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The map label for this gene is gdh [H]

Identifier: 50085746

GI number: 50085746

Start: 2634401

End: 2635672

Strand: Reverse

Name: gdh [H]

Synonym: ACIAD2680

Alternate gene names: 50085746

Gene position: 2635672-2634401 (Counterclockwise)

Preceding gene: 50085747

Following gene: 50085745

Centisome position: 73.24

GC content: 44.1

Gene sequence:

>1272_bases
ATGTCACTTTCATATGAAATAGAAAATAATGGTGCATGGCAAACCTATTTGGCGCAGGTAGATCGTGTTGCACCTTATCT
TGAAGCAGATCTCATTCCTTATATTAATACCTTAAAGCGACCAAAAAGAGCGTTGATTGTTGATGTTCCGATTGTGATGG
ATGATGGGGCGATCCAGCATTTTGAGGGTTACCGAGTCCAACATAATTTGTCTCGTGGTCCAGGCAAAGGCGGGATTCGC
TATCATCCAGATGTTGAGCTCAACGAAGTGATGGCGCTCTCGGCATGGATGACGATCAAGACAGCAGTTTTAAATTTACC
TTATGGCGGGGCAAAAGGAGGGATTCGAGTTGATCCTAAAAAGCTATCAGCCCGAGAGCTGGAACGTTTAACCCGTCGTT
TTACCAGTGAAATTAGCCCGATTATTGGCCCACAGAATGATATTCCTGCACCAGATGTAGGAACCAATGCCGATATCATG
GGCTGGATGATGGATACCTATTCAAGTATCAAAGGCCATACGGTTACAGGTGTGGTCACTGGTAAACCTGTCCATTTGGG
AGGTTCCCTCGGGCGAGTACGCGCGACGGGTCGTGGTGTGTTTGTCACTGGACTTGAAGTCGCGAAAAAGATTAATTTGT
CTATCGAAAATAGCCGTGTGGCTGTACAAGGCTTTGGTAATGTAGGTAGTGAAGCTGCTTATCTGTTTAATAAAGCGAAT
GCCAAAGTGGTCTGTGCACAGGATCATACAGGGACAATCTTTAATGATGACGGATTGGATGTTAAAAGTTTACAAGAGCA
TGTGGCACAAACAGGTGGTGTTGCAGGCTTTCCAGATTCAAGCAAAATAGAGGATGCTGAATTCTGGAATGTCGAGATGG
ATATTCTGGTTCCTGCTGCACTTGAAGGTCAAATCACAGCCGAACGTGCACAAACGCTGAAAGCAAAACTAGTGTTAGAA
GGTGCAAATGGTCCAACGTATCCAGATGCAGATGATGTGTTGGGGCAGCGAAATATCATTGTAGTTCCTGATGTATTATG
TAATGCAGGTGGTGTCACCGTCAGTTATTTTGAATGGGTACAGGATATGTCAAGTTATTTCTGGACTGAGGACGAAATTA
ATGCACGTTTGGATAAATTAATGATTCAGGCTGTGGATGATGTCTGGAAAAAAGCCCAAGAAAAATCTTGTACGATGCGT
ACAGCCGCTTATATTCTGGCCTGTGAGCGCATTTTAAAAGCACGTAAGGAACGTGGTATCTTTCCTGGTTAA

Upstream 100 bases:

>100_bases
ATTATATTAAAAAATAATAAATCAATCGTTTGTGTCTATGATCGAAAAATTAAGTTTTTTTAAACTTATTGAATGAATAA
ATAATATATAGGAAATAATT

Downstream 100 bases:

>100_bases
TAGCTTTAGCCAGAGCAGTTTATCATCAGAGACAAAAATAGAGTGTGATGACAGTGTATCAAGAATAGTACTAAATACGC
TGTCATTTCTATCGGTTCAA

Product: glutamate dehydrogenase (NAD(P)+) oxidoreductase protein

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSLSYEIENNGAWQTYLAQVDRVAPYLEADLIPYINTLKRPKRALIVDVPIVMDDGAIQHFEGYRVQHNLSRGPGKGGIR
YHPDVELNEVMALSAWMTIKTAVLNLPYGGAKGGIRVDPKKLSARELERLTRRFTSEISPIIGPQNDIPAPDVGTNADIM
GWMMDTYSSIKGHTVTGVVTGKPVHLGGSLGRVRATGRGVFVTGLEVAKKINLSIENSRVAVQGFGNVGSEAAYLFNKAN
AKVVCAQDHTGTIFNDDGLDVKSLQEHVAQTGGVAGFPDSSKIEDAEFWNVEMDILVPAALEGQITAERAQTLKAKLVLE
GANGPTYPDADDVLGQRNIIVVPDVLCNAGGVTVSYFEWVQDMSSYFWTEDEINARLDKLMIQAVDDVWKKAQEKSCTMR
TAAYILACERILKARKERGIFPG

Sequences:

>Translated_423_residues
MSLSYEIENNGAWQTYLAQVDRVAPYLEADLIPYINTLKRPKRALIVDVPIVMDDGAIQHFEGYRVQHNLSRGPGKGGIR
YHPDVELNEVMALSAWMTIKTAVLNLPYGGAKGGIRVDPKKLSARELERLTRRFTSEISPIIGPQNDIPAPDVGTNADIM
GWMMDTYSSIKGHTVTGVVTGKPVHLGGSLGRVRATGRGVFVTGLEVAKKINLSIENSRVAVQGFGNVGSEAAYLFNKAN
AKVVCAQDHTGTIFNDDGLDVKSLQEHVAQTGGVAGFPDSSKIEDAEFWNVEMDILVPAALEGQITAERAQTLKAKLVLE
GANGPTYPDADDVLGQRNIIVVPDVLCNAGGVTVSYFEWVQDMSSYFWTEDEINARLDKLMIQAVDDVWKKAQEKSCTMR
TAAYILACERILKARKERGIFPG
>Mature_422_residues
SLSYEIENNGAWQTYLAQVDRVAPYLEADLIPYINTLKRPKRALIVDVPIVMDDGAIQHFEGYRVQHNLSRGPGKGGIRY
HPDVELNEVMALSAWMTIKTAVLNLPYGGAKGGIRVDPKKLSARELERLTRRFTSEISPIIGPQNDIPAPDVGTNADIMG
WMMDTYSSIKGHTVTGVVTGKPVHLGGSLGRVRATGRGVFVTGLEVAKKINLSIENSRVAVQGFGNVGSEAAYLFNKANA
KVVCAQDHTGTIFNDDGLDVKSLQEHVAQTGGVAGFPDSSKIEDAEFWNVEMDILVPAALEGQITAERAQTLKAKLVLEG
ANGPTYPDADDVLGQRNIIVVPDVLCNAGGVTVSYFEWVQDMSSYFWTEDEINARLDKLMIQAVDDVWKKAQEKSCTMRT
AAYILACERILKARKERGIFPG

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=344, Percent_Identity=44.1860465116279, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI4885281, Length=344, Percent_Identity=43.8953488372093, Blast_Score=285, Evalue=4e-77,
Organism=Escherichia coli, GI1788059, Length=388, Percent_Identity=31.701030927835, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17544676, Length=381, Percent_Identity=38.5826771653543, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6319256, Length=393, Percent_Identity=32.824427480916, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6324951, Length=386, Percent_Identity=31.0880829015544, Blast_Score=145, Evalue=9e-36,
Organism=Drosophila melanogaster, GI45549226, Length=364, Percent_Identity=41.7582417582418, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI45553475, Length=353, Percent_Identity=42.7762039660057, Blast_Score=274, Evalue=7e-74,
Organism=Drosophila melanogaster, GI24649283, Length=350, Percent_Identity=42.2857142857143, Blast_Score=269, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24649460, Length=314, Percent_Identity=41.4012738853503, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46252; Mature: 46121

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSYEIENNGAWQTYLAQVDRVAPYLEADLIPYINTLKRPKRALIVDVPIVMDDGAIQH
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHH
FEGYRVQHNLSRGPGKGGIRYHPDVELNEVMALSAWMTIKTAVLNLPYGGAKGGIRVDPK
CCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHEEHHHEECCCCCCCCCEEECHH
KLSARELERLTRRFTSEISPIIGPQNDIPAPDVGTNADIMGWMMDTYSSIKGHTVTGVVT
HCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEE
GKPVHLGGSLGRVRATGRGVFVTGLEVAKKINLSIENSRVAVQGFGNVGSEAAYLFNKAN
CCEEEECCCCCEEEECCCEEEEEHHHHHHHCCEEEECCEEEEEECCCCCCCCEEEEECCC
AKVVCAQDHTGTIFNDDGLDVKSLQEHVAQTGGVAGFPDSSKIEDAEFWNVEMDILVPAA
CEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCC
LEGQITAERAQTLKAKLVLEGANGPTYPDADDVLGQRNIIVVPDVLCNAGGVTVSYFEWV
CCCCEEHHHHHHEEEEEEEECCCCCCCCCHHHHCCCCCEEEECHHHHCCCCCCHHHHHHH
QDMSSYFWTEDEINARLDKLMIQAVDDVWKKAQEKSCTMRTAAYILACERILKARKERGI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FPG
CCC
>Mature Secondary Structure 
SLSYEIENNGAWQTYLAQVDRVAPYLEADLIPYINTLKRPKRALIVDVPIVMDDGAIQH
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHH
FEGYRVQHNLSRGPGKGGIRYHPDVELNEVMALSAWMTIKTAVLNLPYGGAKGGIRVDPK
CCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHEEHHHEECCCCCCCCCEEECHH
KLSARELERLTRRFTSEISPIIGPQNDIPAPDVGTNADIMGWMMDTYSSIKGHTVTGVVT
HCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEE
GKPVHLGGSLGRVRATGRGVFVTGLEVAKKINLSIENSRVAVQGFGNVGSEAAYLFNKAN
CCEEEECCCCCEEEECCCEEEEEHHHHHHHCCEEEECCEEEEEECCCCCCCCEEEEECCC
AKVVCAQDHTGTIFNDDGLDVKSLQEHVAQTGGVAGFPDSSKIEDAEFWNVEMDILVPAA
CEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCC
LEGQITAERAQTLKAKLVLEGANGPTYPDADDVLGQRNIIVVPDVLCNAGGVTVSYFEWV
CCCCEEHHHHHHEEEEEEEECCCCCCCCCHHHHCCCCCEEEECHHHHCCCCCCHHHHHHH
QDMSSYFWTEDEINARLDKLMIQAVDDVWKKAQEKSCTMRTAAYILACERILKARKERGI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FPG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]