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The map label for this gene is entB [H]

Identifier: 50085831

GI number: 50085831

Start: 2722126

End: 2722755

Strand: Reverse

Name: entB [H]

Synonym: ACIAD2775

Alternate gene names: 50085831

Gene position: 2722755-2722126 (Counterclockwise)

Preceding gene: 50085832

Following gene: 50085830

Centisome position: 75.66

GC content: 36.98

Gene sequence:

>630_bases
ATGACCATTCCAAAAATTCCAAGTTATCCACTTCCATTTAAACAAGATTATCCCGCTGCAAAAGTGAATTGGAAAATTGA
GAAAAATCGTGCTGCTTTATTGATTCATGACATGCAAGATTATTTTGTCAATTTCTACGGTGAAGATTCAGAATTGATTC
AACAGGTGGTTGATAACATCGCACAAATAAAAGCGTGGTGTGTAGCTAATGATGTACCAGTGTTTTATACCGCACAACCT
GCTGAACAAAAAGATCAAGATCGTGCTTTGTTGAATGATATGTGGGGGCCTGGTCTAACGGCTTATCCTGAGCAGGCCGC
AATTGTCAAAAAACTAATGCCAAATGAGCAAGATCAAGTGCTTACTAAATGGCGATATAGCGCTTTTTATAATTCAGACT
TAGAAAAAATGCTTAAAGACACTCAAAAAGATCAACTTATTATTTGTGGTGTTTATGCGCATATTGGTGTGCTTCAAACT
GCTGCAGAAGCCTTTATGAATGGTATTCAACCTTTTGTGGTCGGTGATTCAGTTGCTGACTTTAGTTTAAAAGATCATTT
GTTCGCTTTGCAATATGTTCAGAATAACTTGGGTGTTGTCGAAGATGTTGAATGTGTCATTGCTTCATAA

Upstream 100 bases:

>100_bases
TTCCTGATGAAAACAAATTCTATATTTGGCGCCAATAGAGCATGAAAACCAAATCAAAGACATATTTTTGAAAACAGATT
AACGATATATAGGCAACACT

Downstream 100 bases:

>100_bases
GGCTAAGAATTAACAGCTAACTCGAGGATGACCATGACACAGATCAATTCAGAAGTTTTAGATATAAATCTAGTAAAGAA
AATTGCGTGGGTAACTGGTG

Product: 2,3-dihydro-2,3-dihydroxybenzoate synthetase, isochorismatase

Products: NA

Alternate protein names: 2,3 dihydro-2,3 dihydroxybenzoate synthase; Enterobactin synthase component B; Enterochelin synthase B; Isochorismate lyase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTIPKIPSYPLPFKQDYPAAKVNWKIEKNRAALLIHDMQDYFVNFYGEDSELIQQVVDNIAQIKAWCVANDVPVFYTAQP
AEQKDQDRALLNDMWGPGLTAYPEQAAIVKKLMPNEQDQVLTKWRYSAFYNSDLEKMLKDTQKDQLIICGVYAHIGVLQT
AAEAFMNGIQPFVVGDSVADFSLKDHLFALQYVQNNLGVVEDVECVIAS

Sequences:

>Translated_209_residues
MTIPKIPSYPLPFKQDYPAAKVNWKIEKNRAALLIHDMQDYFVNFYGEDSELIQQVVDNIAQIKAWCVANDVPVFYTAQP
AEQKDQDRALLNDMWGPGLTAYPEQAAIVKKLMPNEQDQVLTKWRYSAFYNSDLEKMLKDTQKDQLIICGVYAHIGVLQT
AAEAFMNGIQPFVVGDSVADFSLKDHLFALQYVQNNLGVVEDVECVIAS
>Mature_208_residues
TIPKIPSYPLPFKQDYPAAKVNWKIEKNRAALLIHDMQDYFVNFYGEDSELIQQVVDNIAQIKAWCVANDVPVFYTAQPA
EQKDQDRALLNDMWGPGLTAYPEQAAIVKKLMPNEQDQVLTKWRYSAFYNSDLEKMLKDTQKDQLIICGVYAHIGVLQTA
AEAFMNGIQPFVVGDSVADFSLKDHLFALQYVQNNLGVVEDVECVIAS

Specific function: Required for production of 2,3-DHB. Also serves as an aryl carrier protein and plays a role in enterobactin assembly [H]

COG id: COG1535

COG function: function code Q; Isochorismate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=200, Percent_Identity=57, Blast_Score=252, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009081
- InterPro:   IPR016291
- InterPro:   IPR000868
- InterPro:   IPR006163 [H]

Pfam domain/function: PF00857 Isochorismatase; PF00550 PP-binding [H]

EC number: =3.3.2.1 [H]

Molecular weight: Translated: 23683; Mature: 23552

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPKIPSYPLPFKQDYPAAKVNWKIEKNRAALLIHDMQDYFVNFYGEDSELIQQVVDNI
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHHHCCCCHHHHHHHHHHH
AQIKAWCVANDVPVFYTAQPAEQKDQDRALLNDMWGPGLTAYPEQAAIVKKLMPNEQDQV
HHHHHHHEECCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHH
LTKWRYSAFYNSDLEKMLKDTQKDQLIICGVYAHIGVLQTAAEAFMNGIQPFVVGDSVAD
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
FSLKDHLFALQYVQNNLGVVEDVECVIAS
CHHHHHHHHHHHHHHCCCCCCCHHEEECC
>Mature Secondary Structure 
TIPKIPSYPLPFKQDYPAAKVNWKIEKNRAALLIHDMQDYFVNFYGEDSELIQQVVDNI
CCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHHHCCCCHHHHHHHHHHH
AQIKAWCVANDVPVFYTAQPAEQKDQDRALLNDMWGPGLTAYPEQAAIVKKLMPNEQDQV
HHHHHHHEECCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHH
LTKWRYSAFYNSDLEKMLKDTQKDQLIICGVYAHIGVLQTAAEAFMNGIQPFVVGDSVAD
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
FSLKDHLFALQYVQNNLGVVEDVECVIAS
CHHHHHHHHHHHHHHCCCCCCCHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]