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The map label for this gene is glnA [H]

Identifier: 50085541

GI number: 50085541

Start: 2420313

End: 2421728

Strand: Reverse

Name: glnA [H]

Synonym: ACIAD2458

Alternate gene names: 50085541

Gene position: 2421728-2420313 (Counterclockwise)

Preceding gene: 50085546

Following gene: 50085539

Centisome position: 67.3

GC content: 44.07

Gene sequence:

>1416_bases
ATGAGCATGGCGAACAAGGTCCTTAAACTCATACAAGAAAGTGGCGCAAAATGGGTCGATTTTCGCTTTACGGATACAAA
AGGGAAAGAGCAGCACGTCACTTACCCAGCTGATTCGATTGATGAAGATACATTTGAAGACGGTAAAATGTTTGACGGTT
CTTCAATTGCGGGCTGGAAAGGTATCGAAGCATCTGACATGATTTTACGTCCAGATGCAGAAACTGGTTTTATCGACCCG
TTCTTTGCTGAGCCAACAGTTGTTGTTACTTGTGACGTAATCGAGCCATCTACTGGCCAAGGTTATGAGCGTGACCCACG
TTCGATTGCTCGTCGTGCAGAAGAGTATTTAAAATCTACAGGTATCGGTGATACTGCATTCTTTGGTCCAGAACCAGAAT
TCTTCGTTTTTGACGAAGTAAAATGGGAAATTGATATGTCTGGCGCGCGTCATACGTTGATCGCCGAAGAAGCTGCATGG
TCTACAGGTAAAGATTACGAGTCTGGTAACTCTGGTCACCGTCCACGTGTTAAAGGCGGTTATTTCCCAGTTCCTCCAGT
TGATTCTTCGCATGATATGCGTGCTGAGATGTGTAACAAAATCGAAGAAATCATGGGGCCTGGTCGTGTAGAAGTACACC
ACCACGAAGTTGCATCTTGCCAGTTAGAAATTGGTGTAAGCTTCAACACTATGGTTCGTAAAGCAGACGAAGTACAACAG
TTTAAATATGCTGTTCACAACGTTGCGCATCAATATGCAAAAACTGCAACTTTTATGCCTAAACCAATGGTAGGTGATAA
CGGTTCTGGTATGCACGTTCACATGTCGATCTCTAAAGACGGCAAGAACCTGTTTGCTGGTGATGAATATGCAGGCCTTT
CAGAAATGGCATTGTACTTCATTGGTGGTGTTATCAAGCACGCGCGTGCCTTGAATGCAATCACAAACCCATCTACAAAC
TCTTATAAGCGTTTGGTTCCACACTTCGAAGCACCAATCATGCTTGCTTACTCAGCGCGTAACCGTTCTGCTTCTATCCG
TATTCCTTACGTTTCTAGCCCGAAAGGCAAGCGTATTGAAGCGCGTTTCCCAGATCCAATGATGAACCCGTACTTAGGTT
TCGCTGCATTGTTGATGGCGGGTATTGATGGTATTCAAAACAAGATCCATCCAGGTGAAGCTGCCGACAAGAACTTGTAC
GATCTTCCTCCTGAAGAAGAAGCAAAAATCCCAACAGTTGCACACAGCCTAGATATGGCGCTTGAAGCTCTTCAAGCTGA
CCATGAATTCTTGTTAAAAGGCGGTGTGTTCACCAAAGAAATGCTTGATGCTTACATCGAACTTAAAACTGAAGAAGTAC
GCCGTTTAAATACGACGACTCACCCAGTTGAATTTGATATGTATTACAGCTTGTAA

Upstream 100 bases:

>100_bases
TCTATTAGGTGTGCTTGGTAAAGTTGGTACGAGATTTGCTTTATAACTGCCAACGACTAACACGCACTTCACAAGTGCAA
TAATACTTCATTGGAGCAAA

Downstream 100 bases:

>100_bases
TTTGCTGTAAATACAAAACCCACGATTTATCGTGGTTTTTTTATGGCTAAAACAAATTTAAAACAAGAAACAATTAAACT
TGAAGAAATTTAAGATAACT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSMANKVLKLIQESGAKWVDFRFTDTKGKEQHVTYPADSIDEDTFEDGKMFDGSSIAGWKGIEASDMILRPDAETGFIDP
FFAEPTVVVTCDVIEPSTGQGYERDPRSIARRAEEYLKSTGIGDTAFFGPEPEFFVFDEVKWEIDMSGARHTLIAEEAAW
STGKDYESGNSGHRPRVKGGYFPVPPVDSSHDMRAEMCNKIEEIMGPGRVEVHHHEVASCQLEIGVSFNTMVRKADEVQQ
FKYAVHNVAHQYAKTATFMPKPMVGDNGSGMHVHMSISKDGKNLFAGDEYAGLSEMALYFIGGVIKHARALNAITNPSTN
SYKRLVPHFEAPIMLAYSARNRSASIRIPYVSSPKGKRIEARFPDPMMNPYLGFAALLMAGIDGIQNKIHPGEAADKNLY
DLPPEEEAKIPTVAHSLDMALEALQADHEFLLKGGVFTKEMLDAYIELKTEEVRRLNTTTHPVEFDMYYSL

Sequences:

>Translated_471_residues
MSMANKVLKLIQESGAKWVDFRFTDTKGKEQHVTYPADSIDEDTFEDGKMFDGSSIAGWKGIEASDMILRPDAETGFIDP
FFAEPTVVVTCDVIEPSTGQGYERDPRSIARRAEEYLKSTGIGDTAFFGPEPEFFVFDEVKWEIDMSGARHTLIAEEAAW
STGKDYESGNSGHRPRVKGGYFPVPPVDSSHDMRAEMCNKIEEIMGPGRVEVHHHEVASCQLEIGVSFNTMVRKADEVQQ
FKYAVHNVAHQYAKTATFMPKPMVGDNGSGMHVHMSISKDGKNLFAGDEYAGLSEMALYFIGGVIKHARALNAITNPSTN
SYKRLVPHFEAPIMLAYSARNRSASIRIPYVSSPKGKRIEARFPDPMMNPYLGFAALLMAGIDGIQNKIHPGEAADKNLY
DLPPEEEAKIPTVAHSLDMALEALQADHEFLLKGGVFTKEMLDAYIELKTEEVRRLNTTTHPVEFDMYYSL
>Mature_470_residues
SMANKVLKLIQESGAKWVDFRFTDTKGKEQHVTYPADSIDEDTFEDGKMFDGSSIAGWKGIEASDMILRPDAETGFIDPF
FAEPTVVVTCDVIEPSTGQGYERDPRSIARRAEEYLKSTGIGDTAFFGPEPEFFVFDEVKWEIDMSGARHTLIAEEAAWS
TGKDYESGNSGHRPRVKGGYFPVPPVDSSHDMRAEMCNKIEEIMGPGRVEVHHHEVASCQLEIGVSFNTMVRKADEVQQF
KYAVHNVAHQYAKTATFMPKPMVGDNGSGMHVHMSISKDGKNLFAGDEYAGLSEMALYFIGGVIKHARALNAITNPSTNS
YKRLVPHFEAPIMLAYSARNRSASIRIPYVSSPKGKRIEARFPDPMMNPYLGFAALLMAGIDGIQNKIHPGEAADKNLYD
LPPEEEAKIPTVAHSLDMALEALQADHEFLLKGGVFTKEMLDAYIELKTEEVRRLNTTTHPVEFDMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=66.7377398720682, Blast_Score=661, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=188, Percent_Identity=29.7872340425532, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52456; Mature: 52325

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMANKVLKLIQESGAKWVDFRFTDTKGKEQHVTYPADSIDEDTFEDGKMFDGSSIAGWK
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCC
GIEASDMILRPDAETGFIDPFFAEPTVVVTCDVIEPSTGQGYERDPRSIARRAEEYLKST
CCCCCCEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHC
GIGDTAFFGPEPEFFVFDEVKWEIDMSGARHTLIAEEAAWSTGKDYESGNSGHRPRVKGG
CCCCCCCCCCCCCEEEEEEEEEEEECCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCC
YFPVPPVDSSHDMRAEMCNKIEEIMGPGRVEVHHHEVASCQLEIGVSFNTMVRKADEVQQ
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEECCCHHHHHHHHHHHHH
FKYAVHNVAHQYAKTATFMPKPMVGDNGSGMHVHMSISKDGKNLFAGDEYAGLSEMALYF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
IGGVIKHARALNAITNPSTNSYKRLVPHFEAPIMLAYSARNRSASIRIPYVSSPKGKRIE
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEE
ARFPDPMMNPYLGFAALLMAGIDGIQNKIHPGEAADKNLYDLPPEEEAKIPTVAHSLDMA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LEALQADHEFLLKGGVFTKEMLDAYIELKTEEVRRLNTTTHPVEFDMYYSL
HHHHHCCCHHHHCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEEEECC
>Mature Secondary Structure 
SMANKVLKLIQESGAKWVDFRFTDTKGKEQHVTYPADSIDEDTFEDGKMFDGSSIAGWK
CHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCC
GIEASDMILRPDAETGFIDPFFAEPTVVVTCDVIEPSTGQGYERDPRSIARRAEEYLKST
CCCCCCEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHC
GIGDTAFFGPEPEFFVFDEVKWEIDMSGARHTLIAEEAAWSTGKDYESGNSGHRPRVKGG
CCCCCCCCCCCCCEEEEEEEEEEEECCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCC
YFPVPPVDSSHDMRAEMCNKIEEIMGPGRVEVHHHEVASCQLEIGVSFNTMVRKADEVQQ
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEECCCHHHHHHHHHHHHH
FKYAVHNVAHQYAKTATFMPKPMVGDNGSGMHVHMSISKDGKNLFAGDEYAGLSEMALYF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
IGGVIKHARALNAITNPSTNSYKRLVPHFEAPIMLAYSARNRSASIRIPYVSSPKGKRIE
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEE
ARFPDPMMNPYLGFAALLMAGIDGIQNKIHPGEAADKNLYDLPPEEEAKIPTVAHSLDMA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LEALQADHEFLLKGGVFTKEMLDAYIELKTEEVRRLNTTTHPVEFDMYYSL
HHHHHCCCHHHHCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]