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The map label for this gene is nadE [H]

Identifier: 50085923

GI number: 50085923

Start: 2827405

End: 2829030

Strand: Direct

Name: nadE [H]

Synonym: ACIAD2888

Alternate gene names: 50085923

Gene position: 2827405-2829030 (Clockwise)

Preceding gene: 50085921

Following gene: 50085924

Centisome position: 78.57

GC content: 41.27

Gene sequence:

>1626_bases
ATGAAAAGTTTTAAAATTGCCCTTGCTCAATTTTCACCGCATATTGGCAATATTGATTCAAATGCTCAAAGAATGGTTGA
GCAAGCAAATGAAGCTAAAAAACAAAATGCTGACTTAATTATTTTTCCAGAACTTTCTGTGATTGGCTATCCCGCAGAAG
ATCTTCTTTTACGTCCAAATTTAAACAAACGGATGCAGAAGGCATTTCAGCAACTCAAGGAAGTTAAAGATATTGTCATG
GTGTTTGGGTTTGTTCATCAAACCGAAGAAGGTCATCGTTACAATTCTGCAGCTGTCATGAAAGATGGTGTTGTTCTGGG
TGTTTACAACAAACACAATTTACCAAACTATAGTGTGTTTGATGAAAAACGCTATTTTAGTCCTGGTCATCAGCACTTGG
TGTTTGAATACCTTGGACATAAATTTGGTGTTTTGATTTGTGAAGATATCTGGTCGATCAATACGGTTAAGCAACTCTCT
AAGTTGAATGTTGAAACTGTTCTGGTTCTAAACGCATCTCCTTATGAAGTAGGTAAACCACAGCATCGTGTCCAAACATT
GACTGAGCTATCTAAGCAGCTCAATGTTCATCTGGTTTATCTCAATCAAGTGGGTGGTCAGGATGATCTAATTTTTGATG
GTAGCAGCTTTATTATCAATCACGATGGAGAAGTCGCATTTCAGGCGCCAAGCTTCAAAGAAGAGCTGTACTACAGTGAA
TTTGATATTGAACAAAAGCGCTATAAAAAAATTGACCCTGCCCCCGCACTTGATACCATTGCTGAAATCTATCAAAGTTT
GGTCATGGCAACGCGTGACTACGTTCAACGCTCAGGCTTCTCAGGCGTAATTCTAGGTTTGTCTGGCGGTATCGATTCTG
CTTTAACGCTCGCCATCGCGGCCGATGCGATTGGTGCAGACAAAGTACAGGCCGTCATGATGCCATATACTTATACATCT
CAGATTAGTGTTGAAGATGCAACCGAACAAGCGCGTCGTATGGGTGTAACTTTCGGAATTGCCGAGATTCATCCAATCGT
CAATAGTTTTATGCAAACGCTCTACCCGTTCTTTGGAAATGCGCCTGCGGATGCAACTGAAGAAAACTTGCAAGCACGTG
CTCGTGGTACGTTGTTAATGGGATTGTCCAATAAATTTGGTAATTTAGTCTTATCAACAGGAAATAAATCTGAGCTTGCT
GTGGGTTATTGCACACTTTACGGTGACATGGTGGGTGGCTTTGCTGTTCTTAAGGATGTGTATAAAACCATCGTATTTGA
ACTCGCAAAATACAGAAACACTCTGAGTGAAACACCTGTTATTCCAGAACGTGTGATTACTCGTCCGCCATCGGCAGAAT
TGCGCCCCGATCAGAAAGATCAGGATTCGCTACCTGCTTATGATATTCTTGATGCAATTCTTTATGCTTATATTGAAGAA
GATCAAAGTCAGTCAGATATCATTGCCAAAGGCTTCGACAAAGAAGTCGTTGAAAAAGTGATTCGCCTGGTTGATCGAAA
TGAATATAAACGCCGTCAAGGTGCAATTGGCCCGCGTATTAGCTCACGTGCTTTTAGTCGAGAGCGTCGTTACCCTATCG
TAAATGGCTGGAGACCCGATGACTAA

Upstream 100 bases:

>100_bases
ACTTTTTTGTGTTATCCCATACGTCGAAACGTTGCATAATGACAACGACAAAATCAACTGCTGACGTATATCTGTGCTAT
CGATCTAGGACTAGGTTTGA

Downstream 100 bases:

>100_bases
GGATCAGACATCATCTAGCGAACAACTTGCTCAAGCACTTTTGCTTGAGCAAGTTCGTTATTATCAACAACAACTTTCTG
CAGATCACTCTCCCGAATAT

Product: glutamine-dependent NAD(+) synthetase (NAD(+) synthase [glutamine-hydrolyzing]) (NadE)

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MKSFKIALAQFSPHIGNIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLRPNLNKRMQKAFQQLKEVKDIVM
VFGFVHQTEEGHRYNSAAVMKDGVVLGVYNKHNLPNYSVFDEKRYFSPGHQHLVFEYLGHKFGVLICEDIWSINTVKQLS
KLNVETVLVLNASPYEVGKPQHRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHDGEVAFQAPSFKEELYYSE
FDIEQKRYKKIDPAPALDTIAEIYQSLVMATRDYVQRSGFSGVILGLSGGIDSALTLAIAADAIGADKVQAVMMPYTYTS
QISVEDATEQARRMGVTFGIAEIHPIVNSFMQTLYPFFGNAPADATEENLQARARGTLLMGLSNKFGNLVLSTGNKSELA
VGYCTLYGDMVGGFAVLKDVYKTIVFELAKYRNTLSETPVIPERVITRPPSAELRPDQKDQDSLPAYDILDAILYAYIEE
DQSQSDIIAKGFDKEVVEKVIRLVDRNEYKRRQGAIGPRISSRAFSRERRYPIVNGWRPDD

