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The map label for this gene is grsT [H]

Identifier: 50085821

GI number: 50085821

Start: 2705698

End: 2706438

Strand: Direct

Name: grsT [H]

Synonym: ACIAD2765

Alternate gene names: 50085821

Gene position: 2705698-2706438 (Clockwise)

Preceding gene: 50085816

Following gene: 50085822

Centisome position: 75.19

GC content: 31.71

Gene sequence:

>741_bases
ATGAACAACAATTCAAAAATTAATTTATTTTGTGTTCCTTCTGCAGGAAGCAGTGCATCAATTTTTAGTGCTTGGGATAA
AACAAGTGCTGAAAATTTAAAAGTTATTGCGCTTGAATACCCTGGTCATGGACGAAAAATTCAGCAGTCCCTGAGTCATG
ATCCTGACTTTTTAGCTCATCAATTTAGCTTAGAAATTTTAGCTTATGGTAATACGCCATATGTACTTTTCGGGCATAGT
GTCGGTGCCTCATTGATATGGAAAATTGAAGAAAAACTCAAACTTTTACATGCTGATCATAACCTGATTCTAAAAATTGT
CAGTGCCCGCCCTTCTCCTCTTTTCCAAAATTTAATTCATAACTATAGTCATCTTACCGATGAGCAAATGATCAAAGAAC
TCAGACTTTATAATAATTTTCCTGATGAAATATTAAACAATGCAAGTGCTTTAAATTTTTTTCTTCCTATTATTAAAAAT
GACTTCATTCTAAGTGACAACATGCTGAAAAAACCTATTTTTAAAAGTTCAGTTCCTCTTATGAGTGTTTATGGAGAAGC
AGATCCGTATATCAAAAATCATAATATTATGGAAAGCTGGAGTACTTATACCAATAAATGGTTAGGAGCTCATCAATTTT
CTGGAGATCACTTCTACTTTTTAGATACAACAATTTTACAAAAAATGCTTCATCTTATTCAGGAAAAAATTTACCTTTTA
ATGAAAACAACACTGGTTTAA

Upstream 100 bases:

>100_bases
AGATTAGATTTGACATCTAGCAGATAAATAGTATTTTAAAGTAATTCTCATTTGCATTTATAACTGCATATTTTAGGCTC
GAAATTTATCAGTCACTCTT

Downstream 100 bases:

>100_bases
TTTTTTAAATTACACATTCATATTATGTTTAATCAAGAATATAAACAGACACCTCTGAATTCAGAACAATATTTAGAACT
AAAATTTTCTAATATTGATT

Product: thioesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MNNNSKINLFCVPSAGSSASIFSAWDKTSAENLKVIALEYPGHGRKIQQSLSHDPDFLAHQFSLEILAYGNTPYVLFGHS
VGASLIWKIEEKLKLLHADHNLILKIVSARPSPLFQNLIHNYSHLTDEQMIKELRLYNNFPDEILNNASALNFFLPIIKN
DFILSDNMLKKPIFKSSVPLMSVYGEADPYIKNHNIMESWSTYTNKWLGAHQFSGDHFYFLDTTILQKMLHLIQEKIYLL
MKTTLV

Sequences:

>Translated_246_residues
MNNNSKINLFCVPSAGSSASIFSAWDKTSAENLKVIALEYPGHGRKIQQSLSHDPDFLAHQFSLEILAYGNTPYVLFGHS
VGASLIWKIEEKLKLLHADHNLILKIVSARPSPLFQNLIHNYSHLTDEQMIKELRLYNNFPDEILNNASALNFFLPIIKN
DFILSDNMLKKPIFKSSVPLMSVYGEADPYIKNHNIMESWSTYTNKWLGAHQFSGDHFYFLDTTILQKMLHLIQEKIYLL
MKTTLV
>Mature_246_residues
MNNNSKINLFCVPSAGSSASIFSAWDKTSAENLKVIALEYPGHGRKIQQSLSHDPDFLAHQFSLEILAYGNTPYVLFGHS
VGASLIWKIEEKLKLLHADHNLILKIVSARPSPLFQNLIHNYSHLTDEQMIKELRLYNNFPDEILNNASALNFFLPIIKN
DFILSDNMLKKPIFKSSVPLMSVYGEADPYIKNHNIMESWSTYTNKWLGAHQFSGDHFYFLDTTILQKMLHLIQEKIYLL
MKTTLV

Specific function: Probable thioesterase involved in the biosynthesis of gramicidin S [H]

COG id: COG3208

COG function: function code Q; Predicted thioesterase involved in non-ribosomal peptide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012223
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00975 Thioesterase [H]

EC number: NA

Molecular weight: Translated: 28171; Mature: 28171

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNSKINLFCVPSAGSSASIFSAWDKTSAENLKVIALEYPGHGRKIQQSLSHDPDFLAH
CCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCCHHHHHH
QFSLEILAYGNTPYVLFGHSVGASLIWKIEEKLKLLHADHNLILKIVSARPSPLFQNLIH
HEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
NYSHLTDEQMIKELRLYNNFPDEILNNASALNFFLPIIKNDFILSDNMLKKPIFKSSVPL
HHHCCCHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCEEECCCHHHCCHHHCCCCC
MSVYGEADPYIKNHNIMESWSTYTNKWLGAHQFSGDHFYFLDTTILQKMLHLIQEKIYLL
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCEEEEECHHHHHHHHHHHHHHHHHH
MKTTLV
HHHHCC
>Mature Secondary Structure
MNNNSKINLFCVPSAGSSASIFSAWDKTSAENLKVIALEYPGHGRKIQQSLSHDPDFLAH
CCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCCHHHHHH
QFSLEILAYGNTPYVLFGHSVGASLIWKIEEKLKLLHADHNLILKIVSARPSPLFQNLIH
HEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
NYSHLTDEQMIKELRLYNNFPDEILNNASALNFFLPIIKNDFILSDNMLKKPIFKSSVPL
HHHCCCHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCEEECCCHHHCCHHHCCCCC
MSVYGEADPYIKNHNIMESWSTYTNKWLGAHQFSGDHFYFLDTTILQKMLHLIQEKIYLL
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCEEEEECHHHHHHHHHHHHHHHHHH
MKTTLV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2477357; 7512553 [H]