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The map label for this gene is ntaB [H]

Identifier: 50085741

GI number: 50085741

Start: 2628937

End: 2629482

Strand: Reverse

Name: ntaB [H]

Synonym: ACIAD2674

Alternate gene names: 50085741

Gene position: 2629482-2628937 (Counterclockwise)

Preceding gene: 50085742

Following gene: 50085740

Centisome position: 73.07

GC content: 42.86

Gene sequence:

>546_bases
GTGAATGTCATGAATATTAATACATCACATGAGCTCGGTTTAAAGCCGATTGATACAGAAAATCCGAGAGAAATCCGAAA
TTTACTTGGACAGTTTGCAACTGGCGTAACCGTGATTACCACGCGTGGTCGTGATGGACGAAAAATCGGAATGACCGCCA
ATTCATTTTCATCATTATCACTTGATCCACCCTTAATTTTGTGGAGTTTGTCAAAAACTGCACCGAGTCTGCCAGACTTT
ACTGAGGCGGAATATTTCGCGATTCACATGCTGGCTCAAGAGCATCATTCACTTTCTGGACATTTTGCACGGGGTTCAGA
AGACAAATTCGCCAGTATTGCACATCGTGAATGTGAACGTGGCCTACCTTTGCTTGAAGATGTACTTGCGACATTGGTGT
GTAAAAACATTAACCAATATGAAGGCGGTGATCACCTGATTTTTATCGGTCAGATTGAGCATTATCAACAACGCATCGGT
GAGCCATTGGTTTTTCATGCGGGTAAATATCGTATTGCAGCAGAGCATCCAGAGCTCAGTGCATAA

Upstream 100 bases:

>100_bases
CGTAATTTCTCTCCGTGGTGGTTTGATGCAGAGCAGGCACAGCATTTTATCGAATCGAAAAGTTGTCAGAAAGTGGCTTA
ACACACAAGGAAGGTAAAAG

Downstream 100 bases:

>100_bases
CTACAATTATCACGATCACTTATGGTCGTGATTTTTTTCGGGGATCGAAGATGAAGAACAATGTGATTAAAAGAGGTTTT
CTTGCTGGAACAGTGGTCAT

Product: electron transfer flavoprotein

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MNVMNINTSHELGLKPIDTENPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPSLPDF
TEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNINQYEGGDHLIFIGQIEHYQQRIG
EPLVFHAGKYRIAAEHPELSA

Sequences:

>Translated_181_residues
MNVMNINTSHELGLKPIDTENPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPSLPDF
TEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNINQYEGGDHLIFIGQIEHYQQRIG
EPLVFHAGKYRIAAEHPELSA
>Mature_181_residues
MNVMNINTSHELGLKPIDTENPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPSLPDF
TEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNINQYEGGDHLIFIGQIEHYQQRIG
EPLVFHAGKYRIAAEHPELSA

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 20145; Mature: 20145

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVMNINTSHELGLKPIDTENPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLS
CCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCEECCCCCCCCCCC
LDPPLILWSLSKTAPSLPDFTEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECER
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
GLPLLEDVLATLVCKNINQYEGGDHLIFIGQIEHYQQRIGEPLVFHAGKYRIAAEHPELS
CCCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHCCCEEEECCCEEEEECCCCCC
A
C
>Mature Secondary Structure
MNVMNINTSHELGLKPIDTENPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLS
CCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCEECCCCCCCCCCC
LDPPLILWSLSKTAPSLPDFTEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECER
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
GLPLLEDVLATLVCKNINQYEGGDHLIFIGQIEHYQQRIGEPLVFHAGKYRIAAEHPELS
CCCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHCCCEEEECCCEEEEECCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]