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The map label for this gene is entE [H]

Identifier: 50085826

GI number: 50085826

Start: 2710709

End: 2712328

Strand: Reverse

Name: entE [H]

Synonym: ACIAD2770

Alternate gene names: 50085826

Gene position: 2712328-2710709 (Counterclockwise)

Preceding gene: 50085827

Following gene: 50085825

Centisome position: 75.37

GC content: 37.78

Gene sequence:

>1620_bases
ATGTTTTTAGAACAAGATATTCAACAAGGCTATGTTCCATTTCCTGAATCGAGAGCCAAACAGTATCGTGCAGAAGGTTG
TTGGAAATCGAATACCCATTTTCAAATGCTAGCAGATTCAGTTAGTCGTTTTGGTGACTGTATTGCGGCTATTCAAGACC
AAAAACAACTGACCTATAAACAATTGTATGACTATGCCATTCGCTACGGCGCATACTTAAAACATAAAGGTATTCGAGAA
ACAGACTTTGTTTTATTGCAATCACCCAATGTGATTGAAGTGTTTATTGTTATTTTTGGTTTATATGCCATTGGTGCAAG
ACCAGTCTTTTGTTTGCATGGTCATGGCGCTTATGAAATTGAAAATATTGCACGGCAAAGTCGTGCAGTAGGGTTTATCA
AATTATGTGGTACGGCTAATGAAATCACTGCAGTCGATGTTTGTGAAGAATTTTCTGTGCCGAACTTTCAGCTTTGGTTT
AGAGAAACGATATTGAGTCAAGAGACTATTGCAGGCTCGTTACCTCAGTTAAAACATCTCAACTTAGATACCAATATTCA
AGCTTTAAGTTCTTCAGAAGACATTGCATTTTTACAACTGTCAGGGGGGACAACGGGTTTACCAAAACTGATTCCACGCA
CTCATGCTGATTATATTTATTCGATTGAAAAAAGTGTCGAGGTTGCAGGTTTAAATCCACAGACCAAGCAACTGGTGGTG
TTACCTGTAATGCACAACTTTTGTATGAGTTCACCTGGTTTTCTTGGTGTGTTCTATGCAGGTGGAACGGTGGTGCTGAG
TCAATTAACACAACCCCGAGTTTGTTTTGAGCTGATCCAAAAATACAAGATTGAACAGGTGTCTTTGGTGCCTGCAATTG
CTACTTTATGGCTCAATGCCGAAAGTCTAAAAGACTACGATCTCTCTAGTTTAAAGGTGATTCAAGTAGGTGGAGCGAAG
CTTTTACCATCACTTGCTGAGCAAATTATTGATCAGTTCCAAGTCAAATTACAACAAGTGTATGGCATGGCTGAAGGTTT
AGTAAATTTCACTCATTTAGATGATACCAATGCGGTGACTATTCAAACCCAGGGTAAGAAGCTGTCACATTTAGATGAAA
TACGCATTACTGATCATCATGGACAAGCTTTGCCAATCAATGCGATTGGTCATATTCAAACCCGTGGTCCGTACACTATT
AATGGCTATTATAACTTGCATGAAATTAATCGCCGTTCATTTACTGATGATGGTTTTTATAAAACAGGAGATATCGGCTA
TTTAGATGATGACCTCAATATTGTCGTGACTGGACGTGAGAAAGAGCAGATTAATCGTTCTGGAGAAAAAGTCACACCCA
GCGAAATTGAAGAATTCATTTTGCAATATCCAACTGTTAAAGATGTCTGCGTGATTGGTGTCAGCGATGAACATTTGGGT
GAACGGATTAAAGCCATCATCATTCCTAAACATGAAGATCATGAAATTAGTTTAAAAAATATTCGTAAGTTTTTAACAGG
TAAAAATATTGCGCATTTTAAAATTCCAGATGAAATAGACGTTGTAGCGGATTTTAAATATACCCATGTTGGTAAAGTAA
ATAGACAAAAACTGGGATAA

Upstream 100 bases:

>100_bases
TACGCAGTCAGAAGAATGCACGCAGATTGATGTCATGGAAATCCGTCATCAGCTGTCAACCATTCAACTTCCAGAATTGG
CAATTTAAAGGATTCAGTTC

Downstream 100 bases:

>100_bases
GAAGGAATTAAAAATGAGTACACAACAGATTTATGACATTGTAGGTGTCGGTGTAGGGCCATTTAATTTAGGGTTAGCTT
GTCTCACAGCTCCATTATCA

Product: 2,3-dihydroxybenzoate-AMP ligase

Products: NA

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MFLEQDIQQGYVPFPESRAKQYRAEGCWKSNTHFQMLADSVSRFGDCIAAIQDQKQLTYKQLYDYAIRYGAYLKHKGIRE
TDFVLLQSPNVIEVFIVIFGLYAIGARPVFCLHGHGAYEIENIARQSRAVGFIKLCGTANEITAVDVCEEFSVPNFQLWF
RETILSQETIAGSLPQLKHLNLDTNIQALSSSEDIAFLQLSGGTTGLPKLIPRTHADYIYSIEKSVEVAGLNPQTKQLVV
LPVMHNFCMSSPGFLGVFYAGGTVVLSQLTQPRVCFELIQKYKIEQVSLVPAIATLWLNAESLKDYDLSSLKVIQVGGAK
LLPSLAEQIIDQFQVKLQQVYGMAEGLVNFTHLDDTNAVTIQTQGKKLSHLDEIRITDHHGQALPINAIGHIQTRGPYTI
NGYYNLHEINRRSFTDDGFYKTGDIGYLDDDLNIVVTGREKEQINRSGEKVTPSEIEEFILQYPTVKDVCVIGVSDEHLG
ERIKAIIIPKHEDHEISLKNIRKFLTGKNIAHFKIPDEIDVVADFKYTHVGKVNRQKLG

Sequences:

>Translated_539_residues
MFLEQDIQQGYVPFPESRAKQYRAEGCWKSNTHFQMLADSVSRFGDCIAAIQDQKQLTYKQLYDYAIRYGAYLKHKGIRE
TDFVLLQSPNVIEVFIVIFGLYAIGARPVFCLHGHGAYEIENIARQSRAVGFIKLCGTANEITAVDVCEEFSVPNFQLWF
RETILSQETIAGSLPQLKHLNLDTNIQALSSSEDIAFLQLSGGTTGLPKLIPRTHADYIYSIEKSVEVAGLNPQTKQLVV
LPVMHNFCMSSPGFLGVFYAGGTVVLSQLTQPRVCFELIQKYKIEQVSLVPAIATLWLNAESLKDYDLSSLKVIQVGGAK
LLPSLAEQIIDQFQVKLQQVYGMAEGLVNFTHLDDTNAVTIQTQGKKLSHLDEIRITDHHGQALPINAIGHIQTRGPYTI
NGYYNLHEINRRSFTDDGFYKTGDIGYLDDDLNIVVTGREKEQINRSGEKVTPSEIEEFILQYPTVKDVCVIGVSDEHLG
ERIKAIIIPKHEDHEISLKNIRKFLTGKNIAHFKIPDEIDVVADFKYTHVGKVNRQKLG
>Mature_539_residues
MFLEQDIQQGYVPFPESRAKQYRAEGCWKSNTHFQMLADSVSRFGDCIAAIQDQKQLTYKQLYDYAIRYGAYLKHKGIRE
TDFVLLQSPNVIEVFIVIFGLYAIGARPVFCLHGHGAYEIENIARQSRAVGFIKLCGTANEITAVDVCEEFSVPNFQLWF
RETILSQETIAGSLPQLKHLNLDTNIQALSSSEDIAFLQLSGGTTGLPKLIPRTHADYIYSIEKSVEVAGLNPQTKQLVV
LPVMHNFCMSSPGFLGVFYAGGTVVLSQLTQPRVCFELIQKYKIEQVSLVPAIATLWLNAESLKDYDLSSLKVIQVGGAK
LLPSLAEQIIDQFQVKLQQVYGMAEGLVNFTHLDDTNAVTIQTQGKKLSHLDEIRITDHHGQALPINAIGHIQTRGPYTI
NGYYNLHEINRRSFTDDGFYKTGDIGYLDDDLNIVVTGREKEQINRSGEKVTPSEIEEFILQYPTVKDVCVIGVSDEHLG
ERIKAIIIPKHEDHEISLKNIRKFLTGKNIAHFKIPDEIDVVADFKYTHVGKVNRQKLG

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=549, Percent_Identity=26.4116575591985, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI115511026, Length=510, Percent_Identity=23.1372549019608, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI38505220, Length=483, Percent_Identity=23.1884057971014, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI157311624, Length=483, Percent_Identity=24.0165631469979, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI157311622, Length=483, Percent_Identity=24.0165631469979, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI58082049, Length=484, Percent_Identity=24.1735537190083, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI187761345, Length=378, Percent_Identity=22.7513227513227, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI187761343, Length=378, Percent_Identity=22.7513227513227, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI42544132, Length=391, Percent_Identity=23.0179028132992, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI122937307, Length=265, Percent_Identity=26.0377358490566, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI28416953, Length=532, Percent_Identity=24.6240601503759, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1786810, Length=538, Percent_Identity=38.4758364312268, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI145693145, Length=531, Percent_Identity=27.3069679849341, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1788107, Length=539, Percent_Identity=24.6753246753247, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI221142682, Length=473, Percent_Identity=26.8498942917548, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789201, Length=529, Percent_Identity=20.9829867674858, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1786529, Length=532, Percent_Identity=22.3684210526316, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=515, Percent_Identity=27.5728155339806, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=510, Percent_Identity=27.4509803921569, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=375, Percent_Identity=30.9333333333333, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=396, Percent_Identity=27.7777777777778, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=373, Percent_Identity=31.3672922252011, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71994703, Length=372, Percent_Identity=26.6129032258064, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=373, Percent_Identity=27.3458445040214, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71994694, Length=372, Percent_Identity=26.6129032258064, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32563687, Length=501, Percent_Identity=25.5489021956088, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=532, Percent_Identity=26.3157894736842, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=507, Percent_Identity=23.8658777120316, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=369, Percent_Identity=25.4742547425474, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560140, Length=307, Percent_Identity=28.3387622149837, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=385, Percent_Identity=27.5324675324675, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6319264, Length=534, Percent_Identity=21.9101123595506, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581924, Length=551, Percent_Identity=24.6823956442831, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI18859661, Length=528, Percent_Identity=23.8636363636364, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21355181, Length=492, Percent_Identity=24.390243902439, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356947, Length=213, Percent_Identity=29.5774647887324, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648255, Length=488, Percent_Identity=22.7459016393443, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922652, Length=298, Percent_Identity=26.1744966442953, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648253, Length=342, Percent_Identity=24.2690058479532, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI281365686, Length=482, Percent_Identity=23.2365145228216, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24653035, Length=306, Percent_Identity=28.1045751633987, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20130357, Length=547, Percent_Identity=22.8519195612431, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356441, Length=341, Percent_Identity=24.3401759530792, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648260, Length=513, Percent_Identity=22.6120857699805, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355225, Length=578, Percent_Identity=21.9723183391003, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21358303, Length=333, Percent_Identity=24.3243243243243, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161076582, Length=459, Percent_Identity=23.3115468409586, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648257, Length=504, Percent_Identity=21.8253968253968, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24656500, Length=356, Percent_Identity=23.5955056179775, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.-

Molecular weight: Translated: 60582; Mature: 60582

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLEQDIQQGYVPFPESRAKQYRAEGCWKSNTHFQMLADSVSRFGDCIAAIQDQKQLTYK
CCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYDYAIRYGAYLKHKGIRETDFVLLQSPNVIEVFIVIFGLYAIGARPVFCLHGHGAYEI
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHH
ENIARQSRAVGFIKLCGTANEITAVDVCEEFSVPNFQLWFRETILSQETIAGSLPQLKHL
HHHHHHHHCEEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEC
NLDTNIQALSSSEDIAFLQLSGGTTGLPKLIPRTHADYIYSIEKSVEVAGLNPQTKQLVV
CCCCCCEEECCCCCEEEEEECCCCCCCCHHCCCCCCHHEEEECCCEEEECCCCCCCEEEE
LPVMHNFCMSSPGFLGVFYAGGTVVLSQLTQPRVCFELIQKYKIEQVSLVPAIATLWLNA
EEHHHHHHCCCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCH
ESLKDYDLSSLKVIQVGGAKLLPSLAEQIIDQFQVKLQQVYGMAEGLVNFTHLDDTNAVT
HHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEE
IQTQGKKLSHLDEIRITDHHGQALPINAIGHIQTRGPYTINGYYNLHEINRRSFTDDGFY
EEECCCCCCCCHHEEEECCCCCEEEECCCEEEECCCCEEEECEEEEEECCCCCCCCCCCE
KTGDIGYLDDDLNIVVTGREKEQINRSGEKVTPSEIEEFILQYPTVKDVCVIGVSDEHLG
ECCCCCEECCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHC
ERIKAIIIPKHEDHEISLKNIRKFLTGKNIAHFKIPDEIDVVADFKYTHVGKVNRQKLG
CCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCEEECCCCCHHCCC
>Mature Secondary Structure
MFLEQDIQQGYVPFPESRAKQYRAEGCWKSNTHFQMLADSVSRFGDCIAAIQDQKQLTYK
CCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYDYAIRYGAYLKHKGIRETDFVLLQSPNVIEVFIVIFGLYAIGARPVFCLHGHGAYEI
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHH
ENIARQSRAVGFIKLCGTANEITAVDVCEEFSVPNFQLWFRETILSQETIAGSLPQLKHL
HHHHHHHHCEEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEC
NLDTNIQALSSSEDIAFLQLSGGTTGLPKLIPRTHADYIYSIEKSVEVAGLNPQTKQLVV
CCCCCCEEECCCCCEEEEEECCCCCCCCHHCCCCCCHHEEEECCCEEEECCCCCCCEEEE
LPVMHNFCMSSPGFLGVFYAGGTVVLSQLTQPRVCFELIQKYKIEQVSLVPAIATLWLNA
EEHHHHHHCCCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCH
ESLKDYDLSSLKVIQVGGAKLLPSLAEQIIDQFQVKLQQVYGMAEGLVNFTHLDDTNAVT
HHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEE
IQTQGKKLSHLDEIRITDHHGQALPINAIGHIQTRGPYTINGYYNLHEINRRSFTDDGFY
EEECCCCCCCCHHEEEECCCCCEEEECCCEEEECCCCEEEECEEEEEECCCCCCCCCCCE
KTGDIGYLDDDLNIVVTGREKEQINRSGEKVTPSEIEEFILQYPTVKDVCVIGVSDEHLG
ECCCCCEECCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHC
ERIKAIIIPKHEDHEISLKNIRKFLTGKNIAHFKIPDEIDVVADFKYTHVGKVNRQKLG
CCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCEEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]