSAQYPQDRQRILAQEAEKIGIQLDQDAWTWLMQHHEHNLLAAKNSL
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCEECHHHHHHHHHHHCCCHHHHHHHH
MRVSDTFPEHQLIQIEQLYACLQDQSRYTTYDLSDAMLRGNLAQSVKIFQYLIGAGEPHS
HHHHCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHCHHHHHHHHHHHHHCCCCCCE
LILWTLSKEMRLLMQLFEQPQNALQLGIWKTKVSDYQQALRRLNPHHFLSWPTLLLRIDS
EEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AIKGMSDENPEHLILQALAELCGTSLFAQTS
HHCCCCCCCHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]