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The map label for this gene is sspA [H]

Identifier: 50086031

GI number: 50086031

Start: 2940050

End: 2940700

Strand: Direct

Name: sspA [H]

Synonym: ACIAD3014

Alternate gene names: 50086031

Gene position: 2940050-2940700 (Clockwise)

Preceding gene: 50086030

Following gene: 50086032

Centisome position: 81.7

GC content: 38.86

Gene sequence:

>651_bases
ATGTCTGTCGAAAGTGCATCTGTTCAGGGAATCACGCTGTATAGTCATGCAGATGATTTTCGATCCCACTGGATTCGTTT
TTTACTGGCTGAAAAACAGATCAAGTATCAACTCATTTTAGTTGATCATGAAGATGAAGACTTGGCAAGTTTGAATCCAT
ATAATCAGCTTCCCATGCTGATTGAAAATAATCTCAAACTCTTCTCCACGTCGATTATTTCGGAATATATTGATGATCGC
TATCGACAGAACAAACTGTATGCGGATGCACCTGGCGCACGTGCAGAGCAACGACAATACATCTGGCGCTTCGAGCAGGA
CTGGTTTAAATTGGCAGATACCATGCTCAAACACCCTGATACGTTTGAACCAGCACAACAAGCCAAAGCAAAAAAAGAGT
TAAGTGATACTCTGATATCCTTGAATCCACTTTTTCAACATTTTCCGTATTTTGTTTCTGAAACATTTAGTATTTTAGAT
TGCATGTTGGCTCCCATGTTTATACGATTAAACAGTATGGGAATTGAATTATCAAAGTCAGCAAGCCGTCCTATTTTGCT
GTATTGCAATCGTGTATTTAGTCGTCCTGCTTTTGTTAAATCCATGACTGCGCAGGAAAAAAACCGTTATAGTAACTTTG
TTCAACGTTAA

Upstream 100 bases:

>100_bases
AAGCCTATATACAAAGCCAGCTCAACTTCTTGATCAAGTCATTATCTTTTTAGACGTAATTTTAGTATCCTACAACATTT
AGTTTTTTAGTTTTAGAAAT

Downstream 100 bases:

>100_bases
TTGAGTTCGATATGTCTGAAATTGAAACCACGCTCACGCCTACCCGTCCTTATATGGCACGTGCAATCTATGAATGGATC
TGTGACAATCAACTCACTCC

Product: stringent starvation protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSVESASVQGITLYSHADDFRSHWIRFLLAEKQIKYQLILVDHEDEDLASLNPYNQLPMLIENNLKLFSTSIISEYIDDR
YRQNKLYADAPGARAEQRQYIWRFEQDWFKLADTMLKHPDTFEPAQQAKAKKELSDTLISLNPLFQHFPYFVSETFSILD
CMLAPMFIRLNSMGIELSKSASRPILLYCNRVFSRPAFVKSMTAQEKNRYSNFVQR

Sequences:

>Translated_216_residues
MSVESASVQGITLYSHADDFRSHWIRFLLAEKQIKYQLILVDHEDEDLASLNPYNQLPMLIENNLKLFSTSIISEYIDDR
YRQNKLYADAPGARAEQRQYIWRFEQDWFKLADTMLKHPDTFEPAQQAKAKKELSDTLISLNPLFQHFPYFVSETFSILD
CMLAPMFIRLNSMGIELSKSASRPILLYCNRVFSRPAFVKSMTAQEKNRYSNFVQR
>Mature_215_residues
SVESASVQGITLYSHADDFRSHWIRFLLAEKQIKYQLILVDHEDEDLASLNPYNQLPMLIENNLKLFSTSIISEYIDDRY
RQNKLYADAPGARAEQRQYIWRFEQDWFKLADTMLKHPDTFEPAQQAKAKKELSDTLISLNPLFQHFPYFVSETFSILDC
MLAPMFIRLNSMGIELSKSASRPILLYCNRVFSRPAFVKSMTAQEKNRYSNFVQR

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1789624, Length=209, Percent_Identity=35.8851674641148, Blast_Score=145, Evalue=1e-36,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 25352; Mature: 25221

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVESASVQGITLYSHADDFRSHWIRFLLAEKQIKYQLILVDHEDEDLASLNPYNQLPML
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHCCCCHHCCHH
IENNLKLFSTSIISEYIDDRYRQNKLYADAPGARAEQRQYIWRFEQDWFKLADTMLKHPD
HHCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TFEPAQQAKAKKELSDTLISLNPLFQHFPYFVSETFSILDCMLAPMFIRLNSMGIELSKS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
ASRPILLYCNRVFSRPAFVKSMTAQEKNRYSNFVQR
CCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVESASVQGITLYSHADDFRSHWIRFLLAEKQIKYQLILVDHEDEDLASLNPYNQLPML
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHCCCCHHCCHH
IENNLKLFSTSIISEYIDDRYRQNKLYADAPGARAEQRQYIWRFEQDWFKLADTMLKHPD
HHCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TFEPAQQAKAKKELSDTLISLNPLFQHFPYFVSETFSILDCMLAPMFIRLNSMGIELSKS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
ASRPILLYCNRVFSRPAFVKSMTAQEKNRYSNFVQR
CCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]