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The map label for this gene is acnB [H]

Identifier: 50085482

GI number: 50085482

Start: 2350951

End: 2353590

Strand: Reverse

Name: acnB [H]

Synonym: ACIAD2395

Alternate gene names: 50085482

Gene position: 2353590-2350951 (Counterclockwise)

Preceding gene: 50085483

Following gene: 50085480

Centisome position: 65.4

GC content: 45.34

Gene sequence:

>2640_bases
GTGCTAGAAGCTTACCGCCAACACGTTGAAGAACGTGCCGCACTCGGAGTCCCACCGAAGCCACTTGATGATGCTCAAAC
TGCTGATTTAGTCGAATTACTTAAAAATCCGCCAGCAGGTGAAGAAGCTTTCCTCGTTGACTTGCTTGAAAATCGTGTTC
CAGCAGGTGTAGACCAAGCGGCATACGTTAAAGCAGCTTTTTTGGCAGCTTTAGCAAAAGGTGAAGCGACCTCTCCACTC
GTTTCTAAAGAACGCGCAGTTTATTTGCTAGGTACCATGCTTGGTGGTTACAACGTTGCACCTCTAGTTGAGCTTTTAGA
AGATGCTGATTTAGGCGCTTTAGCTGCTGATGCATTGAAAAAAACACTTCTTGTATTTGATGCGTTCCATGATGTGGCTG
ATAAAGCAAAAGCGGGTAACACTAACGCAAAAGCAGTTTTACAATCTTGGGCTGATGCTGAGTGGTTTACAAGCCGCAAA
GATGTTCCAGAAGAAATCAAGCTCACTGTTTTTAAAGTGACAGGCGAAACTAACACCGACGACTTGTCTCCAGCGCAAGA
CGCTTGGAGCCGTCCGGATATCCCATTACACGCAAATGCAATGTTGAAAAACGAGCGTGATGGTATTAACCCTGAAAAAC
CAGGTGAAGTTGGTCCACTGAGCCAAATTAAAGAACTCATTGCTAAAGGCAATCAAGTTGCTTATGTCGGTGACGTTGTG
GGTACAGGTTCATCTCGTAAATCTGCAACAAACTCTGTGCTTTGGTTCTTTGGTGACGAAATCGCGCACATTCCGAACAA
AAAAGACGGTGGTTACTGCTTAGGTTCTAAAATCGCACCGATTTTCTTCAACACGATGGAAGACGCTGGTGCGTTACCTG
TAGAGATTGACGTTGCCAATATGAACATGGGCGACGAAATTGTTCTCAAAATTGACCATGCTGCTGCGAAAGTAACGGCG
TTCAAAAATGGCGAACAAATTGCTGAATCTGAACTTAAAACTCCAGTGCTTCTAGACGAAGTACGTGCGGGTGGTCGTAT
TAACCTGATCGTGGGTCGTGGTTTGACCACCAAAGCGCGTGAAGCGTTAGGTCTTGAGCCATCTACATTATTCCGTACCC
CAGTACAACCTGCTGCAACGGGCAAAGGTTTCACTTTAGCTCAGAAGATGGTGGGTCGTGCATGTGGTCTTCCAGAAGGT
CAGGGTGTTATTCCGGGTACTTACTGTGAACCTAAGATGACGACTGTTGGTTCTCAGGATACCACTGGTCCTATGACTCG
TGACGAGTTAAAAGATTTGGCTTGCTTGGGCTTCTCTGCTGACTTGGTCATGCAATCGTTCTGTCACACTGCTGCTTATC
CAAAGCCAGTGGACGTACAAATGCAACACACATTGCCAGACTTCATCATGAACCGTGGTGGTGTATCGCTTCGTCCAGGT
GATGGTATTATCCACTCTTGGTTAAACCGTATGTTGCTTCCAGATACAGTCGGTACTGGCGGTGACTCACATACGCGTTT
CCCAATTGGTATTTCATTCCCTGCGGGTTCTGGCCTTGTTGCGTTCGCGGCAGCGACTGGTGTTATGCCACTCGATATGC
CTGAATCTGTACTTGTGAAGTTCAAAGGTAAAATGCAACCTGGTATCACTTTACGTGACCTTGTACATGCAATTCCTTAC
TACGCAATCAAAGAAGGCGATTTGACTGTAGAGAAAAAAGGTAAGAAAAACATTTTCTCTGGTCGTATCTTGGAAATCGA
CTTAACAGAAATGGAAACAGACCTTACAGTTGAGCAAGCATTTGAATTGTCTGATGCATCTGCTGAGCGTTCGGCTGCGG
GTTGTGCAATCACGCTTTCTGAAGAGAAAGTGGCTGAGTACTTGCGTTCGAACATTACCATGTTGAAGTGGATGATTTCA
CAAGGCTATGGTGATGCACGTACAATGGCGCGCCGTGTTGAAAACATGGAAAAATGGTTGGCTAATCCATCACTTCTTAA
AGCGGATGCGGATGCTGAATACACTAAAGTGTATGAAATTGATCTTGCAGACATCAAAGAACCAATTCTTTGCTGCCCGA
ACGATCCAGATGATGCAAAACTTCTTTCTGATGTTCAAGGCGACAAAATTGATGAAGTGTTCATCGGTTCTTGTATGACC
AACATTGGTCATTTCCGTGCAGCGGGTCAGTTGCTTGAGAAAGTACCAAGTGGTTCATTAACAACTCGTTTATGGTTGGC
ACCACCAACACGTATGGACGAACACCAGTTAATGGAAGAAGGTTTCTACAATACCTATGGTCGCGCTGGTGCGCGTACTG
AAATGCCGGGTTGCTCACTATGTATGGGTAACCAAGCACGTGTTGCGCCAAACACCACGGTCGTATCGACTTCTACACGT
AACTTCCCGAACCGTTTGGGTCAAGGCTCTAACGTATACTTGGCATCTGCTGAGCTTGCATCTGTTGCTGCTGTACTCGG
TAAATTACCGACGCCAGAAGAATATCAACAATATGCAGCACAAATCGACAGTATGTCTGCGGATATCTACAAATATTTGA
ATTTCGACAAAATGGGCGAATATACAAATGCTGCCGATCAAGTAGATACCAAGAAAATTGCTGCAGCTCAATTAACTTAA

Upstream 100 bases:

>100_bases
TTTATCTTGATTTTTAGTAAAATTTTGCTGTCCATATTACTTGTGTAGCTTTGATAAGCCGGAATTCGCAAGTAATTTTT
TAAAAAAGAGGAATAACACT

Downstream 100 bases:

>100_bases
TCGATTAATGATTGAGTTTAAATGAGTAAAAAAGGGCTGAGAAATCAGTCCTTTTTTAATTTTCATGAAACACTTGCTGC
AACAGTTTCTTAAAAGCGAA

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 879; Mature: 879

Protein sequence:

>879_residues
MLEAYRQHVEERAALGVPPKPLDDAQTADLVELLKNPPAGEEAFLVDLLENRVPAGVDQAAYVKAAFLAALAKGEATSPL
VSKERAVYLLGTMLGGYNVAPLVELLEDADLGALAADALKKTLLVFDAFHDVADKAKAGNTNAKAVLQSWADAEWFTSRK
DVPEEIKLTVFKVTGETNTDDLSPAQDAWSRPDIPLHANAMLKNERDGINPEKPGEVGPLSQIKELIAKGNQVAYVGDVV
GTGSSRKSATNSVLWFFGDEIAHIPNKKDGGYCLGSKIAPIFFNTMEDAGALPVEIDVANMNMGDEIVLKIDHAAAKVTA
FKNGEQIAESELKTPVLLDEVRAGGRINLIVGRGLTTKAREALGLEPSTLFRTPVQPAATGKGFTLAQKMVGRACGLPEG
QGVIPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVQMQHTLPDFIMNRGGVSLRPG
DGIIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVKFKGKMQPGITLRDLVHAIPY
YAIKEGDLTVEKKGKKNIFSGRILEIDLTEMETDLTVEQAFELSDASAERSAAGCAITLSEEKVAEYLRSNITMLKWMIS
QGYGDARTMARRVENMEKWLANPSLLKADADAEYTKVYEIDLADIKEPILCCPNDPDDAKLLSDVQGDKIDEVFIGSCMT
NIGHFRAAGQLLEKVPSGSLTTRLWLAPPTRMDEHQLMEEGFYNTYGRAGARTEMPGCSLCMGNQARVAPNTTVVSTSTR
NFPNRLGQGSNVYLASAELASVAAVLGKLPTPEEYQQYAAQIDSMSADIYKYLNFDKMGEYTNAADQVDTKKIAAAQLT

Sequences:

>Translated_879_residues
MLEAYRQHVEERAALGVPPKPLDDAQTADLVELLKNPPAGEEAFLVDLLENRVPAGVDQAAYVKAAFLAALAKGEATSPL
VSKERAVYLLGTMLGGYNVAPLVELLEDADLGALAADALKKTLLVFDAFHDVADKAKAGNTNAKAVLQSWADAEWFTSRK
DVPEEIKLTVFKVTGETNTDDLSPAQDAWSRPDIPLHANAMLKNERDGINPEKPGEVGPLSQIKELIAKGNQVAYVGDVV
GTGSSRKSATNSVLWFFGDEIAHIPNKKDGGYCLGSKIAPIFFNTMEDAGALPVEIDVANMNMGDEIVLKIDHAAAKVTA
FKNGEQIAESELKTPVLLDEVRAGGRINLIVGRGLTTKAREALGLEPSTLFRTPVQPAATGKGFTLAQKMVGRACGLPEG
QGVIPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVQMQHTLPDFIMNRGGVSLRPG
DGIIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVKFKGKMQPGITLRDLVHAIPY
YAIKEGDLTVEKKGKKNIFSGRILEIDLTEMETDLTVEQAFELSDASAERSAAGCAITLSEEKVAEYLRSNITMLKWMIS
QGYGDARTMARRVENMEKWLANPSLLKADADAEYTKVYEIDLADIKEPILCCPNDPDDAKLLSDVQGDKIDEVFIGSCMT
NIGHFRAAGQLLEKVPSGSLTTRLWLAPPTRMDEHQLMEEGFYNTYGRAGARTEMPGCSLCMGNQARVAPNTTVVSTSTR
NFPNRLGQGSNVYLASAELASVAAVLGKLPTPEEYQQYAAQIDSMSADIYKYLNFDKMGEYTNAADQVDTKKIAAAQLT
>Mature_879_residues
MLEAYRQHVEERAALGVPPKPLDDAQTADLVELLKNPPAGEEAFLVDLLENRVPAGVDQAAYVKAAFLAALAKGEATSPL
VSKERAVYLLGTMLGGYNVAPLVELLEDADLGALAADALKKTLLVFDAFHDVADKAKAGNTNAKAVLQSWADAEWFTSRK
DVPEEIKLTVFKVTGETNTDDLSPAQDAWSRPDIPLHANAMLKNERDGINPEKPGEVGPLSQIKELIAKGNQVAYVGDVV
GTGSSRKSATNSVLWFFGDEIAHIPNKKDGGYCLGSKIAPIFFNTMEDAGALPVEIDVANMNMGDEIVLKIDHAAAKVTA
FKNGEQIAESELKTPVLLDEVRAGGRINLIVGRGLTTKAREALGLEPSTLFRTPVQPAATGKGFTLAQKMVGRACGLPEG
QGVIPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVQMQHTLPDFIMNRGGVSLRPG
DGIIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVKFKGKMQPGITLRDLVHAIPY
YAIKEGDLTVEKKGKKNIFSGRILEIDLTEMETDLTVEQAFELSDASAERSAAGCAITLSEEKVAEYLRSNITMLKWMIS
QGYGDARTMARRVENMEKWLANPSLLKADADAEYTKVYEIDLADIKEPILCCPNDPDDAKLLSDVQGDKIDEVFIGSCMT
NIGHFRAAGQLLEKVPSGSLTTRLWLAPPTRMDEHQLMEEGFYNTYGRAGARTEMPGCSLCMGNQARVAPNTTVVSTSTR
NFPNRLGQGSNVYLASAELASVAAVLGKLPTPEEYQQYAAQIDSMSADIYKYLNFDKMGEYTNAADQVDTKKIAAAQLT

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=868, Percent_Identity=74.1935483870968, Blast_Score=1287, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=414, Percent_Identity=25.8454106280193, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 94834; Mature: 94834

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRQHVEERAALGVPPKPLDDAQTADLVELLKNPPAGEEAFLVDLLENRVPAGVDQA
CCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHH
AYVKAAFLAALAKGEATSPLVSKERAVYLLGTMLGGYNVAPLVELLEDADLGALAADALK
HHHHHHHHHHHHCCCCCCCHHHHCCEEEEEEHHHCCCCHHHHHHHHHCCCCHHHHHHHHH
KTLLVFDAFHDVADKAKAGNTNAKAVLQSWADAEWFTSRKDVPEEIKLTVFKVTGETNTD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEEEECCCCCC
DLSPAQDAWSRPDIPLHANAMLKNERDGINPEKPGEVGPLSQIKELIAKGNQVAYVGDVV
CCCCHHHHCCCCCCCEECHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECEE
GTGSSRKSATNSVLWFFGDEIAHIPNKKDGGYCLGSKIAPIFFNTMEDAGALPVEIDVAN
CCCCCCCCCCCCEEEEECCHHHHCCCCCCCCEEECCCCCHHHEECHHCCCCCEEEEEEEC
MNMGDEIVLKIDHAAAKVTAFKNGEQIAESELKTPVLLDEVRAGGRINLIVGRGLTTKAR
CCCCCEEEEEEECCCEEEEEECCCHHHHHHHCCCCHHHHHHCCCCEEEEEEECCCCHHHH
EALGLEPSTLFRTPVQPAATGKGFTLAQKMVGRACGLPEGQGVIPGTYCEPKMTTVGSQD
HHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCC
TTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVQMQHTLPDFIMNRGGVSLRPG
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEHHHCCHHHHHCCCCCEECCC
DGIIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVK
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHE
FKGKMQPGITLRDLVHAIPYYAIKEGDLTVEKKGKKNIFSGRILEIDLTEMETDLTVEQA
ECCCCCCCCCHHHHHHHCCCEEEECCCEEEECCCCCCCCCCEEEEEEHHHCCCCCHHHHH
FELSDASAERSAAGCAITLSEEKVAEYLRSNITMLKWMISQGYGDARTMARRVENMEKWL
HHCCCCCHHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ANPSLLKADADAEYTKVYEIDLADIKEPILCCPNDPDDAKLLSDVQGDKIDEVFIGSCMT
CCCCEEECCCCCCEEEEEEEEHHHCCCCEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHH
NIGHFRAAGQLLEKVPSGSLTTRLWLAPPTRMDEHQLMEEGFYNTYGRAGARTEMPGCSL
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCE
CMGNQARVAPNTTVVSTSTRNFPNRLGQGSNVYLASAELASVAAVLGKLPTPEEYQQYAA
EECCCCEECCCCEEEECCCCCCHHHHCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHH
QIDSMSADIYKYLNFDKMGEYTNAADQVDTKKIAAAQLT
HHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLEAYRQHVEERAALGVPPKPLDDAQTADLVELLKNPPAGEEAFLVDLLENRVPAGVDQA
CCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHH
AYVKAAFLAALAKGEATSPLVSKERAVYLLGTMLGGYNVAPLVELLEDADLGALAADALK
HHHHHHHHHHHHCCCCCCCHHHHCCEEEEEEHHHCCCCHHHHHHHHHCCCCHHHHHHHHH
KTLLVFDAFHDVADKAKAGNTNAKAVLQSWADAEWFTSRKDVPEEIKLTVFKVTGETNTD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEEEECCCCCC
DLSPAQDAWSRPDIPLHANAMLKNERDGINPEKPGEVGPLSQIKELIAKGNQVAYVGDVV
CCCCHHHHCCCCCCCEECHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECEE
GTGSSRKSATNSVLWFFGDEIAHIPNKKDGGYCLGSKIAPIFFNTMEDAGALPVEIDVAN
CCCCCCCCCCCCEEEEECCHHHHCCCCCCCCEEECCCCCHHHEECHHCCCCCEEEEEEEC
MNMGDEIVLKIDHAAAKVTAFKNGEQIAESELKTPVLLDEVRAGGRINLIVGRGLTTKAR
CCCCCEEEEEEECCCEEEEEECCCHHHHHHHCCCCHHHHHHCCCCEEEEEEECCCCHHHH
EALGLEPSTLFRTPVQPAATGKGFTLAQKMVGRACGLPEGQGVIPGTYCEPKMTTVGSQD
HHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCC
TTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVQMQHTLPDFIMNRGGVSLRPG
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEHHHCCHHHHHCCCCCEECCC
DGIIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVK
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHE
FKGKMQPGITLRDLVHAIPYYAIKEGDLTVEKKGKKNIFSGRILEIDLTEMETDLTVEQA
ECCCCCCCCCHHHHHHHCCCEEEECCCEEEECCCCCCCCCCEEEEEEHHHCCCCCHHHHH
FELSDASAERSAAGCAITLSEEKVAEYLRSNITMLKWMISQGYGDARTMARRVENMEKWL
HHCCCCCHHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ANPSLLKADADAEYTKVYEIDLADIKEPILCCPNDPDDAKLLSDVQGDKIDEVFIGSCMT
CCCCEEECCCCCCEEEEEEEEHHHCCCCEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHH
NIGHFRAAGQLLEKVPSGSLTTRLWLAPPTRMDEHQLMEEGFYNTYGRAGARTEMPGCSL
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCE
CMGNQARVAPNTTVVSTSTRNFPNRLGQGSNVYLASAELASVAAVLGKLPTPEEYQQYAA
EECCCCEECCCCEEEECCCCCCHHHHCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHH
QIDSMSADIYKYLNFDKMGEYTNAADQVDTKKIAAAQLT
HHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]