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The map label for this gene is mazG [H]

Identifier: 50086077

GI number: 50086077

Start: 2991084

End: 2991851

Strand: Reverse

Name: mazG [H]

Synonym: ACIAD3065

Alternate gene names: 50086077

Gene position: 2991851-2991084 (Counterclockwise)

Preceding gene: 50086079

Following gene: 50086076

Centisome position: 83.14

GC content: 40.76

Gene sequence:

>768_bases
GTGGAAAAACTGTTAGATATCATGCAAACCTTGCGTTTAGAGTGTCCTTGGGATCGTGAACAAACGCCTTTAAGCTTAAC
CCGATATGCGATTGAAGAAGCCTATGAAGTAGAAGCTGCTGTTCGCTCAGGTGATCTGAATGAAATCCGTCAGGAGCTTG
GTGATTTACTGTTACAGGTGGTTTTTCAGTCGCAGATGTATAAGGAACAAGGTGCTTTTGATTTTCAGGATGTTGTGGCG
ACCTTAAGTGAAAAGTTGATCCGTCGTCATCCACACGTATTTCAGGCAGAGCAATTCTCTGCTTTAGATTCAGAACAGGT
GAGTGAACTTTGGCAGCAGATTAAACAACAGGAAAAACAAGGTAAAAGTCGTTCAAGACTAGATGACATTAAGCCTGGAC
CTGCTTTAAATCAGGCACAGCATATTCAAAAAAAAGCAGCGCAATTGGGCTTTGATTTTGACAATCTTGAAGATGCCTAT
GGAAAGCTCAAAGAAGAGCTAAGTGAGTTTGAAGCTGCTCTGCATAAGCAGGATGTAGATCATATTTTTGAAGAATTTGG
TGATTGTTTATTTTCATTGGTCAATGTTGGACGTAAACTAGGCCTATCGAGTGAATCGGCACTACTTGCTACAATTCACA
AGTTTAGACAGCGTTTTGCTTATATTGAGAAGCAGGCGCAATTACAACATAAAGTGCTTGAAGAGATGAGTCTGCAAGAG
ATAGACGGGCTCTGGAATCAAGCCAAACAACATCTGAATAATGGTTAA

Upstream 100 bases:

>100_bases
GTTTAATGGATGAAGACGATGTATTGGAGAAGATGGATGCATGATAGCAAACCTCATGATAAACAGATTAAATTTTGAGT
GACTTAAGCGGAGACATTTT

Downstream 100 bases:

>100_bases
TTCATGTCAATACGTCATTTTTTTACATTTTTTTGGGCGACATTGCTTACACTTTTTTCTATGCAAATTGTTTTTGCACA
AGGGTTTGATCAACAATATG

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MEKLLDIMQTLRLECPWDREQTPLSLTRYAIEEAYEVEAAVRSGDLNEIRQELGDLLLQVVFQSQMYKEQGAFDFQDVVA
TLSEKLIRRHPHVFQAEQFSALDSEQVSELWQQIKQQEKQGKSRSRLDDIKPGPALNQAQHIQKKAAQLGFDFDNLEDAY
GKLKEELSEFEAALHKQDVDHIFEEFGDCLFSLVNVGRKLGLSSESALLATIHKFRQRFAYIEKQAQLQHKVLEEMSLQE
IDGLWNQAKQHLNNG

Sequences:

>Translated_255_residues
MEKLLDIMQTLRLECPWDREQTPLSLTRYAIEEAYEVEAAVRSGDLNEIRQELGDLLLQVVFQSQMYKEQGAFDFQDVVA
TLSEKLIRRHPHVFQAEQFSALDSEQVSELWQQIKQQEKQGKSRSRLDDIKPGPALNQAQHIQKKAAQLGFDFDNLEDAY
GKLKEELSEFEAALHKQDVDHIFEEFGDCLFSLVNVGRKLGLSSESALLATIHKFRQRFAYIEKQAQLQHKVLEEMSLQE
IDGLWNQAKQHLNNG
>Mature_255_residues
MEKLLDIMQTLRLECPWDREQTPLSLTRYAIEEAYEVEAAVRSGDLNEIRQELGDLLLQVVFQSQMYKEQGAFDFQDVVA
TLSEKLIRRHPHVFQAEQFSALDSEQVSELWQQIKQQEKQGKSRSRLDDIKPGPALNQAQHIQKKAAQLGFDFDNLEDAY
GKLKEELSEFEAALHKQDVDHIFEEFGDCLFSLVNVGRKLGLSSESALLATIHKFRQRFAYIEKQAQLQHKVLEEMSLQE
IDGLWNQAKQHLNNG

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=258, Percent_Identity=42.6356589147287, Blast_Score=186, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 29474; Mature: 29474

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]