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The map label for this gene is pckG [H]

Identifier: 50085885

GI number: 50085885

Start: 2779570

End: 2781399

Strand: Direct

Name: pckG [H]

Synonym: ACIAD2842

Alternate gene names: 50085885

Gene position: 2779570-2781399 (Clockwise)

Preceding gene: 50085880

Following gene: 50085886

Centisome position: 77.24

GC content: 44.86

Gene sequence:

>1830_bases
ATGACCCAAGTGAACGCACCTGAATTCGTTCGTCATCCTAAGCTTATTGCATGGGTTGAAGAAATTGCAAAATTGACCAA
ACCTGCAAAAATCGAGTGGTGTGACGGCAGCGACGAAGAATATCAACGTCTGATCGACTTGATGATCGCAAACGGCACAA
TGCAAGCCCTCAACCAAGAAAAACACCCAGGTTCTTATCTTGCAAATTCTGACCCTTCTGACGTTGCACGTGTTGAAGAC
CGTACATTTATCTGTTCTGAAAAACAAGAAGATGCTGGTGCAACCAACAACTGGGAAGCTCCAGAAGTCATGCGTGCCAA
GTTAAACGGTTTGTTTGATGGCTGTATGCAAGGTCGTACCATGTATGTGGTTCCATTCTCTATGGGTCCTTTGGGCAGTC
ACATCGCTCAAATTGGTATCGAATTGACTGACTCTCCATATGTGGCTGTGAGCATGCGTAAAATGGCACGTATGGGCAAA
GCGGTTTATGATGTGTTAGGCACTGATGGTGAATTTGTACCATGTGTACATACTGTTGCTGCACCTCTTGCAGAAGGTCA
AAAAGATGTTGCATGGCCATGCAACCCAGAAAAATATATCGTTCATTATCCTGAGACTCGTGAAATCTGGTCTTACGGTT
CTGGTTACGGCGGTAACGCATTACTTGGTAAAAAATGCTTGGCATTACGTATCGCATCTGCAATGGGTCGCGAGCAAGGC
TGGTTAGCTGAACACATGCTGATTCTGGGCGTAACCAATCCAAAAGGTGAAAAACACTACATCGCTGCAGCATTCCCATC
AGCTTGTGGTAAAACCAATTTTGCCATGCTCATTCCACCAGCAGGTTACGAAGGTTGGAAAATCGAAACTGTAGGTGACG
ATATCGCATGGATTAAACCTGGCGAAGATGGTCGCTTATATGCAATTAATCCAGAAGCTGGTTTCTTCGGTGTTGCGCCT
GGTACAAATACCAAAACCAACCCGAACTGCATGGCAACGCTTCACAAAGATGTGATTTATACCAACGTTGCTGTGACTGA
AGATGGTCAAGTATGGTGGGAAGGTCTTTCTAAAGAAGTTCCAGCTAACTTAACGAACTGGAAAGGTCAGCCACACGTTG
CGGGTGAAAAAGCTGCACATCCAAATGCACGTTTCACTGTAGCGGCAGGTCAATGCCCATCTATCGATGCAGACTGGGAA
AATCCAGCAGGCGTTCCAATTTCTGCTTTCATCTTTGGTGGTCGTCGTGCAGATACCGTTCCTTTGGTTTCTGAAGCATT
TGACTGGGTAGATGGCGTTTACAAAGCAGCGACTATGGGTTCTGAAACTACTGCCGCAGCTGTTGGTCAACAAGGTATTG
TACGCCGTGACCCATTTGCAATGTTACCTTTCGCGGGTTACAACATGGCAGATTACTTTGACCACTGGTTACAATTGGGT
CAACAAGTAGGCGAGAAAGCTGAAGCTGCGGGTAACAAATTACCAAAAATCTTCAATGTAAACTGGTTCCGTCGTGATGC
TGAAGGCAACTTTGTTTGGCCTGGTTTTGGTCAAAACATGCGTGTGCTTGAGTGGATGATTGATCGTGTTGAAGGTCGTG
CCAATGCCGTAGAAACACCTATTGGTTATGTCCCAACTTACGAAGATCTTAACTGGACAGGCACAGATTTTTCTAAAGAA
GAATTTGATCTGATCACCTCTCAAAGCAAAGATCAATGGATCACAGAAATTGAAAGCCATACCGAACTTTTCAACAAGTT
AGGTGAGCGTCTTCCAAAAGCGTTGAAAGAGCGTCAAGATCAGCTTTTACAAGCTGTTCAAAAAATCTAA

Upstream 100 bases:

>100_bases
AATTTATGCTTTAATAGCGCCACTTTTTTGACTTTATCTCTTTTGTTGTACCCCAACAAAGGGATTTGTACATTGTACAT
CTTTTAAAATAGGCCTCACC

Downstream 100 bases:

>100_bases
TTTAATTTGAATAAAATAAAGAGGCCATGTATGGCCTCTTTATTTTTTAGATTCTTTTTAAAAACATACTTATTTTCAAA
AAATATAACTTTTAATATCT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MTQVNAPEFVRHPKLIAWVEEIAKLTKPAKIEWCDGSDEEYQRLIDLMIANGTMQALNQEKHPGSYLANSDPSDVARVED
RTFICSEKQEDAGATNNWEAPEVMRAKLNGLFDGCMQGRTMYVVPFSMGPLGSHIAQIGIELTDSPYVAVSMRKMARMGK
AVYDVLGTDGEFVPCVHTVAAPLAEGQKDVAWPCNPEKYIVHYPETREIWSYGSGYGGNALLGKKCLALRIASAMGREQG
WLAEHMLILGVTNPKGEKHYIAAAFPSACGKTNFAMLIPPAGYEGWKIETVGDDIAWIKPGEDGRLYAINPEAGFFGVAP
GTNTKTNPNCMATLHKDVIYTNVAVTEDGQVWWEGLSKEVPANLTNWKGQPHVAGEKAAHPNARFTVAAGQCPSIDADWE
NPAGVPISAFIFGGRRADTVPLVSEAFDWVDGVYKAATMGSETTAAAVGQQGIVRRDPFAMLPFAGYNMADYFDHWLQLG
QQVGEKAEAAGNKLPKIFNVNWFRRDAEGNFVWPGFGQNMRVLEWMIDRVEGRANAVETPIGYVPTYEDLNWTGTDFSKE
EFDLITSQSKDQWITEIESHTELFNKLGERLPKALKERQDQLLQAVQKI

Sequences:

>Translated_609_residues
MTQVNAPEFVRHPKLIAWVEEIAKLTKPAKIEWCDGSDEEYQRLIDLMIANGTMQALNQEKHPGSYLANSDPSDVARVED
RTFICSEKQEDAGATNNWEAPEVMRAKLNGLFDGCMQGRTMYVVPFSMGPLGSHIAQIGIELTDSPYVAVSMRKMARMGK
AVYDVLGTDGEFVPCVHTVAAPLAEGQKDVAWPCNPEKYIVHYPETREIWSYGSGYGGNALLGKKCLALRIASAMGREQG
WLAEHMLILGVTNPKGEKHYIAAAFPSACGKTNFAMLIPPAGYEGWKIETVGDDIAWIKPGEDGRLYAINPEAGFFGVAP
GTNTKTNPNCMATLHKDVIYTNVAVTEDGQVWWEGLSKEVPANLTNWKGQPHVAGEKAAHPNARFTVAAGQCPSIDADWE
NPAGVPISAFIFGGRRADTVPLVSEAFDWVDGVYKAATMGSETTAAAVGQQGIVRRDPFAMLPFAGYNMADYFDHWLQLG
QQVGEKAEAAGNKLPKIFNVNWFRRDAEGNFVWPGFGQNMRVLEWMIDRVEGRANAVETPIGYVPTYEDLNWTGTDFSKE
EFDLITSQSKDQWITEIESHTELFNKLGERLPKALKERQDQLLQAVQKI
>Mature_608_residues
TQVNAPEFVRHPKLIAWVEEIAKLTKPAKIEWCDGSDEEYQRLIDLMIANGTMQALNQEKHPGSYLANSDPSDVARVEDR
TFICSEKQEDAGATNNWEAPEVMRAKLNGLFDGCMQGRTMYVVPFSMGPLGSHIAQIGIELTDSPYVAVSMRKMARMGKA
VYDVLGTDGEFVPCVHTVAAPLAEGQKDVAWPCNPEKYIVHYPETREIWSYGSGYGGNALLGKKCLALRIASAMGREQGW
LAEHMLILGVTNPKGEKHYIAAAFPSACGKTNFAMLIPPAGYEGWKIETVGDDIAWIKPGEDGRLYAINPEAGFFGVAPG
TNTKTNPNCMATLHKDVIYTNVAVTEDGQVWWEGLSKEVPANLTNWKGQPHVAGEKAAHPNARFTVAAGQCPSIDADWEN
PAGVPISAFIFGGRRADTVPLVSEAFDWVDGVYKAATMGSETTAAAVGQQGIVRRDPFAMLPFAGYNMADYFDHWLQLGQ
QVGEKAEAAGNKLPKIFNVNWFRRDAEGNFVWPGFGQNMRVLEWMIDRVEGRANAVETPIGYVPTYEDLNWTGTDFSKEE
FDLITSQSKDQWITEIESHTELFNKLGERLPKALKERQDQLLQAVQKI

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=618, Percent_Identity=50.9708737864078, Blast_Score=591, Evalue=1e-169,
Organism=Homo sapiens, GI66346721, Length=608, Percent_Identity=50, Blast_Score=584, Evalue=1e-167,
Organism=Homo sapiens, GI66346723, Length=387, Percent_Identity=51.421188630491, Blast_Score=391, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17555492, Length=625, Percent_Identity=48.96, Blast_Score=580, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI71989645, Length=614, Percent_Identity=47.8827361563518, Blast_Score=566, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71989658, Length=614, Percent_Identity=47.8827361563518, Blast_Score=565, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71989653, Length=601, Percent_Identity=48.2529118136439, Blast_Score=559, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI32564411, Length=563, Percent_Identity=50.7992895204263, Blast_Score=553, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI32564407, Length=532, Percent_Identity=51.8796992481203, Blast_Score=530, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI71989649, Length=482, Percent_Identity=51.8672199170125, Blast_Score=491, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI32564409, Length=378, Percent_Identity=49.4708994708995, Blast_Score=347, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17540956, Length=618, Percent_Identity=25.5663430420712, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24655085, Length=608, Percent_Identity=50, Blast_Score=585, Evalue=1e-167,
Organism=Drosophila melanogaster, GI24655082, Length=606, Percent_Identity=48.6798679867987, Blast_Score=569, Evalue=1e-162,
Organism=Drosophila melanogaster, GI161077226, Length=571, Percent_Identity=49.9124343257443, Blast_Score=546, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24655088, Length=512, Percent_Identity=52.1484375, Blast_Score=521, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 67307; Mature: 67176

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVNAPEFVRHPKLIAWVEEIAKLTKPAKIEWCDGSDEEYQRLIDLMIANGTMQALNQE
CCCCCCHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHCCC
KHPGSYLANSDPSDVARVEDRTFICSEKQEDAGATNNWEAPEVMRAKLNGLFDGCMQGRT
CCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
MYVVPFSMGPLGSHIAQIGIELTDSPYVAVSMRKMARMGKAVYDVLGTDGEFVPCVHTVA
EEEEEECCCCHHHHHHHHCEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHH
APLAEGQKDVAWPCNPEKYIVHYPETREIWSYGSGYGGNALLGKKCLALRIASAMGREQG
HHHHCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
WLAEHMLILGVTNPKGEKHYIAAAFPSACGKTNFAMLIPPAGYEGWKIETVGDDIAWIKP
HHHHEEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCEEEEECCCCEEEECC
GEDGRLYAINPEAGFFGVAPGTNTKTNPNCMATLHKDVIYTNVAVTEDGQVWWEGLSKEV
CCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHEEEEEEEEECCCHHHHHCCCCCC
PANLTNWKGQPHVAGEKAAHPNARFTVAAGQCPSIDADWENPAGVPISAFIFGGRRADTV
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCC
PLVSEAFDWVDGVYKAATMGSETTAAAVGQQGIVRRDPFAMLPFAGYNMADYFDHWLQLG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEECCCCEECCCCCCCHHHHHHHHHHHH
QQVGEKAEAAGNKLPKIFNVNWFRRDAEGNFVWPGFGQNMRVLEWMIDRVEGRANAVETP
HHHHHHHHHHCCCCCEEEECCEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
IGYVPTYEDLNWTGTDFSKEEFDLITSQSKDQWITEIESHTELFNKLGERLPKALKERQD
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLLQAVQKI
HHHHHHHCC
>Mature Secondary Structure 
TQVNAPEFVRHPKLIAWVEEIAKLTKPAKIEWCDGSDEEYQRLIDLMIANGTMQALNQE
CCCCCHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHCCC
KHPGSYLANSDPSDVARVEDRTFICSEKQEDAGATNNWEAPEVMRAKLNGLFDGCMQGRT
CCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
MYVVPFSMGPLGSHIAQIGIELTDSPYVAVSMRKMARMGKAVYDVLGTDGEFVPCVHTVA
EEEEEECCCCHHHHHHHHCEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHH
APLAEGQKDVAWPCNPEKYIVHYPETREIWSYGSGYGGNALLGKKCLALRIASAMGREQG
HHHHCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
WLAEHMLILGVTNPKGEKHYIAAAFPSACGKTNFAMLIPPAGYEGWKIETVGDDIAWIKP
HHHHEEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCEEEEECCCCEEEECC
GEDGRLYAINPEAGFFGVAPGTNTKTNPNCMATLHKDVIYTNVAVTEDGQVWWEGLSKEV
CCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHEEEEEEEEECCCHHHHHCCCCCC
PANLTNWKGQPHVAGEKAAHPNARFTVAAGQCPSIDADWENPAGVPISAFIFGGRRADTV
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCC
PLVSEAFDWVDGVYKAATMGSETTAAAVGQQGIVRRDPFAMLPFAGYNMADYFDHWLQLG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEECCCCEECCCCCCCHHHHHHHHHHHH
QQVGEKAEAAGNKLPKIFNVNWFRRDAEGNFVWPGFGQNMRVLEWMIDRVEGRANAVETP
HHHHHHHHHHCCCCCEEEECCEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
IGYVPTYEDLNWTGTDFSKEEFDLITSQSKDQWITEIESHTELFNKLGERLPKALKERQD
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLLQAVQKI
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA