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The map label for this gene is rpoD [H]

Identifier: 50085952

GI number: 50085952

Start: 2854534

End: 2856423

Strand: Direct

Name: rpoD [H]

Synonym: ACIAD2925

Alternate gene names: 50085952

Gene position: 2854534-2856423 (Clockwise)

Preceding gene: 50085949

Following gene: 50085953

Centisome position: 79.32

GC content: 43.49

Gene sequence:

>1890_bases
ATGAGCGATATGCATTCCCCTACTTCTCAAGTAGCGGCTCTGATTAGCCGAGGCAAAGAACAAGGTTACTTAACCTACGC
TGAGGTTAACGATCATCTCCCAGACTCGATCACGGAAAGCGAGCAAATTGAAGACATCATTCAGATGCTTCAGGACGTTG
GTATTCCTGTGCATGAACGCGCACCTGAATCTGATGATACGATGTTTGGTGATTCCACAGAAGCCGATGAAGTTGCTGAA
GAAGAAGCAGCAGCAGTACTTGCATCTGTAGAAAATGAGCCAGGTCGTACTACAGATCCTGTACGCATGTATATGCGTGA
AATGGGAACGGTTGAGCTCTTAACGCGCGAAGGCGAAATCAGCATTGCAAAACGTATCGAAGAAGGTATTCGTGATGTTT
TGCATTCGATTGCTTACTGGCCGAATGCCGTTGAAGTTGTATTACAAGAATATAATGATTATGTAACAGGCGAACGTCGT
CTGGCCGATATTCTTTCAGGTTATCTCGATCCAGAAACAGATGATGATATTCCAGAAGTACTGGAAGAAGAGGCTGAACT
GGAAGAAGATGATGAATCTACATCTAAAACCACCACCAAAGACGTCAAACTTGATGATGATGACGAAGAAGAGGAAGAGT
CTGAAGGTGATGACGATTCTGAAGGTGATTCTGGTCCAGACCCTGAAATCGCAAAAGTACGTTTTGAAGAATTAGAAGCT
GCCTGGAAAAACACCAAGACTGTCATTGAAAAACACGGCCGCAATAGCGAAGAAGCAAATGCTGCGCTAGAGTCGCTCGC
AACTGTGTTTATGATGTTTAAATTTACCCCACGTTTGTTTGATATCATTTCAGAAATGATTCGTGGTACACATGAAAACA
TCCGTTCTAACGAACGTGAAGTGATGCGCTACGCAGTACGTCGTGGTCGTATGGATCGTACCCAGTTCCGTACCAGTTTC
CCAGGTCAAGAATCAAATCCGGCTTGGTTGGAAGAACAAATTGCGAAAGCGCCAAATGAAAGTAAGGCCTATCTTGAAAA
AGTACGTCCTGACATTCAGGCATTCCAGCAAAAAATTGCAGAGATTGAGCAAGACTTAAACCTAAATGTACGTGAAATTA
AAGAAATTGCAAAACGTATGGCTGTAGGCGAAGCCAAAGCTCGCCGTGCCAAAAAAGAGATGGTCGAAGCCAACTTGCGT
CTCGTTATTTCGATTGCGAAAAAGTATACTAACCGTGGTTTGCAATTCCTTGATTTGATTCAGGAAGGTAACATCGGCTT
GATGAAAGCAGTTGATAAATTTGAATACCGTCGTGGTTATAAATTTTCGACCTACGCAACGTGGTGGATTCGTCAGGCGA
TTACCCGCTCAATTGCGGATCAGGCACGTACCATCCGTATTCCGGTCCACATGATTGAAACGATTAACAAGATCAATCGC
GTATCACGTCAATTGTTACAAGAAATGGGGCGTGAACCAACACCAGAAGAGCTTGGTGAACGTCTGGAAATGGATGAAGT
CAAAGTTCGTAAAGTGTTGAAAATTGCCAAAGAGCCAATTTCAATGGAAACACCAATTGGTGATGATGAAGATTCTCATC
TTGGTGATTTTATTGAGGATTCAAACATCACTTCGCCAGTGGATGCAGCAACCTCAGAAGGCTTGAAAGAAGCAACACGT
GAAGTGCTTGAAAATCTGACCGAACGTGAAGCGAAAGTCTTGAAAATGCGTTTTGGTATCGATATGCCAACCGACCATAC
TTTGGAAGAAGTCGGTAAACAGTTTGATGTAACACGCGAACGTATTCGTCAGATCGAAGCCAAAGCCTTACGCAAATTAC
GTCATCCTTCGCGTTCTGAACACTTACGTTCATTCCTCGAAAACGACTAA

Upstream 100 bases:

>100_bases
TTAATTTATATGTAGCCGATCAAATCGAAAACATTCATAGCAAATTGTGCTATCATGCACGGTTTTTTACTGCCACAGAT
TCAACGAAGAGTAAGCAAAG

Downstream 100 bases:

>100_bases
GTTGAGACAGGGAAATGCCACTTGAAATTTTAGTGGCCAAACCCCATTTAATATTCAAAGACATTAATGCCTGCATCATC
GCAGGCATTAAATATGGAGA

Product: sigma D (sigma 70) factor of RNA polymerase, major sigma factor during exponential growth

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MSDMHSPTSQVAALISRGKEQGYLTYAEVNDHLPDSITESEQIEDIIQMLQDVGIPVHERAPESDDTMFGDSTEADEVAE
EEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREGEISIAKRIEEGIRDVLHSIAYWPNAVEVVLQEYNDYVTGERR
LADILSGYLDPETDDDIPEVLEEEAELEEDDESTSKTTTKDVKLDDDDEEEEESEGDDDSEGDSGPDPEIAKVRFEELEA
AWKNTKTVIEKHGRNSEEANAALESLATVFMMFKFTPRLFDIISEMIRGTHENIRSNEREVMRYAVRRGRMDRTQFRTSF
PGQESNPAWLEEQIAKAPNESKAYLEKVRPDIQAFQQKIAEIEQDLNLNVREIKEIAKRMAVGEAKARRAKKEMVEANLR
LVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINR
VSRQLLQEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDSNITSPVDAATSEGLKEATR
EVLENLTEREAKVLKMRFGIDMPTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEHLRSFLEND

Sequences:

>Translated_629_residues
MSDMHSPTSQVAALISRGKEQGYLTYAEVNDHLPDSITESEQIEDIIQMLQDVGIPVHERAPESDDTMFGDSTEADEVAE
EEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREGEISIAKRIEEGIRDVLHSIAYWPNAVEVVLQEYNDYVTGERR
LADILSGYLDPETDDDIPEVLEEEAELEEDDESTSKTTTKDVKLDDDDEEEEESEGDDDSEGDSGPDPEIAKVRFEELEA
AWKNTKTVIEKHGRNSEEANAALESLATVFMMFKFTPRLFDIISEMIRGTHENIRSNEREVMRYAVRRGRMDRTQFRTSF
PGQESNPAWLEEQIAKAPNESKAYLEKVRPDIQAFQQKIAEIEQDLNLNVREIKEIAKRMAVGEAKARRAKKEMVEANLR
LVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINR
VSRQLLQEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDSNITSPVDAATSEGLKEATR
EVLENLTEREAKVLKMRFGIDMPTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEHLRSFLEND
>Mature_628_residues
SDMHSPTSQVAALISRGKEQGYLTYAEVNDHLPDSITESEQIEDIIQMLQDVGIPVHERAPESDDTMFGDSTEADEVAEE
EAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREGEISIAKRIEEGIRDVLHSIAYWPNAVEVVLQEYNDYVTGERRL
ADILSGYLDPETDDDIPEVLEEEAELEEDDESTSKTTTKDVKLDDDDEEEEESEGDDDSEGDSGPDPEIAKVRFEELEAA
WKNTKTVIEKHGRNSEEANAALESLATVFMMFKFTPRLFDIISEMIRGTHENIRSNEREVMRYAVRRGRMDRTQFRTSFP
GQESNPAWLEEQIAKAPNESKAYLEKVRPDIQAFQQKIAEIEQDLNLNVREIKEIAKRMAVGEAKARRAKKEMVEANLRL
VISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINRV
SRQLLQEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDSNITSPVDAATSEGLKEATRE
VLENLTEREAKVLKMRFGIDMPTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEHLRSFLEND

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=625, Percent_Identity=59.68, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=271, Percent_Identity=39.8523985239852, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1789871, Length=252, Percent_Identity=27.3809523809524, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=29.2079207920792, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 71984; Mature: 71853

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMHSPTSQVAALISRGKEQGYLTYAEVNDHLPDSITESEQIEDIIQMLQDVGIPVHER
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
APESDDTMFGDSTEADEVAEEEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREGEI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCCCH
SIAKRIEEGIRDVLHSIAYWPNAVEVVLQEYNDYVTGERRLADILSGYLDPETDDDIPEV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHH
LEEEAELEEDDESTSKTTTKDVKLDDDDEEEEESEGDDDSEGDSGPDPEIAKVRFEELEA
HHHHHHCCCCCCHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
AWKNTKTVIEKHGRNSEEANAALESLATVFMMFKFTPRLFDIISEMIRGTHENIRSNERE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VMRYAVRRGRMDRTQFRTSFPGQESNPAWLEEQIAKAPNESKAYLEKVRPDIQAFQQKIA
HHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHH
EIEQDLNLNVREIKEIAKRMAVGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
QEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINR
HCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHH
VSRQLLQEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIED
HHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHC
SNITSPVDAATSEGLKEATREVLENLTEREAKVLKMRFGIDMPTDHTLEEVGKQFDVTRE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
RIRQIEAKALRKLRHPSRSEHLRSFLEND
HHHHHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SDMHSPTSQVAALISRGKEQGYLTYAEVNDHLPDSITESEQIEDIIQMLQDVGIPVHER
CCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
APESDDTMFGDSTEADEVAEEEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREGEI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCCCH
SIAKRIEEGIRDVLHSIAYWPNAVEVVLQEYNDYVTGERRLADILSGYLDPETDDDIPEV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHH
LEEEAELEEDDESTSKTTTKDVKLDDDDEEEEESEGDDDSEGDSGPDPEIAKVRFEELEA
HHHHHHCCCCCCHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
AWKNTKTVIEKHGRNSEEANAALESLATVFMMFKFTPRLFDIISEMIRGTHENIRSNERE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VMRYAVRRGRMDRTQFRTSFPGQESNPAWLEEQIAKAPNESKAYLEKVRPDIQAFQQKIA
HHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHH
EIEQDLNLNVREIKEIAKRMAVGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
QEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINR
HCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHH
VSRQLLQEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIED
HHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHC
SNITSPVDAATSEGLKEATREVLENLTEREAKVLKMRFGIDMPTDHTLEEVGKQFDVTRE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
RIRQIEAKALRKLRHPSRSEHLRSFLEND
HHHHHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665535 [H]