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The map label for this gene is ispB [H]

Identifier: 50085967

GI number: 50085967

Start: 2868306

End: 2869283

Strand: Direct

Name: ispB [H]

Synonym: ACIAD2940

Alternate gene names: 50085967

Gene position: 2868306-2869283 (Clockwise)

Preceding gene: 50085959

Following gene: 50085968

Centisome position: 79.71

GC content: 44.79

Gene sequence:

>978_bases
ATGGCCATCGACTTTAAGCAAGACATTCTCGCGCCTGTTGCTTCAGACTTCGCTGCGATGGATACATTTATTAATGAAGG
CATTACGTCCAAAGTTGCGCTGGTGATGGCCGTTAGTAAGCATGTTGTTGAGGCGGGTGGAAAGCGCATGCGTCCGATCA
TGTGCCTACTTGCTGCAAAAGCGTGTGGTTCAACTGATCTTGAACAGCATCGGAAGCTGGCCGCGATTATCGAAATGCTA
CATACCGCAACACTGGTACATGATGATGTGGTGGATGAATCAGGCTTGCGTCGTGGCCGCCCAACTGCAAATGCCACATG
GAATAATCAGACGGCAGTTTTAGTCGGTGATTTTCTGATCTCACGTGCTTTTGATTTACTGGTCGATTTGAATGACATGG
TGCTTCTGAAAGATTTCTCGACAGGTACCTGTGAGATTGCTGAAGGTGAAGTTTTACAATTACAGTCTCAGCATGACCCA
GAAACCACCGAACAGACTTATCTGAATATTATTCACGGTAAAACTTCGCGTCTGTTTGAACTTGCTACCGAAGGTGCTGC
AATTCTTTCTGGTACACCAGAATATCGTGAACCTCTACGACATTTTGCTGGACATTTTGGTAATGCATTCCAAATTATTG
ATGATATTTTGGACTATACCTCCGATGCAGAAACATTAGGTAAAAATATTGGTGATGATTTGATGGAAGGAAAGCCAACA
CTGCCATTAATCTCAGCATTACAACATACTCAAGGCGAAGAACATGATATTGTTCGTCGCAGTATTGCCACGGGTGGTAT
TCATCAGCTCGAACAAGTCATTCAGATTGTTCAGCAATCGGGTGCACTGGATTACTGCCGTAAACGTGCCGAAGAAGAAA
CCCAAGCCGCCTTGGCAGCGTTACAACATTTACCAGAAAGCGAAGCACGTCAGGCACTATTTAATCTTACCCAACTGGCT
TTGCACCGAATCCAATAA

Upstream 100 bases:

>100_bases
ATTTCTTTTTGAACACGATGATTCTACATATATAAGTCAATGATTGAATAGATCATGCGTAGTGACATTTATTTCATAGA
TTGATGAGGTTTCCCTTCAA

Downstream 100 bases:

>100_bases
ATATACTTAATCATCTGCCTATGCACATTCATCTCTCCGATCTTTATGCCAACATGCAAGAGCAGCTTGAGCATGCTCCC
ACTGCTTTAGATGGACAACT

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MAIDFKQDILAPVASDFAAMDTFINEGITSKVALVMAVSKHVVEAGGKRMRPIMCLLAAKACGSTDLEQHRKLAAIIEML
HTATLVHDDVVDESGLRRGRPTANATWNNQTAVLVGDFLISRAFDLLVDLNDMVLLKDFSTGTCEIAEGEVLQLQSQHDP
ETTEQTYLNIIHGKTSRLFELATEGAAILSGTPEYREPLRHFAGHFGNAFQIIDDILDYTSDAETLGKNIGDDLMEGKPT
LPLISALQHTQGEEHDIVRRSIATGGIHQLEQVIQIVQQSGALDYCRKRAEEETQAALAALQHLPESEARQALFNLTQLA
LHRIQ

Sequences:

>Translated_325_residues
MAIDFKQDILAPVASDFAAMDTFINEGITSKVALVMAVSKHVVEAGGKRMRPIMCLLAAKACGSTDLEQHRKLAAIIEML
HTATLVHDDVVDESGLRRGRPTANATWNNQTAVLVGDFLISRAFDLLVDLNDMVLLKDFSTGTCEIAEGEVLQLQSQHDP
ETTEQTYLNIIHGKTSRLFELATEGAAILSGTPEYREPLRHFAGHFGNAFQIIDDILDYTSDAETLGKNIGDDLMEGKPT
LPLISALQHTQGEEHDIVRRSIATGGIHQLEQVIQIVQQSGALDYCRKRAEEETQAALAALQHLPESEARQALFNLTQLA
LHRIQ
>Mature_324_residues
AIDFKQDILAPVASDFAAMDTFINEGITSKVALVMAVSKHVVEAGGKRMRPIMCLLAAKACGSTDLEQHRKLAAIIEMLH
TATLVHDDVVDESGLRRGRPTANATWNNQTAVLVGDFLISRAFDLLVDLNDMVLLKDFSTGTCEIAEGEVLQLQSQHDPE
TTEQTYLNIIHGKTSRLFELATEGAAILSGTPEYREPLRHFAGHFGNAFQIIDDILDYTSDAETLGKNIGDDLMEGKPTL
PLISALQHTQGEEHDIVRRSIATGGIHQLEQVIQIVQQSGALDYCRKRAEEETQAALAALQHLPESEARQALFNLTQLAL
HRIQ

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=297, Percent_Identity=31.6498316498317, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI4758430, Length=272, Percent_Identity=26.8382352941176, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI83700220, Length=272, Percent_Identity=26.8382352941176, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1789578, Length=312, Percent_Identity=47.1153846153846, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1786623, Length=274, Percent_Identity=29.9270072992701, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=310, Percent_Identity=28.7096774193548, Blast_Score=134, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6319475, Length=303, Percent_Identity=32.6732673267327, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325188, Length=258, Percent_Identity=25.1937984496124, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24651612, Length=296, Percent_Identity=29.0540540540541, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI281365769, Length=229, Percent_Identity=26.6375545851528, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24660002, Length=229, Percent_Identity=26.6375545851528, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35701; Mature: 35570

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDFKQDILAPVASDFAAMDTFINEGITSKVALVMAVSKHVVEAGGKRMRPIMCLLAAK
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ACGSTDLEQHRKLAAIIEMLHTATLVHDDVVDESGLRRGRPTANATWNNQTAVLVGDFLI
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCCCCEEHHHHHHHH
SRAFDLLVDLNDMVLLKDFSTGTCEIAEGEVLQLQSQHDPETTEQTYLNIIHGKTSRLFE
HHHHHHHHCCCCEEEEEECCCCCEEECCCCCEEECCCCCCCHHHHHHHHHHHCCHHHHHH
LATEGAAILSGTPEYREPLRHFAGHFGNAFQIIDDILDYTSDAETLGKNIGDDLMEGKPT
HHHCCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCHHHHCCCCC
LPLISALQHTQGEEHDIVRRSIATGGIHQLEQVIQIVQQSGALDYCRKRAEEETQAALAA
HHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LQHLPESEARQALFNLTQLALHRIQ
HHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIDFKQDILAPVASDFAAMDTFINEGITSKVALVMAVSKHVVEAGGKRMRPIMCLLAAK
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ACGSTDLEQHRKLAAIIEMLHTATLVHDDVVDESGLRRGRPTANATWNNQTAVLVGDFLI
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCCCCEEHHHHHHHH
SRAFDLLVDLNDMVLLKDFSTGTCEIAEGEVLQLQSQHDPETTEQTYLNIIHGKTSRLFE
HHHHHHHHCCCCEEEEEECCCCCEEECCCCCEEECCCCCCCHHHHHHHHHHHCCHHHHHH
LATEGAAILSGTPEYREPLRHFAGHFGNAFQIIDDILDYTSDAETLGKNIGDDLMEGKPT
HHHCCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCHHHHCCCCC
LPLISALQHTQGEEHDIVRRSIATGGIHQLEQVIQIVQQSGALDYCRKRAEEETQAALAA
HHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LQHLPESEARQALFNLTQLALHRIQ
HHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]