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The map label for this gene is rumA

Identifier: 50086080

GI number: 50086080

Start: 2995133

End: 2996524

Strand: Reverse

Name: rumA

Synonym: ACIAD3069

Alternate gene names: 50086080

Gene position: 2996524-2995133 (Counterclockwise)

Preceding gene: 50086081

Following gene: 50086079

Centisome position: 83.27

GC content: 41.31

Gene sequence:

>1392_bases
TTGAAAAAACATGCTTCATCTCGTTCAAAATCTGCAACGGTCTATACGTTTCGGATAGAATCTTTTTCTCATGAAGGACG
AGGGATAGCACATTTTGGTGAGTTCGCTGAACATCCTCAGACAAAACATGGCAAAAAGGTATTTGTTCGCTATGCTTTAC
CTGGAGAGGTTGTTCGGGCAAAAATCACCCACGAAACCAAACGTTTTGAAGAAGGTGAGCTGATTGAAATTCTCGATCAG
CCTTCTATTGATCGTATTGTGCCTGTCTGTCCGCATTTTGGTGTTTGTGGTGGTTGTAGTATGCAACATATTACGCCTAA
CCAACAAATTCAGATCAAGCAGCATGTATTGGCGTCGCATTTACAGCATTTTGCTGGTTTAACCGTTAATGAGTGGCTAA
CGCCAGTCCGTTCACTACGTGCAGATTATCGACGTCGCGCACGCGTAGGCGTGCGTTATATTCCGGCAAAACAAAAGCTG
ATCATGGGCTTTCGTGAGCATGGGAGTAATCGTTTAACACCCATTCAAAGCTGTGCTGTTTTAGATCAAAGACTTTCTCA
AGCATTACCCGAACTTTATGATGTGTTAAATCGGCTTGTTGCCAAAGCTGAGATTGGTCATATTGAGCTGGCAATGGGCG
ATGATGAGGTTTCATTACTGGTTCGACATCTCAACACTTTACCAGATAGTGATATCACTCAATTGTGCGATTATGCGCAG
CATAAAAACTGGCAACTCTATTTACAGGCAAAGGGTTCAGATAGCTTAAAGCGCGTAGACCGATCTGGTGCAGAGATGCG
ATTGCATTATGCTGTACCTGCTTTTAACCTAAATTTTGCGTTTTCTCCACTTGATTTTACACAAGTCAACGCAACCGTAA
ATCAACAAATGTTAAAATTGGCATGTGATTTGCTTAAGTTGCAAAAAGGTGAGCATGTTCTTGACTTATTTTGCGGGTTG
GGAAATTTTTCGCTGCCTTTGGCGCGTTGTGTCGGTGACACAGGACGTGTGGTGGGTGTAGAGGGAAGTGAAGAGATGGT
TCAACGAGCGTTTGAAAATGCAGCCCAAAATGCTTTGCACAATGTTGAGTTCTATTCACAAGATTTAACAAAAGATTTTT
CGCATCATTCTTGGGCAAATCAAGGATTTGATGCATTATTGATTGATCCTCCACGTTCCGGTGCATTTGAAATCATGCAC
TATATTGCGAATTTTGAGGCAAAAAGAATCGTTTATGTATCATGTAATCCAGCAACATTGGCAAGGGATGCAGGTGTACT
GGCTCAACATGGTTATCAGTTAAAGAAAGTAGGCGTTATGGACATGTTTACACATACTGAACATGTTGAATCAATTGCTT
TATTTGAGAAAATTCAAGAGATAAACGATTAA

Upstream 100 bases:

>100_bases
CACAACAGAACGAATTTTTAAGTGTCTGGCAGAAAACCGCTCAGCACACGACATTTACATCGCAATATTTTCCTTGCACT
TCGCAGGAAGAAGGTTCCTC

Downstream 100 bases:

>100_bases
AAACATGAGTTACAACACCATAGGAGAATGGTATGGTCACAGTACGTGAGCAGTTGCCTGAGCGACTCTCAGAGTTGGCT
GAGGAAACGACTGTAGAGCA

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKKHASSRSKSATVYTFRIESFSHEGRGIAHFGEFAEHPQTKHGKKVFVRYALPGEVVRAKITHETKRFEEGELIEILDQ
PSIDRIVPVCPHFGVCGGCSMQHITPNQQIQIKQHVLASHLQHFAGLTVNEWLTPVRSLRADYRRRARVGVRYIPAKQKL
IMGFREHGSNRLTPIQSCAVLDQRLSQALPELYDVLNRLVAKAEIGHIELAMGDDEVSLLVRHLNTLPDSDITQLCDYAQ
HKNWQLYLQAKGSDSLKRVDRSGAEMRLHYAVPAFNLNFAFSPLDFTQVNATVNQQMLKLACDLLKLQKGEHVLDLFCGL
GNFSLPLARCVGDTGRVVGVEGSEEMVQRAFENAAQNALHNVEFYSQDLTKDFSHHSWANQGFDALLIDPPRSGAFEIMH
YIANFEAKRIVYVSCNPATLARDAGVLAQHGYQLKKVGVMDMFTHTEHVESIALFEKIQEIND

Sequences:

>Translated_463_residues
MKKHASSRSKSATVYTFRIESFSHEGRGIAHFGEFAEHPQTKHGKKVFVRYALPGEVVRAKITHETKRFEEGELIEILDQ
PSIDRIVPVCPHFGVCGGCSMQHITPNQQIQIKQHVLASHLQHFAGLTVNEWLTPVRSLRADYRRRARVGVRYIPAKQKL
IMGFREHGSNRLTPIQSCAVLDQRLSQALPELYDVLNRLVAKAEIGHIELAMGDDEVSLLVRHLNTLPDSDITQLCDYAQ
HKNWQLYLQAKGSDSLKRVDRSGAEMRLHYAVPAFNLNFAFSPLDFTQVNATVNQQMLKLACDLLKLQKGEHVLDLFCGL
GNFSLPLARCVGDTGRVVGVEGSEEMVQRAFENAAQNALHNVEFYSQDLTKDFSHHSWANQGFDALLIDPPRSGAFEIMH
YIANFEAKRIVYVSCNPATLARDAGVLAQHGYQLKKVGVMDMFTHTEHVESIALFEKIQEIND
>Mature_463_residues
MKKHASSRSKSATVYTFRIESFSHEGRGIAHFGEFAEHPQTKHGKKVFVRYALPGEVVRAKITHETKRFEEGELIEILDQ
PSIDRIVPVCPHFGVCGGCSMQHITPNQQIQIKQHVLASHLQHFAGLTVNEWLTPVRSLRADYRRRARVGVRYIPAKQKL
IMGFREHGSNRLTPIQSCAVLDQRLSQALPELYDVLNRLVAKAEIGHIELAMGDDEVSLLVRHLNTLPDSDITQLCDYAQ
HKNWQLYLQAKGSDSLKRVDRSGAEMRLHYAVPAFNLNFAFSPLDFTQVNATVNQQMLKLACDLLKLQKGEHVLDLFCGL
GNFSLPLARCVGDTGRVVGVEGSEEMVQRAFENAAQNALHNVEFYSQDLTKDFSHHSWANQGFDALLIDPPRSGAFEIMH
YIANFEAKRIVYVSCNPATLARDAGVLAQHGYQLKKVGVMDMFTHTEHVESIALFEKIQEIND

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=201, Percent_Identity=34.3283582089552, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI51173878, Length=201, Percent_Identity=34.3283582089552, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1789148, Length=455, Percent_Identity=37.3626373626374, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1790403, Length=324, Percent_Identity=27.7777777777778, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787083, Length=389, Percent_Identity=24.6786632390745, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24666579, Length=204, Percent_Identity=31.3725490196078, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_ACIAD (Q6F847)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047590.1
- ProteinModelPortal:   Q6F847
- SMR:   Q6F847
- STRING:   Q6F847
- GeneID:   2880554
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3069
- NMPDR:   fig|62977.3.peg.2783
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   VECRILE
- PhylomeDB:   Q6F847
- ProtClustDB:   PRK13168
- BioCyc:   ASP62977:ACIAD3069-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 52204; Mature: 52204

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 415-415 BINDING 288-288 BINDING 317-317 BINDING 322-322 BINDING 341-341 BINDING 368-368 BINDING 389-389

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHASSRSKSATVYTFRIESFSHEGRGIAHFGEFAEHPQTKHGKKVFVRYALPGEVVRA
CCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHH
KITHETKRFEEGELIEILDQPSIDRIVPVCPHFGVCGGCSMQHITPNQQIQIKQHVLASH
HHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LQHFAGLTVNEWLTPVRSLRADYRRRARVGVRYIPAKQKLIMGFREHGSNRLTPIQSCAV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCCCHHHHHHH
LDQRLSQALPELYDVLNRLVAKAEIGHIELAMGDDEVSLLVRHLNTLPDSDITQLCDYAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHH
HKNWQLYLQAKGSDSLKRVDRSGAEMRLHYAVPAFNLNFAFSPLDFTQVNATVNQQMLKL
CCCEEEEEEECCCHHHHHHHCCCCEEEEEEECCEEECCEEECCCCHHHHHHHHHHHHHHH
ACDLLKLQKGEHVLDLFCGLGNFSLPLARCVGDTGRVVGVEGSEEMVQRAFENAAQNALH
HHHHHHHCCCCHHHHHHHCCCCCCCHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHH
NVEFYSQDLTKDFSHHSWANQGFDALLIDPPRSGAFEIMHYIANFEAKRIVYVSCNPATL
HHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCHHH
ARDAGVLAQHGYQLKKVGVMDMFTHTEHVESIALFEKIQEIND
HHHCCHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKHASSRSKSATVYTFRIESFSHEGRGIAHFGEFAEHPQTKHGKKVFVRYALPGEVVRA
CCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHH
KITHETKRFEEGELIEILDQPSIDRIVPVCPHFGVCGGCSMQHITPNQQIQIKQHVLASH
HHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LQHFAGLTVNEWLTPVRSLRADYRRRARVGVRYIPAKQKLIMGFREHGSNRLTPIQSCAV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCCCHHHHHHH
LDQRLSQALPELYDVLNRLVAKAEIGHIELAMGDDEVSLLVRHLNTLPDSDITQLCDYAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHH
HKNWQLYLQAKGSDSLKRVDRSGAEMRLHYAVPAFNLNFAFSPLDFTQVNATVNQQMLKL
CCCEEEEEEECCCHHHHHHHCCCCEEEEEEECCEEECCEEECCCCHHHHHHHHHHHHHHH
ACDLLKLQKGEHVLDLFCGLGNFSLPLARCVGDTGRVVGVEGSEEMVQRAFENAAQNALH
HHHHHHHCCCCHHHHHHHCCCCCCCHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHH
NVEFYSQDLTKDFSHHSWANQGFDALLIDPPRSGAFEIMHYIANFEAKRIVYVSCNPATL
HHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCHHH
ARDAGVLAQHGYQLKKVGVMDMFTHTEHVESIALFEKIQEIND
HHHCCHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA