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The map label for this gene is dusA [H]

Identifier: 50086096

GI number: 50086096

Start: 3011196

End: 3012221

Strand: Direct

Name: dusA [H]

Synonym: ACIAD3085

Alternate gene names: 50086096

Gene position: 3011196-3012221 (Clockwise)

Preceding gene: 50086095

Following gene: 50086097

Centisome position: 83.68

GC content: 43.18

Gene sequence:

>1026_bases
TTGTTTGGATCTGTTTTCATGAAATCATCACTAGAAACCTTAAAGCCGCGTATTTCGGTTGCCCCGATGATGGATTGGAC
GACCAAGGACTATCGCTTTTTTGCTCGACTGTTTAATCCCAATGTGGTGTTGTACACCGAAATGGTCACGACAGGCGCGA
TTTTATTTGGTGATACCAAACGCCATCTCGATTACAGCCATGGAGAACACCCTATTGTGCTACAACTGGGTGGCTCTAAT
CCCAAAGATCTGGCCGAATGTACCAAAATGGCACAGGACTGGGGCTATGATGAAGTCAATCTGAATGTTGGATGTCCAAG
TGACCGTGTACAAAACAATAAAATTGGTGCATGCCTGATGGCAGAACCTGACCTCGTGGCTGAATGTATTGCGAAAATGC
AACAGGTCGTTGATATTCCAGTACATGTCAAACATCGTATTGGCATTGATGACATGCAGTCTTATGACGAAATGCTGAAT
TTTGTAGATACTGTAGCAAAAACAGGCTGTCATCATTTTGTTGTCCATGCCAGAATTGCCTTGCTTAAAGGGTTATCACC
TAAAGAAAATCGTGAAATTCCGCCGTTACGCTATGAAGATGTGTACCGTCTGAAACAAGACCGTCCTGAACTGTTTATTG
AAATCAACGGTGGCATTAAAACAGTCGCAGAAACACAAGCACATTTACAACATGTCGATGGCGTGATGATTGGTCGCGAA
GCCTATCATAATCCGTATTTATTGGCAGAATTGGGGCAGCTCTGGAACTTGGACTTACCTGATCGTTTCAATATCATGCA
ACAAATGATGCCTTATATTGAACAACGTTTGGCTGAAGGTGCACCACTGTCGATTATTACGCGTCATATCTTAGGTCTGT
TTCAAAACTTGCCAGGTGCCCGTAAATGGCGACAAGCTCTCAGTGGGGGAAATGCAAAAACACGAAGCGATATTGAACAT
GCCTTATGCAATATTCATGAAGCAATTCAGCGAACAGAAGCATCTCTGCAAGTGCAAGATATTTGA

Upstream 100 bases:

>100_bases
CGGAATGAAAAACCCAATGTAGTGTGACAAACGTCCCCAATTGCATAATTTGGGGATTTAGCATAAACCGCGTATAATTT
ATGCCCTCGTTGCACACTCA

Downstream 100 bases:

>100_bases
AGAGACAAAGCCTTTATGATGCAATCATGAACATAAAGGTTTATTTTTTAATTGAGTCGCATATTGCAGAGCAGGTTTTA
ATTTTTACAATGATAGGAAT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MFGSVFMKSSLETLKPRISVAPMMDWTTKDYRFFARLFNPNVVLYTEMVTTGAILFGDTKRHLDYSHGEHPIVLQLGGSN
PKDLAECTKMAQDWGYDEVNLNVGCPSDRVQNNKIGACLMAEPDLVAECIAKMQQVVDIPVHVKHRIGIDDMQSYDEMLN
FVDTVAKTGCHHFVVHARIALLKGLSPKENREIPPLRYEDVYRLKQDRPELFIEINGGIKTVAETQAHLQHVDGVMIGRE
AYHNPYLLAELGQLWNLDLPDRFNIMQQMMPYIEQRLAEGAPLSIITRHILGLFQNLPGARKWRQALSGGNAKTRSDIEH
ALCNIHEAIQRTEASLQVQDI

Sequences:

>Translated_341_residues
MFGSVFMKSSLETLKPRISVAPMMDWTTKDYRFFARLFNPNVVLYTEMVTTGAILFGDTKRHLDYSHGEHPIVLQLGGSN
PKDLAECTKMAQDWGYDEVNLNVGCPSDRVQNNKIGACLMAEPDLVAECIAKMQQVVDIPVHVKHRIGIDDMQSYDEMLN
FVDTVAKTGCHHFVVHARIALLKGLSPKENREIPPLRYEDVYRLKQDRPELFIEINGGIKTVAETQAHLQHVDGVMIGRE
AYHNPYLLAELGQLWNLDLPDRFNIMQQMMPYIEQRLAEGAPLSIITRHILGLFQNLPGARKWRQALSGGNAKTRSDIEH
ALCNIHEAIQRTEASLQVQDI
>Mature_341_residues
MFGSVFMKSSLETLKPRISVAPMMDWTTKDYRFFARLFNPNVVLYTEMVTTGAILFGDTKRHLDYSHGEHPIVLQLGGSN
PKDLAECTKMAQDWGYDEVNLNVGCPSDRVQNNKIGACLMAEPDLVAECIAKMQQVVDIPVHVKHRIGIDDMQSYDEMLN
FVDTVAKTGCHHFVVHARIALLKGLSPKENREIPPLRYEDVYRLKQDRPELFIEINGGIKTVAETQAHLQHVDGVMIGRE
AYHNPYLLAELGQLWNLDLPDRFNIMQQMMPYIEQRLAEGAPLSIITRHILGLFQNLPGARKWRQALSGGNAKTRSDIEH
ALCNIHEAIQRTEASLQVQDI

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=304, Percent_Identity=22.0394736842105, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI40807366, Length=289, Percent_Identity=23.1833910034602, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI145693211, Length=313, Percent_Identity=56.5495207667732, Blast_Score=351, Evalue=4e-98,
Organism=Escherichia coli, GI1789660, Length=245, Percent_Identity=24.0816326530612, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788462, Length=180, Percent_Identity=30, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=313, Percent_Identity=26.1980830670927, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24580595, Length=320, Percent_Identity=24.6875, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920448, Length=320, Percent_Identity=24.6875, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38659; Mature: 38659

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGSVFMKSSLETLKPRISVAPMMDWTTKDYRFFARLFNPNVVLYTEMVTTGAILFGDTK
CCCHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHCCCEEEEEEHHHHCEEEECCCH
RHLDYSHGEHPIVLQLGGSNPKDLAECTKMAQDWGYDEVNLNVGCPSDRVQNNKIGACLM
HHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCCCCCEEEE
AEPDLVAECIAKMQQVVDIPVHVKHRIGIDDMQSYDEMLNFVDTVAKTGCHHFVVHARIA
CCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LLKGLSPKENREIPPLRYEDVYRLKQDRPELFIEINGGIKTVAETQAHLQHVDGVMIGRE
HHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEECHH
AYHNPYLLAELGQLWNLDLPDRFNIMQQMMPYIEQRLAEGAPLSIITRHILGLFQNLPGA
HHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCH
RKWRQALSGGNAKTRSDIEHALCNIHEAIQRTEASLQVQDI
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEECC
>Mature Secondary Structure
MFGSVFMKSSLETLKPRISVAPMMDWTTKDYRFFARLFNPNVVLYTEMVTTGAILFGDTK
CCCHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHCCCEEEEEEHHHHCEEEECCCH
RHLDYSHGEHPIVLQLGGSNPKDLAECTKMAQDWGYDEVNLNVGCPSDRVQNNKIGACLM
HHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCCCCCEEEE
AEPDLVAECIAKMQQVVDIPVHVKHRIGIDDMQSYDEMLNFVDTVAKTGCHHFVVHARIA
CCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LLKGLSPKENREIPPLRYEDVYRLKQDRPELFIEINGGIKTVAETQAHLQHVDGVMIGRE
HHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEECHH
AYHNPYLLAELGQLWNLDLPDRFNIMQQMMPYIEQRLAEGAPLSIITRHILGLFQNLPGA
HHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCH
RKWRQALSGGNAKTRSDIEHALCNIHEAIQRTEASLQVQDI
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]