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The map label for this gene is pleD [H]

Identifier: 50085580

GI number: 50085580

Start: 2453622

End: 2454584

Strand: Reverse

Name: pleD [H]

Synonym: ACIAD2500

Alternate gene names: 50085580

Gene position: 2454584-2453622 (Counterclockwise)

Preceding gene: 50085581

Following gene: 50085558

Centisome position: 68.21

GC content: 41.02

Gene sequence:

>963_bases
ATGAGTCAAGACGCGCATCATACTCACTTACCTATATCACATGATCAGGCTGCTATCTTGATTGTGGATGATGTTCCTGA
AAACTTGGGATTACTCCATGAAAGTCTGGATCAGGCAGGTTATCGGGTACTGGTGACTACTGATGGATTAAGTGCAATTG
AGATCGCACATCGTTGCTTGCCAGATATGATCTTGCTCGATGGCAATATGCCACATATGGACGGTTTTGAAAGTTGCCTG
CAACTCAAAGCAAGTCCGATCACCCAGCTTATTCCAGTCATCTTTATGACGGGTCTTTCAGAAACTGAACATATTGTTAA
AGGCTTTCAGGTGGGTGGGGTGGATTATGTGACCAAACCGCTGAATATCGAAGAAGTTTTGGCACGGGTAAAAACGCATC
TGGCCCATGCCAAATTGCTACAGCAGCAAAAACAGGTAATTGATGCAACCGAAGTCGCCATTATTGCACTAGATATACAA
GGGGAAATGGCTTGGCAAACCGATAAGGCCACACTATTTTTGAATCAGTATTTACAGGATCAATCGGGCTTTAAGTTGGG
CTTAAAGCAATGGTTTGCAGACATTAAAAAAGACAAAGACAATAAAAAACGTATGAGCTGTACTTACTCTACCTCGACAC
AGCAACTTCAGTTAATTCTTTTGACCCCTTGGCAAGATGATTTAAGCACGACCAAAAATTATCTGGTGCAAATCAAAATT
TCCAGTACAGCATTGAGTATAGAAGATATTTTAAAATACTGCCCGCAATTGACTCAACGTGAAGCCGAAGTATCGCATTG
GCTTGCACTGGGTAAAACCAATAAAGACATTGCCGAGATTTTGGAATTAAGCCCAAGAACGGTGAATAAACATTTAGAAC
ATATTTTTGAAAAGCTTTGTGTCGAAAACCGTACTGCTGCGGTGGCATATATGAATAGTTTAGTACAAGATCAGAGTCTG
TAA

Upstream 100 bases:

>100_bases
AACATGATTTTCCAGAATATGCAGAACTATGGGGACAATTGAGCCAATCTGTGCAAAAATTCGATCTGAAACATGCCAGA
CAATTGCTTCAGGATGCTCC

Downstream 100 bases:

>100_bases
AAAAATTTGAAGTAAAGTTAGTTTTGAATCAACTTTTGATAGTTTGGACAGCTAGATCTTATCGATGTTGCTTTTAATAT
TTCTAAATCATCTATGCGAT

Product: two-component response regulator protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSQDAHHTHLPISHDQAAILIVDDVPENLGLLHESLDQAGYRVLVTTDGLSAIEIAHRCLPDMILLDGNMPHMDGFESCL
QLKASPITQLIPVIFMTGLSETEHIVKGFQVGGVDYVTKPLNIEEVLARVKTHLAHAKLLQQQKQVIDATEVAIIALDIQ
GEMAWQTDKATLFLNQYLQDQSGFKLGLKQWFADIKKDKDNKKRMSCTYSTSTQQLQLILLTPWQDDLSTTKNYLVQIKI
SSTALSIEDILKYCPQLTQREAEVSHWLALGKTNKDIAEILELSPRTVNKHLEHIFEKLCVENRTAAVAYMNSLVQDQSL

Sequences:

>Translated_320_residues
MSQDAHHTHLPISHDQAAILIVDDVPENLGLLHESLDQAGYRVLVTTDGLSAIEIAHRCLPDMILLDGNMPHMDGFESCL
QLKASPITQLIPVIFMTGLSETEHIVKGFQVGGVDYVTKPLNIEEVLARVKTHLAHAKLLQQQKQVIDATEVAIIALDIQ
GEMAWQTDKATLFLNQYLQDQSGFKLGLKQWFADIKKDKDNKKRMSCTYSTSTQQLQLILLTPWQDDLSTTKNYLVQIKI
SSTALSIEDILKYCPQLTQREAEVSHWLALGKTNKDIAEILELSPRTVNKHLEHIFEKLCVENRTAAVAYMNSLVQDQSL
>Mature_319_residues
SQDAHHTHLPISHDQAAILIVDDVPENLGLLHESLDQAGYRVLVTTDGLSAIEIAHRCLPDMILLDGNMPHMDGFESCLQ
LKASPITQLIPVIFMTGLSETEHIVKGFQVGGVDYVTKPLNIEEVLARVKTHLAHAKLLQQQKQVIDATEVAIIALDIQG
EMAWQTDKATLFLNQYLQDQSGFKLGLKQWFADIKKDKDNKKRMSCTYSTSTQQLQLILLTPWQDDLSTTKNYLVQIKIS
STALSIEDILKYCPQLTQREAEVSHWLALGKTNKDIAEILELSPRTVNKHLEHIFEKLCVENRTAAVAYMNSLVQDQSL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1790860, Length=112, Percent_Identity=38.3928571428571, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790437, Length=145, Percent_Identity=29.6551724137931, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788394, Length=148, Percent_Identity=34.4594594594595, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1786784, Length=132, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786599, Length=152, Percent_Identity=30.2631578947368, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787229, Length=124, Percent_Identity=35.4838709677419, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790299, Length=128, Percent_Identity=32.03125, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=31.4814814814815, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=31.0344827586207, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35961; Mature: 35830

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDAHHTHLPISHDQAAILIVDDVPENLGLLHESLDQAGYRVLVTTDGLSAIEIAHRCL
CCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHC
PDMILLDGNMPHMDGFESCLQLKASPITQLIPVIFMTGLSETEHIVKGFQVGGVDYVTKP
CCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCC
LNIEEVLARVKTHLAHAKLLQQQKQVIDATEVAIIALDIQGEMAWQTDKATLFLNQYLQD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCHHCEEEEEEEECCCEEECCCCHHHHHHHHHCC
QSGFKLGLKQWFADIKKDKDNKKRMSCTYSTSTQQLQLILLTPWQDDLSTTKNYLVQIKI
CCCCHHHHHHHHHHHHCCCCCCHHEEEEECCCCCEEEEEEECCCCHHHHHHCEEEEEEEE
SSTALSIEDILKYCPQLTQREAEVSHWLALGKTNKDIAEILELSPRTVNKHLEHIFEKLC
CCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
VENRTAAVAYMNSLVQDQSL
HCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQDAHHTHLPISHDQAAILIVDDVPENLGLLHESLDQAGYRVLVTTDGLSAIEIAHRCL
CCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHC
PDMILLDGNMPHMDGFESCLQLKASPITQLIPVIFMTGLSETEHIVKGFQVGGVDYVTKP
CCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCC
LNIEEVLARVKTHLAHAKLLQQQKQVIDATEVAIIALDIQGEMAWQTDKATLFLNQYLQD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCHHCEEEEEEEECCCEEECCCCHHHHHHHHHCC
QSGFKLGLKQWFADIKKDKDNKKRMSCTYSTSTQQLQLILLTPWQDDLSTTKNYLVQIKI
CCCCHHHHHHHHHHHHCCCCCCHHEEEEECCCCCEEEEEEECCCCHHHHHHCEEEEEEEE
SSTALSIEDILKYCPQLTQREAEVSHWLALGKTNKDIAEILELSPRTVNKHLEHIFEKLC
CCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
VENRTAAVAYMNSLVQDQSL
HCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA