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The map label for this gene is amtB [C]

Identifier: 50085982

GI number: 50085982

Start: 2887667

End: 2888884

Strand: Direct

Name: amtB [C]

Synonym: ACIAD2958

Alternate gene names: 50085982

Gene position: 2887667-2888884 (Clockwise)

Preceding gene: 50085979

Following gene: 50085983

Centisome position: 80.24

GC content: 46.96

Gene sequence:

>1218_bases
ATGGGCTTAAGCACTTTTTTTGAGCAAATTATGCAAAATGTCGATTTATTTTTCTTATTGCTCGGCGCAGTGCTGGTTCT
GGCCATGCATGCCGGGTTTGCGTTTTTAGAGCTGGGCACCGTGCGGCATAAGAATCAGGTCAATGCGCTCAGTAAGATTC
TGACCGATTTTTCTATTTCTGCAATTGCTTACTTTTTTGTAGGCTACTACATCTCCTATGGGCACAGCTTCTTTCAAACT
GGTGCTGTGCTCGCCGCGGATCATGGCTATAACCTGATGCGCTGTTTCTTTTTACTAACCTTCGCCGCTGCCATTCCGGC
CATTATTTCAGGCGGTATAGCCGAGCGCGCCAAAATGCGCCCACAGGCCATTGCCACATTTGCACTGGTGGCGTTGGTGT
ATCCACTGTTTGAAGGTATGGCATGGAACGGTAACTTTGGTTTGCAAAAGTGGCTCGAAAGCCAATTTGGCGCGAGTTTT
CACGACTTTGCTGGCTCGGTTGTCGTTCATGCGATGGGTGGCTGGATTGCATTGGCAGCCGTAATGCTCTTGGGTGCTCG
TCATGGTCGTTATAAAAAAGATGGTCGTGTCAGTGCTCATCCGCCCTCTTCCATTCCATTTTTGGCACTAGGCTCATGGA
TTTTGATTGTGGGTTGGTTTGGCTTTAATGTCATGAGTGCTCAGCGTGTCGATGCCATTTCAGGCTTGGTGGCCATCAAT
TCACTGATGGCGATGGTGGGCGGTACCTTAAGCGCCAACTTTATGGGAAAAAATGACCCTGGCTTTTTACACAACGGCCC
ACTGGCAGGCCTGGTCGCCATCTGTGCAGGTTCAGATATTGTGCATCCTGTGAGTGCATTGGTGATCGGTGTTATAGCTG
GAATCATCTTTGTGAAGCTATTTACCTATACACAAAACAAGCTCAAGATCGATGACGTACTTGGTGTATGGCCATTGCAT
GGCGTCTGTGGTGCCCTTGGAGGCCTTGCAGTAGGTGTATTTGGGCAAAAAGCTTTCGGCGGACTGGGTGGTGTCTCAAT
GATGTCTCAACTGATTGGTACGACTTTTGCAATTGTTATTGCATTGGTAGGGGGATTTGTTGTGTATGGCATTTTAAAAG
CAACTCTCGGCATTCGCCTATCGCAAGAAGAAGAATTTCAAGGTGCAGATTTATCTATTCATAAAATATCTGCAAATTCT
GAAGATGGTATGTTTTAA

Upstream 100 bases:

>100_bases
TTTAAATAGGCAAATTAATCCGATCATAGATTGTGATTAATCACCCTTTAGATTTTCTTAACCATTTTGGTGCATAAATA
TCTTTTTTTATGCAGCCAAA

Downstream 100 bases:

>100_bases
ATATTCAGTTTGTGCTTTTGCGGCTCTCAGGGCCGCTTTTTTTATACGCTATTCTTAAGTGATTGCTCATTTTTCTCATT
TGTAATAAAGCGGTCTTTAC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MGLSTFFEQIMQNVDLFFLLLGAVLVLAMHAGFAFLELGTVRHKNQVNALSKILTDFSISAIAYFFVGYYISYGHSFFQT
GAVLAADHGYNLMRCFFLLTFAAAIPAIISGGIAERAKMRPQAIATFALVALVYPLFEGMAWNGNFGLQKWLESQFGASF
HDFAGSVVVHAMGGWIALAAVMLLGARHGRYKKDGRVSAHPPSSIPFLALGSWILIVGWFGFNVMSAQRVDAISGLVAIN
SLMAMVGGTLSANFMGKNDPGFLHNGPLAGLVAICAGSDIVHPVSALVIGVIAGIIFVKLFTYTQNKLKIDDVLGVWPLH
GVCGALGGLAVGVFGQKAFGGLGGVSMMSQLIGTTFAIVIALVGGFVVYGILKATLGIRLSQEEEFQGADLSIHKISANS
EDGMF

Sequences:

>Translated_405_residues
MGLSTFFEQIMQNVDLFFLLLGAVLVLAMHAGFAFLELGTVRHKNQVNALSKILTDFSISAIAYFFVGYYISYGHSFFQT
GAVLAADHGYNLMRCFFLLTFAAAIPAIISGGIAERAKMRPQAIATFALVALVYPLFEGMAWNGNFGLQKWLESQFGASF
HDFAGSVVVHAMGGWIALAAVMLLGARHGRYKKDGRVSAHPPSSIPFLALGSWILIVGWFGFNVMSAQRVDAISGLVAIN
SLMAMVGGTLSANFMGKNDPGFLHNGPLAGLVAICAGSDIVHPVSALVIGVIAGIIFVKLFTYTQNKLKIDDVLGVWPLH
GVCGALGGLAVGVFGQKAFGGLGGVSMMSQLIGTTFAIVIALVGGFVVYGILKATLGIRLSQEEEFQGADLSIHKISANS
EDGMF
>Mature_404_residues
GLSTFFEQIMQNVDLFFLLLGAVLVLAMHAGFAFLELGTVRHKNQVNALSKILTDFSISAIAYFFVGYYISYGHSFFQTG
AVLAADHGYNLMRCFFLLTFAAAIPAIISGGIAERAKMRPQAIATFALVALVYPLFEGMAWNGNFGLQKWLESQFGASFH
DFAGSVVVHAMGGWIALAAVMLLGARHGRYKKDGRVSAHPPSSIPFLALGSWILIVGWFGFNVMSAQRVDAISGLVAINS
LMAMVGGTLSANFMGKNDPGFLHNGPLAGLVAICAGSDIVHPVSALVIGVIAGIIFVKLFTYTQNKLKIDDVLGVWPLHG
VCGALGGLAVGVFGQKAFGGLGGVSMMSQLIGTTFAIVIALVGGFVVYGILKATLGIRLSQEEEFQGADLSIHKISANSE
DGMF

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=423, Percent_Identity=29.0780141843972, Blast_Score=137, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17550180, Length=427, Percent_Identity=30.4449648711944, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17550182, Length=427, Percent_Identity=28.8056206088993, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32564042, Length=432, Percent_Identity=27.3148148148148, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI133931100, Length=428, Percent_Identity=26.4018691588785, Blast_Score=99, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324187, Length=403, Percent_Identity=26.7990074441687, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=22.7272727272727, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325396, Length=349, Percent_Identity=23.2091690544413, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI161078314, Length=417, Percent_Identity=29.2565947242206, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42988; Mature: 42857

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
GLSTFFEQIMQNVDLFFLLLGAVLVLAMHAGFAFLELGTVRHKNQVNALSKILTDFSIS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIAYFFVGYYISYGHSFFQTGAVLAADHGYNLMRCFFLLTFAAAIPAIISGGIAERAKMR
HHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCC
PQAIATFALVALVYPLFEGMAWNGNFGLQKWLESQFGASFHDFAGSVVVHAMGGWIALAA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VMLLGARHGRYKKDGRVSAHPPSSIPFLALGSWILIVGWFGFNVMSAQRVDAISGLVAIN
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLMAMVGGTLSANFMGKNDPGFLHNGPLAGLVAICAGSDIVHPVSALVIGVIAGIIFVKL
HHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
FTYTQNKLKIDDVLGVWPLHGVCGALGGLAVGVFGQKAFGGLGGVSMMSQLIGTTFAIVI
HHHHCCCEEHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ALVGGFVVYGILKATLGIRLSQEEEFQGADLSIHKISANSEDGMF
HHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]