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The map label for this gene is purN [H]

Identifier: 50085705

GI number: 50085705

Start: 2588509

End: 2589138

Strand: Direct

Name: purN [H]

Synonym: ACIAD2635

Alternate gene names: 50085705

Gene position: 2588509-2589138 (Clockwise)

Preceding gene: 50085704

Following gene: 50085708

Centisome position: 71.93

GC content: 40.32

Gene sequence:

>630_bases
ATGATAAAAATTGCGGTACTTGTTTCTGGAAATGGTAGTAATTTACAAGCCCTGATAGATGCCAATCTGTCAGGCAGCAT
AGTCGGTGTTATTTCCAACAAACCTGATGCGTATGCTTTAAAACGTGCGGAACAAGCCAACATCCAAACCAAAGTGATTG
AGCATAAAACGTATCCTACTCGTGAGCTGTTTGATGATGCGATGCATCAGCAACTCATTGAATGGAATATTGATTTGGTT
GTGCTTGCTGGTTTTATGCGAATTTTAAGTGAAAAGTTTGTAAGGCAGTGGCAAGGGAAAATGATTAATATCCATCCTTC
TCTGCTCCCACTTTACAAAGGTATGCATACTCATCAGCGCGTTTTAAATACTGGTGATGTTTATCATGGCTGTACAGTTC
ACTACGTGACAGCAGAGTTGGATGCTGGTCCTTCGCTATTACAAGGTGTGCTAAAAGTTGAGCAACACGATACAGTGGCT
ACTTTAGCAAATCGGATCCACGAACTGGAACATGTCATTTATCCTCAAGTGGTTGAGTGGATCTGTACCAATATAATCCA
GTATCAGTCAGATCAGGTATTATTAAAAGGTAAGCCTTTACTCAAGCCGTTACAGTTCTGTCAGATTTAA

Upstream 100 bases:

>100_bases
TGCTCAATGCTCAAGGTGAAAAATCTTGGAAAATTGGTCATATTCAAGATAATGCTGAATCTATTGAAGGTGCAGACGAA
AAAATTCGTGTGATCTTTGC

Downstream 100 bases:

>100_bases
AAATCAACCCAATATGTCATAAAAAAGCGCGCCATCCAGGCGCGCTTTTTTATGTCTATTTATGCTCGAATCAACTTCTG
AATCTGATTTACAGTTTCCT

Product: phosphoribosylglycinamide formyltransferase 1

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MIKIAVLVSGNGSNLQALIDANLSGSIVGVISNKPDAYALKRAEQANIQTKVIEHKTYPTRELFDDAMHQQLIEWNIDLV
VLAGFMRILSEKFVRQWQGKMINIHPSLLPLYKGMHTHQRVLNTGDVYHGCTVHYVTAELDAGPSLLQGVLKVEQHDTVA
TLANRIHELEHVIYPQVVEWICTNIIQYQSDQVLLKGKPLLKPLQFCQI

Sequences:

>Translated_209_residues
MIKIAVLVSGNGSNLQALIDANLSGSIVGVISNKPDAYALKRAEQANIQTKVIEHKTYPTRELFDDAMHQQLIEWNIDLV
VLAGFMRILSEKFVRQWQGKMINIHPSLLPLYKGMHTHQRVLNTGDVYHGCTVHYVTAELDAGPSLLQGVLKVEQHDTVA
TLANRIHELEHVIYPQVVEWICTNIIQYQSDQVLLKGKPLLKPLQFCQI
>Mature_209_residues
MIKIAVLVSGNGSNLQALIDANLSGSIVGVISNKPDAYALKRAEQANIQTKVIEHKTYPTRELFDDAMHQQLIEWNIDLV
VLAGFMRILSEKFVRQWQGKMINIHPSLLPLYKGMHTHQRVLNTGDVYHGCTVHYVTAELDAGPSLLQGVLKVEQHDTVA
TLANRIHELEHVIYPQVVEWICTNIIQYQSDQVLLKGKPLLKPLQFCQI

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=189, Percent_Identity=50.2645502645503, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI209869995, Length=189, Percent_Identity=50.2645502645503, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI209869993, Length=189, Percent_Identity=50.2645502645503, Blast_Score=189, Evalue=2e-48,
Organism=Escherichia coli, GI1788846, Length=203, Percent_Identity=43.8423645320197, Blast_Score=181, Evalue=3e-47,
Organism=Escherichia coli, GI1787483, Length=157, Percent_Identity=33.1210191082803, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=192, Percent_Identity=38.0208333333333, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320616, Length=200, Percent_Identity=33.5, Blast_Score=95, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24582400, Length=185, Percent_Identity=43.2432432432432, Blast_Score=168, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23568; Mature: 23568

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIAVLVSGNGSNLQALIDANLSGSIVGVISNKPDAYALKRAEQANIQTKVIEHKTYPT
CEEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCHHHEECCCCCCC
RELFDDAMHQQLIEWNIDLVVLAGFMRILSEKFVRQWQGKMINIHPSLLPLYKGMHTHQR
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCHHHHHH
VLNTGDVYHGCTVHYVTAELDAGPSLLQGVLKVEQHDTVATLANRIHELEHVIYPQVVEW
HHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ICTNIIQYQSDQVLLKGKPLLKPLQFCQI
HHHHHHHCCCCEEEECCCCCHHHHHHHCC
>Mature Secondary Structure
MIKIAVLVSGNGSNLQALIDANLSGSIVGVISNKPDAYALKRAEQANIQTKVIEHKTYPT
CEEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCHHHEECCCCCCC
RELFDDAMHQQLIEWNIDLVVLAGFMRILSEKFVRQWQGKMINIHPSLLPLYKGMHTHQR
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCHHHHHH
VLNTGDVYHGCTVHYVTAELDAGPSLLQGVLKVEQHDTVATLANRIHELEHVIYPQVVEW
HHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ICTNIIQYQSDQVLLKGKPLLKPLQFCQI
HHHHHHHCCCCEEEECCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]