Click here to switch to the map view.

The map label for this gene is yjbJ [H]

Identifier: 50085875

GI number: 50085875

Start: 2767146

End: 2767997

Strand: Direct

Name: yjbJ [H]

Synonym: ACIAD2832

Alternate gene names: 50085875

Gene position: 2767146-2767997 (Clockwise)

Preceding gene: 50085872

Following gene: 50085876

Centisome position: 76.89

GC content: 42.49

Gene sequence:

>852_bases
ATGAAAAAACGCAAACACCTTTCTTTACATTTTATTTTGGCTTCAACACTGTCCTGTACCGCCATGAGCCATGTTTTTGC
TGGGCAAATTTTTGTCTATAAAGATAAATCTGGGGGAACTTTACTGACCAATCGTAAAAGTAGTGACCCATCAATGACCA
AGGTCAAAGCGACTTATTATCCAGATAGCAACATCCACAGTTATAATAACTGGGGTGCTTCCGAAGCATCGGTTTTGCCA
AGCTATAGCAAAAATCGTAATGCGTTTGACCAGATTATTCATCAAGCTTCACAGCGGCATGGCATTTCAGAAGGTCTGAT
TAAGGCAGTCATGCATACCGAATCTGGCTTTAATGTTTATGCGCGCTCGCCTGTAGGTGCACAAGGCCTCATGCAGCTTA
TGCCTGCCACAGCCAGACGTTTTAATGTCTCAAATGCCTATGATCCACAGGAAAATATTTTTGGAGGCGCACAATACTTA
AGCTGGTTACTCAAACGTTTTAATGGTAATACGCAGCTTGCCTTAGCCGCCTATAACGCAGGCGAAGGCAATGTCGATAA
ATATGGTGGTATCCCGCCTTTTCGGGAAACACAGGATTATGTACGCCGAGTCACCAGTCGCTATCAAAATCTTTACGCCA
GTGGTAACACTGTAAGTGAAAATCTAGCTACACCACAGGTCATTGCTCAATCTGCCAATTATGCTCCAGCGAATAATCAT
GTACCCGAGACTACCAAGTATTCCTCACAACGGCAAATTATCAGTTTAAGTGAAGGAACTTATACCGATGCACCAAATAC
CTATGTCACACGTAATGCAACAGCATCTGCCAGAATTCATATTTCAAATTAA

Upstream 100 bases:

>100_bases
AAACAAGTTTTAAATTTTTTAGGCATAAAACTCCCCTTTTAACGTTTTTTTGCTAAACTGCTGAACAAGATGTCACCCCC
TTACCAGCGAATAACATAAA

Downstream 100 bases:

>100_bases
TACCGATTAAAAACCCCTACATGATTTAACTTAAAAAAAGCTTAAATCAATTAATTTGTCTTGAATTTTGGCAAGAATCC
CCCCATATTTAACGCAATGA

Product: transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKKRKHLSLHFILASTLSCTAMSHVFAGQIFVYKDKSGGTLLTNRKSSDPSMTKVKATYYPDSNIHSYNNWGASEASVLP
SYSKNRNAFDQIIHQASQRHGISEGLIKAVMHTESGFNVYARSPVGAQGLMQLMPATARRFNVSNAYDPQENIFGGAQYL
SWLLKRFNGNTQLALAAYNAGEGNVDKYGGIPPFRETQDYVRRVTSRYQNLYASGNTVSENLATPQVIAQSANYAPANNH
VPETTKYSSQRQIISLSEGTYTDAPNTYVTRNATASARIHISN

Sequences:

>Translated_283_residues
MKKRKHLSLHFILASTLSCTAMSHVFAGQIFVYKDKSGGTLLTNRKSSDPSMTKVKATYYPDSNIHSYNNWGASEASVLP
SYSKNRNAFDQIIHQASQRHGISEGLIKAVMHTESGFNVYARSPVGAQGLMQLMPATARRFNVSNAYDPQENIFGGAQYL
SWLLKRFNGNTQLALAAYNAGEGNVDKYGGIPPFRETQDYVRRVTSRYQNLYASGNTVSENLATPQVIAQSANYAPANNH
VPETTKYSSQRQIISLSEGTYTDAPNTYVTRNATASARIHISN
>Mature_283_residues
MKKRKHLSLHFILASTLSCTAMSHVFAGQIFVYKDKSGGTLLTNRKSSDPSMTKVKATYYPDSNIHSYNNWGASEASVLP
SYSKNRNAFDQIIHQASQRHGISEGLIKAVMHTESGFNVYARSPVGAQGLMQLMPATARRFNVSNAYDPQENIFGGAQYL
SWLLKRFNGNTQLALAAYNAGEGNVDKYGGIPPFRETQDYVRRVTSRYQNLYASGNTVSENLATPQVIAQSANYAPANNH
VPETTKYSSQRQIISLSEGTYTDAPNTYVTRNATASARIHISN

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=142, Percent_Identity=31.6901408450704, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 31248; Mature: 31248

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRKHLSLHFILASTLSCTAMSHVFAGQIFVYKDKSGGTLLTNRKSSDPSMTKVKATYY
CCCCCCEEEEEEEHHHHHHHHHHHHHHCEEEEEEECCCCEEEECCCCCCCCCEEEEEEEC
PDSNIHSYNNWGASEASVLPSYSKNRNAFDQIIHQASQRHGISEGLIKAVMHTESGFNVY
CCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEE
ARSPVGAQGLMQLMPATARRFNVSNAYDPQENIFGGAQYLSWLLKRFNGNTQLALAAYNA
ECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHCCCCEEEEEEECC
GEGNVDKYGGIPPFRETQDYVRRVTSRYQNLYASGNTVSENLATPQVIAQSANYAPANNH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHCCCCCCCCCC
VPETTKYSSQRQIISLSEGTYTDAPNTYVTRNATASARIHISN
CCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCEEEEEECC
>Mature Secondary Structure
MKKRKHLSLHFILASTLSCTAMSHVFAGQIFVYKDKSGGTLLTNRKSSDPSMTKVKATYY
CCCCCCEEEEEEEHHHHHHHHHHHHHHCEEEEEEECCCCEEEECCCCCCCCCEEEEEEEC
PDSNIHSYNNWGASEASVLPSYSKNRNAFDQIIHQASQRHGISEGLIKAVMHTESGFNVY
CCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEE
ARSPVGAQGLMQLMPATARRFNVSNAYDPQENIFGGAQYLSWLLKRFNGNTQLALAAYNA
ECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHCCCCEEEEEEECC
GEGNVDKYGGIPPFRETQDYVRRVTSRYQNLYASGNTVSENLATPQVIAQSANYAPANNH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHCCCCCCCCCC
VPETTKYSSQRQIISLSEGTYTDAPNTYVTRNATASARIHISN
CCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]