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The map label for this gene is ilvI [H]

Identifier: 50086108

GI number: 50086108

Start: 3026035

End: 3027759

Strand: Reverse

Name: ilvI [H]

Synonym: ACIAD3104

Alternate gene names: 50086108

Gene position: 3027759-3026035 (Counterclockwise)

Preceding gene: 50086118

Following gene: 50086107

Centisome position: 84.14

GC content: 45.33

Gene sequence:

>1725_bases
TTGGAACTTTTATCTGGTGGTGAAATGCTCGTTCGCGCACTCGCGGATGAGGGCGTTGAGCACGTTTTTGGATACCCAGG
CGGCGCAGTTTTACATATTTATGATGCGCTTTTTCAACAAGACAAAATCAATCATTATCTTGTACGTCATGAGCAAGCTG
CTGGTCATATGGCTGATGCTTATTCACGTGTCACAGGTAAAACGGGTGTTGTGCTTGTTACGTCTGGCCCAGGGGCAACC
AATACCGTAACTCCAATTGCAACTGCATATATGGACTCGATTCCGATGGTCATCTTATCGGGTCAGGTTGCTTCACATCT
GATTGGTGAAGATGCCTTTCAGGAAACAGATATGGTCGGAATCTCCAGACCAATCGTAAAGCATAGTTTCCAAGTCCGCC
ATGCAAGCGAAATTCCTGCAATTATTAAAAAAGCATTTTATATCGCATCCTCTGGCCGTCCTGGCCCTGTCGTGATTGAT
ATTCCAAAGGATGCAACCAATCCAGCAGAAAAGTTTGCCTATGAATATCCAGAAAAGGTAAAACTACGCTCTTATCAACC
GCCTTCGCGTGGCCATTCGGGTCAAATTCGTAAGGCTGTCGACGAGCTTCTGACTGCAAAACGTCCAATGATCTATACGG
GCGGTGGTGTAGTGCAGGGTAATGCCTCTGAATTGCTGACTGAGCTTGCCCATCTGCTGGGTTTCCCTGTAACCAATACC
TTGATGGGACTGGGTGGCTTTCCAGGGGATGATCCGCAATTTGTAGGCATGCTTGGTATGCATGGTACTTATGAAGCCAA
TATGGCGATGCATAATGCTGATGTGATCTTGGCAATTGGTGCGCGTTTTGATGACCGTGTGACCAATAATCCTGCGAAAT
TTTGTCAAAATGCCAAAGTCATCCATGTGGATATCGATCCAGCTGCGATTTCTAAAACCATCATGGCGCATATCCCAATT
GTAGGTGCTGTTCAGCCTGTTCTTCAGGAAATGTTGACACAGTTAAAGCAATTGAATGTTTCTAAGCCAAATCCAGAAGC
GATTAGCGCGTGGTGGGATCAGATTAATGAATGGCGTAAAGTTCATGGTCTGCAATATCAAGCTGGTCAGGATGGCATTA
TGAAGCCTCAACAAGTGGTTGAAGCTTTATATAAAGCAACAGATGGCAATGCGATTATCACCTCGGATGTAGGGCAGCAT
CAAATGTTTGGCGCGCTGTATTACAAGTACAAGCGTCCGCGTCAGTGGATCAACTCGGGTGGTCTTGGCACCATGGGCGT
TGGTTTGCCGTATGCGATGGCAGCCAAACTGGCTTGTCCAGACCAACAAGTCGTCTGTATCACGGGTGAAGCCTCAATTC
AGATGTGTATTCAGGAATTATCAACCTGTAAGCAATATGGCTTAAACGTTAAGATTCTGTGCCTCAATAACCGTGCACTG
GGAATGGTCAAACAGTGGCAAGACATGAACTATGAAGGACGTCATTCAAGTTCTTATGTAGAATCATTGCCTGATTTTGC
CAAATTGATGGAAGCTTATGGCCATGTCGGAATCCAGATTGACCATACCGATGAGCTTGAGTCTAAACTCGCAGAAGCGA
TGGCCATTAATGATAAATGTGTATTTATCAATGTGATGGTTGATCGTACTGAACATGTGTATCCAATGTTGATTGCTGGG
CAATCGATGAAAGATATGTGGTTAGGGAAAGGGGAGCGTACATAA

Upstream 100 bases:

>100_bases
AAAAACTTGGAAATATGTGTGCTATAACAGTGCAGACAGTAAATGAATGAAGACTATTTATGCCTCCCATAAATAGTAAA
AAGACTTAGGGTGAATCACG

Downstream 100 bases:

>100_bases
TGAGACACATTATTTCTGTACTCGTTGAAAACGAAGCAGGTGCGCTTTCACGTTTGGTCGGATTATTTAGTCAACGTGGC
TACAACATTGAAACCTTGAA

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MELLSGGEMLVRALADEGVEHVFGYPGGAVLHIYDALFQQDKINHYLVRHEQAAGHMADAYSRVTGKTGVVLVTSGPGAT
NTVTPIATAYMDSIPMVILSGQVASHLIGEDAFQETDMVGISRPIVKHSFQVRHASEIPAIIKKAFYIASSGRPGPVVID
IPKDATNPAEKFAYEYPEKVKLRSYQPPSRGHSGQIRKAVDELLTAKRPMIYTGGGVVQGNASELLTELAHLLGFPVTNT
LMGLGGFPGDDPQFVGMLGMHGTYEANMAMHNADVILAIGARFDDRVTNNPAKFCQNAKVIHVDIDPAAISKTIMAHIPI
VGAVQPVLQEMLTQLKQLNVSKPNPEAISAWWDQINEWRKVHGLQYQAGQDGIMKPQQVVEALYKATDGNAIITSDVGQH
QMFGALYYKYKRPRQWINSGGLGTMGVGLPYAMAAKLACPDQQVVCITGEASIQMCIQELSTCKQYGLNVKILCLNNRAL
GMVKQWQDMNYEGRHSSSYVESLPDFAKLMEAYGHVGIQIDHTDELESKLAEAMAINDKCVFINVMVDRTEHVYPMLIAG
QSMKDMWLGKGERT

Sequences:

>Translated_574_residues
MELLSGGEMLVRALADEGVEHVFGYPGGAVLHIYDALFQQDKINHYLVRHEQAAGHMADAYSRVTGKTGVVLVTSGPGAT
NTVTPIATAYMDSIPMVILSGQVASHLIGEDAFQETDMVGISRPIVKHSFQVRHASEIPAIIKKAFYIASSGRPGPVVID
IPKDATNPAEKFAYEYPEKVKLRSYQPPSRGHSGQIRKAVDELLTAKRPMIYTGGGVVQGNASELLTELAHLLGFPVTNT
LMGLGGFPGDDPQFVGMLGMHGTYEANMAMHNADVILAIGARFDDRVTNNPAKFCQNAKVIHVDIDPAAISKTIMAHIPI
VGAVQPVLQEMLTQLKQLNVSKPNPEAISAWWDQINEWRKVHGLQYQAGQDGIMKPQQVVEALYKATDGNAIITSDVGQH
QMFGALYYKYKRPRQWINSGGLGTMGVGLPYAMAAKLACPDQQVVCITGEASIQMCIQELSTCKQYGLNVKILCLNNRAL
GMVKQWQDMNYEGRHSSSYVESLPDFAKLMEAYGHVGIQIDHTDELESKLAEAMAINDKCVFINVMVDRTEHVYPMLIAG
QSMKDMWLGKGERT
>Mature_574_residues
MELLSGGEMLVRALADEGVEHVFGYPGGAVLHIYDALFQQDKINHYLVRHEQAAGHMADAYSRVTGKTGVVLVTSGPGAT
NTVTPIATAYMDSIPMVILSGQVASHLIGEDAFQETDMVGISRPIVKHSFQVRHASEIPAIIKKAFYIASSGRPGPVVID
IPKDATNPAEKFAYEYPEKVKLRSYQPPSRGHSGQIRKAVDELLTAKRPMIYTGGGVVQGNASELLTELAHLLGFPVTNT
LMGLGGFPGDDPQFVGMLGMHGTYEANMAMHNADVILAIGARFDDRVTNNPAKFCQNAKVIHVDIDPAAISKTIMAHIPI
VGAVQPVLQEMLTQLKQLNVSKPNPEAISAWWDQINEWRKVHGLQYQAGQDGIMKPQQVVEALYKATDGNAIITSDVGQH
QMFGALYYKYKRPRQWINSGGLGTMGVGLPYAMAAKLACPDQQVVCITGEASIQMCIQELSTCKQYGLNVKILCLNNRAL
GMVKQWQDMNYEGRHSSSYVESLPDFAKLMEAYGHVGIQIDHTDELESKLAEAMAINDKCVFINVMVDRTEHVYPMLIAG
QSMKDMWLGKGERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=27.2566371681416, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI21361361, Length=593, Percent_Identity=24.283305227656, Blast_Score=147, Evalue=3e-35,
Organism=Escherichia coli, GI87081685, Length=576, Percent_Identity=61.6319444444444, Blast_Score=741, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=40.9574468085106, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1786717, Length=554, Percent_Identity=31.2274368231047, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=25.6272401433692, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1787096, Length=564, Percent_Identity=26.4184397163121, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17531299, Length=587, Percent_Identity=25.8943781942078, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17531301, Length=587, Percent_Identity=25.8943781942078, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=587, Percent_Identity=24.3611584327087, Blast_Score=144, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=584, Percent_Identity=43.1506849315069, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=489, Percent_Identity=24.9488752556237, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=26.3716814159292, Blast_Score=186, Evalue=5e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62840; Mature: 62840

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLSGGEMLVRALADEGVEHVFGYPGGAVLHIYDALFQQDKINHYLVRHEQAAGHMADA
CCCCCCHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHCCHHHHH
YSRVTGKTGVVLVTSGPGATNTVTPIATAYMDSIPMVILSGQVASHLIGEDAFQETDMVG
HHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHCCHHHHHCCCCC
ISRPIVKHSFQVRHASEIPAIIKKAFYIASSGRPGPVVIDIPKDATNPAEKFAYEYPEKV
CCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCCCCHHHHHHHCCCCCC
KLRSYQPPSRGHSGQIRKAVDELLTAKRPMIYTGGGVVQGNASELLTELAHLLGFPVTNT
EECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHHCCCHHHH
LMGLGGFPGDDPQFVGMLGMHGTYEANMAMHNADVILAIGARFDDRVTNNPAKFCQNAKV
HHHCCCCCCCCCCEEEEECCCCCEECCEEEECCCEEEEECCCCCCCCCCCHHHHHCCCEE
IHVDIDPAAISKTIMAHIPIVGAVQPVLQEMLTQLKQLNVSKPNPEAISAWWDQINEWRK
EEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
VHGLQYQAGQDGIMKPQQVVEALYKATDGNAIITSDVGQHQMFGALYYKYKRPRQWINSG
HCCCEECCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCHHHHHCCC
GLGTMGVGLPYAMAAKLACPDQQVVCITGEASIQMCIQELSTCKQYGLNVKILCLNNRAL
CCCCCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCH
GMVKQWQDMNYEGRHSSSYVESLPDFAKLMEAYGHVGIQIDHTDELESKLAEAMAINDKC
HHHHHHHCCCCCCCCCHHHHHHCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCE
VFINVMVDRTEHVYPMLIAGQSMKDMWLGKGERT
EEEEEEECCCCCEEEEEEECCCHHHHHCCCCCCC
>Mature Secondary Structure
MELLSGGEMLVRALADEGVEHVFGYPGGAVLHIYDALFQQDKINHYLVRHEQAAGHMADA
CCCCCCHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHCCHHHHH
YSRVTGKTGVVLVTSGPGATNTVTPIATAYMDSIPMVILSGQVASHLIGEDAFQETDMVG
HHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHCCHHHHHCCCCC
ISRPIVKHSFQVRHASEIPAIIKKAFYIASSGRPGPVVIDIPKDATNPAEKFAYEYPEKV
CCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCCCCHHHHHHHCCCCCC
KLRSYQPPSRGHSGQIRKAVDELLTAKRPMIYTGGGVVQGNASELLTELAHLLGFPVTNT
EECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHHCCCHHHH
LMGLGGFPGDDPQFVGMLGMHGTYEANMAMHNADVILAIGARFDDRVTNNPAKFCQNAKV
HHHCCCCCCCCCCEEEEECCCCCEECCEEEECCCEEEEECCCCCCCCCCCHHHHHCCCEE
IHVDIDPAAISKTIMAHIPIVGAVQPVLQEMLTQLKQLNVSKPNPEAISAWWDQINEWRK
EEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
VHGLQYQAGQDGIMKPQQVVEALYKATDGNAIITSDVGQHQMFGALYYKYKRPRQWINSG
HCCCEECCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCHHHHHCCC
GLGTMGVGLPYAMAAKLACPDQQVVCITGEASIQMCIQELSTCKQYGLNVKILCLNNRAL
CCCCCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCH
GMVKQWQDMNYEGRHSSSYVESLPDFAKLMEAYGHVGIQIDHTDELESKLAEAMAINDKC
HHHHHHHCCCCCCCCCHHHHHHCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCE
VFINVMVDRTEHVYPMLIAGQSMKDMWLGKGERT
EEEEEEECCCCCEEEEEEECCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]