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The map label for this gene is nemR [H]

Identifier: 50085799

GI number: 50085799

Start: 2682084

End: 2682713

Strand: Reverse

Name: nemR [H]

Synonym: ACIAD2740

Alternate gene names: 50085799

Gene position: 2682713-2682084 (Counterclockwise)

Preceding gene: 50085800

Following gene: 50085798

Centisome position: 74.55

GC content: 41.11

Gene sequence:

>630_bases
ATGACAACACATCAAGTAAAGAAATCTGAAGCAAAACGACAGCACATCTTGGATTCAGGCTTTCATTTGGTTTTACGTAA
AGGATTTGTTGGTGTCGGTTTGCAAGAAATTCTAAAAACCAGTGGTGTACCAAAGGGTTCCTTTTATCATTATTTTGAAT
CCAAGGAAGCCTTTGGTTGTGAGTTACTGAAGCACTACATCTCTGATTATCAAATACGCTTAAATCAACTGTGGACTACA
GAGACCTCTGCACGTGACAAGCTCATGAACTATTTGCAGTGCTGGGTTAAAGATCCTGCAACCGAGCAGAGTTGGGCAGA
ATCTTGCCTGATTGTCAAAATGGCAGCAGAAGTGGCGGACTTGTCTGAAGATATGCGTTTGATCATGAATGATGGTGTCA
AACGATTGATCGCGCGTATGGCAGATTTAATTCGTATAGGGCAGCAGGAGGGATCTATTCAAACCTCCGTAGTTCCAGAT
GTATTGGCTCAGGTGATTTATCAGATGTATCTGGGCGCAGCGTTATTAAGCAAACTTTATAAACATAAAGCACCTTTATT
CCAAGCTTTGGAAAGTACCAAGATGATGCTGGATGGGTGTAATCACGTTCAACAGGATAAAAATCAATGA

Upstream 100 bases:

>100_bases
GCTGTTCGTCAGGGTATCCTGATCGAATAGGGTGCTGTCTGCTTTACATAGGCAGTCACTTCACTATAATAATAGACGAC
TGGTCTATTTTTAAATTGCA

Downstream 100 bases:

>100_bases
AAATTTTAATGGTTCTCACCTCTCACGATCAGCTTGGTAACACAGGCCATAAGACTGGATTTTGGTTGGAAGAATTTACT
TCGCCTTATTATGTATTTAA

Product: TetR/AcrR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTTHQVKKSEAKRQHILDSGFHLVLRKGFVGVGLQEILKTSGVPKGSFYHYFESKEAFGCELLKHYISDYQIRLNQLWTT
ETSARDKLMNYLQCWVKDPATEQSWAESCLIVKMAAEVADLSEDMRLIMNDGVKRLIARMADLIRIGQQEGSIQTSVVPD
VLAQVIYQMYLGAALLSKLYKHKAPLFQALESTKMMLDGCNHVQQDKNQ

Sequences:

>Translated_209_residues
MTTHQVKKSEAKRQHILDSGFHLVLRKGFVGVGLQEILKTSGVPKGSFYHYFESKEAFGCELLKHYISDYQIRLNQLWTT
ETSARDKLMNYLQCWVKDPATEQSWAESCLIVKMAAEVADLSEDMRLIMNDGVKRLIARMADLIRIGQQEGSIQTSVVPD
VLAQVIYQMYLGAALLSKLYKHKAPLFQALESTKMMLDGCNHVQQDKNQ
>Mature_208_residues
TTHQVKKSEAKRQHILDSGFHLVLRKGFVGVGLQEILKTSGVPKGSFYHYFESKEAFGCELLKHYISDYQIRLNQLWTTE
TSARDKLMNYLQCWVKDPATEQSWAESCLIVKMAAEVADLSEDMRLIMNDGVKRLIARMADLIRIGQQEGSIQTSVVPDV
LAQVIYQMYLGAALLSKLYKHKAPLFQALESTKMMLDGCNHVQQDKNQ

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=186, Percent_Identity=40.3225806451613, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 23800; Mature: 23669

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHQVKKSEAKRQHILDSGFHLVLRKGFVGVGLQEILKTSGVPKGSFYHYFESKEAFGC
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHCCCCHHHH
ELLKHYISDYQIRLNQLWTTETSARDKLMNYLQCWVKDPATEQSWAESCLIVKMAAEVAD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LSEDMRLIMNDGVKRLIARMADLIRIGQQEGSIQTSVVPDVLAQVIYQMYLGAALLSKLY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHKAPLFQALESTKMMLDGCNHVQQDKNQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTHQVKKSEAKRQHILDSGFHLVLRKGFVGVGLQEILKTSGVPKGSFYHYFESKEAFGC
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHCCCCHHHH
ELLKHYISDYQIRLNQLWTTETSARDKLMNYLQCWVKDPATEQSWAESCLIVKMAAEVAD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LSEDMRLIMNDGVKRLIARMADLIRIGQQEGSIQTSVVPDVLAQVIYQMYLGAALLSKLY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHKAPLFQALESTKMMLDGCNHVQQDKNQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]