Sequences:

>Translated_541_residues
MKSFKIALAQFSPHIGNIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLRPNLNKRMQKAFQQLKEVKDIVM
VFGFVHQTEEGHRYNSAAVMKDGVVLGVYNKHNLPNYSVFDEKRYFSPGHQHLVFEYLGHKFGVLICEDIWSINTVKQLS
KLNVETVLVLNASPYEVGKPQHRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHDGEVAFQAPSFKEELYYSE
FDIEQKRYKKIDPAPALDTIAEIYQSLVMATRDYVQRSGFSGVILGLSGGIDSALTLAIAADAIGADKVQAVMMPYTYTS
QISVEDATEQARRMGVTFGIAEIHPIVNSFMQTLYPFFGNAPADATEENLQARARGTLLMGLSNKFGNLVLSTGNKSELA
VGYCTLYGDMVGGFAVLKDVYKTIVFELAKYRNTLSETPVIPERVITRPPSAELRPDQKDQDSLPAYDILDAILYAYIEE
DQSQSDIIAKGFDKEVVEKVIRLVDRNEYKRRQGAIGPRISSRAFSRERRYPIVNGWRPDD
>Mature_541_residues
MKSFKIALAQFSPHIGNIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLRPNLNKRMQKAFQQLKEVKDIVM
VFGFVHQTEEGHRYNSAAVMKDGVVLGVYNKHNLPNYSVFDEKRYFSPGHQHLVFEYLGHKFGVLICEDIWSINTVKQLS
KLNVETVLVLNASPYEVGKPQHRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHDGEVAFQAPSFKEELYYSE
FDIEQKRYKKIDPAPALDTIAEIYQSLVMATRDYVQRSGFSGVILGLSGGIDSALTLAIAADAIGADKVQAVMMPYTYTS
QISVEDATEQARRMGVTFGIAEIHPIVNSFMQTLYPFFGNAPADATEENLQARARGTLLMGLSNKFGNLVLSTGNKSELA
VGYCTLYGDMVGGFAVLKDVYKTIVFELAKYRNTLSETPVIPERVITRPPSAELRPDQKDQDSLPAYDILDAILYAYIEE
DQSQSDIIAKGFDKEVVEKVIRLVDRNEYKRRQGAIGPRISSRAFSRERRYPIVNGWRPDD

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60779; Mature: 60779

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFKIALAQFSPHIGNIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLRPN
CCCCEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEECCCHHHEEECCC
LNKRMQKAFQQLKEVKDIVMVFGFVHQTEEGHRYNSAAVMKDGVVLGVYNKHNLPNYSVF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
DEKRYFSPGHQHLVFEYLGHKFGVLICEDIWSINTVKQLSKLNVETVLVLNASPYEVGKP
CCCCCCCCCHHHHHHHHHCCHHCEEEEHHHHCHHHHHHHHHCCCEEEEEEECCCCCCCCH
QHRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHDGEVAFQAPSFKEELYYSE
HHHHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCEEEEECCCCEEEECCCHHHHHHHHH
FDIEQKRYKKIDPAPALDTIAEIYQSLVMATRDYVQRSGFSGVILGLSGGIDSALTLAIA
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
ADAIGADKVQAVMMPYTYTSQISVEDATEQARRMGVTFGIAEIHPIVNSFMQTLYPFFGN
HHHCCCHHHEEEEECEEECCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
APADATEENLQARARGTLLMGLSNKFGNLVLSTGNKSELAVGYCTLYGDMVGGFAVLKDV
CCCCCCHHHHHHHHCCEEEEECCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHH
YKTIVFELAKYRNTLSETPVIPERVITRPPSAELRPDQKDQDSLPAYDILDAILYAYIEE
HHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
DQSQSDIIAKGFDKEVVEKVIRLVDRNEYKRRQGAIGPRISSRAFSRERRYPIVNGWRPD
CCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCC
D
C
>Mature Secondary Structure
MKSFKIALAQFSPHIGNIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLRPN
CCCCEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEECCCHHHEEECCC
LNKRMQKAFQQLKEVKDIVMVFGFVHQTEEGHRYNSAAVMKDGVVLGVYNKHNLPNYSVF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
DEKRYFSPGHQHLVFEYLGHKFGVLICEDIWSINTVKQLSKLNVETVLVLNASPYEVGKP
CCCCCCCCCHHHHHHHHHCCHHCEEEEHHHHCHHHHHHHHHCCCEEEEEEECCCCCCCCH
QHRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHDGEVAFQAPSFKEELYYSE
HHHHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCEEEEECCCCEEEECCCHHHHHHHHH
FDIEQKRYKKIDPAPALDTIAEIYQSLVMATRDYVQRSGFSGVILGLSGGIDSALTLAIA
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
ADAIGADKVQAVMMPYTYTSQISVEDATEQARRMGVTFGIAEIHPIVNSFMQTLYPFFGN
HHHCCCHHHEEEEECEEECCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
APADATEENLQARARGTLLMGLSNKFGNLVLSTGNKSELAVGYCTLYGDMVGGFAVLKDV
CCCCCCHHHHHHHHCCEEEEECCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHH
YKTIVFELAKYRNTLSETPVIPERVITRPPSAELRPDQKDQDSLPAYDILDAILYAYIEE
HHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
DQSQSDIIAKGFDKEVVEKVIRLVDRNEYKRRQGAIGPRISSRAFSRERRYPIVNGWRPD
CCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]