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The map label for this gene is lepB [H]

Identifier: 50085657

GI number: 50085657

Start: 2540045

End: 2540872

Strand: Reverse

Name: lepB [H]

Synonym: ACIAD2583

Alternate gene names: 50085657

Gene position: 2540872-2540045 (Counterclockwise)

Preceding gene: 50085658

Following gene: 50085656

Centisome position: 70.61

GC content: 39.73

Gene sequence:

>828_bases
ATGGATTTTGATTTTAATTTGATTCTCGTTCCAGTCACGCTACTGTTTTTTATTGTGTGGCTTTTGGATAAGCTGGTTTT
AAAACAACGCGCCACAAAAGGACGAGACAACGAAAACTTTATCATTACTTGGGCGTATGACTTCTGGCCTGTTTTGGCTG
TGGTATTGGTACTTCGTTCGTTTATTATCGAGCCGTTTAATATTCCTTCGGATTCGATGGTGCCAACACTAGAAACTGGT
GATTTTATTCTGGTTAACAAATACGATTATGGTGTGCGTTTGCCGATCATCAACAAAAAAGTGATTGATGTGGGTGAGCC
AGAACGTGGTGATGTAATTGTTTTCCGTTATCCGCCACAACCGACCATTAGTTACATCAAGCGTGTTATTGGTTTACCTG
GAGATCATATTGTGTATGATCACGGTCAATTAAGTATTAATGGACAAAAAGTTGCTAAAGTGCCGACGCAGTTTAGCCGT
CCTAAAGATATTCAAGATACTCCAACCTCGATTTATCACAAAGAAACCATTGGCAAGCATACTTTTACCATGCGTGAACT
AGAAGGCGTCAATATTGCGCGTCAGGCACCTTTTATTAATTATATTGACAATGGTAAGTATTCAACTGAAGATGGTTTGT
ATTGGGAAGTGACTGTACCAAAAGGACATTATTTTGCGATGGGTGATAATCGCGATCAGAGTGCGGACAGTCGTTTCTGG
GGATTTGTACCTGAAGAAAATCTGACTGGTCGTGCATTCTATATCTGGATGCATAAAGAGCCAGGCTTTAAGATTCCATC
TTTTAACCGAAATGGTACAATTGACTAA

Upstream 100 bases:

>100_bases
AGGTAAGAAGCGTATGAAACAGGTGGGAAGTGTTGAAATCCCGCAAGAAGCCTTCTTAGCTGTACTTAAAGTAGAGAGAT
AACCCGTTTAGGATTCGCCC

Downstream 100 bases:

>100_bases
AACTAAAAATCAGGAAAATAACAAATGCGGAAGTCTCAGCGGGGTGCTTCGTATTTAGGAATTTTTTTTGGGGTTATTGT
ATTTGCTTTGGTTGTAAAAG

Product: leader peptidase (signal peptidase I), serine protease

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MDFDFNLILVPVTLLFFIVWLLDKLVLKQRATKGRDNENFIITWAYDFWPVLAVVLVLRSFIIEPFNIPSDSMVPTLETG
DFILVNKYDYGVRLPIINKKVIDVGEPERGDVIVFRYPPQPTISYIKRVIGLPGDHIVYDHGQLSINGQKVAKVPTQFSR
PKDIQDTPTSIYHKETIGKHTFTMRELEGVNIARQAPFINYIDNGKYSTEDGLYWEVTVPKGHYFAMGDNRDQSADSRFW
GFVPEENLTGRAFYIWMHKEPGFKIPSFNRNGTID

Sequences:

>Translated_275_residues
MDFDFNLILVPVTLLFFIVWLLDKLVLKQRATKGRDNENFIITWAYDFWPVLAVVLVLRSFIIEPFNIPSDSMVPTLETG
DFILVNKYDYGVRLPIINKKVIDVGEPERGDVIVFRYPPQPTISYIKRVIGLPGDHIVYDHGQLSINGQKVAKVPTQFSR
PKDIQDTPTSIYHKETIGKHTFTMRELEGVNIARQAPFINYIDNGKYSTEDGLYWEVTVPKGHYFAMGDNRDQSADSRFW
GFVPEENLTGRAFYIWMHKEPGFKIPSFNRNGTID
>Mature_275_residues
MDFDFNLILVPVTLLFFIVWLLDKLVLKQRATKGRDNENFIITWAYDFWPVLAVVLVLRSFIIEPFNIPSDSMVPTLETG
DFILVNKYDYGVRLPIINKKVIDVGEPERGDVIVFRYPPQPTISYIKRVIGLPGDHIVYDHGQLSINGQKVAKVPTQFSR
PKDIQDTPTSIYHKETIGKHTFTMRELEGVNIARQAPFINYIDNGKYSTEDGLYWEVTVPKGHYFAMGDNRDQSADSRFW
GFVPEENLTGRAFYIWMHKEPGFKIPSFNRNGTID

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=308, Percent_Identity=38.6363636363636, Blast_Score=178, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 31726; Mature: 31726

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00501 SPASE_I_1 ; PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFDFNLILVPVTLLFFIVWLLDKLVLKQRATKGRDNENFIITWAYDFWPVLAVVLVLRS
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHH
FIIEPFNIPSDSMVPTLETGDFILVNKYDYGVRLPIINKKVIDVGEPERGDVIVFRYPPQ
HHCCCCCCCCCCCCCEECCCCEEEEEECCCCEEEEEECCEEEECCCCCCCCEEEEEECCC
PTISYIKRVIGLPGDHIVYDHGQLSINGQKVAKVPTQFSRPKDIQDTPTSIYHKETIGKH
CHHHHHHHHHCCCCCEEEEECCEEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHCCCC
TFTMRELEGVNIARQAPFINYIDNGKYSTEDGLYWEVTVPKGHYFAMGDNRDQSADSRFW
EEEHHHHCCCCEECCCCEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCEE
GFVPEENLTGRAFYIWMHKEPGFKIPSFNRNGTID
CCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDFDFNLILVPVTLLFFIVWLLDKLVLKQRATKGRDNENFIITWAYDFWPVLAVVLVLRS
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHH
FIIEPFNIPSDSMVPTLETGDFILVNKYDYGVRLPIINKKVIDVGEPERGDVIVFRYPPQ
HHCCCCCCCCCCCCCEECCCCEEEEEECCCCEEEEEECCEEEECCCCCCCCEEEEEECCC
PTISYIKRVIGLPGDHIVYDHGQLSINGQKVAKVPTQFSRPKDIQDTPTSIYHKETIGKH
CHHHHHHHHHCCCCCEEEEECCEEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHCCCC
TFTMRELEGVNIARQAPFINYIDNGKYSTEDGLYWEVTVPKGHYFAMGDNRDQSADSRFW
EEEHHHHCCCCEECCCCEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCEE
GFVPEENLTGRAFYIWMHKEPGFKIPSFNRNGTID
CCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]