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The map label for this gene is mii [H]

Identifier: 50085765

GI number: 50085765

Start: 2657701

End: 2658882

Strand: Reverse

Name: mii [H]

Synonym: ACIAD2700

Alternate gene names: 50085765

Gene position: 2658882-2657701 (Counterclockwise)

Preceding gene: 50085780

Following gene: 50085758

Centisome position: 73.89

GC content: 44.92

Gene sequence:

>1182_bases
ATGAAATTTCCTCCTCAGAAAAAAATCGCAGCAACCTATATGCGTGGTGGAACCAGTAAAGGGGTATTTTTTAAGCTAGA
TGATTTACCTGAAGATGCACAGGTGGCTGGAGTTGCCAGAGACCAACTGCTACTACGTGTCATTGGAAGTCCCGATCCTT
ATGCCAAGCAAATTGACGGCATGGGCGGCGCAACATCGAGTACCAGTAAAACAGTGATTCTAGCCAAAAGCCAACAAGCT
GAGCATGATGTTGATTATCTTTTTGGGCAGGTTTCGATTGATCAGCCTTTTGTGGACTGGAGTGGTAACTGTGGTAATTT
GACCGCAGCAGTGGGCTCTTTTGCCATTAGTCATGGTTTGGTTGATGCAAGTCGAATTCCTGAAAATGGAATTTGTACAG
TACGAATCTGGCAAAAAAATATTGAAAAAACCATTATTGCGCATGTTCCTATCACCCATGGTCAAGTACAGGAAACAGGT
GATTTTGAACTGGATGGTGTGACTTTTCCAGCAGCAGAAGTACAAATTGAGTTTCTTGATCCTGCTGATGATGGTGACGA
TGGGGGAGCCATGTTCCCAACGGGTCATGTCGTCGATGAGCTTGATGTACCAGAAATTGGCAGCTTTCAAGCAACATTTA
TCAATGCAGGTATTCCTACAATTTTTCTCAATGCAGAAGAGCTTGGCTATGTAGGCACAGAATTACAGGATCATATTAAT
AGTGATCCTGCTGCATTGGCACGTTTTGAAACTATTCGTGCTTATGGTGCAGTGCAAATGGGCTTAATTCAACATATTAG
CGAGGCTGCATCTCGTCAGCATACCCCTAAAATTGCTTTTGTTGCTCCAGCTAAAACCTATCAGGCTTCTAGTGGAAAAT
CAGTAGATGCCGAACAAATTGATCTCTTGGTTCGTGCATTGTCTATGGGCAAATTACACCATGCCATGATGGGAACGGCG
GCAGTGGCAATTGGTACCGCGGCGGCGATTCCTGGCACCTTAGTCAATCTTGCTGCGGGTGGTGGAGAGCGTGAAGCAGT
ACGTTTTGGACATCCATCTGGCACATTACGGGTTGGAGCACAAGCCATGAAAGATGAAAATGGTCAATGGCAAGTCAAAA
AAGCCATCATGAGTCGTAGTGCACGGGTCTTGATGGAAGGTTTTGTGCGAGTTCCTCAATAG

Upstream 100 bases:

>100_bases
TGTCTATTTAGTATTTCTATCCTAACCAGTCACCGCATAATGATGCAAAATCATTTAAAATGTGGTTACTCAATAAAAAT
GTATATGGACAATCGAGACA

Downstream 100 bases:

>100_bases
CCAAGTGAGAGAAACGATCAACTGGCTGCATTGGCAGTTAATTGGCCTTCTGCAATTAACTGCTCAGCCATTTCCTTGGT
TTTTCTCAAGCCTGGCATAC

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKFPPQKKIAATYMRGGTSKGVFFKLDDLPEDAQVAGVARDQLLLRVIGSPDPYAKQIDGMGGATSSTSKTVILAKSQQA
EHDVDYLFGQVSIDQPFVDWSGNCGNLTAAVGSFAISHGLVDASRIPENGICTVRIWQKNIEKTIIAHVPITHGQVQETG
DFELDGVTFPAAEVQIEFLDPADDGDDGGAMFPTGHVVDELDVPEIGSFQATFINAGIPTIFLNAEELGYVGTELQDHIN
SDPAALARFETIRAYGAVQMGLIQHISEAASRQHTPKIAFVAPAKTYQASSGKSVDAEQIDLLVRALSMGKLHHAMMGTA
AVAIGTAAAIPGTLVNLAAGGGEREAVRFGHPSGTLRVGAQAMKDENGQWQVKKAIMSRSARVLMEGFVRVPQ

Sequences:

>Translated_393_residues
MKFPPQKKIAATYMRGGTSKGVFFKLDDLPEDAQVAGVARDQLLLRVIGSPDPYAKQIDGMGGATSSTSKTVILAKSQQA
EHDVDYLFGQVSIDQPFVDWSGNCGNLTAAVGSFAISHGLVDASRIPENGICTVRIWQKNIEKTIIAHVPITHGQVQETG
DFELDGVTFPAAEVQIEFLDPADDGDDGGAMFPTGHVVDELDVPEIGSFQATFINAGIPTIFLNAEELGYVGTELQDHIN
SDPAALARFETIRAYGAVQMGLIQHISEAASRQHTPKIAFVAPAKTYQASSGKSVDAEQIDLLVRALSMGKLHHAMMGTA
AVAIGTAAAIPGTLVNLAAGGGEREAVRFGHPSGTLRVGAQAMKDENGQWQVKKAIMSRSARVLMEGFVRVPQ
>Mature_393_residues
MKFPPQKKIAATYMRGGTSKGVFFKLDDLPEDAQVAGVARDQLLLRVIGSPDPYAKQIDGMGGATSSTSKTVILAKSQQA
EHDVDYLFGQVSIDQPFVDWSGNCGNLTAAVGSFAISHGLVDASRIPENGICTVRIWQKNIEKTIIAHVPITHGQVQETG
DFELDGVTFPAAEVQIEFLDPADDGDDGGAMFPTGHVVDELDVPEIGSFQATFINAGIPTIFLNAEELGYVGTELQDHIN
SDPAALARFETIRAYGAVQMGLIQHISEAASRQHTPKIAFVAPAKTYQASSGKSVDAEQIDLLVRALSMGKLHHAMMGTA
AVAIGTAAAIPGTLVNLAAGGGEREAVRFGHPSGTLRVGAQAMKDENGQWQVKKAIMSRSARVLMEGFVRVPQ

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=387, Percent_Identity=33.8501291989664, Blast_Score=178, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 41808; Mature: 41808

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFPPQKKIAATYMRGGTSKGVFFKLDDLPEDAQVAGVARDQLLLRVIGSPDPYAKQIDG
CCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCC
MGGATSSTSKTVILAKSQQAEHDVDYLFGQVSIDQPFVDWSGNCGNLTAAVGSFAISHGL
CCCCCCCCCCEEEEECCCCCCCCHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCC
VDASRIPENGICTVRIWQKNIEKTIIAHVPITHGQVQETGDFELDGVTFPAAEVQIEFLD
CCHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCEEECCEECCCEEEEEEEEC
PADDGDDGGAMFPTGHVVDELDVPEIGSFQATFINAGIPTIFLNAEELGYVGTELQDHIN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECHHHHCCCCCHHHHHCC
SDPAALARFETIRAYGAVQMGLIQHISEAASRQHTPKIAFVAPAKTYQASSGKSVDAEQI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEECCCCCCCCHHHH
DLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGEREAVRFGHPSGTLRVGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCEEEECCCCCCEEECH
QAMKDENGQWQVKKAIMSRSARVLMEGFVRVPQ
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKFPPQKKIAATYMRGGTSKGVFFKLDDLPEDAQVAGVARDQLLLRVIGSPDPYAKQIDG
CCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCC
MGGATSSTSKTVILAKSQQAEHDVDYLFGQVSIDQPFVDWSGNCGNLTAAVGSFAISHGL
CCCCCCCCCCEEEEECCCCCCCCHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCC
VDASRIPENGICTVRIWQKNIEKTIIAHVPITHGQVQETGDFELDGVTFPAAEVQIEFLD
CCHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCEEECCEECCCEEEEEEEEC
PADDGDDGGAMFPTGHVVDELDVPEIGSFQATFINAGIPTIFLNAEELGYVGTELQDHIN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECHHHHCCCCCHHHHHCC
SDPAALARFETIRAYGAVQMGLIQHISEAASRQHTPKIAFVAPAKTYQASSGKSVDAEQI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEECCCCCCCCHHHH
DLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGEREAVRFGHPSGTLRVGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCEEEECCCCCCEEECH
QAMKDENGQWQVKKAIMSRSARVLMEGFVRVPQ
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA