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The map label for this gene is gloB [H]

Identifier: 50085536

GI number: 50085536

Start: 2416769

End: 2417503

Strand: Direct

Name: gloB [H]

Synonym: ACIAD2452

Alternate gene names: 50085536

Gene position: 2416769-2417503 (Clockwise)

Preceding gene: 50085535

Following gene: 50085537

Centisome position: 67.16

GC content: 37.28

Gene sequence:

>735_bases
ATGATGCGCTATCAGATTGATTATATTGATGTCAAAAATAGTCTTAAAAACTATATCTGGATTTTAAAAGATAGTCATAC
TCAAGAAATTATAGTCGTTGATCCGACTGAATCTTCACTGGTACAACAGTATTGTGAAAAACATCAGGTAAAAATTGCAC
AAATCTGGTTAACGCATTGGCATAAAGATCATATCGGTGGCGTACCTGATCTCATTGATGGTAGTCATATTCCTGTTTAT
GGACCACGAGAAGAACTGTCTAAAATTCCCTTGATTACTCACCCTCTTCAACATGAAAATCACTTTAAGTTTAATCACCT
AAATGTTGAAGTATTAGCAACACCTGGTCATACATTAGGACACGTTGTTTATTTTATTGAAGAAACAGGCAGTTTATTTT
GTGGAGACACATTATTTGCGATGGGATGTGGCCGTGTGTTTGAAGGCAGTTTTGAACAGATGTATCACTCATTAAATCGT
CTGGCCGCATTGCCTACAACCACCAAAGTATATTGCACCCACGAATATACCTTAGCCAATGCAGAATTTGCGGTTACAGT
AGAACCTGACAATCTTGCCTTAAAGGAACGCTTAAAGGAAGTTCAATCGCTTAGATCACGCCACCAAATTACCCTACCAT
CCACAATTGCACTTGAACTTGCAACCAATCCGTTTCTTCGAACTGAAAGTGTAGAAGAGTTTGCAAAAATTAGAACATTA
AAAGACCAGTTCTAA

Upstream 100 bases:

>100_bases
CTTTTGTAGAAATGATTTATTTTTATTCCAATTTGTGCCGAACAAACAATAAATGCTTTAATAAATACCATATGCGATTA
TTAATCAGTTCAGGAAAAAA

Downstream 100 bases:

>100_bases
TTTTTAGTTTTTTAATATTTTGCTTAGTACATATTTATTTACATCTATTTTTATAAAAAAAATATGTACTAAGTTGTTTC
TTAAACTAAAAGATGTTGTT

Product: hydroxyacylglutathione hydrolase (GloB)

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MMRYQIDYIDVKNSLKNYIWILKDSHTQEIIVVDPTESSLVQQYCEKHQVKIAQIWLTHWHKDHIGGVPDLIDGSHIPVY
GPREELSKIPLITHPLQHENHFKFNHLNVEVLATPGHTLGHVVYFIEETGSLFCGDTLFAMGCGRVFEGSFEQMYHSLNR
LAALPTTTKVYCTHEYTLANAEFAVTVEPDNLALKERLKEVQSLRSRHQITLPSTIALELATNPFLRTESVEEFAKIRTL
KDQF

Sequences:

>Translated_244_residues
MMRYQIDYIDVKNSLKNYIWILKDSHTQEIIVVDPTESSLVQQYCEKHQVKIAQIWLTHWHKDHIGGVPDLIDGSHIPVY
GPREELSKIPLITHPLQHENHFKFNHLNVEVLATPGHTLGHVVYFIEETGSLFCGDTLFAMGCGRVFEGSFEQMYHSLNR
LAALPTTTKVYCTHEYTLANAEFAVTVEPDNLALKERLKEVQSLRSRHQITLPSTIALELATNPFLRTESVEEFAKIRTL
KDQF
>Mature_244_residues
MMRYQIDYIDVKNSLKNYIWILKDSHTQEIIVVDPTESSLVQQYCEKHQVKIAQIWLTHWHKDHIGGVPDLIDGSHIPVY
GPREELSKIPLITHPLQHENHFKFNHLNVEVLATPGHTLGHVVYFIEETGSLFCGDTLFAMGCGRVFEGSFEQMYHSLNR
LAALPTTTKVYCTHEYTLANAEFAVTVEPDNLALKERLKEVQSLRSRHQITLPSTIALELATNPFLRTESVEEFAKIRTL
KDQF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=247, Percent_Identity=38.4615384615385, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI94538320, Length=247, Percent_Identity=38.4615384615385, Blast_Score=167, Evalue=5e-42,
Organism=Homo sapiens, GI116642887, Length=218, Percent_Identity=40.8256880733945, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI21703352, Length=218, Percent_Identity=40.8256880733945, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI14150041, Length=226, Percent_Identity=37.1681415929204, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI46361987, Length=193, Percent_Identity=36.7875647668394, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI41327741, Length=200, Percent_Identity=26, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786406, Length=246, Percent_Identity=41.869918699187, Blast_Score=171, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17536925, Length=251, Percent_Identity=34.2629482071713, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320478, Length=234, Percent_Identity=29.9145299145299, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324614, Length=232, Percent_Identity=31.8965517241379, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356335, Length=249, Percent_Identity=38.5542168674699, Blast_Score=171, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24667703, Length=249, Percent_Identity=38.5542168674699, Blast_Score=170, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24667711, Length=249, Percent_Identity=38.5542168674699, Blast_Score=170, Evalue=6e-43,
Organism=Drosophila melanogaster, GI20129357, Length=120, Percent_Identity=36.6666666666667, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221330176, Length=214, Percent_Identity=26.6355140186916, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001018
- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28053; Mature: 28053

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRYQIDYIDVKNSLKNYIWILKDSHTQEIIVVDPTESSLVQQYCEKHQVKIAQIWLTHW
CCEEEEEEEEHHHHHCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCEEEEEEEEHHH
HKDHIGGVPDLIDGSHIPVYGPREELSKIPLITHPLQHENHFKFNHLNVEVLATPGHTLG
CHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCEECCCCCCCCEEEEEEEEEEEECCCCCHH
HVVYFIEETGSLFCGDTLFAMGCGRVFEGSFEQMYHSLNRLAALPTTTKVYCTHEYTLAN
EEEEEEECCCCEEECCHHHHHCCCHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEEEC
AEFAVTVEPDNLALKERLKEVQSLRSRHQITLPSTIALELATNPFLRTESVEEFAKIRTL
CEEEEEECCCCHHHHHHHHHHHHHHHCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHH
KDQF
HCCC
>Mature Secondary Structure
MMRYQIDYIDVKNSLKNYIWILKDSHTQEIIVVDPTESSLVQQYCEKHQVKIAQIWLTHW
CCEEEEEEEEHHHHHCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCEEEEEEEEHHH
HKDHIGGVPDLIDGSHIPVYGPREELSKIPLITHPLQHENHFKFNHLNVEVLATPGHTLG
CHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCEECCCCCCCCEEEEEEEEEEEECCCCCHH
HVVYFIEETGSLFCGDTLFAMGCGRVFEGSFEQMYHSLNRLAALPTTTKVYCTHEYTLAN
EEEEEEECCCCEEECCHHHHHCCCHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEEEC
AEFAVTVEPDNLALKERLKEVQSLRSRHQITLPSTIALELATNPFLRTESVEEFAKIRTL
CEEEEEECCCCHHHHHHHHHHHHHHHCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHH
KDQF
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA