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The map label for this gene is ostA

Identifier: 50085461

GI number: 50085461

Start: 2328576

End: 2331035

Strand: Direct

Name: ostA

Synonym: ACIAD2371

Alternate gene names: 50085461

Gene position: 2328576-2331035 (Clockwise)

Preceding gene: 50085435

Following gene: 50085462

Centisome position: 64.71

GC content: 40.41

Gene sequence:

>2460_bases
ATGAGACAAATGAAGTATCAGTTTAAATTTAATCCTTTAGCAGCTGCTATTTTTACACTCTTGTGTGGTGGCTCCATGCA
ATCAAGCTATGCTGATGCGAATGACAGCGCCTCGTCTGTCGACAATAAAAAACTCAAGGAGAGCATTCAAAAAGCCTATC
CTGGACAAGAGTTTTTTGAACAGTATTATGTGGAAAAGTCATCTCCTGAAGCGCAGGTTCGTGATACAAGATCACTGAGT
TCAGCATTTTGCACAGGCACATGGATTACACCCATTAGCCCAACCACTCAAGCTGTCCCTGCCGATCAGGCAACATCGGT
GGTCACGGCCGATTATGCACATTACAATCCAAATGGTGATTCAGAGCTTGAAGGCAACGTCCTGATCGATCAGCAAGGGC
GTTCTATTCGTGCAAATAAAGTGACCATTGATCGCACACAGACTTATGCAAATGCTGAAGGCAATGTACAGCTCGCACAA
GCTGGATTGTTAGCACAAAGTGATCAGATTAATTACAATCTTAAAACGCAGCAAGGCGATTTAAAGAACAGCTTTTATAT
TTCAGAACAACAACATGCGCACGGACACGCTGAACAAATTCAACGTACTTCACCTACTGAAATTATATTACGCAATGCCA
CTTATACAACCTGTCCGCCAGAGCAGAAACCAACGTGGCGGCTAGAAGCAAAAGAAATCAAGCTCAATCAGGATACAGGC
CGAGGTACGACAAAAAATACCAAGCTGTATGTGAAAGATGTACCTATTCTTGCTGTTCCGTACTTTAACTTTCCAATTGA
TAACCGTAGAACCACAGGTATTCTAAATCCCAATATCGGTTTTTCAAATGATGGTGGCCTTGAGCTTACTGTTCCTGTCT
ATCTCAACCTTGCACCTAACTATGATGCAACACTCACGCCACGCTACATCTCAGATCGAGGTGTAATGCTTCAAAGTGAA
TTTAGATATTTAACCGAAAACTTCGGTCAAGGGAAAATTTGGGGTGGGTATCTACCAGACGATAAAAAATATAACAATGA
AGATCGTAAAGACTTCAATCTTTTACACAAATGGAAGATTAATGATTACTGGTCTACCGATGTTGAATACCACTATGCAT
CAGACAAAGACTATGTTACCGATTTAGATACCAATCCTGACTCAAAAACAGATTTGAACTTGCGTCGTGCCTGGACACTG
AAATATAAAAATCAAATTCCTGGTTTAACTGCACAACTTAAAGTTGAAGACTTTCAGACACTTGATAAAACAGTAAGTGA
TGTTGATAAACCTTATGCGCGTTTACCTCAGTTTTTATTAAATTACGTGACGGGTAATCCATTAGGTCTACAATACGAAT
TTAACAACGATACGGCTTATTTTAAGAAGAACATCGATGATGCTGCAAACTACAGCACTCAACCTAGCGGTACACGCATT
TATAACCAGTTTGCGACGCGTTATAACTTCAGAACACCTTGGGCATTTGCAATTCCAGAGGTTTCTATTCGGAGTATCAA
CACGTTCTACGATCAAAACACAGTCGAAAATCTAGGATTAAACTCAGACAACAAGAGCAAGTCTGTCGTTGTGCCACAAT
TTAGTCTGGACACAGGTTTGATTTTCCAGCGTGATGGTGACTATCTTCAAACCATTACACCACGTGCATTTTATGCCTAT
GCTCCTTATAAAAACCAAACTGGCTATCCAAATTTTGACACTACATCTGCATCAATTAACTATGACCAATTGTTCAGCCC
TTATCGTTTCTATGGTCATGACCGTCTTGAAGACAACAACTTTTTATCGCTTGGCGTAAGTTATAGTTTATTTGATCCGC
AAGGACTGGAGCGACTTCGCGCAGGCGTTGGTCAAAGCTTCTATTTTGCTGATCGACGTGTCACGTTAAACAATACTGAC
GACACTATTGATACTTCGAAAAACTCAGGCCCTATTGTGAGTATTTCGAGTCAACTGACGAATAAGTTCACAGTTGCTGC
AAATTCGGCATGGATGTCGAATGGTGATAATGCCCAACATGATTTCCAGACTTATTACACAGGCGATCATGGCAATCTTT
ATAATCTCGGTTATTTCAATCGCAAGAATATTCCAGACCGCCAGTTAGCATACGATGCTGCTGTTGCATCATTTGTACAA
CCTATCATGAATAACTGGCGTATTATGGGCCATGTTCAATTTGACTTTAGAAACAATGTTGCGCGCGAATATTTACTTGG
CGTTAACTATGAGTCATGTTGTTATGCGATCTCTGTATACGGCCGTTCATATTATAACGATCTGGACGATCCTAAAGATC
CAAATGTAAACGTTAAACGTGCCGTGATGGCGGAAATCACATTTAAAGGACTAGGCGGCTTAAATAACAAGTTGGCTTCT
TTACTGGAAAATCGCGTTTTAGGCTTTAAAGAAATTAATCAATCTTGGACACAACGTTAA

Upstream 100 bases:

>100_bases
TCTAAAACGGGCTGAGATTTTGAAGTTCTAAGTGTAGCCACTTATAAACTTTTTCTCTATGATGCGACAATAATTAATTG
CGTTTTAGCATACTTGGTTA

Downstream 100 bases:

>100_bases
TGAAGACAAAGTATCTTAAACAGTTTTTTAAAACAACCGCACTTGCTCTGGTTGTTTCTTCATCAATGCAAAGTTTTGCG
GCACCTACCGACGAAGTAGT

Product: organic solvent tolerance protein precursor

Products: NA

Alternate protein names: Organic solvent tolerance protein

Number of amino acids: Translated: 819; Mature: 819

Protein sequence:

>819_residues
MRQMKYQFKFNPLAAAIFTLLCGGSMQSSYADANDSASSVDNKKLKESIQKAYPGQEFFEQYYVEKSSPEAQVRDTRSLS
SAFCTGTWITPISPTTQAVPADQATSVVTADYAHYNPNGDSELEGNVLIDQQGRSIRANKVTIDRTQTYANAEGNVQLAQ
AGLLAQSDQINYNLKTQQGDLKNSFYISEQQHAHGHAEQIQRTSPTEIILRNATYTTCPPEQKPTWRLEAKEIKLNQDTG
RGTTKNTKLYVKDVPILAVPYFNFPIDNRRTTGILNPNIGFSNDGGLELTVPVYLNLAPNYDATLTPRYISDRGVMLQSE
FRYLTENFGQGKIWGGYLPDDKKYNNEDRKDFNLLHKWKINDYWSTDVEYHYASDKDYVTDLDTNPDSKTDLNLRRAWTL
KYKNQIPGLTAQLKVEDFQTLDKTVSDVDKPYARLPQFLLNYVTGNPLGLQYEFNNDTAYFKKNIDDAANYSTQPSGTRI
YNQFATRYNFRTPWAFAIPEVSIRSINTFYDQNTVENLGLNSDNKSKSVVVPQFSLDTGLIFQRDGDYLQTITPRAFYAY
APYKNQTGYPNFDTTSASINYDQLFSPYRFYGHDRLEDNNFLSLGVSYSLFDPQGLERLRAGVGQSFYFADRRVTLNNTD
DTIDTSKNSGPIVSISSQLTNKFTVAANSAWMSNGDNAQHDFQTYYTGDHGNLYNLGYFNRKNIPDRQLAYDAAVASFVQ
PIMNNWRIMGHVQFDFRNNVAREYLLGVNYESCCYAISVYGRSYYNDLDDPKDPNVNVKRAVMAEITFKGLGGLNNKLAS
LLENRVLGFKEINQSWTQR

Sequences:

>Translated_819_residues
MRQMKYQFKFNPLAAAIFTLLCGGSMQSSYADANDSASSVDNKKLKESIQKAYPGQEFFEQYYVEKSSPEAQVRDTRSLS
SAFCTGTWITPISPTTQAVPADQATSVVTADYAHYNPNGDSELEGNVLIDQQGRSIRANKVTIDRTQTYANAEGNVQLAQ
AGLLAQSDQINYNLKTQQGDLKNSFYISEQQHAHGHAEQIQRTSPTEIILRNATYTTCPPEQKPTWRLEAKEIKLNQDTG
RGTTKNTKLYVKDVPILAVPYFNFPIDNRRTTGILNPNIGFSNDGGLELTVPVYLNLAPNYDATLTPRYISDRGVMLQSE
FRYLTENFGQGKIWGGYLPDDKKYNNEDRKDFNLLHKWKINDYWSTDVEYHYASDKDYVTDLDTNPDSKTDLNLRRAWTL
KYKNQIPGLTAQLKVEDFQTLDKTVSDVDKPYARLPQFLLNYVTGNPLGLQYEFNNDTAYFKKNIDDAANYSTQPSGTRI
YNQFATRYNFRTPWAFAIPEVSIRSINTFYDQNTVENLGLNSDNKSKSVVVPQFSLDTGLIFQRDGDYLQTITPRAFYAY
APYKNQTGYPNFDTTSASINYDQLFSPYRFYGHDRLEDNNFLSLGVSYSLFDPQGLERLRAGVGQSFYFADRRVTLNNTD
DTIDTSKNSGPIVSISSQLTNKFTVAANSAWMSNGDNAQHDFQTYYTGDHGNLYNLGYFNRKNIPDRQLAYDAAVASFVQ
PIMNNWRIMGHVQFDFRNNVAREYLLGVNYESCCYAISVYGRSYYNDLDDPKDPNVNVKRAVMAEITFKGLGGLNNKLAS
LLENRVLGFKEINQSWTQR
>Mature_819_residues
MRQMKYQFKFNPLAAAIFTLLCGGSMQSSYADANDSASSVDNKKLKESIQKAYPGQEFFEQYYVEKSSPEAQVRDTRSLS
SAFCTGTWITPISPTTQAVPADQATSVVTADYAHYNPNGDSELEGNVLIDQQGRSIRANKVTIDRTQTYANAEGNVQLAQ
AGLLAQSDQINYNLKTQQGDLKNSFYISEQQHAHGHAEQIQRTSPTEIILRNATYTTCPPEQKPTWRLEAKEIKLNQDTG
RGTTKNTKLYVKDVPILAVPYFNFPIDNRRTTGILNPNIGFSNDGGLELTVPVYLNLAPNYDATLTPRYISDRGVMLQSE
FRYLTENFGQGKIWGGYLPDDKKYNNEDRKDFNLLHKWKINDYWSTDVEYHYASDKDYVTDLDTNPDSKTDLNLRRAWTL
KYKNQIPGLTAQLKVEDFQTLDKTVSDVDKPYARLPQFLLNYVTGNPLGLQYEFNNDTAYFKKNIDDAANYSTQPSGTRI
YNQFATRYNFRTPWAFAIPEVSIRSINTFYDQNTVENLGLNSDNKSKSVVVPQFSLDTGLIFQRDGDYLQTITPRAFYAY
APYKNQTGYPNFDTTSASINYDQLFSPYRFYGHDRLEDNNFLSLGVSYSLFDPQGLERLRAGVGQSFYFADRRVTLNNTD
DTIDTSKNSGPIVSISSQLTNKFTVAANSAWMSNGDNAQHDFQTYYTGDHGNLYNLGYFNRKNIPDRQLAYDAAVASFVQ
PIMNNWRIMGHVQFDFRNNVAREYLLGVNYESCCYAISVYGRSYYNDLDDPKDPNVNVKRAVMAEITFKGLGGLNNKLAS
LLENRVLGFKEINQSWTQR

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family

Homologues:

Organism=Escherichia coli, GI1786239, Length=754, Percent_Identity=26.657824933687, Blast_Score=224, Evalue=1e-59,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LPTD_ACIAD (Q6F9W4)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046971.1
- STRING:   Q6F9W4
- GeneID:   2880490
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2371
- NMPDR:   fig|62977.3.peg.2258
- eggNOG:   COG1452
- HOGENOM:   HBG289384
- OMA:   TPYYWNI
- PhylomeDB:   Q6F9W4
- ProtClustDB:   CLSK707545
- BioCyc:   ASP62977:ACIAD2371-MONOMER
- HAMAP:   MF_01411
- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543

Pfam domain/function: PF03968 OstA; PF04453 OstA_C

EC number: NA

Molecular weight: Translated: 93137; Mature: 93137

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQMKYQFKFNPLAAAIFTLLCGGSMQSSYADANDSASSVDNKKLKESIQKAYPGQEFFE
CCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHCCCHHHHH
QYYVEKSSPEAQVRDTRSLSSAFCTGTWITPISPTTQAVPADQATSVVTADYAHYNPNGD
HHHCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECEEECCCCC
SELEGNVLIDQQGRSIRANKVTIDRTQTYANAEGNVQLAQAGLLAQSDQINYNLKTQQGD
CCCCCCEEEECCCCEEEECEEEEECCCEECCCCCCEEEEECCCEECCCCCEEEEECCCCC
LKNSFYISEQQHAHGHAEQIQRTSPTEIILRNATYTTCPPEQKPTWRLEAKEIKLNQDTG
CCCCEEECCHHHCCCCHHHHHCCCCCEEEEECCEECCCCCCCCCCEEEEEEEEEEECCCC
RGTTKNTKLYVKDVPILAVPYFNFPIDNRRTTGILNPNIGFSNDGGLELTVPVYLNLAPN
CCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEEECCC
YDATLTPRYISDRGVMLQSEFRYLTENFGQGKIWGGYLPDDKKYNNEDRKDFNLLHKWKI
CCCEECCCEECCCCCEEHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCHHCCEEEEEEC
NDYWSTDVEYHYASDKDYVTDLDTNPDSKTDLNLRRAWTLKYKNQIPGLTAQLKVEDFQT
CCEECCCCEEEECCCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEHHHHHH
LDKTVSDVDKPYARLPQFLLNYVTGNPLGLQYEFNNDTAYFKKNIDDAANYSTQPSGTRI
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCHHHCCCCCCCCHHH
YNQFATRYNFRTPWAFAIPEVSIRSINTFYDQNTVENLGLNSDNKSKSVVVPQFSLDTGL
HHHHHHHCCCCCCEEEECCCEEEEHHHHHHCCCHHHHCCCCCCCCCCEEEEEEECCCCCE
IFQRDGDYLQTITPRAFYAYAPYKNQTGYPNFDTTSASINYDQLFSPYRFYGHDRLEDNN
EEECCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCHHHCCCCCCCCCC
FLSLGVSYSLFDPQGLERLRAGVGQSFYFADRRVTLNNTDDTIDTSKNSGPIVSISSQLT
EEEEEEEEEECCHHHHHHHHHHCCCEEEEECCEEEECCCCCCCCCCCCCCCEEEEHHHHC
NKFTVAANSAWMSNGDNAQHDFQTYYTGDHGNLYNLGYFNRKNIPDRQLAYDAAVASFVQ
CEEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
PIMNNWRIMGHVQFDFRNNVAREYLLGVNYESCCYAISVYGRSYYNDLDDPKDPNVNVKR
HHHHCCEEEEEEEECHHCCHHHHHHCCCCHHHHEEEEEECCCHHHCCCCCCCCCCCCCCE
AVMAEITFKGLGGLNNKLASLLENRVLGFKEINQSWTQR
EEEEEEEHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MRQMKYQFKFNPLAAAIFTLLCGGSMQSSYADANDSASSVDNKKLKESIQKAYPGQEFFE
CCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHCCCHHHHH
QYYVEKSSPEAQVRDTRSLSSAFCTGTWITPISPTTQAVPADQATSVVTADYAHYNPNGD
HHHCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECEEECCCCC
SELEGNVLIDQQGRSIRANKVTIDRTQTYANAEGNVQLAQAGLLAQSDQINYNLKTQQGD
CCCCCCEEEECCCCEEEECEEEEECCCEECCCCCCEEEEECCCEECCCCCEEEEECCCCC
LKNSFYISEQQHAHGHAEQIQRTSPTEIILRNATYTTCPPEQKPTWRLEAKEIKLNQDTG
CCCCEEECCHHHCCCCHHHHHCCCCCEEEEECCEECCCCCCCCCCEEEEEEEEEEECCCC
RGTTKNTKLYVKDVPILAVPYFNFPIDNRRTTGILNPNIGFSNDGGLELTVPVYLNLAPN
CCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEEECCC
YDATLTPRYISDRGVMLQSEFRYLTENFGQGKIWGGYLPDDKKYNNEDRKDFNLLHKWKI
CCCEECCCEECCCCCEEHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCHHCCEEEEEEC
NDYWSTDVEYHYASDKDYVTDLDTNPDSKTDLNLRRAWTLKYKNQIPGLTAQLKVEDFQT
CCEECCCCEEEECCCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEHHHHHH
LDKTVSDVDKPYARLPQFLLNYVTGNPLGLQYEFNNDTAYFKKNIDDAANYSTQPSGTRI
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCHHHCCCCCCCCHHH
YNQFATRYNFRTPWAFAIPEVSIRSINTFYDQNTVENLGLNSDNKSKSVVVPQFSLDTGL
HHHHHHHCCCCCCEEEECCCEEEEHHHHHHCCCHHHHCCCCCCCCCCEEEEEEECCCCCE
IFQRDGDYLQTITPRAFYAYAPYKNQTGYPNFDTTSASINYDQLFSPYRFYGHDRLEDNN
EEECCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCHHHCCCCCCCCCC
FLSLGVSYSLFDPQGLERLRAGVGQSFYFADRRVTLNNTDDTIDTSKNSGPIVSISSQLT
EEEEEEEEEECCHHHHHHHHHHCCCEEEEECCEEEECCCCCCCCCCCCCCCEEEEHHHHC
NKFTVAANSAWMSNGDNAQHDFQTYYTGDHGNLYNLGYFNRKNIPDRQLAYDAAVASFVQ
CEEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
PIMNNWRIMGHVQFDFRNNVAREYLLGVNYESCCYAISVYGRSYYNDLDDPKDPNVNVKR
HHHHCCEEEEEEEECHHCCHHHHHHCCCCHHHHEEEEEECCCHHHCCCCCCCCCCCCCCE
AVMAEITFKGLGGLNNKLASLLENRVLGFKEINQSWTQR
EEEEEEEHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA