Click here to switch to the map view.

The map label for this gene is trmL [H]

Identifier: 50085960

GI number: 50085960

Start: 2862102

End: 2862581

Strand: Reverse

Name: trmL [H]

Synonym: ACIAD2933

Alternate gene names: 50085960

Gene position: 2862581-2862102 (Counterclockwise)

Preceding gene: 50085961

Following gene: 50085958

Centisome position: 79.55

GC content: 41.88

Gene sequence:

>480_bases
GTGATCCACGTTGTATTGTTTGAGCCTGAAATTCCTGCCAATACAGGCAATATTATCCGTTTGTGTGCCAATACTGGGGC
GCAATTGCATTTGGTTAAACCTTTGGGGTTTGAGCTAGATGATAAGAAACTCAAACGTGCAGGACTGGATTATCATGAAT
ATGCGCGGATGAAAATTTGGGATAATATTGAACAATGTCTTGAGTCTTTAAAATCACAAGGGATTTCTGAGTCCGATATT
TTTCCATTAACCACGAAGGGAAGTGCGACTCCGCATACTGTGGATTTAAATCGACCTGTTGCATTGCTGATGGGGCCTGA
AACGCGTGGTTTGCCTGAGTCTGTTCGGTTTATGTTTCCACAAAAAAACTGGATTCGTTTACCAATGGCAGAAAACTCTC
GTAGTTTGAACTTGTCTAATGCAACTGCGGTGATTGTATATGAAGCATGGCGACAACAGGGTTTTCAGCCTTTATCATGA

Upstream 100 bases:

>100_bases
GCGCCAACCTTAACCATCATTGGTGAAGTTGTCAGTTTACGTGAGCAGTTACAATGGTTATAAAACCAGCTGTTCTCTTA
TTTTGTAGTTGAGAAAATTT

Downstream 100 bases:

>100_bases
GTTGATCGACATTATTGCATCATATTGATGTTGGGCTTTTAGAACGATTTCAATATTCGATTCGGCCCATTCTTTAAGCT
TGAATGCAGTTTGTGAGACA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MIHVVLFEPEIPANTGNIIRLCANTGAQLHLVKPLGFELDDKKLKRAGLDYHEYARMKIWDNIEQCLESLKSQGISESDI
FPLTTKGSATPHTVDLNRPVALLMGPETRGLPESVRFMFPQKNWIRLPMAENSRSLNLSNATAVIVYEAWRQQGFQPLS

Sequences:

>Translated_159_residues
MIHVVLFEPEIPANTGNIIRLCANTGAQLHLVKPLGFELDDKKLKRAGLDYHEYARMKIWDNIEQCLESLKSQGISESDI
FPLTTKGSATPHTVDLNRPVALLMGPETRGLPESVRFMFPQKNWIRLPMAENSRSLNLSNATAVIVYEAWRQQGFQPLS
>Mature_159_residues
MIHVVLFEPEIPANTGNIIRLCANTGAQLHLVKPLGFELDDKKLKRAGLDYHEYARMKIWDNIEQCLESLKSQGISESDI
FPLTTKGSATPHTVDLNRPVALLMGPETRGLPESVRFMFPQKNWIRLPMAENSRSLNLSNATAVIVYEAWRQQGFQPLS

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=155, Percent_Identity=51.6129032258064, Blast_Score=165, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17944; Mature: 17944

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHVVLFEPEIPANTGNIIRLCANTGAQLHLVKPLGFELDDKKLKRAGLDYHEYARMKIW
CEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHH
DNIEQCLESLKSQGISESDIFPLTTKGSATPHTVDLNRPVALLMGPETRGLPESVRFMFP
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEECCCCEEEEECCCCCCCCCHHEEECC
QKNWIRLPMAENSRSLNLSNATAVIVYEAWRQQGFQPLS
CCCEEEEEECCCCCEEEECCCEEEEEEEHHHHCCCCCCC
>Mature Secondary Structure
MIHVVLFEPEIPANTGNIIRLCANTGAQLHLVKPLGFELDDKKLKRAGLDYHEYARMKIW
CEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHH
DNIEQCLESLKSQGISESDIFPLTTKGSATPHTVDLNRPVALLMGPETRGLPESVRFMFP
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEECCCCEEEEECCCCCCCCCHHEEECC
QKNWIRLPMAENSRSLNLSNATAVIVYEAWRQQGFQPLS
CCCEEEEEECCCCCEEEECCCEEEEEEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA