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The map label for this gene is Hgd [H]

Identifier: 50085411

GI number: 50085411

Start: 2281421

End: 2282293

Strand: Direct

Name: Hgd [H]

Synonym: ACIAD2316

Alternate gene names: 50085411

Gene position: 2281421-2282293 (Clockwise)

Preceding gene: 50085410

Following gene: 50085412

Centisome position: 63.4

GC content: 42.5

Gene sequence:

>873_bases
ATGATTCAATCAGTTGCATTTATTGGCTTGGGCGCGATGGGCTATCGTATGGCATCGCATTTACCGCGGCACTTCGATAC
AGTTTATGTCTGGAATCGAAGTTTTGAAAAAGCCAAACAACATGCAACTGAATATGGCACACAAGCAGTTGAATTATCTC
AAGCAGTTCAAGCAGACATTATTTTTTCATGTCTACCGACCAGTGCTGATGTAGCACAATTAGTTGAAAATCTTGAATTA
AAACCAGGAACAATTTGGGTCGACTGTACCAGTGGTGTACCAGATTTAGCTCGTCAACTTGTGACTCAACTCAAAAATCA
AGGTGTAGATTATCTTGATGCACCTGTTAGTGGTCAAACCATTGGTGCGGATAATGCAACTTTGACCGTCATGGTGGGTG
GTGATTTCACAGCATTTGAACGTGCCTACCCTGCGATGCAAGCATTTGGCAAGCTGATTAAACATGTGGGTGAGTCTGGT
GCTGGTTTTGCGGTAAAAGCAGTCAATAATATGTTGCTTGCGGTCAACCTCTGGTCAGTTGCCGAAGGCTTTACAACGCT
TAAAGCACATGGAGTGAATCTTGGAGAAGCACTTGATTGTATTAATGCATCAAGCGGTAAAAGTGGTGTGACGGAGACCA
TTTTCCCTCAGCGTGTATTGAATCGTAGTTTTCCTGTTACTTTTGCACTTCCGTTACTTGCCAAAGATGCTGGTATAGCA
CTAGATCTCGTGCATGCGGCAAAACTTCCTGCGCCGTTGATTGCTTTAACTCAAAGTTTAATTCAGGCTGCAAATCAAAC
TGCTGAAGAAAATAGTGATTTTTCTGCTGCTGTGAAAATGTACGAATCGTGGAGCAAAATTACACTTGAGTAA

Upstream 100 bases:

>100_bases
TCACTTAGGGCTAATGTCGAGTCAAGCCAGTTCTGAAGAATTTTGGCCGATGTTTAAGCATTGGTTGGCACAACGTTCAA
CACAAATTCAAGGGTAGATT

Downstream 100 bases:

>100_bases
TTAATCGACATTGAATTTTTTTATAAACACCCTATATTGAGAAATAGGTCGTTACAATTAAATCGACACATAAAGAGGAT
ACGCTCATGAATAAGCTTGC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MIQSVAFIGLGAMGYRMASHLPRHFDTVYVWNRSFEKAKQHATEYGTQAVELSQAVQADIIFSCLPTSADVAQLVENLEL
KPGTIWVDCTSGVPDLARQLVTQLKNQGVDYLDAPVSGQTIGADNATLTVMVGGDFTAFERAYPAMQAFGKLIKHVGESG
AGFAVKAVNNMLLAVNLWSVAEGFTTLKAHGVNLGEALDCINASSGKSGVTETIFPQRVLNRSFPVTFALPLLAKDAGIA
LDLVHAAKLPAPLIALTQSLIQAANQTAEENSDFSAAVKMYESWSKITLE

Sequences:

>Translated_290_residues
MIQSVAFIGLGAMGYRMASHLPRHFDTVYVWNRSFEKAKQHATEYGTQAVELSQAVQADIIFSCLPTSADVAQLVENLEL
KPGTIWVDCTSGVPDLARQLVTQLKNQGVDYLDAPVSGQTIGADNATLTVMVGGDFTAFERAYPAMQAFGKLIKHVGESG
AGFAVKAVNNMLLAVNLWSVAEGFTTLKAHGVNLGEALDCINASSGKSGVTETIFPQRVLNRSFPVTFALPLLAKDAGIA
LDLVHAAKLPAPLIALTQSLIQAANQTAEENSDFSAAVKMYESWSKITLE
>Mature_290_residues
MIQSVAFIGLGAMGYRMASHLPRHFDTVYVWNRSFEKAKQHATEYGTQAVELSQAVQADIIFSCLPTSADVAQLVENLEL
KPGTIWVDCTSGVPDLARQLVTQLKNQGVDYLDAPVSGQTIGADNATLTVMVGGDFTAFERAYPAMQAFGKLIKHVGESG
AGFAVKAVNNMLLAVNLWSVAEGFTTLKAHGVNLGEALDCINASSGKSGVTETIFPQRVLNRSFPVTFALPLLAKDAGIA
LDLVHAAKLPAPLIALTQSLIQAANQTAEENSDFSAAVKMYESWSKITLE

Specific function: Catalyzes the conversion of 2-formylglutarate to (S)-2- hydroxymethylglutarate. Has very low activity with (S)-3- hydroxyisobutyrate [H]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=285, Percent_Identity=29.1228070175439, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI40556376, Length=291, Percent_Identity=27.4914089347079, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI145693186, Length=290, Percent_Identity=34.1379310344828, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1786719, Length=285, Percent_Identity=31.9298245614035, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790315, Length=298, Percent_Identity=27.5167785234899, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1789092, Length=296, Percent_Identity=28.7162162162162, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17557316, Length=276, Percent_Identity=29.3478260869565, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24655230, Length=283, Percent_Identity=30.3886925795053, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19922568, Length=283, Percent_Identity=30.3886925795053, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28574115, Length=214, Percent_Identity=26.6355140186916, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.291 [H]

Molecular weight: Translated: 31019; Mature: 31019

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSVAFIGLGAMGYRMASHLPRHFDTVYVWNRSFEKAKQHATEYGTQAVELSQAVQADI
CCCCCHHHHHHHHHHHHHHHCCHHCCEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHH
IFSCLPTSADVAQLVENLELKPGTIWVDCTSGVPDLARQLVTQLKNQGVDYLDAPVSGQT
HHHCCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCE
IGADNATLTVMVGGDFTAFERAYPAMQAFGKLIKHVGESGAGFAVKAVNNMLLAVNLWSV
ECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCEEEEEEHHHH
AEGFTTLKAHGVNLGEALDCINASSGKSGVTETIFPQRVLNRSFPVTFALPLLAKDAGIA
HCCHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHCCCCCH
LDLVHAAKLPAPLIALTQSLIQAANQTAEENSDFSAAVKMYESWSKITLE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEECC
>Mature Secondary Structure
MIQSVAFIGLGAMGYRMASHLPRHFDTVYVWNRSFEKAKQHATEYGTQAVELSQAVQADI
CCCCCHHHHHHHHHHHHHHHCCHHCCEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHH
IFSCLPTSADVAQLVENLELKPGTIWVDCTSGVPDLARQLVTQLKNQGVDYLDAPVSGQT
HHHCCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCE
IGADNATLTVMVGGDFTAFERAYPAMQAFGKLIKHVGESGAGFAVKAVNNMLLAVNLWSV
ECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCEEEEEEHHHH
AEGFTTLKAHGVNLGEALDCINASSGKSGVTETIFPQRVLNRSFPVTFALPLLAKDAGIA
HCCHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHCCCCCH
LDLVHAAKLPAPLIALTQSLIQAANQTAEENSDFSAAVKMYESWSKITLE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA