Click here to switch to the map view.

The map label for this gene is pcnB [H]

Identifier: 50086075

GI number: 50086075

Start: 2989112

End: 2990569

Strand: Reverse

Name: pcnB [H]

Synonym: ACIAD3063

Alternate gene names: 50086075

Gene position: 2990569-2989112 (Counterclockwise)

Preceding gene: 50086076

Following gene: 50086074

Centisome position: 83.1

GC content: 45.4

Gene sequence:

>1458_bases
TTGCAAACCTTGCGCGCGTCAAAATGTGGGTTATCGACTACACAATTACCTGCTTCTATTTTAGATGTGATTGATAGTCT
AAACAAAGCAGGCTATGAAGCATATATCGTTGGTGGTGGTGTTCGGGATTTGATGCTTGGACTAAATCCAAAAGATTTCG
ATGCTGTTACCAATGCCACACCAGCTCAGGTGAAAGAAGTTTTTGGGCGTCGTTGCCGAATTATTGGGCGCCGATTTGAA
TTGGCCCATGTTTACTCAGGCCGAGAACTGATCGAAGTTGCGACTTTTCGTGCACCACCAAAAAAAGCAGTCACCAGTGC
ATCTGGCATGATTTTGCGTGATAATAACTGGGGAACGATTGAGCAAGACTTTGCTCGTCGGGATTTCTCAATTAATACCT
TATATTATCAGCCACGCAAAGGAATCGTACTCGACTTTTGTGATGCTGTTTCAGATGTCAAAAAAAGAACCCTACGTCTG
TTAGGCGACCCAGCCCAGCGTTTTGAAGAAGATCCTGTGCGTATGCTGCGAGCATTACGCTTTGCAGCCAAATTGAATTT
TGACATCGATCCCGAGATTCTTAAGATCTTTGATGTTGAAATGACCCAGTTGCTTAGAGATGTGTCACCACATCGTCTTT
ATGATGAATCGCAAAAGCTATTTACTATGGGGCATTTGTCCCGTGTATTGCCGATGCTGATCGAATTTGATATCTGGCGT
CAACTGTTTGCTGATGTCAAAGCTGAAATTACTCCTTTCATTGAACGTGCAGCGAAAAACACAGATCAGCGTATCCAGAT
TGGCAAGACCATTAATCCTGCATTTTTCTATGCGGTTTTGCTCTGGCGTTCGTTCTTGGAGCGTAGTGACTTTTATTTGC
AAAAAGGTATTGTCCCTGCTGAAGCGCGTGCACAGGCTGGGCTGGATGTACTCAAGCGTCAGGCAACACGCACCGTGATT
CCACGTTTTGCAGAAACTTTTATTCGTGAAGTATGGGAAATGCAAACCCGTCTTTTAAATCCGAAGCCACAGCAGATTGA
GGCATTGGCGGGGCATGCCAGATTCCGTGCTGGATTTGACTTTTTGTTGTTGCGTGAGAAGTCTGGAGATTCTTCTACTC
AGGGGATGGGCGAGTGGTGGGAGCATTATCAGTCCTTGAATGCCGATCAAAAAGAGGTGGTGATTAGCCAATATAATCGT
CAACGTGCAAAAACTCGCCGTAAAGTGCAGCAAGATGCCGTTGAGCAAAAAGAATCGGCAGAGATTGAACCGCTAGTCGA
TGTACCAGAACCGCGTAGTCGCCGTGACCGCAAAACACAAAATAGATCATCATCTGCACCTCAGCGTAACGACTCATCTC
AAGGCTCGGAATCTCATTTTGGCGCAGATCATCCTATTCTAAAACGTAAACGCGTGAAAAGAGATCTGGCACAGGTGGTC
TTTGGACCAACCCAATGA

Upstream 100 bases:

>100_bases
CGAATAAAGCAGTGAGAGAATCAAATTGCCCTTTACTCAGGTTAGAGATATGATTAGCAACATCTTATTATTAACGTCCT
GACGTAGGAGACAGCGTCTT

Downstream 100 bases:

>100_bases
GCATAACCACCTATATCGGAATGGGAAGTAATCTGGGAGATTCGCGCCAGTTGCTTCAACAAGCAGTTCGACAGTTGCAA
ACATTGGGACAGGTACAAGT

Product: poly(A) polymerase I (PAP)

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MQTLRASKCGLSTTQLPASILDVIDSLNKAGYEAYIVGGGVRDLMLGLNPKDFDAVTNATPAQVKEVFGRRCRIIGRRFE
LAHVYSGRELIEVATFRAPPKKAVTSASGMILRDNNWGTIEQDFARRDFSINTLYYQPRKGIVLDFCDAVSDVKKRTLRL
LGDPAQRFEEDPVRMLRALRFAAKLNFDIDPEILKIFDVEMTQLLRDVSPHRLYDESQKLFTMGHLSRVLPMLIEFDIWR
QLFADVKAEITPFIERAAKNTDQRIQIGKTINPAFFYAVLLWRSFLERSDFYLQKGIVPAEARAQAGLDVLKRQATRTVI
PRFAETFIREVWEMQTRLLNPKPQQIEALAGHARFRAGFDFLLLREKSGDSSTQGMGEWWEHYQSLNADQKEVVISQYNR
QRAKTRRKVQQDAVEQKESAEIEPLVDVPEPRSRRDRKTQNRSSSAPQRNDSSQGSESHFGADHPILKRKRVKRDLAQVV
FGPTQ

Sequences:

>Translated_485_residues
MQTLRASKCGLSTTQLPASILDVIDSLNKAGYEAYIVGGGVRDLMLGLNPKDFDAVTNATPAQVKEVFGRRCRIIGRRFE
LAHVYSGRELIEVATFRAPPKKAVTSASGMILRDNNWGTIEQDFARRDFSINTLYYQPRKGIVLDFCDAVSDVKKRTLRL
LGDPAQRFEEDPVRMLRALRFAAKLNFDIDPEILKIFDVEMTQLLRDVSPHRLYDESQKLFTMGHLSRVLPMLIEFDIWR
QLFADVKAEITPFIERAAKNTDQRIQIGKTINPAFFYAVLLWRSFLERSDFYLQKGIVPAEARAQAGLDVLKRQATRTVI
PRFAETFIREVWEMQTRLLNPKPQQIEALAGHARFRAGFDFLLLREKSGDSSTQGMGEWWEHYQSLNADQKEVVISQYNR
QRAKTRRKVQQDAVEQKESAEIEPLVDVPEPRSRRDRKTQNRSSSAPQRNDSSQGSESHFGADHPILKRKRVKRDLAQVV
FGPTQ
>Mature_485_residues
MQTLRASKCGLSTTQLPASILDVIDSLNKAGYEAYIVGGGVRDLMLGLNPKDFDAVTNATPAQVKEVFGRRCRIIGRRFE
LAHVYSGRELIEVATFRAPPKKAVTSASGMILRDNNWGTIEQDFARRDFSINTLYYQPRKGIVLDFCDAVSDVKKRTLRL
LGDPAQRFEEDPVRMLRALRFAAKLNFDIDPEILKIFDVEMTQLLRDVSPHRLYDESQKLFTMGHLSRVLPMLIEFDIWR
QLFADVKAEITPFIERAAKNTDQRIQIGKTINPAFFYAVLLWRSFLERSDFYLQKGIVPAEARAQAGLDVLKRQATRTVI
PRFAETFIREVWEMQTRLLNPKPQQIEALAGHARFRAGFDFLLLREKSGDSSTQGMGEWWEHYQSLNADQKEVVISQYNR
QRAKTRRKVQQDAVEQKESAEIEPLVDVPEPRSRRDRKTQNRSSSAPQRNDSSQGSESHFGADHPILKRKRVKRDLAQVV
FGPTQ

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=241, Percent_Identity=29.8755186721992, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI87081691, Length=403, Percent_Identity=41.1910669975186, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1789436, Length=393, Percent_Identity=28.2442748091603, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=228, Percent_Identity=29.3859649122807, Blast_Score=89, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6321016, Length=79, Percent_Identity=44.3037974683544, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281360112, Length=207, Percent_Identity=30.9178743961353, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21357337, Length=207, Percent_Identity=30.9178743961353, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 55601; Mature: 55601

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTLRASKCGLSTTQLPASILDVIDSLNKAGYEAYIVGGGVRDLMLGLNPKDFDAVTNAT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCHHHHHCCCC
PAQVKEVFGRRCRIIGRRFELAHVYSGRELIEVATFRAPPKKAVTSASGMILRDNNWGTI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHCCCCEEEECCCCCCH
EQDFARRDFSINTLYYQPRKGIVLDFCDAVSDVKKRTLRLLGDPAQRFEEDPVRMLRALR
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHH
FAAKLNFDIDPEILKIFDVEMTQLLRDVSPHRLYDESQKLFTMGHLSRVLPMLIEFDIWR
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLFADVKAEITPFIERAAKNTDQRIQIGKTINPAFFYAVLLWRSFLERSDFYLQKGIVPA
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EARAQAGLDVLKRQATRTVIPRFAETFIREVWEMQTRLLNPKPQQIEALAGHARFRAGFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
FLLLREKSGDSSTQGMGEWWEHYQSLNADQKEVVISQYNRQRAKTRRKVQQDAVEQKESA
EEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EIEPLVDVPEPRSRRDRKTQNRSSSAPQRNDSSQGSESHFGADHPILKRKRVKRDLAQVV
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FGPTQ
CCCCC
>Mature Secondary Structure
MQTLRASKCGLSTTQLPASILDVIDSLNKAGYEAYIVGGGVRDLMLGLNPKDFDAVTNAT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCHHHHHCCCC
PAQVKEVFGRRCRIIGRRFELAHVYSGRELIEVATFRAPPKKAVTSASGMILRDNNWGTI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHCCCCEEEECCCCCCH
EQDFARRDFSINTLYYQPRKGIVLDFCDAVSDVKKRTLRLLGDPAQRFEEDPVRMLRALR
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHH
FAAKLNFDIDPEILKIFDVEMTQLLRDVSPHRLYDESQKLFTMGHLSRVLPMLIEFDIWR
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLFADVKAEITPFIERAAKNTDQRIQIGKTINPAFFYAVLLWRSFLERSDFYLQKGIVPA
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EARAQAGLDVLKRQATRTVIPRFAETFIREVWEMQTRLLNPKPQQIEALAGHARFRAGFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
FLLLREKSGDSSTQGMGEWWEHYQSLNADQKEVVISQYNRQRAKTRRKVQQDAVEQKESA
EEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EIEPLVDVPEPRSRRDRKTQNRSSSAPQRNDSSQGSESHFGADHPILKRKRVKRDLAQVV
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FGPTQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]