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The map label for this gene is pntB [H]

Identifier: 50086089

GI number: 50086089

Start: 3004854

End: 3006308

Strand: Reverse

Name: pntB [H]

Synonym: ACIAD3078

Alternate gene names: 50086089

Gene position: 3006308-3004854 (Counterclockwise)

Preceding gene: 50086090

Following gene: 50086088

Centisome position: 83.54

GC content: 47.35

Gene sequence:

>1455_bases
ATGGAATTTATTCGAGCGAATGCAGAGTGGTTCTACCTCATTGGTGCTGTCCTGTTTATTATGACGCTACGTGGTTTGTC
TGGCCCTAAAACAGCAATTTCAGGCAACCGCTACGGCATGATCGGTATGGCGATTGCGGTATTGACCACGTTCTTTGTCG
CGGATCATCCTGTGGTCTGGATGATTGTTGGGGCAATGGTGTTGGGTGCGATTGTGGGGATTGTGCGTGCACGTACAGTA
CCGATGACACAAATGCCAGAAACGGTTGCACTGATGCACTCTCTGGTGGGTTTGGCAGCAGTACTCATTGCGATTGCGGC
AATTTTGCATAACAACCAATTAACAGTTCTGTTCGAACAAAACGAAGCAGCATTGACTGCTGCGGGCGTTCAGCATGCAC
ATATGAGCCCAGTGCATTTATTTGAACTGTTTGTAGGGTGTTTTGTCGGTGCGATTACCTTTACAGCTTCGGTGTTTGCC
TACGGTAAACTGGCTGCAAAGAAATGGGCAAAAACCATTTCTGGCGCTTGGGTTAAACCTGTTCAGGCAATCATCTTTAT
TGCGATGTTGGCTTGCGGTTTTTACTTCTTCTCGACGGGCAATATGACGGCATTCTGGGCAATGACCATATTGGCACTTG
CATTTGGCTGGGTCTGGATTGCGCCTGTCGGTGGCGGTGATATGCCAGTAGTGGTGTCACTTCTCAACTCATTCTCTGGA
TGGGCGGCAGCAGGGATTGGTTTTACCTTAGAAAATAACATGCTGATTGTGGCGGGTTCATTAGTCGGTTCATCGGGTGC
GATCCTGTCTTACATTATGTGTAAAGCCATGAACCGTAACATCTTAAATGTTCTGTTTGGTGGCGCAATGGGCGGTGCTG
CGGTTGCGGGTGCTGCCAAAGGTGAGCAGGTTCAACGTAACCATCGTTCAGGTTCGGCAGATGATGCAGGCTTCTTGATG
TCGAATGCAGACAGTGTGGTGATTGTACCAGGTTATGGTATGGCACAAGGTCGTGCACAAAATGCGGTAAAAGAACTTTG
CGAATTGCTCAAAGAGCAGGGCGTAAAGGTGCGTTTTGCAATTCACCCAGTGGCGGGGCGTATGCCAGGTCACATGAACG
TACTTTTGGCAGAAGCTGACGTTGCATATGAAGACATTTTGGAAATGGATGAGATTAACTCAGACTTTCCAGCAACCGAC
GTCGTACTTGTCATTGGTGCCAATGACGTCGTCAATCCGGCTGCCAAAGATGATCCAAGCTCTCCAATTTATGGTATGCC
GATTTTGGAAGCACACAAGGCACGAACCATTATGGTGATCAAACGTTCAATGGCAACAGGTTATGCAGGTCTGGATAATG
ATTTGTTCTATAACGAAAAAACCATGATGATTTTTGGTGATGCCAAGAAAGTGGTGGAAGATATGACCAAAGCCATTAAT
GGCAATGGTCACTAA

Upstream 100 bases:

>100_bases
GGTGTTCTTGGCCAGTATTAACATTTTTGGTGGTTTCGCAGTGACTGCGCGCATGCTTGAAATGTTTAAGCCGAAGCAGA
AAAAATAAGAGGACGAAAAC

Downstream 100 bases:

>100_bases
TCTGAGCTAAAACGAAAACCACCTGCGGGTGGTTTTTTTACGGATGAATAAAGCTTTATTTTATACGCATCGATGTATGA
TAGTTTTATATGCGATGATG

Product: pyridine nucleotide transhydrogenase, beta subunit

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MEFIRANAEWFYLIGAVLFIMTLRGLSGPKTAISGNRYGMIGMAIAVLTTFFVADHPVVWMIVGAMVLGAIVGIVRARTV
PMTQMPETVALMHSLVGLAAVLIAIAAILHNNQLTVLFEQNEAALTAAGVQHAHMSPVHLFELFVGCFVGAITFTASVFA
YGKLAAKKWAKTISGAWVKPVQAIIFIAMLACGFYFFSTGNMTAFWAMTILALAFGWVWIAPVGGGDMPVVVSLLNSFSG
WAAAGIGFTLENNMLIVAGSLVGSSGAILSYIMCKAMNRNILNVLFGGAMGGAAVAGAAKGEQVQRNHRSGSADDAGFLM
SNADSVVIVPGYGMAQGRAQNAVKELCELLKEQGVKVRFAIHPVAGRMPGHMNVLLAEADVAYEDILEMDEINSDFPATD
VVLVIGANDVVNPAAKDDPSSPIYGMPILEAHKARTIMVIKRSMATGYAGLDNDLFYNEKTMMIFGDAKKVVEDMTKAIN
GNGH

Sequences:

>Translated_484_residues
MEFIRANAEWFYLIGAVLFIMTLRGLSGPKTAISGNRYGMIGMAIAVLTTFFVADHPVVWMIVGAMVLGAIVGIVRARTV
PMTQMPETVALMHSLVGLAAVLIAIAAILHNNQLTVLFEQNEAALTAAGVQHAHMSPVHLFELFVGCFVGAITFTASVFA
YGKLAAKKWAKTISGAWVKPVQAIIFIAMLACGFYFFSTGNMTAFWAMTILALAFGWVWIAPVGGGDMPVVVSLLNSFSG
WAAAGIGFTLENNMLIVAGSLVGSSGAILSYIMCKAMNRNILNVLFGGAMGGAAVAGAAKGEQVQRNHRSGSADDAGFLM
SNADSVVIVPGYGMAQGRAQNAVKELCELLKEQGVKVRFAIHPVAGRMPGHMNVLLAEADVAYEDILEMDEINSDFPATD
VVLVIGANDVVNPAAKDDPSSPIYGMPILEAHKARTIMVIKRSMATGYAGLDNDLFYNEKTMMIFGDAKKVVEDMTKAIN
GNGH
>Mature_484_residues
MEFIRANAEWFYLIGAVLFIMTLRGLSGPKTAISGNRYGMIGMAIAVLTTFFVADHPVVWMIVGAMVLGAIVGIVRARTV
PMTQMPETVALMHSLVGLAAVLIAIAAILHNNQLTVLFEQNEAALTAAGVQHAHMSPVHLFELFVGCFVGAITFTASVFA
YGKLAAKKWAKTISGAWVKPVQAIIFIAMLACGFYFFSTGNMTAFWAMTILALAFGWVWIAPVGGGDMPVVVSLLNSFSG
WAAAGIGFTLENNMLIVAGSLVGSSGAILSYIMCKAMNRNILNVLFGGAMGGAAVAGAAKGEQVQRNHRSGSADDAGFLM
SNADSVVIVPGYGMAQGRAQNAVKELCELLKEQGVKVRFAIHPVAGRMPGHMNVLLAEADVAYEDILEMDEINSDFPATD
VVLVIGANDVVNPAAKDDPSSPIYGMPILEAHKARTIMVIKRSMATGYAGLDNDLFYNEKTMMIFGDAKKVVEDMTKAIN
GNGH

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=483, Percent_Identity=42.0289855072464, Blast_Score=348, Evalue=9e-96,
Organism=Homo sapiens, GI122939153, Length=483, Percent_Identity=42.0289855072464, Blast_Score=348, Evalue=9e-96,
Organism=Escherichia coli, GI1787886, Length=481, Percent_Identity=50.3118503118503, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17550456, Length=481, Percent_Identity=39.5010395010395, Blast_Score=326, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 51437; Mature: 51437

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFIRANAEWFYLIGAVLFIMTLRGLSGPKTAISGNRYGMIGMAIAVLTTFFVADHPVVW
CCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
MIVGAMVLGAIVGIVRARTVPMTQMPETVALMHSLVGLAAVLIAIAAILHNNQLTVLFEQ
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
NEAALTAAGVQHAHMSPVHLFELFVGCFVGAITFTASVFAYGKLAAKKWAKTISGAWVKP
CCCEEEHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VQAIIFIAMLACGFYFFSTGNMTAFWAMTILALAFGWVWIAPVGGGDMPVVVSLLNSFSG
HHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCC
WAAAGIGFTLENNMLIVAGSLVGSSGAILSYIMCKAMNRNILNVLFGGAMGGAAVAGAAK
HHHHCCCEEEECCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHCCCC
GEQVQRNHRSGSADDAGFLMSNADSVVIVPGYGMAQGRAQNAVKELCELLKEQGVKVRFA
CHHHHHHCCCCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
IHPVAGRMPGHMNVLLAEADVAYEDILEMDEINSDFPATDVVLVIGANDVVNPAAKDDPS
EECCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCC
SPIYGMPILEAHKARTIMVIKRSMATGYAGLDNDLFYNEKTMMIFGDAKKVVEDMTKAIN
CCCCCCCCHHHCCCCEEEEEEHHHHCCCCCCCCCEEECCCEEEEECCHHHHHHHHHHHHC
GNGH
CCCC
>Mature Secondary Structure
MEFIRANAEWFYLIGAVLFIMTLRGLSGPKTAISGNRYGMIGMAIAVLTTFFVADHPVVW
CCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
MIVGAMVLGAIVGIVRARTVPMTQMPETVALMHSLVGLAAVLIAIAAILHNNQLTVLFEQ
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
NEAALTAAGVQHAHMSPVHLFELFVGCFVGAITFTASVFAYGKLAAKKWAKTISGAWVKP
CCCEEEHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VQAIIFIAMLACGFYFFSTGNMTAFWAMTILALAFGWVWIAPVGGGDMPVVVSLLNSFSG
HHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCC
WAAAGIGFTLENNMLIVAGSLVGSSGAILSYIMCKAMNRNILNVLFGGAMGGAAVAGAAK
HHHHCCCEEEECCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHCCCC
GEQVQRNHRSGSADDAGFLMSNADSVVIVPGYGMAQGRAQNAVKELCELLKEQGVKVRFA
CHHHHHHCCCCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
IHPVAGRMPGHMNVLLAEADVAYEDILEMDEINSDFPATDVVLVIGANDVVNPAAKDDPS
EECCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCC
SPIYGMPILEAHKARTIMVIKRSMATGYAGLDNDLFYNEKTMMIFGDAKKVVEDMTKAIN
CCCCCCCCHHHCCCCEEEEEEHHHHCCCCCCCCCEEECCCEEEEECCHHHHHHHHHHHHC
GNGH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]